1.Advance in Technique on the Development of Uncultured Microorganism
Xiao-Yuan SUN ; Ying LI ; Ai-Dong QIAN ;
Microbiology 1992;0(05):-
Viable but non-culturable state in bacterial cells as received uncultured microorganism has be- come the fundamental research issues in medical microbiology, epidemiology, general microbial ecology and sanitation quarantine since it was put forward in 1982. When in this state, bacterium are no longer able to grow and form colonies on conventional culture media, but maintain their patheogenicity, may become latent infection escaping detection to threaten enviroment and human security. With the development of modern molecular biology technique and metagenomics, it provide new research method and opportunity for uncul- tured microorganism in the environment. Recently, with the application of the metagenome technique, ge- netic fingerprinting technical etc, it becomes more and more popular. Simultaneously, along with this state become maturity in the laboratory. It provides a new research route for development and exploitation the uncultured microorganism.
2.Effect of intracoronary tirofiban on coronary slow flow patients with acute myocardial infarction during primary percutaneous coronary intervention
Xiang LI ; Chunmei WANG ; Xiaoling ZHU ; Hui AI ; Qian ZHANG
Chinese Journal of Emergency Medicine 2013;(1):69-72
Objective To investigate the effect of intracoronary application of tirofiban on coronary slow flow patients with acute myocardial infarction during primary percutaneous coronary intervention (PPC1).Method It was a retrospective analysis of 187 patients with acute myocardial infarction treated with PPCI in the emergency department of Beijing Anzhen Hospital enrolled in this study from January,2008 through January,2011.The patients divided into 2 groups in terms of intra-coronary administration of tirofiban (tirofiban group) and intra-coronary use of nitroglycerol (control group).Data were statistically analyzed by using SPSS 13.0 software.Categorical variables were analyzed using x2 test and continuous variables were compared by t test.Results Between two groups,there were no differences in preoperative systolic pressure (P =0.245),the rate of TIMI flow 3 (P =0.568) after PPCI and ST segment resolution (P =0.824),LVEF (P =0.275) and in-hospital mortality (P =0.502).Compared with tirofiban group,the systolic pressure was lower and the rate of using intra-aortic counter-pulsation was higher in control group.Although the incidence of slight bleeding in the control group was lower than that in the tirofiban group,no severe bleeding was observed in both groups.Conclusions The effect of intracoronary use of tirofiban was similar to that of nitroglycerol in terms of improving slow flow of coronary artery.It could safely and effectively reduce the incidence of the coronary slow flow in the patients after PPCI,but it produced a little impact on systolic pressure.It may be a better method of choice for AMI patient with low blood pressure.
4.The Establishment of NMR Characteristic Fingerprint of Cordyceps Sinensis and Its Identification Study
Gang CHEN ; Liang HUANG ; Wenjia LI ; Zeping ZHAN ; Libo XIN ; Zhong AI ; Zhengming QIAN ; Guozhu LIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(11):2371-2379
The inundation of Cordyceps sinensis counterfeits in the market makes it difficult to identify. In this study, 21 batches of wild C. sinensis from 3 different regions, 30 batches of naturally cultured C. sinensis and 4 kinds of counterfeits extracted by methanol and water were analyzed using NMR technology. 9 characteristic peaks were defined as quantitative criterion after comparison, and NMR fingerprints of C. sinensis were established. According to the result it is highly similar between naturally cultured C. sinensis and wild ones by comparing their NMR fingerprints. However, NMR spectra of four kinds of adulterants showed differences with C. sinensis. The result also showed that NMR fingerprint of C. sinensis are highly characteristic and specific. The NMR characteristic fingerprint of wild C. sinensis was consistent with the naturally cultured C. sinensis, and it indicated that the chemical constituents of wild C. sinensis and naturally cultured C. sinensis are nearly the same.
6.Clinical Summary of 1260 Patients with "Three Holes and One Hook in The End" Laparoscopic Cholecystectomy
Hai MA ; Wenlai SHEN ; Yang LI ; Xiaohua HU ; Kebai AI ; Dingfeng QIAN
Chinese Journal of Bases and Clinics in General Surgery 2008;0(10):-
Objective To summarize the experiences of "three holes and one hook in the end(TOE)" laparoscopic cholecystectomy(LC) in 1 260 cases and to investigate the operation procedures,technical points and the prevention of complications.Methods The data of 1 260 patients suffering from chronic calculous cholecystitis,acute calculous cholecystitis,atrophic cholecystitis,gallbladder polyps etc.,who were admitted to this hospital and treated by TOE from March 1999 to March 2008 were included and analysed retrospectively in this study.Results One thousand two hundred and sixty of cases were cured,including 1 252 cases of succeeding LC(99.37%),conversions to open in 8 cases,no death,no bile duct injury,with intraoperative hemorrhage in 3 cases,umbilicus infection in 2 cases,gallbladder fossa hydrops in 3 cases,with operation time for 8-60 min(mean 38.5 min) and hospitalization for 3-7 d(mean 5 d) after surgery.During the follow up of 1 002 cases for 1 to 7 years(mean 3.5 years),there were no complications such as bile fistula,bile duct stricture,residual stones of biliary duct,etc..Conclusion TOE is worthy of application and promotion for the excellent effectiveness,few complications,rapid recovery and safety.
7.Evaluation of shear wave elastography in diffuse thyroid disease
Xixi ZHANG ; Qian WANG ; Hong AI ; Min DAN ; Ning LI ; Xiaoping REN ; Wenqian PAN
Chinese Journal of Ultrasonography 2014;23(6):489-492
Objective To explore the value of shear wave elastography (SWE) in diffuse thyroid disease.Methods The elastic modulus were detected by SWE in 41cases of diffuse thyroid disease [including 16 cases of Graves' disease (GD),16 cases of Hashimoto' s thyroiditis (HT) and 9 cases of subacute thyroiditis(SAT)] and 30 cases of healthy volunteers.The elastic modulus,including Emean,Emin and Emax,were measured and compared.Results Compared with the normal group[Emean(15.7-± 2.5)kPa,Emin(11.6 ± 2.4)kPa and Emax (20.2 ± 3.0)kPa],the Emean[(20.4 ± 4.7)kPa],Emin[(14.4-± 3.8)kPa] and Emax [(27.8 ± 7.3)kPa] of GD,the Emean [(18.4-± 5.0)kPa] and Emax [(25.2 ± 5.8)kPa] of HT,and the Emean[(11.0 ± 2.9)kPa] and Emin [(6.0 ± 2.7)kPa] of the SAT were different significantly(P =0.001,0.007,0.001 ; P =0.045,0.001 ; P =0.000,0.000).There were significant differences between the SAT and the other two groups,namely GD and HT (P <0.05).Such differences,however,were not found between GD and HT (P >0.05).Conclusions SWE can be used to measure the elastic modulus of the thyroid tissue quantitatively and objectively,serving as a useful technique to predict the diffuse thyroid disease.
8.Quantitative evaluation in the stage of chronic nephrosis by shear-wave elasticity technology
Qian WANG ; Hong AI ; Xixi ZHANG ; Min DAN ; Ning LI ; Wenqian PAN ; Xiaoping REN
Chinese Journal of Ultrasonography 2014;23(5):414-418
Objective To investigate the diagnostic value of the shear-wave elasticity (SWE) imaging technology on the quantitative diagnosis of chronic nephrosis stage.Methods Sixty patients with nephrosis (nephrosis group) were evaluated with SWE and the renal function test.The Young's modulus value and the renal function were measured,and the results were compared with those of twenty healthy subjects (control group).Results Twenty cases of healthy control group were definited as R0.Sixty patients of nephrosis group were divided into four groups according to renal function:R1-R4.The Young's modulus of the nephrosis group was significantly higher than the control group (P <0.01).There were also statistically significant differences among each stage of the nephrosis group (except R1 and R2 of nephrosis group)(P < 0.01).According the ROC curve,the cut-off value of the Young's modulus was 5.53 kPa when maximum area under the curve equal to 0.886,the sensitivity and specificity were 81.70% and 80.40%.The Young's modulus value and renal function were positively correlated with the stage of nephrosis.The areas under the ROC curves for the Young's modulus,urea nitrogen and csytatin C were 0.965,0.950,0.965 for ≥R3,0.978,0.912,0.961 for =R4,respectively.Conclusions SWE imaging technology provided a new quantitative index for the stage of nephrosis through quantizing the elasticity of the tissue.
10.Analysis on occupational noise deafness in a provincial occupational disease diagnosis institution from 2016 to 2020
YU Xin yang HU Shi jie ZHENG Qian ling YANG Ai chu LI Bin
China Occupational Medicine 2022;49(05):558-
Objective - ( )
To analyze the epidemiological characteristics of occupational noise induced deafness ONID
( )
diagnosed by Guangdong Province Hospital of Occupational Disease Prevention and Control GDHOD from 2016 to 2020 and
- Methods
the reasons non ONID diagnosis. The data of ONID patients diagnosed in GDHOD from 2016 to 2020 were collected
“
from the Occupational Disease Report Card in the Occupational Disease and Occupational Health Information Monitoring
” “ ” -
System subsystem of the China Disease Prevention and Control Information System . The data of non ONID subjects were
,
collected from the occupational disease diagnosis archives in the same hospital and the relevant data were analyzed
Results , , ( )
retrospectively. Of the 1 432 subjects 824 subjects were diagnosed as ONID patients mainly of mild ONID 86.0% .
(M) , M
Male patients accounted for 88.0%. The median of diagnosis age was 45.0 years old and of length of employment of
, , ,
diagnosis was 8.3 years. ONID patients were mainly found in Zhongshan Dongguan Zhuhai Jiangmen and Guangzhou City in
, , ( )
the Pearl River Delta accounting for 67.6%. The cases distributed in 519 enterprises mainly on manufacturing 90.2% .
, - , ;
Among the 139 enterprises each enterprise had 2 11 patients worked within five years accounting for 53.9% 91.1% of the
-, - - -
ONID patients were distributed in large medium and small enterprises. ONID patients mainly worked in non public
enterprises that accounted for 91.3%. There were 606 subjects could not be diagnosed as ONID. The main reasons for not being
( ),
diagnosed were that the weighted value of better ear hearing threshold was less than 26 dB 34.8% the working history of
( ),
occupational noise exposure was less than three years 31.5% the weighted value of better ear hearing threshold was less thanConclusion
26 dB and the average hearing threshold of binaural high frequency was less than 40 dB 16.2% . The ONID
, , -, -
patients have the characteristics of group aggregation. The Pearl River Delta manufacturing industry large medium and
- - :
small non public enterprises are the key points of ONID prevention. The main reasons for not being diagnosed as ONID were
,
the working history of occupational noise exposure was less than three years the weighted value of better ear hearing threshold
, -
was less than 26 dB and the average high frequency hearing threshold of both ears was less than 40 dB.