China ; Humans ; Medicine ; methods ; trends ; Medicine, Chinese Traditional ; trends

Arthritis, Rheumatoid ; diagnosis ; therapy ; Diagnosis, Differential ; Humans ; Integrative Medicine ; methods ; trends ; Medicine, Chinese Traditional ; methods ; trends ; Professional Practice ; trends ; Therapeutics ; methods ; trends ; Treatment Outcome

The disease classification in Western medicine and syndrome differentiation in traditional Chinese medicine were comparatively analyzed in this paper. It is proposed that the research on syndrome differentiation of disease classification is one of the cut-in points in integrative medicine research, and indicated that the research may be deepened by way of TCM syndrome classification based on diagnosis and on therapeutic efficacy.
Diagnosis, Differential ; Disease ; classification ; Humans ; Medicine, Chinese Traditional ; methods ; trends ; Research Design

The recombinant fusion protein staphylokinase-hirudin(rSFH) was purified from the high density-fermented engineered E.coli by means of ion-exchange chromatography (IEC) and gel filtration (GF). The purity of rSFH reached to more than 98% determined by RP-HPLC and SDS-PAGE, and the yield was up to 0.7g per liter of fermentation broth. The analysis of homologous dimmer of rSFH appeared during the purification and calculation of the surface hydrophobic area had been carried out by means of hydrophobic chromatography and MALD-TOF. The influence of sodium chloride and temperature on the behavior of rSFH reversible dimerization was analyzed by high performance sized- exclusive chromatography(HPSEC). It is concluded that the hydrophobic interaction played an important role in the reversible dimerization of rSFH.

AIM: To observe the role of c-fos in the protection by cholecystokinin-octapeptide (CCK-8) against pulmonary artery smooth muscle cell (PASMC) injury induced by lipopolysaccharide (LPS). METHODS: The ultrastructure of PASMC was observed under transmission electron microscope (TEM). MDA content,LDH release and the rate of trypan blue in PASMC were measured,and immunocytochemistry technique was adopted to observe the c-fos protein expression. RESULTS: The TEM results showed significant PASMC structural injury in LPS group and alleviated structural changes in LPS+CCK-8 group. CCK-8 reduced the increase in the rate of trypan blue uptake,MDA content and LDH release in PASMC induced by LPS. LPS lightly increased c-fos protein expression,which was enhanced by CCK-8. CONCLUSION: CCK-8 attenuated the injury of PASMC induced by LPS,which may be concerned with the increase in c-fos protein expression.

Integrative medicine (IM), usually called integrated traditional and Western medicine in China, which came into being in the 1950s as a new form of medicine, although is now developing quickly, little is known about its status and existing problems. So a national survey in China was conducted through questionnaire in 2004 by Chinese Association of Integrative Medicine with the support of State Administration of Traditional Chinese Medicine (SATCM). The results show that, of all the medical professionals investigated in this survey, 91.21% and 93.52% respondents respectively favored IM as the best diagnostic and therapeutic method. Of all the patients who once went through TCM, Western medicine (WM) and IM therapies, 68.85%, 65.45% and 71.2% respondents respectively most appreciated IM, IM hospitals and IM therapeutic treatments. Most of the 6 595 respondents held that the optimal scientific research strategy in TCM should be integrating modern medical research method (n = 2 380) or modern scientific method (n = 2 920). However, many hospitals exposed the problems in the aspects of governmental supports and funding supports, human resources, and domestic or international academic activities. These results indicated that IM is the patients' social needs and doctors' aspiration in China. For further development of IM, the enhancement of scientific research construction and assistance by policies and finance from the government and other institutions are urgently needed in China.
Attitude of Health Personnel ; China ; Delivery of Health Care, Integrated ; Health Care Surveys ; Humans ; Medicine, Chinese Traditional ; National Health Programs ; Patient Satisfaction ; Physicians ; psychology ; Surveys and Questionnaires

ObjectiveTo explore the reliability and validity of WHO ICF-Clinical Checklist for patients with spinal cord injuries.MethodsClinical testing, case records and check had been implemented for 50 patients with spinal cord injuries with ICF-Clinical Checklist, ADL and ASIA.Results and ConclusionThere were high test-retest reliability and validity in WHO ICF-Checklist for patients with spinal cord injuries.

In order to realize the current situation and existing problems of integrative medicine of Chinese and Western medicine (ICWM) in China, a survey with questionnaires was conducted, its contents including the viewpoint of patients, who received ICWM therapy, on ICWM and on doctors of ICWM; the condition of ICWM researches; the present development of ICWM hospital and the existing problem in these hospital, etc. by inquiry on medical workers showed that most of them considered the best approach for medical research is the modern medical or the modern scientific studying method, and more than 71.2% patients prefered ICWM therapy the treatment most willing to receive.
Attitude of Health Personnel ; China ; Delivery of Health Care, Integrated ; methods ; Health Care Surveys ; Humans ; Medicine, Chinese Traditional ; Patient Satisfaction ; Surveys and Questionnaires

Evidence-Based Medicine ; Integrative Medicine ; standards ; Medicine, Chinese Traditional ; standards ; Practice Guidelines as Topic ; standards ; Practice Patterns, Physicians' ; standards

Objective　To observe some biological features of bone marrow mesenchymal stem cells and explore the best conditions for isolatin g and culturing in vitro. Methods　Common cell culture techn ique, light and electron microscopy were used to study the effects of the growth , proliferation, morphology of the bone marrow mesenchymal stem cells in differe nt adherent time, concentration of serum and cell density. Results　The best culture condition in vitro for growth was 4-24 hours adherent time, 5%-10% fetal bovine serum, (4-8)×104/ml cell density. The cells were sp indle in shape and had a strong ability of proliferation. The time for cell duplication was 3 to 4 days. The cells showed the characteristics of stem cell s in electron microscope. Conclusion　The best condition for iso lation and culture of bone marrow mesemchymal stem cells was successfully establ ished and some biological features were obserred. It found a base for further in vestigation and using of mesenchymal stem cells.