1.Influence of seedling grade on plant growth, yield and quality of Anoectochilus roxburghii.
Qing-Song SHAO ; Ai-Cun ZHOU ; Run-Huai HU ; Yu-Yun ZHANG ; Tang-Mei LIU ; Ming-Yan LI
China Journal of Chinese Materia Medica 2014;39(5):785-789
The morphological index of the seedlings including the plants height, the ground diameter, the leaf amounts, the fresh weight of the whole plant and the ratio of height to diameter was measured and the principal components were analyzed so as to determine the grading index, and stepwise cluster analysis was applied for clustering analysis. Pot experiments were used to measure the indicators of plant growth and development, the yield and the quality. The results showed that the height and ground diameter were determined as the quality indicators of the seedlings grading and the standard quality grading of seedlings of Anoectochilus roxburghii was initially set up, different seeding plants influenced the plants growth and the yield. The ground diameter of the class I was larger than that of the class II and III, so as the yield. The seedling grading had no obvious effect on the internal quality of medicinal materials. The results of the study provide the basis for standard cultivation of A. roxburghii.
Drugs, Chinese Herbal
;
analysis
;
Orchidaceae
;
chemistry
;
classification
;
growth & development
;
Quality Control
;
Seedlings
;
chemistry
;
classification
;
growth & development
2.Effects of different transplanting conditions on survival rate and growing status of Anoictochilus roxburghii plantlets.
Qing-Song SHAO ; Ai-Cun ZHOU ; Yu-Qiu HUANG ; Ying-Lei DONG ; Bing-Kang HU ; Ming-Yan LI
China Journal of Chinese Materia Medica 2014;39(6):955-958
The growing status of Anoectochilus roxburghii seedling was observed and the survival rate of seedlings, height, stem diameter and plant fresh weight under the conditions of different transplanting substrate compositions, planting density, shading rate were measured. The results showed that the effects of different transplanting substrates, planting densities, shading rates and nutrient solutions on the growing status of A. roxburghii plantlets varied greatly. A. roxburghii plantlets demonstrated a high survival rate and better growing status under the Following conditions: the ratio of peat and river sand as 2: 1, the planting density as 3 cm x 3 cm, the shading rate as 70%, and the nutrient solution as 1/4MS. The findings of the study provide a solid technical solution for the artificial cultivation of A. roxburghii plantlets.
Breeding
;
methods
;
Culture Media
;
chemistry
;
pharmacology
;
Orchidaceae
;
drug effects
;
growth & development
;
Seedlings
;
drug effects
;
growth & development
;
Survival Analysis
3.Mycena subpiligera sp. nov., a Symbiotic Species from China Associated with the Seed Germination of Gastrodia elata
Li-na LIU ; Guo-ying ZHOU ; Ai-rong SHEN ; Bao-ming SHEN ; Yun TAN ; Zhu-ming TAN
Mycobiology 2022;50(5):294-301
Mycena subpiligera, a new taxon in sect. Fragilipedes that can strongly enhance the germination efficiency of Gastrodia elata seeds, was discovered in subtropical areas of China. As revealed by a morphological comparison with related Mycena species as well as maximum likelihood (ML) and Bayesian phylogenetic analyses based on sequences of the internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA, the new taxon can be distinguished from phenotypically similar and phylogenetically related species. Optimal cultural conditions for M. subpiligera basidiomata are reported, and the germination rate of the new species is compared with that of M. citrinomarginata.
4.Synergistic immunomodulatory effects of interferon-gamma and bone marrow mesenchymal stem cells.
Chen LIANG ; Shu-lian CHEN ; Mei WANG ; Wen-jing ZHAI ; Zheng ZHOU ; Ai-ming PANG ; Si-zhou FENG ; Ming-zhe HAN
Chinese Journal of Hematology 2013;34(3):213-216
OBJECTIVETo investigate mesenchymal stem cells (MSCs) immunosuppressive activity in the presence of interferon-gamma (IFN-γ) to reveal synergistic immunomodulatory effects of IFN-γ and MSCs.
METHODS① MSCs were cultured in the presence or absence of IFN-γ(100 ng/ml), the supernatants were collected for measurements of PGE2、HGF and TGF-β1 by ELISA kits. ② MSCs were cultured in the presence or absence of IFN-γ (100 ng/ml)for 48 h. The cDNA was analysed for the expression of human indoleamine 2, 3-dioxygenase(IDO)mRNA by semiquantitative RT-PCR. ③ Mononuclear cells (MNCs) were extracted from peripheral blood of healthy donors. The T cell proliferation was tested in the co-culture system added with MSCs, recombinant human IFN-γ (100 ng/ml) and anti-IFN-γ mAb (5 μg/ml) by BrdU ELISA kit.
RESULTS①The immunosuppressive cytokines PGE2、HGF and TGF-β1 were detectable within 24-48 h in the supernatants. Their expressions were significantly up-regulated in the presence of IFN-γ. Concentrations of these cytokines were as of (1715.5±628.6) pg/ml vs (1344.5±709.4) pg/ml (P=0.001);(4031.8±1496.8) pg/ml vs (2452.4±1375.3) pg/ml(P=0.011);(1753.5±413.8) pg/ml vs (1026.6±450.5) pg/ml(P<0.001),respectively. ②The expression of IDO mRNA was undetectable when MSCs were cultured alone. In contrast, The IDO mRNA expression was remarkably enhanced in the presence of IFN-γ. ③Bone marrow-derived MSCs remarkably suppressed allogeneic T cell proliferation in vitro. Addition of exogenous IFN-γ had no significant effect on the inhibitory capacity of MSCs, the inhibitory ratios of T cell proliferation were (40.4±10.9)% vs(36.7±7.4)% (P=0.272). By contrast, the inhibitory ratio of T cell proliferation was significantly decreased in the presence of anti-IFN-γ mAb[(40.4±10.9)% vs (23.9±7.6)%,P=0.002].
CONCLUSION①Human MSCs constitutively expressed immunosuppressive concentrations of PGE2, HGF and TGF-β1, and their expressions were significantly up-regulated by IFN-γ. ②IFN-γ-induced expression of IDO on MSCs involved in tryptophan catabolism. ③MSCs notably suppressed allogeneic T cell proliferation in vitro. IFN-γ promoted the immunosuppressive capacity of human MSCs, indicating the synergistic immunomodulatory effect of IFN-γ and MSCs.
Bone Marrow Cells ; immunology ; Cell Proliferation ; Cells, Cultured ; Coculture Techniques ; Cytokines ; immunology ; Humans ; Immune Tolerance ; Interferon-gamma ; pharmacology ; Mesenchymal Stromal Cells ; immunology ; T-Lymphocytes ; cytology
5.Repairing tibial post-traumatic osteomyelitis with bone and skin defect by Ilizarov technique at stage I.
Jiang SHOU-HAI ; Dong-xin QIU ; Chang-hong DONG ; Ming-liang XU ; Liang HAO ; Ye ZHANG ; Li-guo ZHOU ; Jian-jun XIA ; Ai-min PENG
China Journal of Orthopaedics and Traumatology 2015;28(12):1125-1128
OBJECTIVETo explore clinical effects of Ilizarov technique at stage I for repairing tibial post-traumatic osteomyelitis with bone and skin defect.
METHODSFrom June 2010 to December 2013,44 patients with tibial post-traumatic osteomyelitis with bone and skin defect were treated with Ilizarov technique at stage I . Among them, there were 35 males and 9 females aged from 18 to 70 years old with an average of 42.5 years old. Bone defect ranged from 4 to 16 cm, skin defect ranged from 3 cm x 4 cm to 5 cm x 16 cm. The operation was performed debridement thoroughly, removed inflammatory bone section, osteotomy invasively, install circular external fixator by Ilizarow technique; screw nut were rotated at 1 week after operation, and prolonged 0.5 to 1.0 mm everyday. Wound surface, new born callus and bone healing were observed to evaluate clinical effects.
RESULTSAll patients were followed up from 11 to 36 months with an average of 18.5 months. Bone defect after osteotomy was from 6 to 22 cm with an average of 11.5 cm; the time of wound healing time ranged from 21 to 79 d with an average of 38 d; bone defect healing time was from 8 to 15 months with an average of 12.5 months. All patients were cured, no recurrent infection, refracture and shorten of calf deformity were occurred.
CONCLUSIONRepairing tibial post-traumatic osteomyelitis with bone and skin defect by llizarov technique at stage I has advantages of less trauma, low inflammatory recurrence rate, could avoid multiple complex operation, and receive definite curative effect.
Adolescent ; Adult ; Aged ; Female ; Humans ; Ilizarov Technique ; Male ; Middle Aged ; Osteomyelitis ; surgery ; Osteotomy ; Tibia ; surgery
6.Effects of MSF on melanocyte adhesion and migration in vitro.
Kuan-hou MOU ; Xian-qi ZHANG ; Bing YU ; Ai-ming ZHOU ; Jie FENG
China Journal of Chinese Materia Medica 2004;29(4):346-349
OBJECTIVETo investigate the Malytea Scurfpea fruit (MSF) on melanocyte adhesion and migration.
METHODHuman epidermal melanocytes were treated with MSF and Ginger respectively, then adhesion to bovine serum fibronectin-coated culture dishes was checked. Control and treated cells were also examined for migration into micropore filters coated with the same protein.
RESULTCompared with control, MSF treated melanocytes were obviously easier to adhere to the dishes and move into the filters in a dose-dependent manner. When the dose of MSF was 200 mg x L(-1), it could not reincrease melanocyte adhesion and migration. At 10 mg x L(-1), under every other concentrations of MSF, there was no marked difference among MSF-treated, Ginger-treated and untreated melanocytes (P < 0.05) when adhesion test were studied. But to migration, even at 10 mg x L(-1) MSF, there was obvious increased migration compared with MSF-untreated or Ginger-treated melanocytes (P < 0.01).
CONCLUSIONMSF has effect on melanocyte adhesion and migration, which can explain, in part, the capacity of MSF to modulate melanocyte function in vitiligo lesions.
Cell Adhesion ; drug effects ; Cell Movement ; drug effects ; Cells, Cultured ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Fruit ; chemistry ; Ginger ; chemistry ; Humans ; Melanocytes ; cytology ; drug effects ; Plants, Medicinal ; chemistry ; Psoralea ; chemistry
7.Comparative study of the factors in tuberculosis treatment between immigrant workers and local residents in Shenzhen.
Jin-quan CHENG ; Ying-zhou YANG ; Rui-qian XIE ; Wen-ming ZHONG ; Ai-guo TAN ; Yu-xi LUO ; Zu-xun LU
Chinese Journal of Preventive Medicine 2009;43(2):141-145
OBJECTIVETo study the factors affecting the management and treatment of immigrant workers with tuberculosis, to determine the most effective measures and to provide an academic basis for tuberculosis control and prevention strategies targeting the immigrant population.
METHODSA self-designed questionnaire was administered to 1364 immigrant workers with tuberculosis (study group) and 436 local residents (control group) with tuberculosis.
RESULTSWhether a patient's tuberculosis status was discovered or not was related to the medical facilities initially visited, job mobility, and the individual worker's economic condition. The percentage of cases discovered was relatively low among those who made their first visit to a private clinic (39.0%), and 58.4% (796) of immigrant workers delayed their first consultation mainly due to neglecting symptoms (55.9%, 445), subjective perception of symptoms being not severe enough (19.3%, 154) to visit a physician and the inconvenience of visiting a hospital because of its being far away from home (15.8%, 47). As comparing immigrant workers with local residents, the differences of the delay reason between the two groups were statistically significant (chi(2) = 21.49, P < 0.01). And 39.9% (544) of immigrant workers and 49.1% (214) of local residents had had late confirmation. The differences of the delay reasons between the two groups were insignificant (chi(2) = 7.31, P = 0.293). And 17.6% (240) patients of immigrant workers and 13.1% (57) patients of local residents did not keep to their drug regimens in a timely fashion. The differences of the reasons between the two groups were insignificant (chi(2) = 6.66, P > 0.05). And 77.5% (1057) of immigrant workers and 31.8% (138) of local residents were considered that taking medicine in supervision spot might have impact on their lives and work. The differences of the reasons between the two groups were significant (chi(2) = 9.71, P < 0.05). All 79.2% (1080) of immigrant workers and 63.3% (276) of local residents did not obtain medicine according to prescriptions. The differences of reasons between the two groups were statistically significant (chi(2) = 24.84, P < 0.01). And 51.2% (699) of immigrant workers and 46.1% (201) of the registered population did not follow up with doctors' directions for lab tests. The differences of the reasons between the two groups were insignificant (chi(2) = 3.26, P > 0.05).
CONCLUSIONThe influential factors in tuberculosis management and treatment were complex. Prevention strategies should focus on health education and promotion activities to improve awareness in seeking medical services. Also, developing and standardizing reference mechanisms for patients, and alleviating the economic burden of the workers will be critical to reduce the tuberculosis incidence.
China ; epidemiology ; Factor Analysis, Statistical ; Humans ; Transients and Migrants ; Treatment Outcome ; Tuberculosis, Pulmonary ; epidemiology ; prevention & control ; therapy ; Urban Population
8.Sperm DNA damage and unexplained recurrent spontaneous abortion.
Cheng-Jun LIU ; Ai-Ming WANG ; Wei SHANG ; Min LI ; Wei-Zhou WANG ; Yu-Liang SHEN ; Yong ZHAO
National Journal of Andrology 2011;17(7):619-621
OBJECTIVETo explore the relationship of sperm DNA damage with unexplained recurrent spontaneous abortion (URSA).
METHODSSperm DNA fragmentation was evaluated by sperm chromatin dispersion (SCD), and the results were expressed in terms of DNA fragmentation index (DFI). DFIs were measured in the male partners of 56 women with URSA (the experiment group) and of 31 without URSA (the control group).
RESULTSThe DFI was shown to be (11.0% - 56.9%) in the experiment group, 21 (37.5%) of the subjects over 30%, as compared with (10.0% -36.8%) in the control group, only 8 (25.8%) of the subjects over 30%, significantly higher in the former than in the latter (29.4% vs 25.5%, P < 0.05).
CONCLUSIONThere is a relationship between sperm DNA damage and URSA.
Abortion, Habitual ; etiology ; Adult ; Case-Control Studies ; DNA Damage ; DNA Fragmentation ; Female ; Humans ; Male ; Pregnancy ; Spermatozoa ; pathology
9.Action of beta-amyloid peptide₁₋₄₀ on I(HVA) and its modulation by ginkgolide B.
Lei CHEN ; Chang-Jin LIU ; Ming TANG ; Ai LI ; Xin-Wu HU ; Ying ZHOU ; Jurgen HESCHELER
Acta Physiologica Sinica 2006;58(1):14-20
Whole-cell patch clamp recording was used to investigate the action of beta-amyloid peptide(1-40) (Abeta(1-40)) on high voltage-activated calcium channel current (I(HVA)) in acutely isolated hippocampal CA1 pyramidal neurons in rats and observe its modulation by ginkgolide B (GB). Drug was applied by extracellular bath or adding in the pipette solution, and its effect was determined by comparing the amplitude of I(HVA) before and after the drug application. Bath application of aggregated Abeta(1-40) at concentrations of 0.01~30 mumol/L increased the amplitude of I(HVA) in a dose-dependent manner by (5.43+/-3.01)% (n=8, P>0.05), (10.49+/-4.13) % (n=11, P>0.05), (40.69+/-8.01) % (n=16, P<0.01), (58.32+/-4.85) % (n=12, P<0.01), and (75.45+/-5.81) % (n=6, P<0.01), respectively, but had no effect on the I-V curve of I(HVA); fresh Abeta(1-40) almost had no effect on I(HVA) (n=5, P>0.05). L-type calcium channel antagonist nifedipine abolished the increase of I(HVA)by Abeta(1-40). The increase of I(HVA) by Abeta(1-40) (1.0 mumol/L) was enhanced to (66.19+/-5.74) % (P<0.05) by 8-Br-cAMP (membrane permeable analogue of cAMP) and to (73.21+/-6.90) % (P<0.05) by forskolin, an adenylyl cyclase (AC) agonist, and reduced to (20.08+/-2.18) % (P<0.05) by H-89, cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) antagonist. GB effectively inhibited the increase of I(HVA) by Abeta(1-40). The results indicate that Abeta(1-40) leads to an intracellular calcium overload by increasing I(HVA) via AC-cAMP-PKA. This may be one of the mechanisms for its neurotoxicity. GB can prevent neurons from neurotoxicity by inhibiting abnormal calcium influx caused by Abeta(1-40).
Amyloid beta-Peptides
;
toxicity
;
Animals
;
Animals, Newborn
;
Calcium Channels
;
drug effects
;
Ginkgolides
;
pharmacology
;
Hippocampus
;
cytology
;
metabolism
;
Lactones
;
pharmacology
;
Neurons
;
drug effects
;
metabolism
;
Neuroprotective Agents
;
pharmacology
;
Patch-Clamp Techniques
;
Peptide Fragments
;
toxicity
;
Rats
;
Rats, Wistar
10.Correlation between suicide ideation and negative life events among college students
Jianmei CHEN ; Xueying ZHOU ; Ming AI ; Yajuan NIU ; Lipeng FEI ; Li KUANG
Chongqing Medicine 2017;46(30):4226-4228
Objective To understand the occurrence situation of negative life events in the past year among college students with suicidal ideation and to explore the correlation between negative life events and suicidal ideation. Methods The stratified and cluster sampling method was applied to conduct the questionnaire survey on 12 114 students from 11 universities in Chongqing City. The questionnaire of general demographic information was used to learn sociodemographic characteristics;the perspective on personal life questionnaire was applied to evaluate the suicidal ideation within recent 1 month and the self-designed life event scale was used to investigate the occurrence of negative life events in the past 1 year. Results The report rate of suicidal ideation within 1 month was 9.6%, the occurrence rate of short term suicidal ideation was increased with experiencing more negative life events. The x2 test showed that there was statistically significant difference in the proportion of suicidal ideation occurrence between college students experiencing 1 negative life event and those without experiencing the negative life event (x2 = 37.72,P<0.01) ;besides, the occurrence proportion of recent suicidal ideation had statistical difference between the college students with experiencing two or more negative life events and those without experiencing negative life event (x2 = 65.46,P<0.01). The multivariate Logistic regression analysis showed that the conflict with other family members (OR=0. 295,95%CI 0. 017-0. 571),abuse (OR=7. 295, 95%CI 6. 049-9. 701) were independentrisk factor for the recent suicidal ideation in college students. Conclusion Experiencing more negative life events, conflicting with other family members and abused by others have a correlation with the appearance of short term suicidal ideation in college students.