1.A more sensitive method for the evaluation of visceral pain sensitivity in rat.
Yu CHEN ; Chun LIN ; Ai-Qin CHEN
Chinese Journal of Applied Physiology 2009;25(1):54-59
Abdominal Muscles
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physiology
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Abdominal Pain
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diagnosis
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physiopathology
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Animals
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Animals, Newborn
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Colon
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innervation
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Electrodes
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Electromyography
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Female
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Pain Measurement
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methods
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Pain Threshold
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physiology
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Rats
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Rats, Sprague-Dawley
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Viscera
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Visceral Afferents
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physiology
2.Developmental Characters of Neural Stem Cells in Occipital of Cortex from Human Fetal Brain at Different Ages
bo, HU ; ai-hua, LI ; yu-lin, AN ; zhi-chun, FENG
Journal of Applied Clinical Pediatrics 2006;0(14):-
Objective To investigate the developmental characters of neural stem cells(NSCs) in occipital of cortex from human fetal brain at different age.Methods Ninety cases of embryoes at gestational age 16-32 weeks and by induction of labor with water bag were collected for determining distribution,shapes,growth modes and the number of NSCs in the occipital of cortex with immunohisto- chemical method under light microscope.Results It was noted that NSCs existed in the occipital of cortex from human fetal brain at different ages.NSCs mainly distributed in layers of cone cells and inner granule cells.NSCs existed in the occipital of cortex of different fetal age included middling round cells,NSCs had enations from 0 to 1.Nucli were larger than plasm.Each NSC had nucleoli from 2-4 and rarefaction chromatin.Most of NSCs distributed in three growth modes including crowd,cluster and clone,occasionally with a single growth mode among other nerve cells.There were no differences including distribution,shapes,growth modes and the number of NSCs in the occipital of cortex between groups,but,NSCs gradually decreased with increasing of age.Conclusion NSCs exists in the occipital of cortex from different gestational age,and the number of NSCs decreases with increasing of age.
3.Dipylidium caninum infection in a child.
Yu-liang ZHAO ; Xi-meng LIN ; Ai-ying ZHAI
Chinese Journal of Pediatrics 2008;46(4):311-311
Cestode Infections
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parasitology
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Humans
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Infant
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Male
4.The study of siRNA interference after laryngeal cancer Hep-2 cells to cisplatin sensitivity of β-catenin gene expression.
Feng YU ; Xin HUANG ; Maomao AI ; Ying LIN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(13):1143-1147
OBJECTIVE:
To investigate the changes of laryngeal cancer Hep-2 cells to cisplatin chemosensitivity after the interference of siRNA of β-catenin gene expression.
METHOD:
Using a small interference RNA (siRNA) technology interfere β-catenin gene of Hep-2 cells . The mRNA and protein levels of β-catenin in the Hep-2 cells of different groups were detected by qPCR and Western blot. It was divided into siRNA-β-catenin-Hep-2 siRNA group, β-catenin-Neg negative control group and blank control group. Cell proliferation inhibition rate of different concentrations of cisplatin on three groups was detected by MTT assay. Calculate the 50% inhibitory effective concentration IC50 value. Check the change of three groups of cells' apoptosis rate by flow cytometry after the same concentrations of cisplatin stimulation.
RESULT:
β-catenin-siRNA interference fragment can specifically reduce the expression levels of β-catenin mRNA and protein. qPCR illustrated the expression of mRNA in β-catenin-siR-NA-Hep-2 interference group decreased 70% (P < 0.05) compared with the control group, Western blot results showed that the β-catenin protein expression of interference group (0. 545 ± 0.111) decreased significantly compared with blank control group (1.507 ± 0.139) and negative control group (1.429 ± 0.089), P < 0.05. The IC50 calculation software showed that IC50 of cisplatin on β-catenin-siRNA IC50 interference group is (5.81 ± 0.46)μg/ml, the blank control group is (10.10 ± 1.01) μg/ml, the difference between the two groups has statistical signifi- cance (P < 0.01). Cell apoptosis rate of β-catenin-siRNA interference group was (26.15 ± 0.60)%, significantly higher than the control group (14.16 ± 0.05)%, P < 0.05.
CONCLUSION
To interfere the expression of β-catenin can effectively enhance the sensitivity of laryngeal cancer cells to chemotherapeutic drugs cisplatin. It provides a theoretical support for the reduction of laryngeal cancer chemotherapy drug cisplatin dosage.
Apoptosis
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Cell Line, Tumor
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Cisplatin
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pharmacology
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Drug Resistance, Neoplasm
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Humans
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Laryngeal Neoplasms
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genetics
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RNA Interference
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RNA, Messenger
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RNA, Small Interfering
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beta Catenin
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genetics
5.Methylation status of promoter of mismatch repair genes hMLH1 and hMSH2 in epithelial ovarian cancer
Shi-Qian ZHANG ; Ai-Feng ZHANG ; Lin-Lin ZHANG ; Le-Le FU ; Hao YU ;
Chinese Journal of Laboratory Medicine 2000;0(06):-
Objective To explore the methylation status of hMLH1 and hMSH2 promoter region in the epithelial ovarian cancer and its role in oncogenesis.Methods Methylation status of hMLH1 and hMSH2 promoter region was assayed in 20 normal ovarian tissues,25 benign epithelial tumor,56 malignant epithelial tumor and cell lines SKOV3,3AO by methylation-specific PCR (MSP).SKOV3 and 3AO were analyzed before and after 5-aza-2′-deoxycytidine (5-Aza-CdR) treatment.In addition,an alterations of mRNA expression of hMLH1 and hMSH2 was observed by reverse transcription polymerase chain reaction (PT-PCR).Results No methylation of hMLH1 and hMSH2 promoter was found in normal ovarian tissues. CPG islands methylation of hMLH1 and hMSH2 was observed in 4% (1/25),8% (2/25) respectively in benign epithelial tumor,30.4% (17/56),51.8% (29/56) respectively in malignant epithelial tumor. Methylation status in promoter showed obvious correlation with pathological grade and lymph node metastasis (P
6.Relationship between oral and gastric Hp infection and diseases of upper digestive tract in children.
Yun-guang BAO ; Yi WEI ; Ai-su LANG ; Wei-jun YU ; Ai-juan YING ; Lin-qing MU ; Hui-xian YANG
Chinese Journal of Pediatrics 2003;41(6):465-466
Adolescent
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Child
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Child, Preschool
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Female
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Gastric Mucosa
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microbiology
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Gastrointestinal Diseases
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diagnosis
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microbiology
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Helicobacter Infections
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diagnosis
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microbiology
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Helicobacter pylori
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growth & development
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isolation & purification
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Humans
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Infant
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Male
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Mouth Mucosa
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microbiology
7.Helicobacter pylori infection and its related diseases.
Yu ZHAO ; Xiao-Hua XU ; Feng-Lin LIU ; Shu-Hong ZHANG ; Ai-Ming SITU
Chinese Journal of Contemporary Pediatrics 2008;10(3):403-404
Adolescent
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Bile Reflux
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etiology
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Child
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Child, Preschool
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Female
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Gastritis
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etiology
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Gastroscopy
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Helicobacter Infections
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complications
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diagnosis
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Helicobacter pylori
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Humans
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Male
8.Researching on fingerprint of Inulacappa by HPLC.
Di-fei-fei XIONG ; Di ZHU ; Dan TAN ; Yan-yu LAN ; Lin ZHENG ; Ai-min WANG
China Journal of Chinese Materia Medica 2015;40(3):480-483
OBJECTIVEThis study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC.
METHODThe HPLC analysis was performed on an Agilent Eclipse Plus C18 column (2.1 mm x 150 mm, 1.8 μm) with 0.1% fomic acid aqueous solution-0.1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0.3 · mL(-1) · min(-1); The detective wavelength is 325 nm; The column temperature is 45 °C.
RESULTThe results indicated that 5 of 17 common peaks were identified . The similarity about 10 groups of Inulacappais is over 0.95.
CONCLUSIONThis method is able to be a scientific basis of quality assessment according to its convenient and reliable.
Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Inula ; chemistry
9.Occlusion the posterior semicircular canal using laser for treatment the complex benign paroxysmal positional vertigo.
Shun-Zhang LIN ; Ai-hua SUN ; Shu-Chang TIAN ; Peng-Yu CAO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(1):77-77
Female
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Humans
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Laser Therapy
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methods
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Middle Aged
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Semicircular Canals
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surgery
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Vertigo
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etiology
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surgery
10.Liangge san effects the expression of CD14 and scaverger receptor in the Kupffer cells of liver of endotoxemia mice.
Lin-Zhong YU ; Ai-Da JIANG ; Yu-Yao CHENG ; Hui LIN ; Qing-He QIN ; Xiao-Dong MA
China Journal of Chinese Materia Medica 2006;31(3):220-223
OBJECTIVETo study the effects of Liangge San to the expression of CD14 and scaverger receptor(SR) in the kupffer cells of liver and the pathological changes of liver tissue of endotoxemia-mice.
METHODThe model was established with intravenous injection of lipopolysaccharide (LPS) and at the same time different dose Liangge San were given. The expression of CD14 and scaverger receptor were detected with immunohigtochemistry at the 2nd, 4th, 8th hour ofter injury and analyzed with computer image system, and the pathological changes of liver tissue were also observed.
RESULTAt the three different hours, the expression of CD14 and scaverger receptor in macrophages of liver of LPS-injury group showed significant increase and significant decrease respectively, compared with that of the blank-control group (P < 0.01). The expression in dexamethasone group and Liangge San different dose groups were intermediate between those in injury group and those in control group. Compared with expression of LPS-injury group, those of dexamethasone group and Liangge San different dose groups showed significant differences (P < 0.01), especially that of Liangge San high dose group. Liver cells showed vacuole change. Changes of CD14 and SR expression were paralleled with the severity of liver damages of the mice.
CONCLUSIONLiangge San can inhibite the up-regulation of CD14 expression and down-regulation of scaverger receptor expression in a dosage-dependent manner and also alleviate the damages of liver induced by LPS.
Animals ; Anti-Inflammatory Agents ; pharmacology ; Dexamethasone ; pharmacology ; Dose-Response Relationship, Drug ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; pharmacology ; Endotoxemia ; chemically induced ; metabolism ; pathology ; Female ; Kupffer Cells ; metabolism ; pathology ; Lipopolysaccharide Receptors ; metabolism ; Lipopolysaccharides ; Liver ; metabolism ; pathology ; Mice ; Plants, Medicinal ; chemistry ; Receptors, Scavenger ; metabolism