BACKGROUND: Type and screen is recommended for efficient use of blood and reduction in workload in blood bank. Column agglutiation test is standardized and easy to perform, and provide clear and stable reactions that improve the interpretation of results. In this study, we compared column agglutination test(Ortho Diagnostic Systems Inc., USA) with conventional tube test and investigate its usefulness in irregular antibody screening and identification. METHODS: A total 182 samples were screened for irregular antibodies using column agglutination test and conventional tube test. And 18 patient's sera in which irregular antibodies previously screened by both tube and column agglutination tests were identified for irregular antibodies by tube and column agglutination tests. We compared the results of two tests. RESULTS: In the screening test, there was 96.7%(176/182) agreements between column agglutination test and conventional tube test. The column agglutination test showed stronger reactivity than tube test. In the irregular antibody identification, there was 88.8%(16/18) agreement between two tests and disagreement were seen in the identification of anti-P1 and anti-Leb antibodies. CONCLUSION: The results of column agglutination test are objective and superior to the conventional tube test in irregular antibody screening and identification tests. These results suggest that the column agglutination test will be useful and more convenient test in antibody screening and identification.
Agglutination Tests* ; Agglutination* ; Antibodies ; Blood Banks ; Mass Screening*

We experienced two AB3 and a B3 from a 27-year-old student and his family. B3 subgroup was confirmed by delayed and weak mixed-field agglutination with anti-B serum, adsorption-elution test, serum and saliva hemagglutination inhibition test and family study. We report a family case of AB3 and B3 with brief review of literatures.
Adult ; Agglutination ; Hemagglutination Inhibition Tests ; Humans ; Saliva

We experienced two AB3 and a B3 from a 27-year-old student and his family. B3 subgroup was confirmed by delayed and weak mixed-field agglutination with anti-B serum, adsorption-elution test, serum and saliva hemagglutination inhibition test and family study. We report a family case of AB3 and B3 with brief review of literatures.
Adult ; Agglutination ; Hemagglutination Inhibition Tests ; Humans ; Saliva

Agglutination Tests ; HIV Infections ; diagnosis ; virology ; Immunoblotting ; Immunologic Tests

We studied antisperm antibody by the use of the tray agglutination test in sera of 37 vasectomized males and 65 infertile males Antisperm antibody was found in 27 out of 37 vasectomized males (73%). Antibody titer was more than 1:32 in majority of the patients (25/27). The most common agglutination pattern was tail-to-tail agglutination (70.5 %). Antisperm antibody was found in 7 out of 17 obstructive azoospermia (41.2 %), and 3 out of 42 idiopathic infertility (7.1 %). But none was found in 6 patients with varicocele.
Agglutination ; Agglutination Tests ; Azoospermia ; Humans ; Infertility ; Infertility, Male ; Male* ; Varicocele ; Vasectomy

BACKGROUND: Unexpected antibody screening and identification tests are very important for safe blood transfusion. The micro-column agglutination test (MCAT) is widely used due to its simplicity and efficiency for detecting alloantibodies. We analyzed the frequency of unexpected antibodies at three university hospital blood banks, which use two different MCAT systems. METHODS: From February 2002 to December 2009, a total of 295,876 unexpected antibody screening tests were performed at three university hospital blood banks. Two hospital blood banks (Anam and Ansan Hospitals) used the DiaMed-ID system (DiaMed Ag, Switzerland) and the other (Guro Hospital) used the Ortho BioVue system (Ortho-Clinical Diagnostics, USA) for antibody screening and identification tests. RESULTS: The rates of detecting unexpected antibodies on screening test based on the 'tests performed' and the 'persons tested' were 1.16% per test and 0.96% per person in Korea University Guro Hospital, 0.65% and 0.41% in Korea University Anam Hospital and 0.76% and 0.57% in Korea University Ansan hospital, respectively. There were significant differences in the frequencies based on the two different systems (P<0.001). Among the warm antibodies, Rh antibodies were more frequently detected by the DiaMed-ID system, and Lewis antibodies were most frequently detected by the Ortho BioVue System. CONCLUSION: We should carefully interpretate the frequency of unexpected antibodies in the Korean population because the frequencies of unexpected antibodies are different according to different employed micro-column agglutination systems.
Agglutination ; Agglutination Tests ; Antibodies ; Blood Banks ; Blood Transfusion ; Humans ; Isoantibodies ; Korea ; Mass Screening ; Phenytoin

An extensive variety of methods has been used to detect antisperm antibodies in infertile individuals, and this reflects a concern about the immunological validity, interpretation and standardization of the tests. Comparisons of different methods using the same test materials have shown little correlation between results. The purpose of this Study is to compare the results of three methods. ELISA, gelatin agglutination test and sperm immobilization test-using the same test materials based on recommendations from WHO workshop. The results are as follows: 1. Ten normal controls showed negative reactions in all the 3 tests. Out of 34 patients, the positive sera were noted in 23 (67.6%) on ELISA test, 20 (58.8%) on gelatin agglutination test and 18 (52.9%) on sperm immobilization test. 2. Fifteen (44.1 %) out of 34 patients showed positive reactions in all the 3 tests, and 26 (76.5%) out of 34 patients showed positive reaction in one or more tests. 3. The titers of the antisperm antibodies were higher in the following orders; ELISA, gelatin agglutination test and sperm immobilization test. Therefore, it seems to be possible to increase the detectability of the antisperm antibodies, if more than one tests are impolyed.
Agglutination Tests* ; Agglutination* ; Antibodies ; Education ; Enzyme-Linked Immunosorbent Assay* ; Gelatin* ; Humans ; Immobilization* ; Infertility ; Male* ; Spermatozoa*

BACKGROUND: Blood typing is an essential test for transfusion. Generally, blood typing is performed using a slide test, tube test or microcolumn agglutination test. The aims of this study were to develop a new blood typing kit using micromachining, microfluidics and microseparation methods, and to evaluate the clinical usefulness of the new blood typing kit. METHODS: We designed and manufactured a blood typing microchip using polydimethylsiloxane (PDMS), which contained a microchannel (25~200 micrometer). The blood sample and antisera to be tested were dropped on the microwell for movement and mixing by capillary action. Once agglutination occurred, the microchannel acts as a filter and the blood type was determined by observation by the naked eye. To evaluate the newtyping kit, we tested sensitivity using artificially diluted blood and compared the results of the new typing method with the slide and tube methods using 70 samples. RESULTS: The new blood typing kit could differentiate a +4~+2 agglutination reaction, but could not detect a +1 agglutination reaction as observed by the naked eye. Among 70 samples, the results of ABO and Rh typing by the new typing method (n=66, > or = +2 agglutination reaction by the column agglutination method) were in accord with the results of the tube and slide methods, but couldnot detect agglutination in all 4 clinical samples, below a +1 agglutination reaction. CONCLUSION: The new blood typing kit is inadequate for routine use in the clinical laboratory due to low sensitivity, but with further improvement, it can be used economically, conveniently and objectively for blood typing without any special equipment. Moreover, the microfludics and separation method may be broadly applicable in other tests using the hemagglutination method.
Agglutination ; Agglutination Tests ; Blood Grouping and Crossmatching* ; Capillary Action ; Hemagglutination ; Immune Sera ; Microfluidics* ; Microtechnology

We report two cass of HIV infection associated with condyeloma acuminatum. Two patients were healthy men who showed multiple pinkish verruc ous papules on the perianal area. Anti-HIV antibodies were detected in the patients' secatory particle agglutination test and confirmed by Western blot assay.
Agglutination Tests ; Antibodies ; Blotting, Western ; HIV Infections ; HIV* ; Humans* ; Male

BACKGROUND: Establishment of a national reference panel for syphilis antibodies is necessary to evaluate the performance of in-vitro diagnostic tests for syphilis and to verify test quality. This study aimed to establish a national reference panel for syphilis antibodies, to assess the suitability of a panel for non-treponemal and treponemal testing, and to assess the reactivity of the various tests currently in use. METHODS: Treponemal pallidum particle agglutination (TPPA)-positive and -negative fresh frozen plasma samples were obtained. After the fresh frozen plasma was converted to serum by defibrination, the samples were pooled. Two candidate reference standards containing no syphilis antibodies and 10 candidate reference standards containing syphilis antibodies were prepared on the basis of reactivity in the TPPA assay. Candidate reference standards were tested by three laboratories using five non-treponemal tests and four treponemal tests. RESULTS: All three laboratories reported positive non-treponemal test results for the mixed-titer performance panel (MP)/6-MP/12. MP/1, MP/2, and MP/3 were negative for non-treponemal tests. MP/4 and MP/5 were reported either as positive or negative according to the laboratories. All laboratories reported positive TPPA results for MP/3-MP/12 and negative results for MP/1 and MP/2. No significant difference was detected among the treponemal testing results in three laboratories. CONCLUSIONS: We established 12 candidate national reference standards containing various concentrations of syphilis antibodies. A collaborative study using nine tests demonstrated that 12 candidate national reference standards presented consistent results, except a few assays with low sensitivity, and thus could be used as a national reference panel for syphilis antibody testing.
Agglutination ; Antibodies* ; Diagnostic Tests, Routine ; Korea* ; Plasma ; Syphilis*