1.Recovery and Adsorption Rate of Murine Norovirus Using NanoCeram(R) Filters.
Yun Hee KIM ; Seok Jea JANG ; Ji Youn PARK ; Jung Hwan OH ; Geun Su KIM ; Tae Seung KIM ; Oh Sang KWON ; Jin Seok HAN ; Won Hwa JHEONG
Journal of Bacteriology and Virology 2011;41(1):55-61
This study investigated the recovery and absorption rates of murine norovirus, a surrogate for human norovirus, by using NanoCeram(R) filters which served as a tool for recovering viruses. In the study, two types of NanoCeram(R) filters were employed: one was a cartridge type and the other was a disc type (phi 47 mm) whose surface area is 75 times smaller than the cartridge type. The analytical method was the real-time reverse transcription-polymerase chain reaction (RT-PCR). The study found that the average recovery rates of the cartridge type and the disc type were 30.9% and 29.5% respectively. Since these two rates were very close to each other, the adsorption rate of the cartridge type could be predicted with the disc type. Analyzing recovery and absorption rates of the disc type based on different filtered volumes showed that when the volume increased from 0.5 L to 20 L, the average recovery rate rose from 14.78% to 30.41 %, while the average absorption rate dropped from 56.33% to 10.48%. The increase in turbidity from less than 1 NTU to less than 3 NTU raised the average recovery rate from 47.23% to 82.84%.
Absorption
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Adsorption
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Humans
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Norovirus
2.Transfusion Strategy and Laboratory Update on the DEL Variant.
Min Hee SEO ; Borahm KIM ; Jeong Ran KWON ; Young Sill CHOI ; Jun Nyun KIM ; Kyoung Un PARK ; Duck CHO
Korean Journal of Blood Transfusion 2015;26(1):1-8
Red cells that express extremely low levels of D antigen that cannot be detected by routine serologic tests are designated as DEL. Most DEL blood donors are typed as D-negative. However, DEL red blood cells can be recognized by serological adsorption and elution test or molecular RHD genotyping. Anti-D production in patients with D-negative who received transfusion containing DEL blood has reported, therefore distinction between DEL variant and true D- negative is clinically important. This review highlights a transfusion strategy and laboratory update on the DEL variant in the Korean population.
Adsorption
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Blood Donors
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Erythrocytes
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Humans
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Serologic Tests
3.A comparative study on the agglutination inhibition activities to mouse red blood cells and adsorption activities to human red blood cells of phytagglutinin, caragana chamlagu.
Korean Journal of Legal Medicine 1992;16(1):47-51
No abstract available.
Adsorption*
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Agglutination*
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Animals
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Caragana*
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Erythrocytes*
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Humans*
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Mice*
4.The Tribological and Lubrication Responses of a Cobalt-Chromium Femoral Head in Total Hip Arthroplasty.
Seonghun PARK ; Duong Cong TRUYEN ; Jae Hoon LEE ; Younho CHO ; Seung Jae PARK ; Keun Min PARK ; Jun Dong CHANG ; Sang Soo LEE
Journal of the Korean Hip Society 2010;22(3):227-233
PURPOSE: This study aimed at investigating the role of albumin as a boundary lubricant in the lubrication of the Co-Cr femoral head of artificial hip implants by measuring the tribological parameters of the Co-Cr femoral head with Atomic Force Microscope (AFM) techniques. MATERIALS AND METHODS: Samples were prepared from the main wear region of a Co-Cr femoral head from revision hip surgery. Two types of solutions were prepared as lubricants: PBS (Phosphate Buffered Saline) as a control solution and BSA (Bovine Serum Albumin) as a lubricant at concentrations of 10, 20, 30 and 40 mg/ml in PBS solution. RESULTS: There were statistically significant differences in the frictional coefficients (micron) of a Co-Cr head between the PBS control and all the concentrations of BSA (10, 20, 30, 40 mg/ml) (P<0.001). Similarly, there were statistically significant differences for the micron between the BSA concentrations of 10, 20, 30 and 40 mg/m for all the cases except between the BSA of 30 and 40 mg/ml (P<0.01). CONCLUSION: There exists a maximum protein concentration of BSA to play a role as an effective boundary lubricant through adsorption on the surface of Co-Cr femoral head.
Adsorption
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Arthroplasty
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Friction
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Head
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Hip
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Lubrication
5.Removal of Shield Needles from Graphene Oxide Thrombus and Preparation.
Yuting YANG ; Yuanjian ZHONG ; Lichun ZHAO ; Yuanbo SONG ; Li YU
Chinese Journal of Medical Instrumentation 2021;45(5):492-496
Atherosclerosis is a chronic inflammatory disease commonly seen in clinical practice. It can lead to thickening of vascular intima, occlusion of lumen stenosis and thrombosis, leading to angina pectoris, hypertension, myocardial infarction and other diseases, posing a serious threat to human life and health. This study provides a method for removing shield needles from graphene oxide thrombus and its preparation. The graphene oxide shield needle mainly includes flexible rotating shaft, radial flexible rod, rotating needle, adsorption main pipe and dosing main pipe, laser measuring device, high definition camera and other structures, which has the following advantages:firstly, it achieves multi-angle rotation grinding thrombosis, precise rotation grinding, avoids vascular damage and infection; secondly, thrombolytic drugs can be applied in the process of rotary grinding and small thrombus can be adsorbed to effectively avoid secondary embolization of blood vessels; thirdly, it a coating of graphene oxide on a rotating needle, which protects against bacteria and infection. This study has practical reference value for the development of thrombotherapy and the application of graphene in the medical field.
Adsorption
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Graphite
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Humans
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Needles
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Thrombosis/prevention & control*
6.Ultrafiltration process of model system of Huanglian Jiedu decoction.
Jie DONG ; Huaxu ZHU ; Liwei GUO
China Journal of Chinese Materia Medica 2009;34(19):2458-2462
OBJECTIVEThe UF process of model systerm of Huanglian Jiedu decoction is researched in the study to lay a foundation for the exploring of the substance foudation of membrane fouling, and for the the optimal design of membrane technology in the production of Chinese drugs preparation.
METHODUsing the membrane flux, fouling degree of membrane, preserving degree and adsorption capacity of effective materials as guide line, trying to find out the influence of macromolecule materials on small molecules in Huanglian Jiedu decoction.
RESULTThe two small molecules berberine and gardenoside in Huanglian Jiedu decoction have high permeation rate. But for the reasons of interaction of macromolecule, the permeation rate of small molecules reduced greatly, membrane fouling and membrane flux declined intensify.
CONCLUSIONStarch and pectin are the two main macromolecule materials in Huanglian Jiedu decoction which cause membrane fouling and flux falling.
Adsorption ; Drugs, Chinese Herbal ; chemistry ; Membranes, Artificial ; Ultrafiltration ; instrumentation ; methods
7.Influence of micro-pattern on adherence of graphite powder and three kinds of oral microbes.
Yu-lei QIU ; Xue-dong ZHOU ; Chong-yun BAO
West China Journal of Stomatology 2011;29(3):323-326
OBJECTIVETrying to find out the mechanism of microstructure influencing bacterial adhesion, we prepared different microstructures to compare the adsorptive behavior of graphite powder and adhesive behavior of oral microbe.
METHODSWe used polydimethylsiloxane (PDMS) to copy 23 microstructures of hydroxyapatite (HA) chip, and cultured them with different sizes graphite powder and oral microbes respectively, to observe and compare their behavior on microstructures.
RESULTSThe adsorption of 30-50 microm powder on different microstructures was insignificant, while 10-20 microm powder had maximum adsorption on 10 microm and 20 microm microstructures. Saccharomyces albicans was most likely to adhere to 5 microm microstructures which was equivalent to its own size. However, microstructures had little effect on adhesion of Streptococcus mutans which grew in a chain.
CONCLUSIONThe size of microstructure was the most effective factor that affects the adsorption of non-living powder, and it also had the same effect on the microbial adhesion; but some special bacteria, such as Streptococcus mutans which grew in a chain, was not affected by the sizes or shapes of microstructures.
Adsorption ; Bacteria ; Bacterial Adhesion ; Durapatite ; Graphite ; Mouth ; microbiology ; Streptococcus mutans
8.A Case of BX.
Il Joong PARK ; Young Ae LIM ; Kyou Sup HAN ; Yun Sik KWAK
Korean Journal of Blood Transfusion 1999;10(2):229-232
We report a case of BX in a 27-year-old woman admitted for Cessarian section after diagnosis of placenta previa. An ABO discrepancy was observed, such as, group O in cell typing but group B in serum typing. The patient's red cells were agglutinated by anti-H and show the mixed field agglutination by anti-A,B. The results of adsorption and elution test, saliva test and B transferase test show the presence of B antigen, absence of B substance in saliva and no activity of B-transferase. All of these findings were compatible with BX.
Adsorption
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Adult
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Agglutination
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Diagnosis
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Female
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Humans
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Placenta Previa
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Saliva
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Transferases
9.Using Genotyping to Identify an A1B(weak) Blood Group.
Chi Hyun CHO ; Byong Joon YOO ; Seung Gyu YUN ; Gye Ryung CHOI ; Jae Yeoul CHOI ; Jang Su KIM ; Chae Seung LIM ; Young Kee KIM ; Kap No LEE
Korean Journal of Blood Transfusion 2010;21(2):158-164
Since an exact ABO blood type match is essential for transfusion therapy, any ABO discrepancies should be resolved prior to the issuing of blood. The authors confirmed the ABO blood group of a 50-year-old male using genotyping. On a routine blood group test, the cell type was A+; however, anti-B was undetected in his serum. To determine the cause of this ABO discrepancy, an adsorption elution test and saliva test were performed. The presence of a weak B substance was suspected despite no evidence of the B antigen on red blood cells. Polymerase-chain-reaction restriction-fragment-length-polymorphism (PCR-RFLP) and sequencing analysis of exons 6 and 7 demonstrated that his blood type was A1Bweak (the A allele tested as the A105 subtype, while the B allele was most similar to the B302 subtype). Again, using genotyping, we subsequently confirmed the A1Bweak blood type in a leukemic patient who was in complete remission.
Adsorption
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Alleles
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Erythrocytes
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Exons
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Humans
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Leukemia
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Male
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Middle Aged
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Saliva
10.Adsorption of Salivary Proteins on Titanium Implants.
Seoung Ho LEE ; Young KU ; Yong Moo LEE ; In Cheol RHYU ; Chong Pyoung CHUNG ; Soo Boo HAN ; Sang Mook CHOI
The Journal of the Korean Academy of Periodontology 2003;33(2):127-137
No abstract available.
Adsorption*
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Dental Pellicle
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Saliva
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Salivary Proteins and Peptides*
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Titanium*