1.Cloning and protein expression of Actinobacillus actinomycetemcomitans cytolethal distending toxin subunit CdtA.
Sun Young KO ; Dong Keun JEONG ; So Hyun RYU ; Hyung Seop KIM
The Journal of the Korean Academy of Periodontology 2007;37(Suppl):339-351
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Clone Cells*
;
Cloning, Organism*
2.The effect of Actinobacillus actinomycetemcomitans lipopolysaccharide on rat periodontal tissues.
Chong Cheol KIM ; De Zhe CUI ; Young Joon KIM
The Journal of the Korean Academy of Periodontology 2007;37(Suppl):297-310
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Animals
;
Osteoclasts
;
Rats*
3.Actinobacillus actinomycetemcomitans Indeces Apoptosis of Jurkat Cell Line Through the Cleavage of Poly (ADP-ribose) Polymerase.
Sang Hwa LEE ; Su Yeong SEO ; Su Jin JEONG ; Seung Ho YOO ; Sun Mee PARK ; Min Ho JEONG ; Sung Tae YEE ; Jung Man KIM
Journal of the Korean Society for Microbiology 1998;33(5):507-519
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Apoptosis*
;
Humans
;
Jurkat Cells*
4.Study on the counting of Streptococcus mutans, Streptococcus sanguis, Haemophilus actinomycetemcomitans by methyl thiazolyl tetrazolium colorimetric method.
Zhong-chao WANG ; Li-yuan FAN ; Jun-qiang JIANG ; Wei CAI ; Yi DING
West China Journal of Stomatology 2010;28(3):306-310
OBJECTIVETo explore the feasibility of methyl thiazolyl tetrazolium (MTT) colorimetric method and the applied condition for the normal bacteria in the mouth, as Streptococcus mutans (S. mutans), Streptococcus sanguis (S. sanguis), Haemophilus actinomycetemcomitans (H. actinomycetemcomitans).
METHODSColony forming units (CFU) which was the standard antitheses was used to count bacteria. This study would gain some parameters by changing wavelength, reactive time, dosage and so on. MTT colorimetric method was applied in the counting of S. mutans, S. sanguis and H. actinomycetemcomitans.
RESULTSWhen counting S. mutans, the best wavelength was 510 nm, the best range was 1.5 x 10(5) - 1.0 x 10(7) CFU x mL(-1). When counting S. sanguis, the best wavelength was 545 nm, the best range was 1.5 x 10(5) - 2.0 x 10(7) CFU x mL(-1). When counting H. actinomycetemcomitans, the best wavelength was 557 nm, the best range was 1.0 x 10(6) - 5.0 x 10(7) CFU x mL(-1). MTT colorimetric method can be used for different aged S. mutans, S. sanguis and H. actinomycetemcomitans.
CONCLUSIONOral bacteria could be counted by MTT colorimetric method, which is fast and convenient.
Aggregatibacter actinomycetemcomitans ; Bacteria ; Humans ; Saliva ; Streptococcus mutans ; Streptococcus sanguis
5.In Vitro Antibacterial Effect of a Mouthrinse Containing CPC (Cetylpyridinium Chloride), NaF and UDCA(ursodeoxycholic acid) against Major Periodontopathogens.
Chong Kwan KIM ; Bong Kyu CHOI ; Yun Jung YOO ; Sang Nyun KIM ; Jae Kyun SEOK ; Moon Moo KIM
The Journal of the Korean Academy of Periodontology 1999;29(2):325-332
The antibacterial efficacy of a mouthrinse(Denta Gargle) containing CPC(cetylpyridinium chloride), NaF and UDCA(ursodeoxycholic acid), on major periodontopathogens, was in vitro examined and compared with that of Listerine by a broth dilution method. The bacteria tested were Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Fusobacterium nucleatum subsp. vincentii, Prevotella intermedia, Porphyromonas gingivalis and Treponema denticola. The growth of all the bacteria were completely inhibited by a 1-min exposure to the both mouthrinses. When diluted at 1:5 or more, all bacteria analyzed but P. intermedia were not inhibited by Listerine. In contrast, Denta Gargle showed highly increased maximum inhibitory dilutions(MID) against all periodontopathogens included in this study, with MIDs ranging from 5-fold(F. nucleatum) to 160-fold dilutions(P. intermedia). The MIDs against A. actinomycetemcomitans, B. forsythus, P. gingivalis and T. denticola. were 1:40, 1:80, 1:80 and 1:80, respectively.
Aggregatibacter actinomycetemcomitans
;
Bacteria
;
Bacteroides
;
Cetylpyridinium
;
Fusobacterium nucleatum
;
Porphyromonas gingivalis
;
Prevotella intermedia
;
Treponema denticola
6.The IgG subclass responses in the phenotypic subsets of the early-onset periodontitis.
The Journal of the Korean Academy of Periodontology 1999;29(1):251-264
The aim of the study was to see the total IgG and IgG subclass responses against Aa and Pg in the four early onset periodontitis (EOP) subforms or adult periodontitis (AP). 6 patients consisting of 3 patients from subform I (distinctive LJP pattern), 19 from subform II (post-juvenile periodontitis pattern), 16 from subform III ( LJP pattern but rapidly progressing), 24 from age-matched AP (20-40 years of age) have been selected for the measurements of the total IgG and each IgG subclass against to Pg and the IgG subclass against Aa, respectively. The total IgG titers against to Pg of the subforms I & III had a significantly higher values than subforms II and IV (P<0.05). Among the IgG subclasses, only the lgG3 levels were significantly higher in the subform I than the subform IV(P <0.05). Wide ranges of the antibody titers were noted in all of the EOP subforms and the AP. Except for the subform I, which was typical of localized form, the IgG2 subclass levels to Pg gradually became higher in accordance with the subforms II, III and IV. Both of IgG2 and the IgG4 antibody levels of the EOP were significantly higher than those of AP, while other subclasses were not. All of the four IgG subclass levels to Pg were consistently found to be higher in the younger age group around 20. The levels found to be low around the thirties and then gradually became higher at the ages of late thirties. The IgG2 titer to Aa in the subform I was significantly higher than those of any other subforms. Combinations of IgG1+2+4 were the most frequently found to be elevated followed by the IgG4 only, the IgG2 only, the IgG2+4, the IgG2+3+4, and the IgG1 only, in the descending order.
Aggregatibacter actinomycetemcomitans
;
Aggressive Periodontitis*
;
Chronic Periodontitis
;
Humans
;
Immunoglobulin G*
;
Periodontitis
;
Porphyromonas gingivalis
7.Antimicorbial effect of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogen and effect on human gingival fibroblast cellular activity.
Tae Il KIM ; Eun Jeong CHOI ; Chong Pyoung CHUNG ; Soo Boo HAN ; Young KU
The Journal of the Korean Academy of Periodontology 2002;32(1):249-255
Zea Mays L. has been known to be effective for improving tissue health and Magnoliae cortex to have effective antibacterial and antimicrobial activity against pathogenic microbes. The purpose of this study was to examine the antimicrobial effects of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogens(Prevotella intermedia, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans )and to examine the effects on human gingival fibroblast cellular activity. Zea Mays L. and Magnoliae cortex extracts and their mixtures were prepared with various mixing ratios (0.5:1, 1:1, 1.5:1, 2:1). These extracts were loaded to periodontal pathogen cultured petri dish for antimicrobial test and also loaded to cultured human gingival fibroblast for cellular activity test. Each test was repeated 3 times and data were analyzed by one-way ANOVA with 95% confidence level. Mixture of these two extracts showed greater amount of inhibition area on periodontal pathogen and more improved gingival fibroblast activity as Zea Mays L. ratio reduced. So, mixture ratio 0.5:1 (Zea Mays L. : Magnoliae cortex) group showed statistical significance in antimicrobial activity and cellular activity among various mixtures(p<0.05). In conclusion, 0.5:1 (Zea Mays L. : Magnoliae cortex) mixture possessed best gingival fibroblast cellular activity and antimicrobial activity toward periodontal pathogens.
Aggregatibacter actinomycetemcomitans
;
Fibroblasts*
;
Humans*
;
Magnolia*
;
Porphyromonas gingivalis
;
Streptococcus mutans
;
Zea mays*
8.Effect of Sub-Minimal Inhibitory Concentration Antibiotics on Morphology of Periodontal Pathogens.
International Journal of Oral Biology 2014;39(2):115-120
Minimal inhibitory concentration (MIC) is the lowest concentration of antibiotics that inhibits the visible growth of a microorganism. It has been reported that sub-MIC of antibiotics may result in morphological alterations along with biochemical and physiological changes in bacteria. The purpose of this study was to examine morphological changes of periodontal pathogens after treatment with sub-MIC antibiotics. Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Porphyromonas gingivalis were used in this study. The MIC for amoxicillin, doxycycline, metronidazole, penicillin, and tetracycline were determined by broth dilution method. The bacterial morphology was observed with bright field microscope after incubating with sub-MIC antibiotics. The length of A. actinomycetemcomitans and F. nucleatum were increased after incubation with metronidazole; penicillin and amoxicillin. P. gingivalis were increased after incubating with metronidazole and penicillin. However, F. nucleatum showed decreased length after incubation with doxycycline and tetracycline. In this study, we observed that sub-MIC antibiotics can affect the morphology of periodontal pathogens.
Aggregatibacter actinomycetemcomitans
;
Amoxicillin
;
Anti-Bacterial Agents*
;
Bacteria
;
Doxycycline
;
Fusobacterium nucleatum
;
Metronidazole
;
Penicillins
;
Porphyromonas gingivalis
;
Tetracycline
9.Influence of the Sonic Power Toothbrush on Reduction of Gingival inflammation and on the Amount of Interleukin-6, Prevotella intermedia and Actinobacillus actinomycetemcomitans in Periodontal Pocket.
Ji Youn HONG ; Gyung Joon CHAE ; Sung Won JUNG ; Yoo Jung UM ; Seong Ho CHOI ; Chong Kwan KIM
The Journal of the Korean Academy of Periodontology 2007;37(Suppl):409-426
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Inflammation*
;
Interleukin-6*
;
Periodontal Pocket*
;
Prevotella intermedia*
;
Prevotella*
10.Study of adherence of periodontal pathogens to collagen-treated hydroxyapatite.
Chang-juan SUN ; He YANG ; Chao-feng LEI ; Yi XU ; Ya-fei WU
West China Journal of Stomatology 2008;26(3):331-333
OBJECTIVEThe ability of oral bacteria to adhere to tooth surface is associated with their pathogenicity. The objective of this study was to compare the ability of 4 strains of periodontal pathogens attaching to collagen-treated hydroxyapatite (C-HA) beads in order to evaluate the ability of the main periodontal pathogens to form the biofilm on root surface.
METHODSThe binding amount and binding percentage of 4 strains to C-HA were measured and compared by 3H-labeled binding assay. 4 strains of periodontal pathogens were Fusobacterium nucleatum (F. nucleatum) ATCC 10953, Porphyrin gingivalis (P. gingivalis) ATCC 33277, Prevotella intermedia (P. intermedia) ATCC 25611 and Hemophilic actinomycetemcomitans (H. actinomycetemcomitans) ATCC 29523.
RESULTSThe differences of the percentage of relative adherence between F. nucleatum ATCC 10953 and P. gingivalis ATCC 33277, as well as between H. actinomycetemcomitans ATCC 29523 and P. intermedia ATCC 25611 could not be observed. However, the percentage of relative adherence of F. nucleatum ATCC 10953 and P. gingivalis ATCC 33277 was higher than that of P. intermedia ATCC 25611 and H. actinomycetemcomitans ATCC 29523 (P<0.001), no matter cultured 24 h or 48 h. No significant difference of the percentage of the relative adherence of each stain between 24 h and 48 h cultured time could be found.
CONCLUSIONF. nucleatum and P. gingivalis exhibited strong binding ability to C-HA. Their adherence to root surface may play an important role in their local aggregation, biofilm formation during the development and recurrence of the periodontitis.
Aggregatibacter actinomycetemcomitans ; Bacteria ; Collagen ; Durapatite ; Fusobacterium nucleatum ; Humans ; Periodontitis ; Porphyromonas gingivalis ; Prevotella intermedia