Objective To evaluate the value of minimally invasive technique in treatment of severe traumatic intracranial hematoma at emergency department.Method From January 1995 to December 2005,126 patients were treated by using the minimally invasive technique in Affiliated Chaoyang hospital once patients were diagnosedas severe traumatic cerebral hernia resulted from intracranial hematoma and also those whose clinical data were retrospectively analyzed.In the emergency department,the per-operative plan was done according to the CT imaging,including the sute of burr hole on the skull,the direction and depth of carmulation,and the drainage cannula was put into the hematoma cavity for external drainage under local anesthesia.Results The successful rate of puncture was 100%.The patients's dukated oyouk contracted immediately after drainage.There are 79/ 95 patients(83.1%)had single pupil dilated and 17/31(54.8%)patients had bilateral pupils dilated.The immediately clinical effective was 76.2%.According to Glasgow outcome scale,43 of 126 patients had good recovery,26 had moderate deficits and 18 had severe sequelae,16 patients were in vegetative state,18 died. Conclusions Clinical prospective study proves that minimally invasive technique can ameliorate the cerebral hernia,prolong the operative therapie window time.

OBJECTIVEThis study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC.

METHODThe HPLC analysis was performed on an Agilent Eclipse Plus C18 column (2.1 mm x 150 mm, 1.8 μm) with 0.1% fomic acid aqueous solution-0.1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0.3 · mL(-1) · min(-1); The detective wavelength is 325 nm; The column temperature is 45 °C.

RESULTThe results indicated that 5 of 17 common peaks were identified . The similarity about 10 groups of Inulacappais is over 0.95.

CONCLUSIONThis method is able to be a scientific basis of quality assessment according to its convenient and reliable.

Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Inula ; chemistry

Using in vitro everted gut to investigate the intestinal absorption of the extracts from Polygonum orientale at different concentration. UPLC-MS/MS was used to detect the content of protocatechuic acid, isoorientin, orientin, vitexin, cynaroside, quercitrin, kaempferol-rhamnoside in different intestinal segments, then compared the results with the absorption of chemical components of extractive P. orientale in each intestinal segments, and calculated the absorption parameter. We took the statistic analysis with SPSS statistic software. The influence significance of each factors were analyzed to describe the character of absorption. The absorption of each component is linearity in different intestinal segments and different dose, and the square of coeficient correlation exceed 0.95, which consistent with zero order rate process. The K(a) increase along with the raised dosage of the extractive P. orientale (R2 > 0.95), indicated it is the passive absorption; different intestinal segments have different absorption. And the absorption trend in intestinal is duodenum, jejunum, ileum are greater than the colon. As ingredients are selectively absorbed in intestinal sac, the everted intestinal sac method is selected to assess the intestinal absorption charcteristics of ingredients of extractive P. orientale.
Animals ; Drugs, Chinese Herbal ; pharmacokinetics ; Gastrointestinal Tract ; metabolism ; Intestinal Absorption ; Male ; Polygonum ; chemistry ; metabolism ; Rats ; Rats, Sprague-Dawley

Objective:To explore the mediating effect of coping efficacy on the relationship between psychological stress and adjustment.Methods:This study was a longitudinal study.Totally 267 college freshmen (137 males and 130 females,aged 19 to 21) were selected to fill in the China College Student Psychological Stress Scale (CCSPSS),China College Student Adjustment Scale (CCSAS) and Coping Efficacy Questionnaire (CEQ),each measurement interval two months,three times totally.Results:The CCSPSS scores were negatively correlated with scores of CCSAS and CEQ cognitive dimension (r =-0.29--0.08,Ps ＜ 0.05),and positively correlated with measurement period (represented by a continuous and dummy variable,r =0.08,P ＜ 0.05).The CCSAS scores were positively correlated with scores of CEQ cognitive dimension (r =0.25,P ＜ 0.01),and negatively correlated with measurement period (r =-0.13,P ＜ 0.01).The CEQ cognitive dimension scores was negatively correlated with measurement period (r =-0.14,P ＜ 0.01).The mediating effect analysis showed that coping efficacy played a partly mediating role between adjustment and psychological stress,measurement time,and the mediating effect were accounted for the total effect 45.7％,33.6％.Conclusion:It suggests that psychological stress and measurement time may not only directly affect the adjustment,but also indirectly affect adjustment through mediating effect of coping efficacy.

OBJECTIVETo investigate maternal-fetal transmission at human bocavirus (HBoV).

METHODSIgG antibody to HBoV in serum samples of 316 mothers were determined with ELISA and HBoV DNA was determined with real time PCR in the sera of the mothers and their infants.

RESULTSHBoV-IgG was positive in 40.20 percent (127/316) of the mothers, while it was positive in 29.43 percent (93/316) of the cord blood specimens of the infants. The difference between the two groups was significant (X2=8.12, P less than 0.005); 93 samples of both the mothers and the infants were positive for HBoV-IgG.

CONCLUSIONHBoV-IgG can cross the placenta to the fetuses through placenta. Further study is needed to answer the question whether vertical maternal-fetal transmission occurs.

Adult ; Antibodies, Viral ; blood ; Bocavirus ; DNA, Viral ; blood ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin G ; blood ; Infant, Newborn ; Infectious Disease Transmission, Vertical ; Parvoviridae Infections ; transmission ; Pregnancy ; Prospective Studies

Objective To study the effect of enalapril folic acid on peripheral blood inflammatory factors in type H hypertensive of rats.Methods Sixty Wistar Spontaneous hypertension male rats were randomly divided into control group,model group,enalapril group and enalapril folic acid group,15 rats in each group.Type H hypertensive rat model were established by feeding with 3％ methionine in model group,enalapril group and enalapril folic acid group.And enalapril folic acid group were given 10 mL · kg-1 enalapril maleate folic acid solution,enalapril group were given 10 mL · kg-1 enalapril solution,and the model group were given 10 mL · kg-1 double distilled water,and 4 groups were intervened for 8 weeks everyday.The serum levels of homocysteine (Hcy),serum tumor necrosis factor alpha (TNF-α),hypersensitivity C reactive protein (hs-CRP)and soluble OX40 ligand(OX40L) were measured by enzyme-linked immunosorbent assay.The nitric oxide(NO)level were measured by End-point assay.The structure of carotid artery was observed under microscope.Results Compared with Hcy(64.22 ±6.82) μ mol · L-1 and NO(41.45 ±5.25) μmol · L-1 in the enalapril group,levels of Hcy(19.75 ±2.14) μmol · L-1 were lower,and NO(57.68 ±6.43) μmol · L-1were higher in the enalapril folic acid group with significantly (P ＜ 0.05).Compared with TNF-α (72.72 ± 6.89) pg · mL-1,hs-CRP (1.15 ±0.12) pg · mL-1,OX40L(5.24 ±0.47) ng · mL-1in the enalapril group,levels of TNF-α(58.18 ±5.76)pg · mL-1,hs-CRP(0.86-±0.11) pg · mL-1,OX40L(3.03 ±0.39) ng · mL-1 were lower in the enalapril folic acid group with significantly(P ＜0.05).Compared with the carotid artery IMT (61.14±7.73) μm,and IMCSA levels(0.12 ± 0.02) mm2 in the enalapril group,the carotid artery IMT (52.74±6.29)μm,and IMCSA (0.10 ±0.01) mm2 in the enalapril folic acid group were lower with significantly(P ＜ 0.05).Compared with the levels of carotid artery LD(951.38 ± 6.08) μm in the enalapril group,the levels of carotid LD (969.79 ± 6.17) μm in the enalapril folic acid group was higher with significantly(P ＜0.05).Conclusion enalapril folic acid can effectively reduce levels of peripheral blood Hey,TNF-oα,hs-CRP and OX40L in type H hypertensive rats,improve levels of NO,reduce inflammation,and improve the structure of carotid artery.

OBJECTIVECleidocranial dysplasia (CCD) is a rare skeletal disease with autosomal dominant inheritance associated with mutation in RUNX 2. The authors report a Chinese girl with CCD in whom the mutation in RUNX 2 was identified.

METHODSClinical diagnosis was based on physical examination, radiological findings, and biochemical tests. For mutation detection, genomic DNA was extracted from peripheral blood using standard method. All 7 coding exons of RUNX 2 and their flanking intronic sequences were amplified by polymerase chain reaction (PCR), and the PCR products were then subjected to automatic DNA sequencing.

RESULTSThe affected girl showed typical clinical manifestations of CCD, including patent fontanelles, absent clavicles, short stature and dental anomalies. Direct sequencing of PCR-amplified fragments revealed a recurrent missense mutation, R190W (568 C > T), in RUNX 2. The mutation was further confirmed by Hae III restriction analysis.

CONCLUSIONA Chinese case of CCD was confirmed and the disease-causing mutation was linked to a recurrent point mutation in RUNX 2.

Cleidocranial Dysplasia ; genetics ; Core Binding Factor Alpha 1 Subunit ; genetics ; Female ; Humans ; Mutation

OBJECTIVE: To profile urinary metabolite variations from 1, 2-dimethylhydrazine (DMH)-induced precancerous colon rats, Jinfu Kang treated rats and healthy controls.

METHODWe used ethyl chloroformate derivatization and gas chromatography-mass spectrometry (GC-MS) based metabonomic method to analyze rat urines.

RESULTThe time-dependent variations of metabolite profile showed a progressive deviation of the metabolism in the model group from the initial pattern over time and a systemic recovery of the metabolism in the treatment group, which is consistent with the histological results. The in-depth analysis indicated that the disorder of tricarboxylic acid cycle (TCA), tryptophan metabolism, polyamine metabolism and gut flora structure were associated with DMH intervention.

CONCLUSIONMetabolic study revealed that Jinfu Kang can effectively reverse metabolic departures in DMH-induced precancerous colon rat, which is consistent with pathological results.

Animals ; Colonic Neoplasms ; chemically induced ; pathology ; Colonic Polyps ; chemically induced ; drug therapy ; urine ; Dimethylhydrazines ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Gas Chromatography-Mass Spectrometry ; Male ; Rats ; Rats, Wistar

Humans ; Male ; Middle Aged ; POEMS Syndrome ; diagnosis ; Syphilis ; diagnosis

OBJECTIVETo study the changes of peripheral plasmacytoid dendritic cells and lymphocyte subsets in patients with chronic hepatitis B (CHB) during consensus interferon (CIFN) treatment.

METHODSTwenty-three patients with CHB were treated with CIFN for 24 weeks and followed up for another 24 weeks. Peripheral plasmacytoid dendritic cells and lymphocyte subsets were measured throughout the treatment and follow-up periods.

RESULTSAfter CIFN treatment, 43.5% of the patients had virological and biochemical responses. The percentage and absolute number of peripheral plasmacytoid dendritic cells decreased significantly (P less than 0.05), the number of CD3+ cells, CD4+ T cells, B cells and the ratio of CD4+/CD8+ cells decreased also (P less than 0.05), but the number of CD8+ T cells and NK cells increased (P less than 0.05).

CONCLUSIONSConsistent virological and biochemical responses can be seen in some patients with CHB virus infection after CIFN treatment, and the percentage and number of their peripheral plasmacytoid dendritic cells greatly decreased, but the number of CD8+ T cells and NK cells increased.

Adult ; Antiviral Agents ; therapeutic use ; CD8-Positive T-Lymphocytes ; immunology ; Dendritic Cells ; immunology ; Female ; Hepatitis B, Chronic ; drug therapy ; immunology ; Humans ; Interferon-alpha ; therapeutic use ; Killer Cells, Natural ; immunology ; Male ; Young Adult