1.Recovery and Adsorption Rate of Murine Norovirus Using NanoCeram(R) Filters.
Yun Hee KIM ; Seok Jea JANG ; Ji Youn PARK ; Jung Hwan OH ; Geun Su KIM ; Tae Seung KIM ; Oh Sang KWON ; Jin Seok HAN ; Won Hwa JHEONG
Journal of Bacteriology and Virology 2011;41(1):55-61
This study investigated the recovery and absorption rates of murine norovirus, a surrogate for human norovirus, by using NanoCeram(R) filters which served as a tool for recovering viruses. In the study, two types of NanoCeram(R) filters were employed: one was a cartridge type and the other was a disc type (phi 47 mm) whose surface area is 75 times smaller than the cartridge type. The analytical method was the real-time reverse transcription-polymerase chain reaction (RT-PCR). The study found that the average recovery rates of the cartridge type and the disc type were 30.9% and 29.5% respectively. Since these two rates were very close to each other, the adsorption rate of the cartridge type could be predicted with the disc type. Analyzing recovery and absorption rates of the disc type based on different filtered volumes showed that when the volume increased from 0.5 L to 20 L, the average recovery rate rose from 14.78% to 30.41 %, while the average absorption rate dropped from 56.33% to 10.48%. The increase in turbidity from less than 1 NTU to less than 3 NTU raised the average recovery rate from 47.23% to 82.84%.
Absorption
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Adsorption
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Humans
;
Norovirus
2.Transfusion Strategy and Laboratory Update on the DEL Variant.
Min Hee SEO ; Borahm KIM ; Jeong Ran KWON ; Young Sill CHOI ; Jun Nyun KIM ; Kyoung Un PARK ; Duck CHO
Korean Journal of Blood Transfusion 2015;26(1):1-8
Red cells that express extremely low levels of D antigen that cannot be detected by routine serologic tests are designated as DEL. Most DEL blood donors are typed as D-negative. However, DEL red blood cells can be recognized by serological adsorption and elution test or molecular RHD genotyping. Anti-D production in patients with D-negative who received transfusion containing DEL blood has reported, therefore distinction between DEL variant and true D- negative is clinically important. This review highlights a transfusion strategy and laboratory update on the DEL variant in the Korean population.
Adsorption
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Blood Donors
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Erythrocytes
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Humans
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Serologic Tests
3.A comparative study on the agglutination inhibition activities to mouse red blood cells and adsorption activities to human red blood cells of phytagglutinin, caragana chamlagu.
Korean Journal of Legal Medicine 1992;16(1):47-51
No abstract available.
Adsorption*
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Agglutination*
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Animals
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Caragana*
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Erythrocytes*
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Humans*
;
Mice*
4.The Tribological and Lubrication Responses of a Cobalt-Chromium Femoral Head in Total Hip Arthroplasty.
Seonghun PARK ; Duong Cong TRUYEN ; Jae Hoon LEE ; Younho CHO ; Seung Jae PARK ; Keun Min PARK ; Jun Dong CHANG ; Sang Soo LEE
Journal of the Korean Hip Society 2010;22(3):227-233
PURPOSE: This study aimed at investigating the role of albumin as a boundary lubricant in the lubrication of the Co-Cr femoral head of artificial hip implants by measuring the tribological parameters of the Co-Cr femoral head with Atomic Force Microscope (AFM) techniques. MATERIALS AND METHODS: Samples were prepared from the main wear region of a Co-Cr femoral head from revision hip surgery. Two types of solutions were prepared as lubricants: PBS (Phosphate Buffered Saline) as a control solution and BSA (Bovine Serum Albumin) as a lubricant at concentrations of 10, 20, 30 and 40 mg/ml in PBS solution. RESULTS: There were statistically significant differences in the frictional coefficients (micron) of a Co-Cr head between the PBS control and all the concentrations of BSA (10, 20, 30, 40 mg/ml) (P<0.001). Similarly, there were statistically significant differences for the micron between the BSA concentrations of 10, 20, 30 and 40 mg/m for all the cases except between the BSA of 30 and 40 mg/ml (P<0.01). CONCLUSION: There exists a maximum protein concentration of BSA to play a role as an effective boundary lubricant through adsorption on the surface of Co-Cr femoral head.
Adsorption
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Arthroplasty
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Friction
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Head
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Hip
;
Lubrication
5.Removal of Shield Needles from Graphene Oxide Thrombus and Preparation.
Yuting YANG ; Yuanjian ZHONG ; Lichun ZHAO ; Yuanbo SONG ; Li YU
Chinese Journal of Medical Instrumentation 2021;45(5):492-496
Atherosclerosis is a chronic inflammatory disease commonly seen in clinical practice. It can lead to thickening of vascular intima, occlusion of lumen stenosis and thrombosis, leading to angina pectoris, hypertension, myocardial infarction and other diseases, posing a serious threat to human life and health. This study provides a method for removing shield needles from graphene oxide thrombus and its preparation. The graphene oxide shield needle mainly includes flexible rotating shaft, radial flexible rod, rotating needle, adsorption main pipe and dosing main pipe, laser measuring device, high definition camera and other structures, which has the following advantages:firstly, it achieves multi-angle rotation grinding thrombosis, precise rotation grinding, avoids vascular damage and infection; secondly, thrombolytic drugs can be applied in the process of rotary grinding and small thrombus can be adsorbed to effectively avoid secondary embolization of blood vessels; thirdly, it a coating of graphene oxide on a rotating needle, which protects against bacteria and infection. This study has practical reference value for the development of thrombotherapy and the application of graphene in the medical field.
Adsorption
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Graphite
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Humans
;
Needles
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Thrombosis/prevention & control*
6.Unfolded Histidine-Tagged Protein is Immobilized to Nitrilotriacetic Acid-Nickel Beads, But Not the Nickel-Coated Glass Slide.
Minho CHO ; Sunyoung AHN ; Heonyong PARK
Genomics & Informatics 2006;4(3):133-136
The adsorption of proteins on the surface of glass slides is essential for construction of protein chips. Previously, we prepared a nickel-coated plate by the spin-coating method for immobilization of His-tagged proteins. In order to know whether the structural factor is responsible for the immobilization of His-tagged proteins to the nickel-coated glass slide, we executed a series of experiments. First we purified a His-tagged protein after expressing the vector in E. coli BL21 (DE3). Then we obtained the unfolding curve for the His-tagged protein by using guanidine hydrochloride. Fractions unfolded were monitored by internal fluorescence spectroscopy. The delta G(H20) for unfolding was 2.27 kcalmol +/- 0.52. Then we tested if unfolded His-tagged proteins can be adsorbed to the nickel-coated plate, comparing with Ni2+ -NTA (nitrilotriacetic acid) beads. Whereas unfolded His-tagged proteins were adsorbed to Ni2+ -NTA beads, they did not bind to the nickel-coated plate. In conclusion, a structural factor is likely to be an important factor for constructing the protein chips, when His-tagged proteins will immobilize to the nickel-coated slides.
Adsorption
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Fibrinogen
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Glass*
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Guanidine
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Immobilization
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Protein Array Analysis
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Spectrometry, Fluorescence
7.Development of decision support system for antibody identification.
Kyung Hwan CHOI ; Kyou Sup HAN ; Bok Yeon HAN ; Jin Tae SUH ; Suhng Gwon KIM ; Han Ik CHO
Korean Journal of Blood Transfusion 1998;9(2):167-173
BACKGROUND: Determination of antibody specificity using antigram spread sheet requires experience and knowledge on in vitro characteristics of red cell antibodies, time-consuming, and still subjective to human error. A computer-based antibody identification system was developed to overcome these disadvantages. METHODS: Decision support system program for antibody identification was designed using Visual Basic 5.0 for Dade Data-cyte Plus. This system integrates the reaction patterns of saline, 37degrees C albumin, antiglobulin, 4degrees C saline enzyme treated and user-defined phases and lists the antibodies according to the probability. 115 irregular antibodies previously confirmed by standard manual method reanalyzed with this program. RESULTS: In 111 of 115 cases (96.5%), this system produced the same results with the manual identification. In two cases, of not matched 4 cases the computer program suggested additional antibodies and in one case, the computer program detected previous human error. In the other case, antibody identification was possible only after further tests including selective adsorption of multiple antibodies. CONCLUSION: The decision support system was rapid and easy and showed good concordance rate when compared with manual antibody identificaion results. In addition, human error could be reduced. Decision support system for antibody identification could be used in small blood banks by less experienced staffs.
Adsorption
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Antibodies
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Antibody Specificity
;
Blood Banks
;
Expert Systems
;
Humans
8.Effects of various surface treatments for titanium on surface micro roughness, static wettability, fibronectin adsorption.
Hwa Sub SHIN ; Young Su KIM ; Sang Wan SHIN
The Journal of Korean Academy of Prosthodontics 2006;44(4):443-454
Purpose: This study aims to get the fundamental data which is necessary to the development direction of implant surface treatment hereafter, based on the understanding the surface structure and properties of titanium which is suitable for the absorption of initial tissue fluid by researching effects of additional surface treatments for sandblasted with large grit and acid-etched(SLA) titanium on surface micro-roughness, static wettability, fibronectin adsorption. Materials and Method: In the Control groups, the commercial pure titanium disks which is 10mm in diameter and 2mm in thickness were treated with HCI after sandblasting with 50micrometer Al2O3. The experiment groups were made an experiment each by being treated with 1) 22.5% nitric acid according to SLA+ASTM F86 protocol, 2) SLA+30% peroxide, 3) SLA+NaOH, 4) SLA+Oxalic acid, and 5) SLA+600degree C heating. In each group, the value of Ra and RMS which are the gauges of surface roughness was measured, surface wettability was measured by analyzing with Sessile drop method, and fibronectin adsorption was measured with immunological assay. The significance of each group was verified by (SPSS, ver.10.0 SPSS Inc.) Kruskal-Wallis Test.(alpha=0.05) And the correlation significance between Surface micro-roughness and surface wettability, surface roughness and fibronectin adsorption, and surface wettability and fibronectin adsorption was tested by Spearman's correlation analysis. Result: All measure groups showed the significant differences in surface micro-roughness, surface wettability, and fibronectin adsorption.(p<0.05) There was no significance in correlation among the surface micro-roughness, surface wettability, and fibronectin adsorption.(p>0.05) Conclusion: Surface micro-roughness and surface wettability rarely affected the absorption of initial tissue fluid on the surface of titanium.
Absorption
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Adsorption*
;
Fibronectins*
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Heating
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Hot Temperature
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Nitric Acid
;
Titanium*
;
Wettability*
9.A Study of Surface Physical Properties of New Surfactant Using Synthetic Peptides of Surfactant Protein-B.
Chong Woo BAE ; Yong Mook CHOI ; Joo Hyun KANG ; Sang Chul LEE ; Kil Lyong KIM ; Kyung Soo HAHM
Journal of the Korean Pediatric Society 2000;43(8):1059-1067
PURPOSE: To produce a new generation of artificial pulmonary surfactant(PS), surfactant protein (SP)-B from human PSwas isolated, and the amino acid sequences of these proteins were studied. Artificial peptides of human SP-B were synthesized. New artificial PS preparations which were cornposed of phospholopids and two artificial synthetic SP-B peptides were made, and the surface physical properties of these new PS preparations were tested. METHODS: The purities of SP-B were assessed by SDS-polyacrylamide gel and the amino acid sequences of these proteins were determined. We synthetized two peptides SP-1 and SP-2 and the amino acid sequences were as follows,' SP-1: RMLPQLVCRLVLRCSMD, SP-2: RMLP- QLVCRLVLRCSM. Surface physical properties of newly artificial PSs, which were composed of a mixture of phospholipid(PL) and SP-1 or SP-2(sample A; PL+SP-1, sample B; PL+SP-2), were measured by surface spreading, adsorption rate, and surface tension-area diagram. RESULTS: The amino acid sequence of human SP-B was obtained. We produced the artificial peptides of SP-B and prepared the new generation PS(sample A and sample B). The order of the superiority of spreading and adsorption rate was Surfacten
10.Adsorption of Salivary Proteins on Titanium Implants.
Seoung Ho LEE ; Young KU ; Yong Moo LEE ; In Cheol RHYU ; Chong Pyoung CHUNG ; Soo Boo HAN ; Sang Mook CHOI
The Journal of the Korean Academy of Periodontology 2003;33(2):127-137
No abstract available.
Adsorption*
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Dental Pellicle
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Saliva
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Salivary Proteins and Peptides*
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Titanium*