1.Changes of Plasma Levels of Thrombomodulin and Its Significance in Children with Kawasaki Disease before and after Treatment
Ai-guo, LI ; Shi-wei, YANG ; Hong-chun, SU ; Chun-hua, GONG
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To explore the change and clinical significance of plasma levels of thrombomodulin(TM) in children with Kawasaki disease(KD)(n=44) before and after treatmen with intravenous immunoglobulin(IVIG).Methods Enzyme-linked immunosorbent assay(ELISA) was used to detect the plasma levels of TM in children with KD(n=44) before and after treatment with IVIG and in normal control group(n=15),respectively.Children with KD were enrolled from September 2004 to June 2006,one group(n=20) with coronary artery lesions(CALs) and another group(n=24) without CALs.The control were enrolled from heathy children in clinic service.Results The plasma level of TM in KD group before treatment with IVIG was significantly higher than that in control group(P
2.Establishment of HPLC-ESI-MS method for the determination of eplerenone in human plasma and its pharmacokinetics.
Wen-Juan QIAN ; Li DING ; Ai-Dong WEN ; Bin GONG ; Ye LENG ; Chang-Hong YUN ; Lin YANG
Acta Pharmaceutica Sinica 2009;44(7):771-777
A sensitive high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method was established for the determination of eplerenone (EP) in human plasma. The plasma samples of EP were extracted with ethyl acetate and separated by HPLC on a reversed phase C18 column with a mobile phase of 10 mmol x L(-1) ammonium acetate water solution-methanol (30 : 70, v/v). EP was determined with electrospray ionization-mass spectrometry (ESI-MS) in the selected ion monitoring (SIM) mode. The calibration curves were linear over the range of 2-4 000 ng x mL(-1) for EP. The lower limit of quantification was 2 ng x mL(-1). The method has been successfully applied in the pharmacokinetic study of the EP tablets. The main pharmacokinetic parameters of EP after oral administration of 25 mg, 50 mg, 100 mg were as follows, t1/2: (4.9 +/- 2.1), (4.7 +/- 1.5), (5.9 +/- 1.2) h; AUC(0-infinity): (4 402 +/- 1 735), (8 150 +/- 2 509), (13 783 +/- 4 102) microg x h x L(-1); and MRT: (6.2 +/- 2.1), (6.6 +/- 1.3), and (7.2 +/- 1.6) h. Parameters of EP after oral administration of multiple doses of 50 mg were as follows, t1/2: (6.1 +/- 1.7) h; AUC(ss): (10 071 +/- 4220) microg x h x L(-1); MRT: (8.1 +/- 2.3) h; and DF: (3.2 +/- 1.0).
Chromatography, High Pressure Liquid
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methods
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Humans
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Spectrometry, Mass, Electrospray Ionization
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methods
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Spironolactone
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analogs & derivatives
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blood
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pharmacokinetics
3.Studies on chemical constituents in stem of Dendrobium chrysotoxum.
Yan-Qing GONG ; Hong YANG ; Yun LIU ; Ai-Qun LIANG ; Zheng-Tao WANG ; Luo-Shan XU ; Zhi-Bi HU
China Journal of Chinese Materia Medica 2006;31(4):304-306
OBJECTIVETo investigate the chemical constituents of Dendrobium chrysotoxum.
METHODThe chemical constituents were isolated by various column chromatographic methods and structurally elucidated by spectral evidences.
RESULTTen compounds were obtained and identified as (+)-syringare sinol (1), 5alpha, 8alpha-epidioxy-24( R)-methycholesta-6, 22-dien-3beta-ol (2), trans-3-(4-hydroxy-3-methoxyphenyl)-acrylic acid octacosyl ester (3), defusin (4), 3, 4-dihydroxy benzoic acid (5), 3, 4-dimethoxy-benzoic acid (6), vanillic acid (7), 3, 4-dimethoxy-benzoic acid methyl ester (8), 3, 5-dibromo-2-aminobenzaldehyde (9), heptadecanoic acid 2, 3-dihydroxy-propyl ester (10).
CONCLUSIONCompounds 1, 2 and 6-10 were isolated from this plant for the first time.
Dendrobium ; chemistry ; Furans ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Vanillic Acid ; chemistry ; isolation & purification
4.A survey of fluid therapy in 2 intensive care units
Hong LIU ; Fang GONG ; Yuhang AI ; Meilin AI ; Qing FENG ; Songyun DENG ; Zhiyong LIU ; Lina ZHANG
Chinese Journal of Internal Medicine 2018;57(6):446-449
To explore the present status of fluid therapy and clinical outcome in critically ill patients in intensive care units (ICU).ICU patients consecutively admitted to our ICU were prospectively enrolled.Patients' demographics,laboratory data,fluid record and clinical outcome were collected.Fluid intake quantity of all patients was at peak on the fifth day which was 2 806 (1 997,3 582)ml.From the fourth day in ICU,fluid balance started to benegative as-84 (-1 127,612)ml and gradually increased.Crystalloid solution was the main components.For treatment purposes,medication injections and nutrients were major fluids.Positive correlations were found between total fluid intake quantity,total crystalloid volume,total colloidal volume and hospital stay,ICU stay,duration of intubation (r values as 0.211,0.686,0.282,0.155,0.506,0.174,0.209,0.072,0.292,respectively P<0.05).Moreover,positive correlations were also demonstrated between total colloidal volume and total bilirubin,direct bilirubin,alanine transaminase,aspartate transaminase,blood urea nitrogen,serum creatinine (r values as 0.196,0.242,0.190,0.335,0.284,0.223,respectively P<0.05).
5.Study on dosimetry of 125I seed in-plane implantation
Juan, WANG ; Hong-tao, ZHANG ; Wen-qing, ZHAO ; Wei-hong, GONG ; Teng-fei, LIU ; Zhi-hui, HU ; Jian-hua, WANG ; Jian-bo, ZHANG ; Ai-xia, SUI ; Jian-bin, XU
Chinese Journal of Nuclear Medicine 2010;30(5):339-342
Objective To study the dosimetry of different arrangements of 125I seeds in one plane.Methods Nine different in-plane arrangements of 9 125I seeds (2.035 × 107 Bq/seed) were simulated according to distance (cm) along x (horizontal)- and y( longitudinal )-axis using the 3-dimensional treatment planning system (TPS) (3D-TPS): x0.5, y0. 5; x0. 5, y1.0; x0. 5, y1.5; x1.0, y1.0; x1.0, y1.5;x1.5, y1.5; x0. 5, y0. 5 (2)1.0; x0.5, y1.0 (2)0.5; x1.0, y1.0 (2)0.5. The isodose curves of 40,80, 130, 145 and 200 Gy were created and the area, radius and medical cost under the 40, 80, 130, 145and 200 Gy isodose curves were calculated. Results The area, radius and medical cost under the same isodose curves were significantly different with each 125I seed arrangement. The arrangements which had the biggest area under curves of 40, 80, 130, 145 and 200 Gy isodose were x1. 5, y1. 5; x1. 0, y1. 0; x1. 0,y1. 0; x0. 5, y1. 0 and x0. 5, y1. 0, respectively. Conclusion The matched peripheral dose and therapeutic effect were affected significantly by the geometric arrangement of 125I seeds.
6.Evaluation of tracheal intubation in critical SARS patients.
Zhi-yi GONG ; Yu-guang HUANG ; Jing-jie WANG ; Qing XU ; Yong LI ; Xue-rong YU ; Guang-jun CHEN ; Ai-lun LUO ; Hong-zhi REN
Acta Academiae Medicinae Sinicae 2003;25(5):550-552
OBJECTIVETo analyze tracheal intubation and respiratory treatment in the critical severe acute respiratory syndrome (SARS) patients.
METHODSReview and analyze tracheal intubation and respiratory treatment in critical SARS patients in intensive care unit (ICU).
RESULTSThree of thirteen patients had been intubated or received tracheotomy before they entered into ICU, the other patients received treatment of nasal cannula or oxygen mask. With the development of the disease, two patients had been intubated because of respiratory failure or tracheotomy. Tracheal intubation was twice made in two patients in order to replace tracheal tubes.
CONCLUSIONSThe patient should be intubated or received tracheotomy if non-invasive respiratory support has no effect. Standard protection could protect medical staff from infection under tracheal intubation.
Adult ; Aged ; Aged, 80 and over ; Critical Care ; Evaluation Studies as Topic ; Female ; Humans ; Infectious Disease Transmission, Patient-to-Professional ; prevention & control ; Intubation, Intratracheal ; methods ; Male ; Middle Aged ; Severe Acute Respiratory Syndrome ; therapy ; transmission ; Tracheostomy
7.Myocardial polyamine metabolism and the ischemia-reperfusion injury in the rat heart.
Li-ping HAN ; Chang-qing XU ; Chun-ming JIANG ; Hong-zhu LI ; Ya-jun ZHAO ; Yong-sheng GONG ; You-ai DU ; Yi-min GUO
Chinese Journal of Cardiology 2008;36(4):346-349
OBJECTIVETo observe the polyamines metabolism changes in rat cardiomyocytes underwent ischemia-reperfusion (I/R) injury.
METHODSA branch of the descending left coronary artery was occluded to induce rat myocardial I/R injury (30 min ischemia followed by 2 h, 6 h, 12 h, and 24 h reperfusion). RT-PCR and Western blot were performed to detect the expression of spermidine/spermine N1-acetyltransferase (SSAT) and ornithine decarboxylase (ODC), the concentrations of polyamines were measured with high performance liquid chromatography in hearts with or without I/R.
RESULTSThe myocardial transcription and expression of SSAT and ODC were significantly upregulated. Compared with the sham group, ODC mRNA and SSAT mRNA respectively increased 3.1 fold and 3.8 fold and their proteins respectively increased 3.1 fold and 2.9 fold at 24 h of reperfusion (P < 0.01); the concentrations of spermidine, spermine and the total polyamine pool respectively decreased by 33.6%, 35.3% and 32.9% while putrescine concentration increased by 58.9% at 24 h of reperfusion (P < 0.01).
CONCLUSIONOur results suggest that ischemia-reperfusion in the heart may affect polyamine metabolism and the disturbance of polyamine metabolism might thus play a critical role in myocardial I/R injury in this model.
Animals ; Disease Models, Animal ; Female ; Male ; Myocardial Reperfusion Injury ; metabolism ; Myocardium ; metabolism ; Polyamines ; metabolism ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction
8.Mobilization of autologous peripheral blood stem cells with etoposide and recombinant human granulocyte colony stimulating factor in malignant tumor patients.
Yuan-kai SHI ; Xiao-hui HE ; Xiao-hong HAN ; Jian-liang YANG ; Peng LIU ; Chang-gong ZHANG ; Bin AI
Chinese Journal of Oncology 2004;26(6):360-363
OBJECTIVETo observe the combined effect of etoposide (Vp-16) and recombinant human granulocyte colony-stimulating factor (rhG-CSF) on mobilization of autologous peripheral blood stem cells (APBSC) in malignant tumor patients and find out the suitable dose of Vp-16.
METHODSThirty patients were randomly divided into two groups, 15 in each group. In group A, Vp-16 1000 mg/m(2) was injected intravenously in six divided doses, for 2 hours every 12 hours on day 1, 2 and 3. In group B, Vp-16 1500 mg/m(2) was injected intravenously in six divided doses for 3 hours every 12 hours on day 1, 2 and 3. rhG-CSF was given as a single daily injection subcutaneously at the dose of 300 microg.body(-1).day(-1) from the day of the nadir of white blood cell (WBC) to the day before the end of APBCS harvest. APBSC harvest started when WBC > or = 5.0 x 10(9)/L and finished when accumulated mononuclear cells (MNC) > or = 5 x 10(8)/kg or CD34+ cells > or = 2 x 10(6)/kg.
RESULTSThere was no significant difference between the time of nadir, nadir of WBC, absolute neutrophil count (ANC), the beginning time and continuous time of rhG-CSF given, the beginning time and continuous time of APBSC harvest. When the blood volume, flow rate and continuous time of apheresis were similar in each apheresis in the two groups, the number of APBSC in each harvest and total number of APBSC were also not significantly different between the two groups. The side effects induced by Vp-16 were also not significant different between the two groups.
CONCLUSIONVp-16 combined with rhG-CSF is a safe and highly effective method for APBSC mobilization, 1000 mg/m(2) and 1500 mg/m(2) of Vp-16 possess similar efficiency and side effects for APBSC mobilization.
Adolescent ; Adult ; Antineoplastic Agents, Phytogenic ; administration & dosage ; pharmacology ; Child ; Dose-Response Relationship, Drug ; Etoposide ; administration & dosage ; pharmacology ; Female ; Granulocyte Colony-Stimulating Factor ; administration & dosage ; pharmacology ; Hematopoietic Stem Cell Mobilization ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells ; drug effects ; physiology ; Hodgkin Disease ; therapy ; Humans ; Lymphoma, Non-Hodgkin ; therapy ; Male ; Middle Aged ; Recombinant Proteins
9.Perioperative anesthetic management for the excision of phaeochromocytoma complicated with catecholamine cardiomyopathy.
Xiang-yang GUO ; Ai-lun LUO ; Zhi-yi GONG ; Hong-zhi REN ; Tie-hu YIE ; Yu-guang HUANG
Acta Academiae Medicinae Sinicae 2002;24(4):424-426
OBJECTIVETo summarize experience of perioperative anesthetic management for patients undergone excision of pheochromocytoma and complicated with catecholamine cardiomyopathy.
METHODSPerioperative anesthetic management for surgical treatment of three cases of pheochromocytoma complicated with catecholamine cardiomyopathy was described and discussed according to literature reports.
RESULTSThe catecholamine cardiomyopathy of the three cases presented with left ventricular hypertrophy, congestive cardiac failure and acute myocardial infarction. After removal of the pheochromocytoma under general anesthesia, a prolonged hypotension occurred in all of the three cases. In order to maintain stable hemodynamics, large dose of catecholamine was required after surgery. All of the three patients were survived and discharged.
CONCLUSIONSIt is suggested that myocardial dysfunction may be another important factor for the prolonged hypotension after removal of the tumor. Meticulous preoperative assessment of heart function is of primary importance for the management of anaesthesia during surgical procedures.
Adrenal Gland Neoplasms ; blood ; surgery ; Adult ; Anesthesia, General ; Cardiomyopathies ; blood ; etiology ; Catecholamines ; blood ; Female ; Heart Failure ; blood ; etiology ; Humans ; Male ; Pheochromocytoma ; blood ; surgery
10.Expression of G-CSF and GM-CSF receptors on CD34 positive cells in aplastic anemia and myelodysplastic syndrome patients and its significance.
Hong-Zhi XU ; Ai LI ; Yuan YU ; Jian-Feng LI ; Xin LIU ; Xiang-Hua WANG ; Xin WANG ; Gong-Li XU
Journal of Experimental Hematology 2008;16(6):1308-1311
This study was aimed to detect the ratio of CD34+ cells in bone marrow mononuclear cells (BMMNCs) and the expression rate of G(M)-CSFR on CD34+ cells in bone marrow of the patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS). The ratio of CD34+ cells in BMMNCs and the expression rate of G(M)-CSFR on cells of 27 AA patients, 45 MDS patients and 20 controls were detected by flow cytometry (FCM). The results showed that the ratio of CD34+ cells in BMMNCs of AA patients reduced and was significantly different from controls (p<0.05), the ratio of CD34+ cells in MDS patients elevated and was significantly different from controls (p<0.05). Compared with controls and MDS-RA patients, the ratio of CD34+ cells in MDS-RAEB patients significantly elevated (p<0.05), but there was no significant difference between MDS-RA patients and controls (p>0.05). The ratio of CD34+ cells in MDS-RA patients was significantly higher than that in AA patients (p<0.05). There was no significant difference in expression rate of G-CSFR on CD34+ cells between AA patients and controls, MDS patients and controls, AA patients and MDS patients, MDS-RA patients and MDS-RAEB patients (p>0.05). The expression rate of GM-CSFR in MDS patients was significantly higher than that in AA patients and controls (p<0.05), but there was no significant difference between AA patients and controls, MDS-RA patients and MDS-RAEB patients (p>0.05). In AA patients, the ratio of CD34+ cells in BMMNCs was less than 0.1% accounts for 6/8 SAA patients, compared with 2/19 in CAA (p<0.05). There was no correlation between the expression rate of either G-CSFR or GM-CSFR and neutrophil count at diagnosis (r=0.058 and r=0.044). In MDS patients, there was no correlation between bone marrow CD34+ cells ratio and peripheral neutrophil count at diagnosis (r=-0.335). And there was no correlation between the expression of either G-CSFR or GM-CSFR and neutrophil count on diagnosis (r=0.064 and r=0.051). It is concluded the detection of CD34+ cells and their surface expression rate of G(M)-CSFR in AA and MDS is useful in diagnosis and differential diagnosis of these two diseases.
Adult
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Anemia, Aplastic
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metabolism
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Antigens, CD34
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immunology
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Bone Marrow Cells
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cytology
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immunology
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metabolism
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Case-Control Studies
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Female
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Flow Cytometry
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Humans
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Male
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Middle Aged
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Myelodysplastic Syndromes
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metabolism
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Receptors, Granulocyte Colony-Stimulating Factor
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metabolism
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
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metabolism