A fluorescence method is found for determination of bovine serum albumin (BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2. The complex exitation wavelength is 467 nm, the emission wavelength is 515 nm. The linear measurement rang is between 1.8-500 mg/L for BSA, F = 26.776 C + 2.8082, r = 0.9999. This method is sensitive,steady,and low cost for determination of BSA.
Animals ; Cattle ; Drug Interactions ; Fluorescein ; chemistry ; Serum Albumin, Bovine ; analysis ; Spectrometry, Fluorescence ; methods