1.Expression of 24p3 and interleukin-17A in autoimmune hepatitis.
Bo HE ; Wen-da GAO ; Gui-qin SONG ; Chen-chen WANG ; Ming-li YANG ; Quan-sheng LIU
Chinese Journal of Hepatology 2007;15(9):709-710
Acute-Phase Proteins
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metabolism
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Animals
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Female
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Hepatitis, Autoimmune
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immunology
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metabolism
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pathology
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Interleukin-17
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metabolism
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Lipocalin-2
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Lipocalins
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metabolism
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Liver
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pathology
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Mice
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Mice, Inbred BALB C
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Oncogene Proteins
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metabolism
2.The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves.
Myung Hoo KIM ; Ji Young YANG ; Santi Devi UPADHAYA ; Hyun Jun LEE ; Cheol Heui YUN ; Jong K HA
Journal of Veterinary Science 2011;12(2):151-157
The purpose of our study was to investigate changes in immunological parameters induced by weaning stress (including milk restriction) in calves. Fifteen Holstein calves were subjected to weaning at 6 weeks of age. Blood samples were collected at -14, -7, -2, 1, 3, and 5 days post-weaning (DPW; 0 DPW = 42 days). Weaning caused significant (p < 0.01) increases in the neutrophil (NE):lymphocyte (LY) ratio at 5 DPW with a significant (p < 0.05) reduction of LYs. The concentration of acute-phase proteins (haptoglobin and serum amyloid A) also increased significantly (p < 0.05) at 3 and 5 DPW compared to -2 DPW. Levels of the iron-binding protein lactoferrin decreased significantly (p < 0.05) after weaning. Serum tumor necrosis factor-alpha and cortisol levels were elevated (p < 0.05) at 3 DPW, while those of serum interferon-gamma decreased (p < 0.05) at 1 and 3 DPW compared to levels observed before weaning. Weaning significantly (p < 0.05) decreased the percentage of CD25+ T cells in the peripheral blood. In conclusion, weaning stress affected the NE:LY ratio along with the levels of acute phase proteins, lactoferrin, cortisol, and inflammatory cytokines in the peripheral blood of calves. Weaning stress may induce an acute phase response possibly through the elevation of cortisol production and modulation of inflammatory cytokines.
Acute-Phase Proteins/*immunology/metabolism
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Acute-Phase Reaction/immunology/*veterinary
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Animals
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Cattle/*immunology
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Female
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Flow Cytometry
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Haptoglobins/analysis/immunology
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Hydrocortisone/blood/immunology
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Interferon-gamma/blood/immunology
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Lactoferrin/analysis/immunology
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Leukocyte Count/veterinary
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Leukocytes/cytology/*immunology
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Male
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Serum Amyloid A Protein/analysis/immunology
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Stress, Physiological/*physiology
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Tumor Necrosis Factor-alpha/blood/immunology
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Weaning
3.Rhamnogalacturonan II is a Toll-like receptor 4 agonist that inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells.
Sung Nam PARK ; Kyung Tae NOH ; Young Il JEONG ; In Duk JUNG ; Hyun Kyu KANG ; Gil Sun CHA ; Su Jung LEE ; Jong Keun SEO ; Dae Hwan KANG ; Tae Ho HWANG ; Eun Kyung LEE ; Byungsuk KWON ; Yeong Min PARK
Experimental & Molecular Medicine 2013;45(2):e8-
We evaluated the effectiveness of rhamnogalacturonan II (RG-II)-stimulated bone marrow-derived dendritic cells (BMDCs) vaccination on the induction of antitumor immunity in a mouse lymphoma model using EG7-lymphoma cells expressing ovalbumin (OVA). BMDCs treated with RG-II had an activated phenotype. RG-II induced interleukin (IL)-12, IL-1beta, tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) production during dendritic cell (DC) maturation. BMDCs stimulated with RG-II facilitate the proliferation of CD8+ T cells. Using BMDCs from the mice deficient in Toll-like receptors (TLRs), we revealed that RG-II activity is dependent on TLR4. RG-II showed a preventive effect of immunization with OVA-pulsed BMDCs against EG7 lymphoma. These results suggested that RG-II expedites the DC-based immune response through the TLR4 signaling pathway.
Acute-Phase Proteins/metabolism
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Adaptor Proteins, Vesicular Transport/metabolism
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Animals
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Antigens, CD14/metabolism
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Bone Marrow Cells/cytology/drug effects
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CD8-Positive T-Lymphocytes/*immunology
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Carrier Proteins/metabolism
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Cell Differentiation/drug effects
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Cell Nucleus/drug effects/metabolism
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Cell Proliferation/drug effects
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Cytokines/biosynthesis
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Dendritic Cells/cytology/drug effects/enzymology/*immunology
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Enzyme Activation/drug effects
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Lymphocyte Activation/*drug effects
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Membrane Glycoproteins/metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mitogen-Activated Protein Kinases/metabolism
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Myeloid Differentiation Factor 88/metabolism
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NF-kappa B/metabolism
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Neoplasms/immunology/*pathology
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Pectins/*pharmacology
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Phenotype
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Protein Transport/drug effects
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Receptors, Chemokine/metabolism
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Signal Transduction/drug effects
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T-Lymphocytes, Cytotoxic/cytology/drug effects
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Toll-Like Receptor 4/*agonists/metabolism