1.Helicobacter pylori infection and changes of cell gap junction of gastric epithelial cells in patients with gastric cancer and precancerous lesion.
Can-xia XU ; Yan JIA ; Wen-bin YANG ; Hui-fang ZOU ; Fen WANG ; Shou-rong SHEN
Journal of Central South University(Medical Sciences) 2008;33(4):338-343
OBJECTIVE:
To observe the changes of cell gap junction ultrastructure of gastric epithelial cells in patients with gastric cancer(GC) and precancerous lesion(PL),and to investigate the relation between these changes and H.pylori infection.
METHODS:
Seventy patients with GC, 88 with PL, and 33 with chronic superfial gastritis (CSG) were studied. H.pylori was detected by rapid urease test,basic fuchsin stain and 14C-urea breath test. The CagA gene of H.pylori was determined by polymerase chain reaction(PCR).The cell gap junction ultrastructure was observed under transmission electronic microscope.
RESULTS:
Length of junction/unit perimeter of gastric epithelial cells in patients with PL was smaller than that in CSG patients, and the smallest width of the intercellular space was bigger than that in CSG patients. The number of cell junction, the number of junction/unit perimeter, and the length of junction/unit perimeter in patients with GC were all smaller than those in patients with CSG or PL, and its smallest width of the intercellular space was bigger than that in patients with CSG. In patients with GC, the number of cell junction, the number of junction/unit perimeter and the length of junction/unit perimeter in CagA+ H.pylori group were smaller than those in CagA(-) H.pylori group, and its smallest width of the intercellular space was bigger than that in CagA(-) H.pylori group. In PL patients, the intercellular space decreased, and the length of cell junction of gastric epithelial cells became bigger after H.pylori eradication. The length of junction/unit perimeter in patients of H.pylori eradication was bigger than that in patients without eradication, and the smallest width of the intercellular space was smaller than that in patients without eradication.
CONCLUSION
The changes of cell gap junction of gastric epithelial cells in patients with GC and PL are associated with H.pylori infection especially CagA+ H.pylori infection. Eradication of H.pylori can promote the formation of cell junction.
Adenocarcinoma
;
microbiology
;
ultrastructure
;
Epithelial Cells
;
ultrastructure
;
Female
;
Gastric Mucosa
;
ultrastructure
;
Helicobacter Infections
;
pathology
;
Helicobacter pylori
;
Humans
;
Intercellular Junctions
;
ultrastructure
;
Male
;
Precancerous Conditions
;
microbiology
;
ultrastructure
;
Stomach Neoplasms
;
microbiology
;
ultrastructure
2.Grading of Histology, Expression of Apoptosis and Cell Proliferation in Gastric Mucosa Adjacent to Gastric Adenoma or Adenocarcinoma.
Jin Tae JUNG ; Chang Hyeong LEE ; Sung Soo YOU ; Hyung Keun HA ; Jong Seok BAE ; Joong Goo KWON ; Eun Young KIM ; Ho Gak KIM ; Chang Ho CHO ; Im Hee SHIN
The Korean Journal of Gastroenterology 2005;46(4):269-275
BACKGROUND/AIMS: Helicobacter pylori (H. pylori) infection can lead to gastric adenoma and carcinoma through atrophic gastritis and intestinal metaplasia. Imbalance between apoptosis and proliferation may play a role in gastric carcinogenesis. We tried to investigate H. pylori infection rate, grade of gastritis, environmental risk factors, expression rate of apoptosis and cell proliferation in mucosa adjacent to tumor, and we also tried to find significant factors associated with gastric carcinogenesis. METHODS: Endoscopically diagnosed twenty cases of intestinal type gastric carcinoma, 20 cases of gastric adenoma, and 40 cases of control (normal or gastritis) were enrolled. H. pylori infection rate, histologic grading, apoptosis and immunohistochemical stain (Ki-67 and p53) to check mucosal proliferation were done in endoscopically biopsied tissues at antrum and body at least 2 cm apart from adenoma or carcinoma. RESULTS: In three groups, H. pylori infection rates were not significantly different. In the multivariate analysis, only atrophy of gland was a significant risk factor for adenoma compared to control group (OR 3.7). Intestinal metaplasia in antrum and alcohol drinking were significant risk factors for carcinoma compared to control group (OR 4.4 and 4.9 respectively). Expressions of apoptosis, Ki-67 and p53 were not significantly different in three groups. CONCLUSIONS: Intestinal metaplasia in antrum and alcohol drinking are significant risk factors for gastric carcinoma. Degree of mucosal proliferation and apoptosis in gastric mucosa adjacent to tumor are not significantly different in three groups.
Adenocarcinoma/microbiology/*pathology
;
Adenoma/microbiology/*pathology
;
Adult
;
Aged
;
Aged, 80 and over
;
*Apoptosis
;
*Cell Proliferation
;
English Abstract
;
Female
;
Gastric Mucosa/*pathology
;
Gastritis/microbiology/pathology
;
Helicobacter Infections/complications/pathology
;
Helicobacter pylori
;
Humans
;
Male
;
Middle Aged
;
Risk Factors
;
Stomach Neoplasms/microbiology/*pathology
3.Gastric Syphilis Mimicking Adenocarcinoma: A Case Report.
Yoon La CHOI ; Jae Joon HAN ; Da Keun LEE ; Min Ho CHO ; Ghee Young KWON ; Young Hyeh KO ; Cheol Keun PARK ; Geunghwan AHN
Journal of Korean Medical Science 2006;21(3):559-562
Syphilis is an unexpected diagnosis in the stomach, and the reduced incidence of syphilis has made its clinical presentation less widely appreciated. We report a 43-yr-old man suffering from epigastric tenderness with an initial diagnosis of gastric carcinoma; gastric syphilis was confirmed by demonstrating spirochetes in a gastric biopsy specimen by silver impregnation. Excessive lymphoplasmacytic infiltration with diffuse thickening of gastric rugae should raise suspicion of gastric syphilis, which should be considered in the differential diagnosis of diffuse erosive gastritis and infiltrative lesions of the stomach.
Syphilis/*diagnosis
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Stomach Neoplasms/*diagnosis
;
Stomach Diseases/*diagnosis/microbiology
;
Stomach/microbiology
;
Male
;
Humans
;
Fluorescent Treponemal Antibody-Absorption Test
;
Enzyme-Linked Immunosorbent Assay
;
*Diagnosis, Differential
;
Biopsy
;
Adult
;
Adenocarcinoma/*diagnosis
4.The Role of Interleukin-1beta Gene Polymorphism in the Gastric Carcinogenesis.
Won Kyung KANG ; Won Sang PARK ; Hyung Min CHIN ; Cho Hyun PARK
The Korean Journal of Gastroenterology 2004;44(1):25-33
BACKGROUND/AIMS: This study was aimed to investigate the polymorphism of interleukin-1beta(IL-1B) and IL-1 receptor antagonist (IL-1RN) gene and the relationship between genotypes and development of gastric adenocarcinoma in Korean, and to investigate the role of Helicobactor pylori (H. pylori) infection. METHODS: The study population comprised of 258 patients with gastric adenocarcinoma. They were classified according to Lauren's classification and the status of H. pylori infection. Genomic DNA was extracted from the gastric tissue. As a control, genomic DNA from peripheral lymphocyte of 100 healthy individuals was used. The amplified products of -511 bp and -31 bp fragments in the IL-1B by PCR were digested by restriction enzyme and separated for RFLP. Variable number tandem repeats were amplified and subjected to RFLP of IL-1RN. RESULTS: There was no significant difference in the genotype of IL-1B-511T and IL-1B-31C between the adenocarcinoma group and the control group. IL-1RN allele 1 homozygote in the intestinal type showed high frequency of 91.7% (p=0.007). In the H. pylori-positive group of the adenocarcinoma, the frequency of IL-1B-31C was significantly higher than that of H. pylori-negative group (p=0.045). CONCLUSIONS: The single nucleotide polymorphism of IL-1B-31C may contribute to the development of the gastric adenocarcinoma in the H. pylori-positive population.
Adenocarcinoma/*genetics/microbiology
;
Aged
;
English Abstract
;
Female
;
Helicobacter Infections/complications
;
Helicobacter pylori
;
Humans
;
Interleukin-1/*genetics
;
Male
;
Middle Aged
;
*Polymorphism, Single Nucleotide
;
Stomach Neoplasms/*genetics/microbiology
5.Detection of Helicobacter DNA in bile from bile duct diseases.
Im Hwan ROE ; Jung Taik KIM ; Hak Sung LEE ; Jong Hwa LEE
Journal of Korean Medical Science 1999;14(2):182-186
Several species of Helicobacter colonize the hepatobiliary tract of animals and cause hepatobiliary diseases. The aim of this study is to investigate Helicobacter found in the biliary tract diseases of humans. Thirty-two bile samples (15 from bile duct cancer, 6 from pancreatic head cancer, and 11 from intrahepatic duct stone) were obtained by percutaneous transhepatic biliary drainage. Polymerase chain reaction analysis using Helicobacter specific urease A gene and 16S rRNA primers, bile pH measurement, and Helicobacter culture were performed. Helicobacter DNA was detected in 37.5%, and 31.3% by PCR with ureA gene, and 16S rRNA, respectively. The bile pH was not related to the presence of Helicobacter. The cultures were not successful. In conclusion, Helicobacter can be detected in the bile of patients with bile duct diseases. The possibility of pathogenesis of biliary tract diseases in humans by these organisms will be further investigated.
Adenocarcinoma/microbiology
;
Adult
;
Aged
;
Aged, 80 and over
;
Bile/microbiology*
;
Bile Duct Diseases/microbiology*
;
Bile Duct Neoplasms/microbiology
;
Cholelithiasis/microbiology
;
DNA Primers
;
DNA, Bacterial*
;
Helicobacter/isolation & purification*
;
Helicobacter/growth & development
;
Helicobacter/genetics
;
Human
;
Hydrogen-Ion Concentration
;
Middle Age
;
Pancreatic Neoplasms/microbiology
;
Polymerase Chain Reaction
6.Concomitant early avascular necrosis of the femoral head and acute bacterial arthritis by enteric Gram-negative bacilli in four oncologic patients.
Singapore medical journal 2013;54(5):e108-12
We present four cases of concomitant early (modified Ficat-Arlet stage I) avascular necrosis of the femoral head and acute bacterial arthritis of the hip joint by Gram-negative enteric bacilli. This was found in immunosuppressed oncologic patients whose clinical presentations and radiological findings were not entirely specific for joint sepsis. It is important to recognise the coexistence of these two pathologies, so as to avoid a delay in diagnosis and prevent significant morbidity and mortality.
Acute Disease
;
Adenocarcinoma
;
complications
;
microbiology
;
Adult
;
Arthritis, Infectious
;
complications
;
diagnosis
;
Bacillus
;
isolation & purification
;
Contrast Media
;
Female
;
Femur Head Necrosis
;
complications
;
diagnosis
;
Gram-Negative Bacteria
;
isolation & purification
;
Hodgkin Disease
;
complications
;
microbiology
;
Humans
;
Leukemia, Myeloid, Acute
;
complications
;
microbiology
;
Lung Neoplasms
;
complications
;
microbiology
;
Male
;
Middle Aged
;
Multiple Myeloma
;
complications
;
microbiology
;
Neoplasms
;
complications
;
microbiology
;
Risk Factors
;
Young Adult
7.Purulent Pericarditis Caused by Group G Streptococcus as an Initial Presentation of Colon Cancer.
Nam Ho KIM ; Jong Pil PARK ; Seong Hui JEON ; Youn Jeong LEE ; Hyeon Jong CHOI ; Keum Mo JEONG ; Jin Gu LEE ; Sun Pil CHOI ; Ji Hyun LIM ; Yang Ho KIM ; Yong Seok KIM ; Yong Moon KIM ; Min Ho HWANG ; Jin Woong CHO ; Young MOON ; Seok Kyu OH ; Jin Won JEONG
Journal of Korean Medical Science 2002;17(4):571-573
Bacterial pericarditis has been recognized as a rare disease since the development of antibiotics. Usually, the disease is associated with underlying conditions or a seeding of infection elsewhere to the pericardium. Here we describe a case of group G streptococcal pericarditis as an initial presentation of colon cancer. A 52-yr-old man was admitted because of dyspnea. An electrocardiogram showed a diffuse ST-segment elevation and a two-dimensional echocardiogram showed a large amount of pericardial effusion. A pericardiocentesis was done and purulent fluid was drained. Group G streptococci was cultured in pericardial fluid. The patient was treated with antibiotics and pericardiostomy with saline irrigation. A colonoscopy revealed a small mass with moderately differentiated adenocarcinoma in rectosigmoid colon. He underwent a mucosectomy and was recovered without any complication.
Adenocarcinoma/complications/*diagnosis/surgery
;
Colonic Neoplasms/complications/*diagnosis/surgery
;
Echocardiography
;
Electrocardiography
;
Humans
;
Male
;
Middle Aged
;
Pericardial Effusion
;
Pericarditis/complications/drug therapy/*microbiology/surgery
;
Streptococcal Infections/complications/drug therapy/*microbiology/surgery
;
Streptococcus/*classification
8.Effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma cells in vitro.
Xin SONG ; Hui-Xin CHEN ; Jie CHEN ; Ai-Ping BAI ; Xiao-Yan LI ; Wei CHEN ; Min-Hu CHEN
Chinese Journal of Oncology 2008;30(5):339-342
OBJECTIVETo investigate the effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma AGS cells.
METHODSWith experiment-control system of the wild-type CagA positive strain and isogenic CagA negative mutant strain of Helicobacter pyroli (Hp) were used as control and experimental groups, respectively. The cell contact, migration and invasion were examined by light and electron microscopy and invasion assay.
RESULTSThe AGS cells infected by Hp strain with positive wild-type CagA showed more severely changed tight junction, wider intercellular space, loss of cell contacts, and higher migrating and invasive ability.
CONCLUSIONHp CagA may lead to loss of cell contacting and higher migrating and invading ability of gastic cells, and accelerates the malignant progress of tumor.
Adenocarcinoma ; microbiology ; pathology ; ultrastructure ; Antigens, Bacterial ; genetics ; Bacterial Proteins ; genetics ; Cell Line, Tumor ; Cell Movement ; Extracellular Space ; Helicobacter pylori ; genetics ; pathogenicity ; Humans ; Intercellular Junctions ; ultrastructure ; Mutation ; Stomach Neoplasms ; microbiology ; pathology ; ultrastructure
9.Exploration of the association of H. pylori and EBV infection with cardiac and distal gastric adenocarcinoma among residents in Cixian County, a high-risk area of esophgeal cancer in Hebei province.
Dai-zhong WANG ; Xiang-hong ZHANG ; Wen-xin WU ; Yu-mei MA ; Ai-rong CUI ; Wei-na LIU ; Xue-min LI
Chinese Journal of Oncology 2012;34(6):446-449
OBJECTIVETo evaluate the H. pylori and Epstein-Barr virus infection in cardiac and distal gastric adenocarcinoma tissues in residents in Cixian county, a high risk area of esophageal cancer in Hebei province, and to explore the putative role of H. pylori and Epstein-Barr virus infection in the carcinogenesis of adenocarcinoma at different subsites of stomach.
METHODSH. pylori and Epstein-Barr virus latent membrane protein 1 (EBV-LMP1) immunopositivities were determined by Elivision(TM) plus immunohistochemical staining in 190 gastric adenocarcinoma tissues including 144 cases of cardiac adenocarcinoma and 46 cases of distal gastric adenocarcinoma. The relationship between H. pylori and Epstein-Barr virus infection and the subsite, Laurén type as well as other clinicopathological features of gastric adenocarcinoma were analyzed.
RESULTSNo significant difference was found between the H. pylori detection rates in cardiac and distal gastric adenocarcinomas(56.9% vs. 65.2%, P > 0.05). The detection rate of H. pylori in intestinal type was significantly higher than that in the diffuse type distal gastric adenocarcinomas (71.8% vs. 28.6%, P < 0.05). No positive expression of EBV-LMP1 was found in the gastric adenocarcinomas in this study.
CONCLUSIONSNo significant differences in H. pylori and EBV-LMP1 infections were found between cardiac and distal gastric adenocarcinomas in Cixian county. H. pylori infection is related with the intestinal type of distal gastric adenocarcinoma.
Adenocarcinoma ; microbiology ; pathology ; virology ; Aged ; Cardia ; China ; Epstein-Barr Virus Infections ; pathology ; Female ; Helicobacter Infections ; pathology ; Helicobacter pylori ; isolation & purification ; Humans ; Male ; Middle Aged ; Stomach Neoplasms ; microbiology ; pathology ; virology ; Viral Matrix Proteins ; metabolism
10.Life-threatening meningitis resulting from transrectal prostate biopsy.
Zhou-Jun SHEN ; Shan-Wen CHEN ; Hua WANG ; Xie-Lai ZHOU ; Ju-Ping ZHAO
Asian Journal of Andrology 2005;7(4):453-455
After antibiotic prophylaxis with metronidazole and levofloxacin, a transrectal sextant biopsy was performed under the guide of transrectal ultrasonography (TRUS) for a 75-year-old suspicious patient with prostate adenocarcinoma. Although antibiotics were also given after this procedure, the patient still developed fever, anxious, agrypnia and headache. Blood cultures remained negative. Lumbar puncture was performed and was consistent with Escherichia coli bacterial meningitis.
Adenocarcinoma
;
pathology
;
Aged
;
Anti-Infective Agents
;
administration & dosage
;
Anti-Infective Agents, Urinary
;
administration & dosage
;
Biopsy
;
adverse effects
;
Escherichia coli Infections
;
drug therapy
;
etiology
;
Humans
;
Levofloxacin
;
Male
;
Meningitis
;
etiology
;
microbiology
;
prevention & control
;
Metronidazole
;
administration & dosage
;
Ofloxacin
;
administration & dosage
;
Prostatic Neoplasms
;
pathology
;
Ultrasound, High-Intensity Focused, Transrectal