1.Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model.
Ali AKDEMIR ; Burak ZEYBEK ; Levent AKMAN ; Ahment Mete ERGENOGLU ; Ahmet Ozgur YENIEL ; Oytun ERBAS ; Altug YAVASOGLU ; Mustafa Cosan TEREK ; Dilek TASKIRAN
Journal of Gynecologic Oncology 2014;25(4):328-333
OBJECTIVE: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. METHODS: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays. RESULTS: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. CONCLUSION: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
Animals
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Anti-Mullerian Hormone/blood
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Antineoplastic Agents/*toxicity
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Biological Markers/blood
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Cisplatin/*toxicity
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Disease Models, Animal
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Drug Evaluation, Preclinical/methods
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Female
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Fertility Preservation/methods
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Granulocyte Colony-Stimulating Factor/*therapeutic use
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Ovarian Follicle/drug effects/pathology
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Primary Ovarian Insufficiency/blood/chemically induced/pathology/*prevention & control
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Rats, Sprague-Dawley