Objective To investigate the activation mechanism of matrix metalloproteinase-13 zymogen (pro-MMP-13) induced by nitric oxide (NO). Methods Human chondrosarcoma cells (SW1353) were grouped and treated with the NO donor S-nitroso-N-acetyl-penicillamine (SNAP), SNAP + NO scavenger oxyhemoglobin (OxyHb), and SNAP + tissue inhibitor of metalloproteinase material -2 (TIMP-2) respectively. After stimulation, matrix metalloproteinase -13 ( a-MMP-13 ) expression level was detected. Besides, the expression levels of MT1- MMP were detected after SW1353 cells stimulated with SNAP and SNAP + OxyHb, respectively. At last, the activities of the recombinant MMP-13 (r-MMP-13) were detected after r-MMP-13 stimulated with SNAP, recombinant MT1-MMP (r-MT1-MMP) and r-MT1-MMP + TIMP-2,respectively. Results SNAP increased human chondrocytes a-MMP-13 expression, OxyHb and TIMP-2 inhibited a- MMP- 13 expression. SNAP also increased the expression of MT1- MMP levels, OxyHb inhibited the expression of MT1-MMP. r-MT1-MMP activated the r-MMP-13, but SNAP did not, TIMP-2 inhibited the activity of r-MMP-13 induced by r-MT1-MMP. Conclusions NO can not directly activate pro-MMP13 by the role of S-nitroso-(S nitrosylation). MT1-MMP mediates the activiation of pro-MMP-13 induced by NO.

In this paper the last study of the role of nitric oxide(NO) in the development of osteoarthritis is reported. The study of dependability of the content of NO in the inflamed tissue and development of osteoarthritis showed that NO had an important role in the damage of cartilage,and that the inhibition of excess release of NO could improve the repair of cartilage,and that the effective inhibitor of nitric oxide synthase might cure osteoarthritis.

[Objective]To evaluate the preemptive analgesic effect and safety with celecoxib in patients undergone the hip joint replacement.[Method]Fifty patients scheduled for elective hip joint replacement were randomly divided into two groups,celecoxib group and control group.Those of celecoxib group were given celecoxib 200 mg 24,12 h before incision.The operation was performed under extradural anesthesia by the same surgeons. All patients were given celecoxib 200 mg 8,24,36,48,60,72 h after the operation.Before celecoxib administration and after the operation,pain intensity was measured using visual analog scale(VAS),and analgesic requirements,side effects,hip joint ranges of motion,sleep states,hemorheology and phlebothrombosises messured with the ultrasonic wave were compared.[Result]There were no marked differences in the VAS pain scores before celecoxib administration between two groups.Compared with control group,the patients of celecoxib group had significantly lower VAS pain scores after the operation(P

Adult ; Female ; Hemofiltration ; Hemolysis ; drug effects ; Humans ; Male ; Methylprednisolone ; administration & dosage ; therapeutic use ; Middle Aged ; Plasma Exchange ; Treatment Outcome ; Wasp Venoms ; poisoning ; Young Adult

Huaier (Trametes robiniophila) has been widely used as an adjuvant drug for cancer treatment in China. The anti-cancer effect of Huaier extract has been confirmed in liver cancer, lung cancer, breast cancer, ovarian cancer, gastric cancer, and so on. The main mechanisms by which Huaier exerts an anti-neoplastic effect include inhibition of the growth and proliferation of cancer cells, induction of apoptosis of cancer cells, suppression of angiogenesis, inhibition of the invasion and migration of cancer cells, regulation of oncogenes and tumor suppressor genes expression, improving immunity, and reversal of drug resistance in cancer cells. In order to provide references for further study and clinical application on anti-tumor effect of Huaier, the latest research progress on anti-tumor effect of Huaier in recent years is summarized in this paper.
Animals ; Antineoplastic Agents ; pharmacology ; Humans ; Trametes

Objective To investigate effect of alendronate on prevention of aseptic loosening prothesis.Method Twelve beagles which had been implanted a titanium plug in femur were divided into two groups randomly,one received alendronate,and the other placebo as control.After 12 weeks,all beagles were sacrificed.specimens of femur were studied by biomechanical test.Independent sample t test was used for statistical analyses.Results The pull-out strength of the experimental group was higher than that of the control (P ＜ 0.05),and the torsion strength was also higher in the experimental group(P ＜ 0.05).Conclusion The therapy of alendronate can change the bone strength,inhibit aseptic loosening of prosthesis in beagle and could be a good drug for the prevention and treatment of aseptic loosening prothesis.

reliability, content validity, construct validity, and criterion - related validity of the CNS are entirely in accordance with the psychometric demands.

Objective To develop a child neglect scale with Chinese culture background to assess the status of the neglected children in China,and examine the reliability and validity of the child-neglect scale(CNS).Methods Considering the cultural background of China,an item pool was established by revising items in correlative literatures and scales.Then,the first draft of the CNS was improved by reserving the effective items well graded by professional experts.A total of 871 students from 2 junior high schools and a vocational and technical college were involved in the study.Those students were surveyed with Parental Rearing Patterns scale and child neglect scale.Exploratory and confirmatory factor analysis were applied to the development and evaluation of the structure of the scale.Results The findings were as follows:the general Cronbach′s Alpha reliability was 0.85,the split-half reliability was 0.81,the test-retest reliability was 0.90. The CNS was made of the 4 sub-scales which was safe neglect scale,physical neglect scale,communion neglect scale and affection neglect scale.the general Cronbach′s Alpha reliability of the child neglect scale was 0.79-0.85,the split-half reliability was 0.64-0.81,and the test-retest reliability was 0.82-0.90.The item loadings of the neglect scale were over 0.30.The confirmatory factor analysis indicated that the ratio of Chi-square to degrees of freedom were 1.766,the goodness of fit index was 0.917,the Tucker-Lewis index was 0.916,and the root mean square error of approximation was 0.047.Criterion-related validity studies indicated that the scores of the CNS were significantly correlated with the rearing patterns as well(r=0.049,-0.465 P

Adolescent ; Child ; Child, Preschool ; Female ; Gastric Mucosa ; microbiology ; Gastrointestinal Diseases ; diagnosis ; microbiology ; Helicobacter Infections ; diagnosis ; microbiology ; Helicobacter pylori ; growth & development ; isolation & purification ; Humans ; Infant ; Male ; Mouth Mucosa ; microbiology

Cell Line ; Cyclin-Dependent Kinase 5 ; metabolism ; Humans ; Lipopolysaccharides ; Microglia ; cytology ; drug effects ; metabolism ; Stilbenes ; pharmacology