Adipose Tissue ; transplantation ; Humans ; Mammaplasty ; methods ; Safety ; Transplantation, Autologous

OBJECTIVEClinical application of autologous fat grafting (AFG) is quickly expanding. Despite the widely acceptance, long-term survival rate (SR) of AFG remains a question not yet solved. Meanwhile, although rare, severe complications related to AFG including vision loss, stroke even death could be seen in the literature.

DATA SOURCESA comprehensive research of PubMed database to June 2013 was performed according to guidelines of the American Society of Plastic Surgeons Fat Graft Task Force Assessment Methodology. Articles were screened using predetermined inclusion and exclusion criteria.

STUDY SELECTIONData collected included patient characteristics, surgical technique, donor site, recipient site, graft amount, and quantified measurement methods. Patient cohorts were pooled, and SR was calculated. All the severe complications were also summarized according to the different clinical characteristics.

RESULTSOf 550 articles, 16 clinical articles and 10 animal studies met the inclusion criteria and provided quantified measurement methods. Totally, 596 patients were included. SR varied from 34% to 82% in breast and 30-83% in the facial area. Nude mice were applied to investigate human fat grafting SR (38.3-52.5% after 15 weeks). Rabbits were commonly used to study animal AFG SR (14.00-14.56% after 1-year). Totally, 21 severe complications were reported, including death (2), stroke (10), vision loss (11, 8 of which accompanied with stroke), sepsis (3), multiple abscess (1) and giant fat necrotic cyst (2). Ten of these complications happened within 10 years.

CONCLUSIONSThere is no unified measurement method to evaluate fat graft SR until now and no clinical evidence to show better SR according to different donor and recipient cite. Body mass index change between pre- and postoperation may be the bias factor in evaluating fat SR. Fat embolisms of the ophthalmic artery and the middle cerebral artery are the most severe complication of AFG and still lack of effective treatment.

Adipose Tissue ; transplantation ; Animals ; Autografts ; Humans ; Survival Rate

BACKGROUND: Fat grafting, or lipofilling, represent frequent clinically used entities. The fate of these transplants is still not predictable, whereas only few animal models are available for further research. Quantum dots (QDs) are semiconductor nanocrystals which can be conveniently tracked in vivo due to photoluminescence. METHODS: Fat grafts in cluster form were labeled with cadmium-telluride (CdTe)-QD 770 and transplanted subcutaneously in a murine in vivo model. Photoluminescence levels were serially followed in vivo. RESULTS: Tracing of fat grafts was possible for 50 days with CdTe-QD 770. The remaining photoluminescence was 4.9%±2.5% for the QDs marked fat grafts after 30 days and 4.2%± 1.7% after 50 days. There was no significant correlation in the relative course of the tracking signal, when vital fat transplants were compared to non-vital graft controls. CONCLUSIONS: For the first-time fat grafts were tracked in vivo with CdTe-QDs. CdTe-QDs could offer a new option for in vivo tracking of fat grafts for at least 50 days, but do not document vitality of the grafts.
Adipocytes ; Adipose Tissue* ; Models, Animal ; Quantum Dots* ; Transplantation ; Transplants*

This article summarizes methods of repair of massive and deep wounds, elucidates how to improve wound healing quality and avoid scar deformity after deep burn. A part of denatured dermis (non-necrotic) in deep partial-thickness burn, "mixed degree" burn, even in full-thickness burn wounds before forming eschar can be preserved and covered with autologous skin, thereby to avoid secondary damage to the structure of subcutaneous tissue and the junction of dermis-adipose, thus to result in good functions, appearance, and survival rate. After skin grafting, wound healing quality and appearance are improved, joint function and elasticity of skin are enhanced, the degree of scar contracture is relieved due to preservation of normal adipose tissue after escharectomy. The study of composite artificial skin will be actively developed in the future. Tissue-engineering skin and stem cells can be successfully used in patients with deep burns for scarless healing with restoration of physiological functions in a short period.
Adipose Tissue ; transplantation ; Burns ; surgery ; therapy ; Humans ; Reconstructive Surgical Procedures ; Skin Transplantation ; Tissue Engineering ; Wound Healing

Regenerative medicine is an emerging discipline. Adipose tissue is a rich source of fat cells and mesenchymal stem cells, and autologous fat grafting has increasingly been applied in plastic surgeries and dermatological treatments. This paper reviews the latest advances in autologous fat grafting in scar revision.
Adipocytes ; transplantation ; Adipose Tissue ; cytology ; Cicatrix ; surgery ; Humans ; Mesenchymal Stem Cell Transplantation ; Reconstructive Surgical Procedures

OBJECTIVETo observe the effects of the fat flap tissues after delay operation on free fat-graft survival rate and duration.

METHODSThe delay operation of fat flaps was performed in the inguinal region of a rabbit. Expression of VEGF was assayed using Elisa method after 12 hours of flap delay. The fat flaps were harvested and cut into pieces after 21 days. A subdermal pocket was created in each side of the dorsal midline of a rabbit, the fat pieces were grafted randomly into a pocket and the normal fat pieces into the other pocket as control. After 1, 3, 6, 9 and 12 months of implantation, the grafted fats were harvested, gross observation, weight measurement and histology were carried out. Number of the vessels stained with anti-CD34 antibody was counted out.

RESULTSVEGF concentrations in flaps were significantly higher (P < 0.05). The density of vessels in experimental groups increased significantly compared with that in control groups at 1 and 3 months, respectively (P < 0.01), and no significant differences in the survival rate of fat tissues between experimental and control groups were observed at 1 and 3 months (P > 0.05). The fat cells from the flaps survived after 12 months of fat plantation, while those in control groups disappeared after 6 months.

CONCLUSIONSThe survival rate and duration of grafted fat could be increased implanting the fat tissues from delayed fat flap, which may provide researchers with a new method for fat graft.

Adipocytes ; transplantation ; Adipose Tissue ; transplantation ; Animals ; Graft Survival ; Male ; Rabbits ; Surgical Flaps

OBJECTIVETo observe the effects of autologous fat granules in mixed grafting microskin grafts on repair of extensive deep burn wounds in patients.

METHODSTwenty patients hospitalized in our ward were enrolled for autogenous self-control test in wounds on both or symmetrical parts of wounds of the trunk, and they were randomly divided into experimental (E) trol (C) groups, the wounds in E group were repaired with autologous fat granules together with microskin in mixed grafting (volume ratio 1 : 1), and in C group only autologous microskin grafting was given. Wound healing rate was measured on 30th, 45th, and 60th day after operation. Wound specimens harvested for HE staining and PCNA immunohistochemistry examination on 7th, 14th, 21st, and after operation.

RESULTS(1) The mean wound healing rate on 30th, 45th, and 60th day after E group was (56.3 +/- 3.1)%, (76.4 +/-6.1)%, (96.2 +/- 1.5)%, which were respectively higher C group [(28.3 +/-2.0)%, (47.3 +/-4.8)%, (85.4 +/- 2.2)%, P < 0.01]. HE staining showed epithelization in E group was earlier than that in C group, with regular arrangement of collagen fibers. The quantity NA positive cells in E group were larger than that in C group, and PCNA was mainly expressed cells of basal layer .

CONCLUSIONAutologous fat granules in mixed grafting with autologous microskin promote wound healing.

Adipose Tissue ; transplantation ; Adult ; Burns ; surgery ; Female ; Humans ; Male ; Skin Transplantation ; methods ; Transplantation, Autologous ; Transplantation, Homologous ; Wound Healing

OBJECTIVETo investigate the function of basic fibroblastic growth factor on the survival of fat transplantation.

METHODSBasic fibroblastic growth factor was used in pearl fat graft transplantation on experimental animal models. Microvessels densities both on experimental sides and control sides were quantitatively researched in various periods. The growth course of vessels was observed.

RESULTSMicrovessels can be observed clearly. The Microvessels densities both on experimental sides and control sides raised gradually. The density reached highest in 14 days on experimental side and in 28 days on control side, and fell down slightly later. The densities on every experimental sides were higher than that on control sides.

CONCLUSIONSBasic fibroblastic growth factor can effectually accelerate the growth of blood vessels in pearl fat graft.

Adipose Tissue ; transplantation ; Animals ; Fibroblast Growth Factor 2 ; therapeutic use ; Neovascularization, Physiologic ; physiology ; Rats ; Rats, Sprague-Dawley ; Tissue Transplantation ; methods

OBJECTIVETo establish a new method to accurately measure the viability of fat for grafting, which can lay a foundation for the further study of fat transplantation.

METHODSWe centrifugated 5 groups of fat respectively at 1000 r/min, 2000 r/min, 3000 r/min, 4000 r/min and 5000 r/min, took out 12 samples from per group of centrifugated fat, 5 ml per sample, and put them into dishes to incubate with DMEM containing glucose for one hour. Simultaneously we set up 5 control dishes (only containing DMEM without fat) to incubate together with those experimental dishes. After incubating we measured the glucose consistency of DMEM in all dishes. The glucose consistency difference between the mean value of 5 control dishes and per experimental dish represented the glucose transportation quantity of per sample, in other words, the sample viability. We analyzed and compared the glucose transportation quantity of every group of samples with t test. The 5 groups of fat were also put in histopathologic examination and the amounts of intact adipocytes on per group of slides were compared with T test.

RESULTSThe glucose transportation quantities of group 1000 r/min, 2000 r/min, 3000 r/min, 4000 r/min, 5000 r/min gradually decreased in turn (P < 0.05). The histopathologic examination didn't show the difference among group 1000 r/min, 2000 r/min, 3000 r/min and 4000 r/min the 5000 r/min (P > 0.05), and only showed the amounts of intact adipocytes on slides of group 5000 r/min were significantly fewer than other four group (P < 0.05).

CONCLUSIONThe glucose transportation test can be used to accurately measure the viability of fat. The histopathologic examination can reveal fat viability roundly.

Adipose Tissue ; pathology ; transplantation ; Adult ; Female ; Glucose ; analysis ; Humans ; Pathology, Clinical ; methods

Facial beauty depends on the form, proportion and position of its various units. The chin is the most prominent element of the lower third of the face, both in the frontal view and in profile. Whether it is advisable to perform rhinoplasty first, followed by genioplasty, or the reverse depends on the type and severity of the deformities of the nose and chin. The selection of material is a matter of the plastic surgeon's preference. Fat is an ideal tissue filler substance because it is living tissue and from the patient's own body. So it is nonallergenic. The procedure can be performed alone as a chin augmentation with fat grafting or in combination with rhinoplasty or other facial surgery. The technique requires minimally traumatic fat harvesting, fat preparation, and multi-level facial infiltration. Remnant fat in the initial procedure is frozen and stored at -18C and can be used successfully for minor touch-up procedures. We have performed a review of chin surgery, the multiple aesthetic analyses available and the advantages and disadvantages of the various materials. Autologous fat transfer to the chin is safe, cost effective, and can produce long term aesthetic improvement. Although there are many synthetic fillers available, autologous fat is perhaps the best option for genioplasty. This simple, fast procedure is a very good alternative for patients with some form of microgenia or when patients and surgeons are not likely to use alloplastic implants.
Adipose Tissue ; Beauty ; Chin ; Congenital Abnormalities ; Genioplasty ; Humans ; Mandible ; Nose ; Rhinoplasty ; Transplantation, Autologous ; Transplants