1.Expression of CysLTR-1 and CysLTR-2 in adenoid tissues from children with adenoid hypertrophy.
Mei-Hua ZHU ; Min LIANG ; Zhi-Jian WANG ; Yan SHEN ; Hong-Yan WEN ; Jin-Ming ZHAI ; Xiao-Bin YANG
Chinese Journal of Contemporary Pediatrics 2015;17(2):159-163
OBJECTIVETo examine the expression of cysteinyl leukotriene receptor-1 (CysLTR-1) and cysteinyl leukotriene receptor-2 (CysLTR-2) in the adenoid tissues from children with adenoid hypertrophy (AH) and to explore the role of leukotrienes in the pathogenesis of AH.
METHODSSixty children with AH who were treated by adenoidectomy and/or tonsillectomy were classified into two groups: simple AH and AH plus allergic rhinitis (n=30 each). Twenty children who underwent tonsillectomy due to recurrent purulent tonsillitis were selected as the control group. The expression of CysLTR-1 and CysLTR-2 in the excised tonsil and/or adenoid tissues was determined by immunofluorescence histochemical labeling and integrated optical density measurement.
RESULTSThe expression of CysLTR-1 and CysLTR-2 in the adenoid and tonsil tissues increased significantly in both the simple AH group and AH plus allergic rhinitis group compared with the control group (P<0.01). The expression of CysLTR-1 and CysLTR-2 in the AH plus allergic rhinitis group increased more significantly compared with the simple AH group (P<0.01).
CONCLUSIONSCysLTR-1 and CysLTR-2 are highly expressed in the adenoid tissues from children with AH, suggesting that leukotrienes are involved in the pathogenesis of AH.
Adenoids ; chemistry ; pathology ; Adolescent ; Child ; Child, Preschool ; Female ; Fluorescent Antibody Technique ; Humans ; Hypertrophy ; Male ; Receptors, Leukotriene ; analysis ; physiology ; Rhinitis, Allergic ; metabolism
2.Squamous Metaplasia and BCL-6 in Pediatric Adenoid Accompanied by Otitis Media with Effusion.
Seung Geun YEO ; Dong Choon PARK ; Jae Hak CHOO ; Chang Il CHA
Yonsei Medical Journal 2007;48(3):449-456
PURPOSE: Deterioration of local immunity in the adenoids may make them vulnerable to infection by microorganisms, resulting in otitis media with effusion. To determine the factors associated with this condition, we evaluated adenoid size, mucosal barrier, squamous changes of ciliated epithelium, IgA secretion, and BCL-6 expression in adenoids. MATERIALS AND METHODS: Seventeen children diagnosed with otitis media with effusion (OME group) and 20 children without any history of OME (control group) were enrolled. Their adenoids were sized by lateral view X-ray and stained with hematoxylin and eosin to detect squamous metaplasia. The adenoids were also stained with cytokeratin to evaluate mucosal barriers, and with anti- IgA antibody and anti- BCL-6 antibody to determine expression of IgA and BCL-6. RESULTS: The OME group showed greater incidence of squamous metaplasia, fewer ciliated cells, and lower expression of BCL-6 (p < 0.05 each). Deterioration of the mucosal barrier was detected in the OME group (p > 0.05). IgA secretion and adenoid size were the same for the OME and the control groups. CONCLUSION: These results suggest that increased squamous metaplasia and lower BCL-6 expression in adenoids may be associated with increased susceptibility to OME.
Adenoids/chemistry/*pathology
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Child
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Child, Preschool
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Female
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Humans
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Immunoglobulin A/analysis
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Immunohistochemistry
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Keratins/analysis
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Male
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Metaplasia
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Mucous Membrane/chemistry/pathology
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Otitis Media with Effusion/metabolism/*pathology
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Proto-Oncogene Proteins c-bcl-6/*analysis