Adult ; Aged ; Aged, 80 and over ; Antifungal Agents ; therapeutic use ; Female ; Hematologic Neoplasms ; drug therapy ; microbiology ; Humans ; Male ; Middle Aged ; Mycoses ; complications ; drug therapy ; Pyrimidines ; therapeutic use ; Treatment Outcome ; Triazoles ; therapeutic use ; Voriconazole ; Young Adult

The rate of corneal graft rejection is still high for high-risk keratoplasty although immune suppression drug is routinely used.The role of traditional Chinese medicine in corneal transplantation is concerned gradually.Heijingpaichitang on the prevention and treatment of rats with high-risk corneal allograft rejection needs further study.Objective This study was to investigate the inhibitory effect of heijingpaichitang on high-risk corneal transplantation immune rejection in rats.Methods Sixteen female SD rats were used as the donors and 32female Wistar rats were served as recipients.The high-risk corneal trasplantation models were established by corneal suture in 32 Wistar rats,and then homogeneity variant SD-Wistar corneal transplantation was performed.The recipients were randomized into model control group,cyclosporinc A (CsA)group,heijingpaichitang group and CsA +heijingpaichitang group.CsA,heijingpaichitang and CsA + heijingpaichitang was orally administered 4 days after operation once per day for 15 days,and normal saline solution was used at the same way in the model control group.Ocular anterior segment reaction was examined under the slit lamp and corneal opacification,edema and neovasculation were scored based on Larkin' s criteria.Rejection index of the corneal graft was recorded and the graft survival time was calculated.The pathological examination of the corneal graft was carried out in all rats,and the inflammatory cells in the corneas and CD4+ cells in the periphery blood were assayed using flow cytometry.The use of the animals complied with ARVO Statement.Results Corneal graft rejection occurred in 10 days after operation in the model control group,12-13 days in the CsA group and heijingpaichitang group and 22 days in the CsA +heijingpaichitang group.Compared with model control group,the scores of the corneal opacification,corneal edema and neovascularization were significantly lower in the CsA group,heijingpaichitang group and CsA+heijingpaichitang group (P＜0.05),and all the scores were declined in the CsA+ heijingpaichitang group compared with CsA group and heijingpaichitang group(P＜0.01),but no significant differences were seen in the scores between the CsA group and heijingpaichitang group(P＞0.05).The mean survival time of grafts was (10.38 ±1.69)days in the model control group,(22.50 ± 3.07) days in the CsA + heijingpaichitang group,with the significant difference (t =-9.790,P =0.000).The pathological examination of graft showed that the lymphocytes and new blood vessels were less in the CsA+heijingpaichitang group compared with CsA group and heijingpaichitang group 15 days after operation.Flow cytometry verified that the number of lymphocytes in graft,CD4+cells and CD4+/CD8+ in periphery blood were significantly lower in the heijingpaichitang group,CsA group and CsA+heijingpaichitang group compared with model control group (P＜0.05).Conclusions Heijingpaichitang can inhibit immune rejection to certain extent in high-risk corneal transplantation rat and has a similar effect to 0.1％ CsA.Heijingpaichitang and 0.1％ CsA have a synergistic effect.

Objective To evaluate the feasibility of reconstructing horizontal periodontal bone defects by tissue engineering based on bone marrow stromal cells(BMSCs)as seed cells and enamel matrix proteins(EMPs)as growth factors. Methods Two healthy rhesus monkeys were selected, and BMSCs were isolated from iliac marrow and serial subcultivation was conducted. The cells of induced BMSCs at passage 3 were harvested and mixed with Bio-oss collagen. The models of horizontal periodontal bone defects were established surgically in each buccal side of the posterior teeth, and were divided into four groups (blank control group, material group, cells/material group and cells/material/EMPs group). The histological and Micro-CT observation were carried out 8 weeks later. Results In the blank control group, the defects were filled with fibrous connective tissue. There was newly-formed alveolar bone in the material group. In the cells/material group, periodontal regeneration could be observed, while the newly-formed cementum was irregular and less in quantity. In the cells/material/EMPs group, the amount of newly-formed alveolar bone was larger, and the newly-formed cementum was continuous and regular. Conclusion The tissue engineering technique of BMSCs as seed cells in combination with EMPs induction can significantly promote the regeneration of periodontal tissue.

Objective To evaluate the clinical efficacy and safety of tacrolimus capsules combined with prednisolone acetate tablets in the treatment of systemic lupus erythematosus.Methods A total of 54 patients with systemic lupus erythematosus were randomly divided into control group and treatment group with 27 cases per group.Control group was treated with prednisolone acetate tablets 40 mg qd,oral.Treatment group was given tacrolimus capsules 1 mg per time bid,oral,on the basis of control group.The clinical efficacy,interleukin-4 (IL-4),monocyte chemoattractant protein-4 (MCP-4),white blood cell (WBC),platelet (PLT),erythrocyte sedimentation rate (ESR) and adVerse drug reactions were compared between two groups.Results After treatment,the total effective rates in treatment and control groups were 92.59％ (25/27 cases) and 70.37％ (19/27 cases) with significant difference (P ＜ 0.05).After treatment,the main indexes in treatment and control groups were compared:IL-4 were (135.67 ± 16.88),(165.47 ±23.22)ng · L-1;MCP-4 were (3.17 ± 0.46),(4.98 ±0.71)ng· L-1;WBC were (4.83 ±0.58) ×109,(3.67 ±0.46) × 109/L;PLT were (153.22±18.85) ×109,(113.22 ± 16.26) × 109/L;ESR were (15.26 ± 3.14),(23.65 ± 3.58)mm · h-1,the differences were statistically significant (P ＜ 0.05).The adverse drug reactions in two groups were based on leukopenia,gastrointestinal discomfort and infection,and the incidences of adverse drug reactions in treatment and control groups were 18.52％ and 14.81％,without significant difference (P ＞ 0.05).Conclusion Tacrolimus capsules combined with prednisolone acetate tablets have a definitive clinical efficacy in the treatment of systemic lupus erythematosus,which can significantly reduce the serum levels of IL-4 and MCP-4,improve clinical efficacy,without increasing the incidence of adverse drug reactions.

Background: Despite substantial progress toward measles control are making in China, measles outbreaks in immunocompromised population still pose a challenge to interrupt endemic transmission. This study aimed to investigate the features of measles in pediatric hematology and oncology patients and explore the reasons behind the outbreak. Methods: We collected demographic, epidemiological, and clinical data of immunocompromised measles children.All suspected measles cases were laboratory?confirmed based on the presence of measles IgM and/or identification of measles RNA. The clinical data were statistically analyzed by t?test for continuous variables and Fisher''s exact test for categorical variables. Results: From March 9 to July 25 in 2015, a total of 23 children with malignancies and post hematopoietic stem cell transplantation (post?HSCT) were notified to develop measles in Shanghai. Of these 23 patients with the median age of 5.5 years (range: 11 months–14 years), 20 (87.0%) had received 1–3 doses of measles vaccine previously; all patients had fever with the median fever duration of 8 days;21 (91.3%) had cough; 18 (78.3%) had rash; 13 (56.5%) had Koplik''s spot; 13 (56.5%) had complications including pneumonia and acute liver failure; and five (21.7%) vaccinated patients died from severe pneumonia or acute liver failure. Except the first patient, all patients had hospital visits within 7–21 days before measles onset and 20 patients were likely to be exposed to each other. Conclusions: The outcome of measles outbreak in previously vaccinated oncology and post?HSCT pediatric patients during chemotherapy and immunosuppressant medication was severe. Complete loss of protective immunity induced by measles vaccine during chemotherapy was the potential reason. Improved infection control practice was critical for the prevention of measles in malignancy patients and transplant recipients.

OBJECTIVETo investigate maternal-fetal transmission at human bocavirus (HBoV).

METHODSIgG antibody to HBoV in serum samples of 316 mothers were determined with ELISA and HBoV DNA was determined with real time PCR in the sera of the mothers and their infants.

RESULTSHBoV-IgG was positive in 40.20 percent (127/316) of the mothers, while it was positive in 29.43 percent (93/316) of the cord blood specimens of the infants. The difference between the two groups was significant (X2=8.12, P less than 0.005); 93 samples of both the mothers and the infants were positive for HBoV-IgG.

CONCLUSIONHBoV-IgG can cross the placenta to the fetuses through placenta. Further study is needed to answer the question whether vertical maternal-fetal transmission occurs.

Adult ; Antibodies, Viral ; blood ; Bocavirus ; DNA, Viral ; blood ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin G ; blood ; Infant, Newborn ; Infectious Disease Transmission, Vertical ; Parvoviridae Infections ; transmission ; Pregnancy ; Prospective Studies

OBJECTIVETo analyze the incidence of low birth weight and identify the influencing factors in China in 2006.

METHODSThe national survey was performed by stratified random cluster sampling method in 43 cities and counties in 14 provinces. The subjects were all live neonates during May to October in 2006. Their body weight was measured according to the standardization conducted by the national research team, while the information of all infants was investigated by questionnaires. Non-conditional logistic regression was used to analyze the influencing factors.

RESULTSAmong 18 554 infants investigated, the incidence of low birth weight was 4.6% in 2006 in China, being 3.2%, 4.4%, and 6.3% in eastern middle and western areas respectively. The incidence of low birth weight were 3.9% in urban and 4.8% in rural. The results of logistic regression analysis suggested that maternal education (OR = 1.48; 95% CI: 1.181 approximately 1.861), body mass index (BMI) before pregnancy (OR = 1.52; 95% CI: 1.229 - 1.876), body weight gain in gestation period (OR = 1.57; 95% CI: 1.246 - 1.965), gestational weeks (OR = 20.16; 95% CI: 15.456 - 26.297), multiple birth (OR = 12.11; 95% CI: 9.229 - 15.893), gestation syndrome, and nutrition instruction (OR = 0.66; 95% CI: 0.550 - 0.798) were associated with low birth weight significantly.

CONCLUSIONThe main factors resulting in low birth weight in neonates are maternal education, BMI before pregnancy, body weight gain in gestation period, pregnancy age, multiple birth, gestation syndrome, and nutrition instruction.

Body Mass Index ; China ; epidemiology ; Female ; Humans ; Infant Mortality ; Infant, Low Birth Weight ; Infant, Newborn ; Mothers ; Pregnancy ; Risk Factors ; Surveys and Questionnaires

OBJECTIVETo evaluate the effects of basic fibroblast growth factor(bFGF) on proliferation of periodontal fibroblast-like cells in vivo.

METHODSA U-shaped osseous defect was produced on the buccal side of the mesial root. Four posterior teeth were conducted in four quadrants. Each quadrant included 4 groups: control, bFGF, expanded polytetrafluoroethylene(ePTFE) membrane, bFGF + ePTFE. Each time the 4 teeth sites in one quadrant were operated weekly and each dog experienced 4 times of operations. Bromodeoxyuridine(BrdU) was injected 1 hour prior to sacrificing the dogs at 4 weeks after first surgery. Immunohistochemical method was applied to count the BrdU-labeled fibroblast-like cells.

RESULTSThe number of BrdU-labeled cells reached the maximum at the 2nd week among all groups and then, decreased with time. Both bFGF and bFGF + ePTFE treated group had significantly more BrdU+ cells than remained control or ePTFE groups (P < 0.05) at 1st, 2nd weeks after surgery.

CONCLUSION2 weeks after periodontal surgery is active phase of proliferation of periodontal fibroblasts. bFGF enhances fibroblast proliferation in early periodontal wound healing, and in turn accelerate periodontal regeneration.

Animals ; Cell Division ; drug effects ; Dogs ; Fibroblast Growth Factor 2 ; pharmacology ; Fibroblasts ; cytology ; Periodontium ; cytology ; surgery ; Regeneration ; Wound Healing ; drug effects

OBJECTIVETo investigate the method for creation of auriculocephalic sulcus.

METHODSThe reconstruction was performed 4-12 months after the first surgery. Skin incision was made 5mm posterior to the outer margin of the auricle. The ear framework was elevated with a thick fascia at the deep surface. The costal cartilage banked at the first operation was shaved and transplanted to the deep surface of the concha with sutures. The position and angle of the ear framework was adjusted to be familiar to the healthy ear. The auriculocephalic angle was slightly larger than that in the contralateral ear. Two flaps were designed at the upper and lower area of reconstructed ear and rotated to cover the cartilage. The wound at the donor site was closed with skin graft.

RESULTSA total of 72 patients were treated. All the flaps survived completely. 51 patients were followed up for 3-24 months with satisfactory results. The auriculocephalic sulcus maintained at about 20-30 degree.

CONCLUSIONSIt is a simple, safe and reliable method to create a auriculocephalic sulcus with two random skin flaps from mastoid area combined with skin graft.

Adolescent ; Cartilage ; transplantation ; Dermatologic Surgical Procedures ; methods ; Ear ; Ear Auricle ; surgery ; Ear Deformities, Acquired ; surgery ; Fascia ; Humans ; Mastoid ; Ribs ; Skin Transplantation ; methods ; Surgical Flaps ; transplantation

A rapid and sensitive analytical method was developed for the simultaneous determination of fumonisins B1 and B2 in traditional Chinese medicines by HPLC-MS/MS. The detection limits for fumonisins B1 and B2 were 0.25 ng x mL(-1) corresponding to 2 microg x kg(-1) in samples. Recoveries from different samples spiked with fumonisins B1 and B2 at levels ranging from 0.2 to 3 mg x kg(-1) were 84.0%-96.1% and 86.3%-99.3%, respectively. Among the total of 34 samples purchased from local markets, ten samples of which were visibly moldy samples due to inappropriate storage, and 24 were normal samples. The results showed that 6 of the visibly moldy samples and 5 of the normal samples were contaminated with total fumonisins at levels ranging 82.4-2349 microg x kg(-1) and 102-729 microg x kg(-1), respectively.
Chromatography, High Pressure Liquid ; Drug Contamination ; Drugs, Chinese Herbal ; chemistry ; Fumonisins ; analysis ; Medicine, Chinese Traditional ; Plants, Medicinal ; chemistry ; Tandem Mass Spectrometry