1.Effect of IL-18 binding protein on hepatic ischemia-reperfusion injury induced by infrarenal aortic occlusion.
Mustafa OZSOY ; Yucel GONUL ; Ahmet BAL ; Ziya Taner OZKECECI ; Ruchan Bahadir CELEP ; Fahri ADALI ; Omer HAZMAN ; Ahmet KOCAK ; Murat TOSUN
Annals of Surgical Treatment and Research 2015;88(2):92-99
PURPOSE: Severe local and systemic tissue damage called ischemia/reperfusion (IR) injury occurs during the period of reperfusion. Free oxygen radicals and proinflammatory cytokines are responsible for reperfusion injury. IL-18 binding protein (IL-18BP) is a natural inhibitor of IL-18. The balance between IL-18 and IL-18BP has an important role in the inflammatory setting. The present study aimed to investigate whether IL-18BP had a protective role in remote organ hepatic IR injury. METHODS: Wistar-Albino rats were divided into three groups that contained seven rats. Group I (sham): Laparotomy and infrarenal abdominal aorta (AA) dissection were done but no clamping was done. Group II (I/R): The infrarenal AA was clamped by atraumatic microvascular clamp for 30 minutes and then was exposed to 90 minutes of reperfusion. Group III (IR + IL-18BP): 75 microg/kg of IL-18BP in 0.9% saline (1 mL) was administered 30 minutes before infrarenal AA dissection and clamping; 30 minutes of ischemia was applied and then was exposed to 90 minutes of reperfusion. RESULTS: Serum AST, ALT, and LDH levels were remarkably higher in IR group and returned to normal levels in treatment group. The proinflammatory cytokine levels had decreased in treatment group, and was statistically significant compared with the IR group. Serum levels of total oxidant status and oxidative stress index decreased and levels of total antioxidant status increased by IL-18BP. CONCLUSION: This study suggested that IL-18BP has antioxidant, anti-inflammatory and hepatoprotective effects in cases of IR with infrarenal AA induced liver oxidative damage.
Animals
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Aorta, Abdominal
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Carrier Proteins*
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Constriction
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Cytokines
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Interleukin-18*
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Ischemia
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Laparotomy
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Liver
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Oxidative Stress
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Rats
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Reactive Oxygen Species
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Reperfusion
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Reperfusion Injury*
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Transplantation
2.Gastric Carcinoma with Bone Marrow Metastasis: A Case Series.
Ahmet Siyar EKINCI ; Oznur BAL ; Tahsin OZATLI ; Ibrahim TURKER ; Onur ESBAH ; Ayse DEMIRCI ; Burcin BUDAKOGLU ; Ulku Yalcintas ARSLAN ; Emrah ERASLAN ; Berna OKSUZOGLU
Journal of Gastric Cancer 2014;14(1):54-57
Gastric cancer is a major cause of cancer-related mortality. At the time of diagnosis, majority of the patients usually have unresectable or metastatic disease. The most common sites of metastases are the liver and the peritoneum, but in the advanced stages, there may be metastases to any region of the body. Bone marrow is an important metastatic site for solid tumors, and the prognosis in such cases is poor. In gastric cancer cases, bone marrow metastasis is usually observed in younger patients and in those with poorly differentiated tumors. Prognosis is worsened owing to the poor histomorphology as well as the occurrence of pancytopenia. The effect of standard chemotherapy is unknown, as survival is limited to a few weeks. This report aimed to evaluate 5 gastric cancer patients with bone marrow metastases to emphasize the importance of this condition.
Bone Marrow*
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Diagnosis
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Drug Therapy
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Humans
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Liver
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Mortality
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Neoplasm Metastasis*
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Pancytopenia
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Peritoneum
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Prognosis
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Stomach Neoplasms