1.Wild-tending techniques study on Glycyrrhiza uralensis--effect of irrigation and rhizome length on survival ratio, yield and quality.
Li LI ; Sheng-Li WEI ; Wen-Quan WANG ; Hua SHI ; Sogumeyi TODA ; Zheng-Zheng GUO ; Guang-Xi REN
China Journal of Chinese Materia Medica 2014;39(15):2863-2867
This research aimed at studying the effects of irrigation and rhizome length on the survival of ratio, yield and quality of Glycyrrhiza uralensis in wild tending condition. Employed the split-block design to carry out the field experiment, sampled with the quadrat method to measured the relative growth indexes and to estimate the yield, used the HPLC (high performance liquid chromatog- raphy ) method to measure the glycyrrhizin in the rhizome and adventitious root of the G. uralensis in this study. The quantity of the adventitious roots and the survival ratio were increased significantly as the length of the rhizome increased (P < 0.01), but the length of the rhizome had no remarkable effect on the content of glycyrrhizin. The average content of the glycyrrhizin in the adventitious root and rhizome could reach 3.03% and 2.12% after 3-year wild tending, respectively, and this results indicated that the quality of the glycyrrhiza using this method was much better than that from cultured glycyrrhiza with the reproducing method of seeding. so using the rhizome as reproductive material to produce the glycyrrhiza under the wild tending condition could get the high quality glycyrrhiza quick- ly and steadily, this phenomenon could be explained by the Hypothesis of synthetic inertia of the medicinal components from the wild material of G. uralensis. But the maximum yield with this method was just more than 945 kg x hm(-2) in this study. So the further work of how to increase the yield in the practical application with the method found in this study need to be done in the next research.
Agricultural Irrigation
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Culture Techniques
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methods
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Glycyrrhiza uralensis
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growth & development
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metabolism
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Glycyrrhizic Acid
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metabolism
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Rhizome
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growth & development
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Survival Analysis
2.Physiological ecology responses of Scutellaria baicalensis to drought and rewatering.
Yong-Gang ZHANG ; Mei HAN ; Xue JIANG ; Sheng-Nan ZHAO ; Li-Min YANG
China Journal of Chinese Materia Medica 2013;38(22):3845-3850
To study the physiological ecology responses of Scutellaria baicalensis to drought and rewatering of short period, we tested and analyzed photosynthesis and chlorophyll fluorescence parameters of S. baicalensis leaves processed by different ways of water treatment in drought and rewatering period, characteristic indexes of physiology and biochemistry of root SOD, POD, PAL, C4H, etc. and accumulation dynamic change of root baicalin and baicalein. The result showed that along with the worsening drought, P(n), T(r), G(s) and F(v)/F(m) of S. baicalensis declined in different water supply, and F(o) increased. The response of SOD and POD's activity in S. baicalensis root to drought in I and II was earlier than it in III. The response time and increase range of baicalin accumulation existed differences in different water supply, and the indexes regained after rewatering. Therefore, photosynthesis of S. baicalensis changed and it destroyed the antioxidant metabolism balance when soil water content decreased resulting from drought. The synergistic effect of defence mechanism launched by S. baicalensis, SOD, POD, PAL, C4H, baicalin and baicalein reduced active oxygen's damage to the cell.
Agricultural Irrigation
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Chlorophyll
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metabolism
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Droughts
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Drugs, Chinese Herbal
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analysis
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metabolism
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Photosynthesis
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Plant Proteins
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metabolism
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Scutellaria baicalensis
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chemistry
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enzymology
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metabolism
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Water
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metabolism