2.Full-factor design of experiment application in the development of printing ink for binder jetting 3D printed preparation
Zeng-ming WANG ; Xian-fu LI ; Xiao-lu HAN ; Ai-ai LIU ; Xiao-xuan HONG ; Cong-hui LI ; Ai-ping ZHENG
Acta Pharmaceutica Sinica 2021;56(5):1460-1469
The development of printing ink is a challenge for binder jetting 3D printed preparations, which directly determines the quality of the printed product. This study adopted a 23 full-factor Design of Experiment (DoE) with three central points to optimize the printing ink composition of levetiracetam 3D printed dispersible tablet based on the concept of Quality by Design. Firstly, using polyvinyl pyrrolidone K30, glycerin and polysorbate 20 as independent variables based on 40% (
3.Expressions and significance of Survivin and VEGF in hepatocellular carcinoma
Kai-Fu KANG ; Ai-Wen CHE ; Xiao-Wu CHEN ; Xiang-Cheng SHI ; Jian-Ping CHEN ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(10):-
Objective To explore the expressions of Survivin and VEGF and relationship between them in hepatocellular carcinoma(HCC).Methods The expressions of Survivin protein and VEGF protein in 50 HCC.30 cirrhosis and 10 normal tissues were assessed by immunohistochemical method.The expressions of Survivin mRNA and VEGF mRNA in 50 HCC,30 cirrhosis and 10 normal tissues were assessed by in situ hybridization.Results The expressions of Survivin and VEGF in cancer tissues,cirrhosis tissues,normal tissues weresignificantly different. The expression of Survivin in HCC tissues was stronger than that in cirrhosis,but the expreesion of VEGF in cirrho- sis was stronger than that in HCC tissues.Conclusion The expression of survivin.is closely associated with the ex- pression of VEGF in HCC and they take positive correlation.The abnormal expressions of Survivin and VEGF are closely associated with the development of HCC.They may play important roles in the development of HCC.
5.Two methods of hepatic vascular exclusion for hepatectomy
Wei-Ping ZHOU ; Ai-Jun LI ; Si-Yuan FU ; Ze-Ya PAN ; Yuan YANG ; Liang TANG ; Meng-Ehao WU
Chinese Journal of General Surgery 2000;0(12):-
Objective To compare the effects of hepatic vein occlusion with tourniquet and Satinsky clamp in reseeting liver tumor involving the second hepatic portal.Methods From Jan 2003 to Jun 2006,180 patients underwent major liver resection with the selective hepatic vascular exclusion (SHVE).According to methods of hepatic vein occlusion,they were divided into two groups:Occlusion with tourniquet(tourniquet group,n=95)and occlusion with Satinsky clamp(Satinsky clamp group,n= 85).In tourniquet group,the hepatic veins were encircled and occluded with tourniquet,and in Satinsky clamp group,the hepatic veins were not encircled and clamped directly with Satinsky clamp.Data regarding the intraoperative and postoperative courses of the patients were analyzed.Results There was no difference between the two groups regarding the operating time,ischemia time,intraoperative blood loss and postoperative complications rate.The dissecting time of hepatic veins was significantly shorter in Satinsky group(6.2?2.4 min vs 18.3?6.2 min).lu the tourniquet group,five hepatic veins(one fight hepatic vein and four common trunk of left-middle hepatic veins)could not be dissected and encircled because of the tumors involving the cava hepatic junction.Another patient's common trunk of left-middle hepatic vein was inadvertently lacerated during the dissection.Hepatic veins in these 6 patients were occluded with Satinsky clamp successfully.Conclusion Occlusion with Satinsky clamping is safer and easier procedure than tourniquets in the resection of liver tumor involving the second porta hepatis.
6.Application and prospect of alkyl polyglycosides absorption enhancers in drug delivery system
Xian-fu LI ; Zhi-wei ZHANG ; Xiao-xuan HONG ; Xiao-lu HAN ; Meng LI ; Zeng-ming WANG ; Ai-ping ZHENG
Acta Pharmaceutica Sinica 2021;56(6):1591-1598
The efficient and safe delivery of drugs to the therapeutic site through the biofilm has traditionally been a difficult and hot topic in the field of drug delivery. In recent years, alkyl polyglycoside (APG) have become ideal penetration enhancers for drug delivery systems because of their high permeability, good safety and biodegradability, which has attracted wide attention of domestic and foreign researchers. In this paper, the physical and chemical properties, characteristics, action mechanism and application of APG in drug delivery system are reviewed, and its application prospect in drug delivery system is prospected.
7.Specific antitumor activity and mechanism of protonic bis-phenanthroline
Zi-zhen ZHAO ; Chen FU ; Zhi-hong CUI ; Xiao-rong LI ; Ying-ying ZHANG ; Yu-ping ZHANG ; Xiao-xi YANG ; Ai-ling FU
Acta Pharmaceutica Sinica 2022;57(5):1344-1351
Hepatocellular carcinoma (HCC) is a common malignant tumor worldwise. The incidence rate of HCC is high and is easy to metastasis and recurrence, which seriously affects human health. Traditional chemical drugs have some challenges such as toxicity, side effects, and multidrug resistance, thus it is urgent to find new drugs and effective targets. Here we synthesized a novel chemical, protonic bis-phenanthroline (H-BP), and the antitumor effect was investigated in the study. The results showed that H-BP could selectively inhibit the proliferation of tumor cells and cause HCC apoptosis. And also, in HCC tumor-bearing mice, H-BP could effectively prevent the growth of tumor mass, even completely eliminate the tumor at medium dose (5 mg·kg-1) and high dose (10 mg·kg-1), and meanwhile H-BP has no significant effect on the body weight of mice. The experimental protocol was approved by the Animal Ethics Committee of Southwest University, and the experimental operation was strictly carried out in accordance with the ethical principles of animal use and care. Mechanism studies showed that H-BP induced HCC apoptosis was related to down-regulation the expression of pleomorphic adenoma gene like-2 (PLAGL2), a oncogene transcription factor, resulting in the down-regulation of PLAGL2 downstream proteins hypoxia inducible factor and
8.UPLC/Q-TOF MS and NMR plant metabolomics approach in studying the effect of growth year on the quality of Polygala tenuifolia.
Ying XUE ; Xiao-wei LI ; Zhen-yu LI ; Zu-ping ZENG ; Fu-sheng ZHANG ; Ai-ping LI ; Xue-mei QIN ; Bing PENG
Acta Pharmaceutica Sinica 2015;50(3):340-347
Growth year is one of the important factors for the quality of Polygala tenufolia. In this study, primary metabolites and secondary metabolites were compared in 1, 2 and 3 years old P. tenufolia cultivated in Shaanxi Heyang. The samples were subjected to ultra-high performance liquid chromatography (UPLC)-quadrupole time-of-flight mass spectrometry (Q-TOF MS) and nuclear magnetic resonance (NMR) analysis, and the obtained data were analyzed using principal component analysis (PCA) and other statistical analysis methods. In addition, content and correlation of different metabolites were also calculated. The results showed no significance between main component contents in 2 year-old and 3 year-old P. Tenufolia, but 1 year-old was statistically different. The contents of primary metabolites, such as fructose, sucrose, and choline increased as time goes on, while glycine and raffinose decreased. The contents of secondary metabolites, such as onjisaponin Fg, polygalasaponin XXVIII, polygalasaponin XXXII increased, while polygalaxanthone III and parts of oligosaccharide multi-ester including tenuifoliose A, tenuifoliose C, tenuifoliose C2 and tenuifoliose H decreased with the extension of the growth years. Growth years has important impact on the quality of P. tenuifolia and the existing growing years of commodity P. tenuifolia have its scientific evidence. This study supplied a new method for the quality evaluation of Chinese medicinal materials.
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Metabolomics
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Oligosaccharides
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Plants, Medicinal
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chemistry
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Polygala
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chemistry
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Quality Control
9.Dynamic distribution of L. interrogans in guinea pigs and pathologic changes in experimental leptospirosis.
Hong-liang YANG ; Xu-cheng JIANG ; Ping ZHU ; Wen-jun LI ; Ai-fen FU ; Ling-zi ZHAO ; Xiao-kui GUO ; Guo-ping ZHAO
Chinese Journal of Pathology 2005;34(9):597-598
Animals
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Female
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Guinea Pigs
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Kidney
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microbiology
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pathology
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Leptospira interrogans
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isolation & purification
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pathogenicity
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Leptospirosis
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microbiology
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pathology
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Liver
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microbiology
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pathology
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Lung
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microbiology
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pathology
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Male
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Time Factors
10.The application of the PET/CT technology in the diagnosis of atypical spinal tuberculosis
Ai-Ping CHENG ; Li-Ping FU ; Mei-Ling SUN ; Jian-Lan FU ; Xiao-Gang WANG ; Fa-Huan SONG
Journal of Preventive Medicine 2017;29(6):545-549
Objective To explore the diagnostic value of PET/CT technique in the atypical spinal tuberculosis. Methods A total of 60 patients who had suffered from spinal tuberculosis and spinal metastasis confirmed by surgical interventions from 2010 to 2016 were retrospectively analyzed. 18F-FDG PET/CT examination was made at our department. Image data of X-ray, CT, and PET/CT were compared in the diagnosis accuracy. Results A total of 52 males and 8 females, aged from 38 to 82 years (mean, 50.2 years) were collected. The statistical analysis showed PET/CT technology with sensitivity of 85%, and specificity of 95%, which were significantly higher than those of X-ray and CT (AUC=0.900, P<0.05) . 18F-FDG PET/CT can find more lesions than X-ray and CT, find "cold abscess" and "cold zone" of spinal tuberculosis, that was an exclusive value of PET/CT diagnosis for atypical spinal tuberculosis. Conclusion 18F-FDG PET/CT shows an important clinical diagnostic value for the atypical spinal tuberculosis.