Objective To observe the changes of cerebral ultrastructure,and cerebral oxidative damage and inflammation reaction in rats after orthotopic liver autotransplantation. Methods Twenty-four male SD rats were randomly divided into control group (Group C,n=8),6-h-reperfusion group (Group M1,n=8),and 24-h-reperfusion group (Group M2,n=8).Rats in the Group C received sham operation after anesthesia,involving laparotomy and vascular separation; rats in the Group M1 and M2 received orthotopic liver autotransplantation, and their brain tissues were obtained 6 and 24 h after reperfusion.The changes of cerebral morphology were observed by transmission electron microscope; the levels of interleukin-6 (IL-6),IL-10 and tumor necrosis factor-a (TNF-a) were measured; the levels of superoxide dismutase (SOD),malondialdehyde (MDA) and catalase (CAT) were observed. Results As compared with those in Group C,the cerebral ultrastructure of Group M1 and Group M2 showed obvious traumatic changes,including microvascular edema and deformation,neuron edema,nuclear deformation with indentation in the surface,increment of heterochromatin in the nuclear,blurring in the karyolemma and perinuclear space, disappearance of reticulate structure in the nucleolus,mitochondria edema and disappearance ofendoplasmic reticulum; and these changes in Group M2 were more obvious than those in Group M2.The changes of oxidative damage and inflammatory responses were obvious:as compared with that in Group C, the SOD level in M2 decreased significantly (P＜0.05), the CAT level in M1 decreased significantly (P＜0.05),and the levels of MDA,IL-6,IL-10 and TNF-a in Group M1 increased significantly (P＜0.05). Conclusion After orthotopic liver transplantation,the pathology changes of cerebral tissues in these SD rats were significant, with obvious oxidative damage and inflammatory reaction.

The Purpose of this study was to obtain data needed for nursing informatics education through analysis of experiences about application of WBI Nursing Informatics Education Program for Graduate Students which was developed by software engineering and nursing educators. This study was conducted on WBI Graduate Nursing Informatics Education Program which was developed for nursing informatics course, dept. of nursing, C university. The WBI Graduate Nursing Informatics Education Program was developed and operated after taking consideration into the level of computer skills and needs about 3 credits nursing informatics course of 28 graduate nursing students. It was identified that most of graduate nursing students were satisfied with WBI Nursing Informatics Education Program, especially system features of this program that were consisted of system approach and interface design. In conclusion, it is considered that CAUNMCC-WBI System is useful teaching-learning program for graduate nursing informatics course. And also it is suggested that this WBI graduate nursing informatics course provided by interdisciplinary faculties could be a alternative for graduate nursing informatics education.
Education* ; Humans ; Nursing Informatics* ; Nursing* ; Students, Nursing

OBJECTIVETo understand the current status and its changing patterns of perinatal health care in some southern and northern areas of China from 1994 to 2000.

METHODSData was collected in 13 counties/cities covered by Perinatal Health Care Surveillance System which was part of the Sino-American cooperative project on neural tube defects prevention, established in 1992. The study population consisted of 368 589 women who delivered single live births of at least 20 gestational weeks from 1994 through 2000. Chi-square test was employed to test the difference on the proportions of women with prenatal checkup, women with early prenatal care onset, women with at least five prenatal visits, hospital delivery versus home delivery as well as the proportion of women with at least three postpartum visits. Two-way ANOVA was used to test the differences of mean of gestational weeks at first prenatal visit and the mean of prenatal visits between different year and areas.

RESULTSThe proportion of women with prenatal checkup remained over 99.0% from 1994 through 2000. The mean of gestational weeks at first prenatal visit changed from 13.1 weeks in 1994 to 10.7 weeks in 2000. The proportions of women with early prenatal care onset increased from 65.5% in 1994 to 79.4% in 2000 while women with at least five prenatal visit increasing from 34.1% to 71.8%, hospital delivery increasing from 91.3% to 98.8%, home delivery decreasing from 5.6 % to 0. 6%, women with at least three postpartum visits increasing from 80.5 % to 95.1%. Except for the proportion of women with early prenatal care onset, other proportions in northern areas were lower than those in southern areas in the same year. Greater differences between urban and rural areas in northern areas were found than in southern areas.

CONCLUSIONThe utilization of perinatal health care services was greatly improved in both southern and northern areas from 1994 to 2000. However, there were still obvious differences in the perinatal health care services between northern and southern areas as well as between urban and rural areas in northern China.

China ; Female ; Health Surveys ; Humans ; Perinatal Care ; utilization ; Pregnancy ; Rural Health ; Urban Health

Objective Our purpose was to evaluate the association between hemoglobin concentration (Hb) and cognitive ability of children at 4-6 years of age in 21 counties/cities in China.Methods A total number of 7331 children born during 1993-1996 were randomly selected from 21 counties or cities in Hebei,Jiangsu and Zbejiang provinces,Hb concentration of children were measured followed by three tests including full-scale,verbal and performance intelligence quotient (IQ) test performed by Chinese-Wechsler Intelligence Scale for Children,one year later.Results There were a 0.7 point difference in mean verbal scale IQ(P=0.144) and a 0.9 point difference in both mean performance and full-scale IQ (P=0.055 and 0.079,respectively) between anemia and non-anemia groups.Compared with children with non-anemia,children with anemia were 1.3-fold more likely to score poorly in verbal IQ and operational IQ (95%CI:1.1-1.6,1.1-1.5,respectively) and 1 A-fold more likely to have had poor scores in full-scale IQ(95%CI:1.2-1.6) after controlled for children's gender,age at intelligence test,region,parity and mother's IQ,education level,occupation.Participants were divided into 5 sub-groups according to Hb concentration of every 20 percentile.Verbal IQ scores of the lowest(Hb＜110 g/L),moderate (117 g/L≤Hb＜122 g/L) and the highest Hb concentration groups (Hb≥130 g/L) were 90.6±18.1,94.0±17.6 and 91.0±16.4,respectively.Performance IQ scores were 102.2±15.7,104.6±14.8 and 100.5±14.9,respectively.Full-scale IQ scores were 95.94±17.3,99.0±16.4 and 95.2±15.6,respectively.Children with both low and high hemoglobin levels did poorly in all intelligence tests than children with moderate Hb concentration (P＜0.001).After controlling for confounding factors,children with the lowest concentration were 1.4-fold more likely to have had poor verbal and performance scores than children with moderate Hb concentration (95% CI:1.1-1.7,1.1-1.8,respectively) and 1.5-fold (95% CI:1.2-1.8) more likely to have had poor full-scale scores than those with moderate Hb concentration.The association between high Hb concentration and low IQ scores disappeared in the multivariate model.Conclusion Low Hb concentration might have adversely affected children' s cognitive development.

Objective To analyze the influencing factors for stunting and underweight among children aged 3-6 years in 15 counties of Jiangsu and Zhejiang provinces so as to provide reference for enhancing growth level among preschool children. Methods Data was from the 183 295 records of Children Follow-up Study Project carried out by the Institute of Reproductive and Child Heath of Peking University and the records of related perinatal health care surveillance system in rural areas of 15 counties/cities of Jiangsu and Zhejiang provinces. WHO-NCHS standard was used to assess the childhood physical level of growth. Data of children's birth and their mothers' perinatal health were correlated to determine influencing factors for childhood stunting and underweight. Results The average stunting rate was 7.95% and underweight rate was 1.55%. Sex, birth weight, preterm birth as well as maternal height, maternal BMI at the first prenatal visit, maternal education and occupation were significantly related to childhood stunting and underweight. Birth weight was the most important influencing factor for childhood underweight. For the groups whose birth weights were ＜2500 g and ≥2500 g, the rates of underweight were 7.77% and 1.46% respectively. Children with low birth weight were at higher risk for underweight (OR=3.68,95% CI: 3.11-4.37). Maternal height was the most important influencing factor for childhood stunting. For the groups whose mothers' heights were ＜155 cm, 155-160 cm, 160-165 cm and ≥165 cm, the stunting rates were 13.01%, 8.76%,6.21% and 4.14% respectively. Compared with the ≥165 cm group, the ＜155 cm group was at higher risk for stunting (OR=3.08, 95% CI: 2.82-3.37). Conclusion Birth weight and maternal height were key factors influencing the growth of children. Perinatal health care and the nutrition starus of pregnant mothers should be improved to promote the growth level of preschool children.

Objective The purpose of this study was to evaluate the effect of hemoglobin (Hb) level during early gestation on the cognitive development of children at 4-6 years of age.Methods A total number of 3609 children were randomly selected from all the live birth infants whose mothers participated in a community intervention trial during 1993-1996 in 13 counties or cities in Jiangsu and Zhejiang provinces. Hb concentration during early gestation was measured at first prenatal examination and intelligence quotients (IQ), including full-scale, verbal and performance were assessed using Chinese-Wechsler Intelligence Scale for Children in 2000-2001 when these children had a mean age of 68 months. Results Compared with children whose mothers were non-anemic during early gestation, children whose mothers were anemic had a 0.6 point higher mean verbal scale IQ, a 0.9 point higher mean performance IQ and a 0.8 point higher mean full-scale IQ.These differences were not statistically significant when children' s gender, age at intelligence test,region, parity and mother' s IQ, education level and occupation were adjusted for. When mother-child pairs were divided into 5 sub-groups of every 20 percentiles according to Hb concentration during early gestation, verbal IQ scores of the lowest(Hb＜ 103 g/L), the moderate(110 g/L≤ Hb＜ 116 g/L)and the highest Hb concentration group (Hb≥124 g/L) were 91.6 ± 18.9, 92.8 ± 18.2 and 90.3 ±18.6, respectively. The performance IQ scores were 104.7 ± 15.2, 1 04.5 ± 14.3 and 103.5 ±15.1, and full-scale IQ scores were 97.8 ± 17.3,98.4 ± 16.3 and 96.4 ± 17.4, respectively. After controlling for confounding factors, children whose mothers had highest Hb concentration were 54% (OR= 1.54,95%CI:1.13-2.11)more likely to have poor verbal scores and 53% (OR= 1.53,95%CI: 1.10-2.12)more likely to have poor full-scale scores than children whose mothers had moderate -Hb concentration. No statistical associations were noticed between high -Hb concentration and performance scores, or between low Hb concentration during early gestation and verbal, performance as well as full-scale score of pre-school children. Conclusion High maternal Hb concentration during early gestation might adversely affect children' s cognitive development.

OBJECTIVETo develop the first grade standard analytical reference material of bovine blood lead.

METHODSAccording to standards and technology specification of primary standard reference material (JJG1006-1994), ISO 17511, and volume-effect relationship, a bovine blood lead model was developed by adding acetate lead in the feed in dose of 2-5 mg x kg(-1) x d(-1). Cow blood was collected when blood lead concentration went up to low, medium and high concentration range (90-100, 190-200, 280-300 microg/L). Blood sample was kept in tube and frozen after irradiation. The samples were tested for homogeneity and stability. ID-MS method was used to measure the lead concentration through comparison with two standard lead samples from the USA NIST SRM 955b.

RESULTSSamples of the three lead concentrations showed uniformity by single factor analysis of variance (F = 0.61, 1.64, 0.28, respectively, P > 0.05) . After 14 months monitoring, the RSD was 0.85%, 1.05% and 0.49% (t = 0.787, 1.132, 0.854, respectively, P > 0.05). The characteristic and indefinite values were 102.4 +/- 5.5; 181.2 +/- 4.0; 304.5 +/- 3.9, respectively (unit: microg/L). The reproduction of the two USA NIST SRM 955b samples further showed our methods and procedures were correct. Our sample was stabile for four days at room temperature.

CONCLUSIONThe standard reference material of bovine in our research had reached the national standard material requirements.

Animal Feed ; Animals ; Blood Chemical Analysis ; standards ; Cattle ; Lead ; blood ; Male ; Models, Animal ; Reference Standards

Objective To examine the association between pregnancy-induced hypertension(PIH) and small-for-gestational-age babies(SGA)in a Chinese population.Methods Subjects were women who delivered a singleton baby(gestational weeks:equal to or greater than 28,and less than 42)in four cities or counties in Jiangsu and Zhejiang provinces,China,during the period of 1995-2000.A total number of 93 743 women were included.Incidence of SGA was calculated and compared between women with or without PIH and between groups with different severities of PIH.Multiple logistic regression was used to address the relationship between PIH and SGA while controlling for maternal age,occupation,education,parity,BMI,anemia,premature rupture of membranes and fetal sex.The association between PIH and SGA was also examined according to preterm or term delivery.Results The incidence of SGA in women with PIH(6.0%)was higher than women without(4.5%),and the incidence increased with severities of PIH.The adjusted relative risk rates(95%CI)of SGA in women with mild,moderate and severe PIH were 1.17(1.01-1.34),1.69(1.33-2.14),and 3.50(2.57-4.77),respectively,when confounders were controlled for.The risk ratios of SGA in women with PIH among women who delivered a preterm baby wete higher than those among women who delivered a term baby.Conclusion There seemed a statistical association between PIH and SGA,and women with PIH having higher incidence of SGA than those withont PIH.

OBJECTIVETo investigate the association between third trimester hemoglobin (Hb) concentrations and the risk of low birth weight and preterm delivery in a Chinese population.

METHODSSubjects were women who delivered in four cities/counties in Jiangsu and Zhejiang provinces, China, during the period of 1995 - 2000. Incidence of low birth weight and preterm delivery was calculated and compared among groups of women with different levels of Hb during the third trimester. Multiple logistic regression was used to address relationships between Hb levels and the risk of preterm delivery and low birth weight while controlling for potential confounding factors.

RESULTSThe overall prevalence of anemia during third trimester of pregnancy was 48.2% , mainly consisting of mild and moderate anemia. Mild and moderate anemia did not increase the risk of preterm delivery and low birth weight statistically. The lowest incidence of preterm delivery and low birth weight was found among pregnant women with Hb levels at 90-99 g/L. The risk for preterm delivery and low birth weight increased with either increasing or decreasing hemoglobin concentrations. However,there was no remarkable elevation of the risk when Hb was in the range of 70-119 g/L. Women with severe anemia (Hb< 70 g/L) had 80% higher risk (95% CI:1.0-3.3) of preterm delivery and a 4.0-fold higher risk (95 % CI :2. 1-7.5) of low birth weight compared with women with an Hb value of 90-99 g/L. In addition, women with a high Hb concentration (Hb> 130 g/L) had 20% higher risk (95 % CI: 1..0-1.4) of preterm delivery and 50 % higher risk (95 % CI: 1.2-1.9) of low birth weight.

CONCLUSIONA U-shape relationship was found between Hb concentration and the risk of preterm delivery and low birth weight. Severe anemia and high hemoglobin concentration were both associated with increased risk of preterm deliveries and low birth weight.

Adult ; Delivery, Obstetric ; Female ; Hemoglobins ; metabolism ; Humans ; Infant, Low Birth Weight ; Infant, Newborn ; Infant, Premature ; Pregnancy ; Pregnancy Trimester, Third ; Premature Birth ; blood ; epidemiology ; Risk Factors ; Young Adult

Objective: To explore the safety and effectiveness of stereotactic body radiation therapy (SBRT) for oligometastases from colorectal cancer (CRC). Methods: This is a prospective, single-arm phase Ⅱ trial. Patients who had histologically proven CRC, 1 to 5 detectable liver or lung metastatic lesions with maximum diameter of any metastases ≤5 cm were eligible. SBRT was delivered to all lesions. The primary endpoint was 3-year local control (LC). The secondary endpoints were treatment-related acute toxicities of grade 3 and above, 1-year and 3-year overall survival (OS) and progression free survival (PFS). Survival analysis was performed using the Kaplan-Meier method and Log rank test. Results: Petients from 2016 to 2019 who were treated in Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College. Forty-eight patients with 60 lesions were enrolled, including 37 liver lesions and 23 lung lesions. Forty-six patients had 1 or 2 lesions, with median diameter of 1.3 cm, the median biologically effective dose (BED(10)) was 100.0 Gy. The median follow-up was 19.5 months for all lesions. Twenty-five lesions developed local failure, the median local progression free survival was 15 months. The 1-year LC, OS and PFS was 70.2% (95% CI, 63.7%~76.7%), 89.0% (95% CI, 84.3%~93.7%) and 40.4% (95%CI, 33.0%~47.8%). The univariate analysis revealed that planning target volume (PTV) and total dose were independent prognostic factors of LC (P<0.05). For liver and lung lesions, the 1-year LC, OS and PFS was 58.7% and 89.4% (P=0.015), 89.3% and 86.5% (P=0.732), 30.5% and 65.6% (P=0.024), respectively. No patients developed acute toxicity of grade 3 and above. Conclusion: SBRT is safe and effective treatment method for oligometastases from CRC under precise respiratory motion management and robust quality assurance.
Colorectal Neoplasms ; Humans ; Liver/pathology* ; Lung/pathology* ; Prospective Studies ; Radiosurgery/methods*