No abstract available.
Aeromonas*

No abstract available.
Aeromonas*

No abstract available.
Aeromonas* ; Enterotoxins*

Aims: This study evaluated the accuracy of three methods used in the identification of Aeromonas hydrophila, a Gramnegative bacterium found in warm aquatic environments. A. hydrophila samples from Indonesia were tested using (a) SNI 7303, developed by the Indonesian government, (b) the method of Dorsch and (c) the method of Cascón. The results obtained were compared to that of the gold standard method, which used 16S rDNA sequences. Methodology and results: Based on the Indonesian government standard identification method SNI7303, we identified 56 out of 95 samples as A. hydrophila. The samples were then screened using the PCR amplification approach developed by Dorsch and Cascón. Of the 56 samples, only 20 samples were found to be positive by either the Dorsch or Cascón methods. DNA from these 20 samples was amplified using common 16S rDNA primers and the sequences compared with available 16S rDNA sequences from the GenBank. Phylogenetic analyses on the data were performed using Clustal X and MEGA 5 software. Conclusion, significance and impact of study: Of the 56 samples positively identified as A. hydrophila using the BSN method, identity in only five samples were positively confirmed using the16S rDNA method, giving an accuracy of only 8.9%. In this connection, the Dorsch method was 31.3% accurate while the Cascón method provided 45.5% correct identification. When all three methods were used in combination, 71.4% of the samples were correctly identified. The results of the study show that methods used to identify A. hydrophila cannot be used with confidence to identify A. hydrophila from Indonesia and probably from other tropical regions as well. The genetic diversity of Aeromonas bacteria in Indonesia appears to be considerably higher than that encountered by Dorsch or Cascón. Therefore, there is a need to develop a new simple method to identify A. hydrophila from tropical regions.
Aeromonas hydrophila

No abstract available.
Aeromonas hydrophila* ; Aeromonas* ; Animals ; Histamine Release* ; Histamine* ; Mast Cells* ; Rats*

Aims: Several harmful dinoflagellate species are widely found in Malaysian marine waters. These microalgae are capable of producing a variety of toxins that can intoxicate humans through consumption of contaminated seafood. Therefore, the present work aimed to investigate the potential of a marine bacterium sdPS-7 isolate as a biological control agent against Alexandrium minutum KB-5. Methodology and results: The isolate sdPS-7 was obtained from marine sediments in Malaysia and examined for its algicidal activity toward the toxic dinoflagellate A. minutum KB-5 in laboratory culture. Based on morphological and molecular characterization, this isolate was identified as Aeromonas hydrophila sdPS-7. Alexandrium minutum KB-5 cultures were exposed to cell free bacteria culture filtrate and the effect on dinoflagelate growth was evaluated based on direct cell counts in the treated samples compared to non-treated control cultures. The bacterial filtrate was treated at different temperatures, salinities and exposed to repeated freezing and thawing five times to study its algicidal activity stability thereafter. The results showed a potent inhibition of the growth of A. minutum KB-5. The strongest effect was observed when the bacterium culture filtrate was mixed with A. minutum KB-5 cultures that were in lag phase, resulting in 99% or higher mortality. The cell-free filtrate proved to be heat- stable when exposed to temperatures of 2 °C to 100 °C for one hour each. There was also no substantial salinity as well as (freezing – thawing) effect on the filtrate algicidal activity. Conclusion, significance and impact of study: This study illustrated the potential use of the marine bacterial Aeromonas hydrophila sdPS-7 filtrates in controlling the growth of the toxic dinoflagellate A. minutum KB-5.
Biological Control Agents ; Aeromonas hydrophila

Aeromonas hydrophila infection has been increasingly found, in particular among patients with various underlying diseases. Many characteristics of this organism are quite similar to those of Enterobacteriaceae and Vibrio, making an accurate identification difficult. In a period of 2 years, the authors obtained a total of 27 isolates of A. hydrophila from clinical materials, and their cultural and biochemical characteristics are herewith reported. Some of the most important clues to suspect this organism were a wide zone of complete hemolysis on blood agar, partially alkaline slant, acid butt, and small amount of gas in trip1e sugar iron agar (TSI), weak indole reaction, and negative ornithine decarboxylase in motility indole ornithine medium (MIO), and usually positive citrate utilization. It is concluded that the identification of this organism should be possible on the basis of deoxyribonuclease (DNase), oxidase, and a few other tests. Our isolates showed a similar antibiotic susceptibility to those reported in other countries; i.e., a11 were resistant to ampicillin and most were susceptible to other antibiotics, excluding cephalothin.
Aeromonas/drug effects ; Aeromonas/isolation & purification* ; Aeromonas/physiology ; Antibiotics/pharmacology ; Culture Media ; Human ; Microbial Sensitivity Tests

To effectively solve the serious impact of high oil in the kitchen wastewater on the downstream treatment process, an excellent oil-degrading strain Aeromonas allosaccarophila CY-01 was immobilized to prepare Chitosan-Aeromonas pellets (CH-CY01) by using chitosan as a carrier. Oil degradation condition and efficiency of CH-CY01 pellets were assessed. The growth of immobilized CH-CY01 was almost unaffected, and the maximum degradation rate of soybean oil was 89.7%. Especially at 0.5% NaCl concentration, oil degradation efficiency of CH-CY01 was increased by 20% compared with free cells. In the presence of a surfactant (sodium dodecylbenzene sulfonate) at 1 mg/L, the degradation efficiency of oil by CH-CY01 was increased by 40%. Moreover, using the high-oil catering wastewater as the substrate, more than 80% of the solid oil was degraded with 1% (V/V) CH-CY01 pellets treatment for 7 days, significantly higher than that of free cells. In summary, immobilized CH-CY01 significantly improved the efficiency of oil degradation.
Aeromonas ; Chitosan ; Surface-Active Agents ; Waste Water

In the present study, Aeromonas (A.) hydrophila was isolated from a captive-bred adult freshwater stingray (Potamotrygon motoro) reared at a commercial aquarium in Korea. The stingray had bites on its fins, hemorrhages on the ventral part, and congested internal organs. A bacterium was isolated from kidney and subsequently identified as A. hydrophila. Based on phylogenetic analysis results, the isolate in the present study (SNUAh-LA1) was most closely related to A. hydrophila AH10 (China) and A. hydrophila AKR1 (Korea). It is most likely that the pathogen infection resulted from Potamotrygon motoro cohabiting with ricefish (Oryzias latipes).
Adult ; Aeromonas hydrophila* ; Aeromonas* ; Estrogens, Conjugated (USP) ; Fresh Water* ; Hemorrhage ; Humans ; Kidney ; Korea

Plesiomonas shigelloides was isolated from blood culture of a 53-year-old man with fever, who had treatment history of gastrointestinal malignancy. The patient showed neither clinical features nor hematological finding which suggest bacteremia. Identification of the isolate was delayed because of its similar characteristics with Aeromonas spp. and other gram-negative bacilli. The isolate was misinterpreted as susceptible to ampicillin by the first disk diffusion test. It may not always easy to identify P. shigelloides by conventional tests and to determine its antimicrobial susceptibility accurately, as laboratorians rarely have experience with the organism and as the organism may show unusual inhibition pattern when tested by disk diffusion method or Etest.
Aeromonas ; Ampicillin ; Bacteremia* ; Diffusion ; Fever ; Humans ; Middle Aged ; Plesiomonas*