Introduction: Different solvents extraction was used to extract the good fatty acid composition of Dabai fruits. Nevertheless, solvents extraction may exhibit harmful effects. The present study was aimed to evaluate the safety of using supercritical carbon dioxide extraction (SCO2) of dabai pulp oil by acute toxicity study in Specific Pathogen Free (SPF) Sprague-Dawley (SD) rats. Methods: The CO pulp oil extract was prepared by SCO2 extraction of the freezedried pulp and was administered orally to SPF SD rats (consisted of 5 rats/sex/group) at upper limit dose 5000 mg/kg body weight (BW) for 14 days. The study includes the control and treatment groups, each consisting of 5 male and female rats. The rats were fed and allowed to drink sterilized water ad libitum. Fatty acid composition (FAC) of the extract was determined using GC-FID. Electrolytes and biochemical parameters in blood, as well as relative organs weight were measured. Results: The extract at a single dose of 5000 mg/kg did not cause any acute toxicity effects or mortality to the treatment of rats during observation periods in 14 days. FAC of the SCO2 extracted oil exhibited high content of palmitic and linoleic acids. The relative organs weights (ROW) and histopathology of rats were within normal range. Conclusion: Thus, the LD50 was estimated to be more than 5000 mg/kg of CO pulp oil extract and can be considered for further investigation for its therapeutic efficacy in a larger animal model
Acute toxicity

Introduction: Thymoquinone (TQ), a bioactive compound from Nigella sativa is known for its various medicinal properties. Due to the low solubility of TQ, nanostructured lipid carrier (NLC) has been used as a delivery system to improve its efficacy. Nevertheless, the effect of TQ-NLC when administered intravenously is unclear. This study investigated the acute toxicity profile of intravenous administration of TQ-NLC in an in vivo model. Methods: Twelve female Sprague dawley rats were assigned randomly into two groups (n=6); a control and a treatment group that received normal saline and 25 mg/kg TQ-NLC, respectively, via intravenous injection. The rats were observed for 14 days for any alterations to their usual physical conditions such as behaviour and mortality, body weight, food intake, organ-to-body weight ratio, and haematological, biochemical and histopathological profile. Results: There were no significant changes (p>0.05) in the body weight, food intake, organ-to-body weight ratio, and haematological, biochemical and histopathological profile between TQ-NLC treatment and the control group. However, inflammation was observed at the site of injection on the rat’s tail. Conclusion: Intravenous administration of TQ-NLC (25 mg/kg) did not exert acute toxic effect in female Sprague dawley rats. The data can be used as a basis to further develop TQNLC as a potential therapeutic drug.
Acute toxicity

The maximal doses of Pancreatine, in concentrations of 1.5%o and 3%o that can be given in mice, equivalent 0.450g/1ml distilled water/mouse (preparation 1.5%o and 0.90g/1ml distilled water/mouse (preparation 3%o, ie 200 times the dose for pigs, were administered in mice orally. After the trial of acute toxicity, our finding are as follows: After 72 hours signs of poisoning or deaths were not observed. LD50 could not be determined because of the potential low toxicity of the product
Acute Toxicity Tests ; Poisoning

A study on the fruit of Gardenia jasminoides has shown that concentration of chromatic compounds in the fruit of plant was 21,2% (w/w). The LD 50 of extraction from fruits determined as method of Behrens-Karber was relatively high (83g/kg). Therefore, the dose of 6-12 g can be used for safe treatment
Acute Toxicity Tests ; Fruit

There are some inapropriate problems: 1. The number of mice according to method from 30-100; 2. The weight of mice is 16-22g but most common is 19+/-1g(45 days);3. If oral dosage: 0.2 ml/10 g mice, if body weight:19+/- 1 g: the same dose of 0.4-0.5 ml/mice; 4. As calculating method of Behrens, the number of mice can provide LD 50 with small adds; 5. Safe maximal dose; 6. The mice is monitored mainly during first 24 hours. The monitoring time may be 36-48 hours; 7. The morphological, frequency and amplitude change of all waves in electrocardiogram and encephalogram should be nomitored.
Acute Toxicity Tests

There different extract solutions of Kim Ngan Hoa were used in this study. The experimental results showed that: Kim Ngan Hoa has analgesic effect on two experimental models, hotplate and writing test on mice. Beside of analgesic effect, it has significantly acute antiinflammatory effect on rats. The effects of flavonoid and the alcoholic extract are potent than the effects of the water extract. Three extract solutions from Kim Ngan Hoa have also inhibitory effect in vitro the developing of three bacteria: Staphylococcus aureus, E. coli and Pseudomonas aeruginosa. Three preparations from Kim Ngan Hoa have very low acute toxicity
Acute Toxicity Tests ; Research ; Pharmaceutical Preparations ; Toxicity

OBJECTIVETo analyze the results of the first-stage toxicological evaluation of 1 502 pesticide samples.

METHODSThe classification of the 1 502 pesticide samples was analyzed, and the experimental results of the samples in different years were compared.

RESULTSMost of the 1 502 pesticide samples were insecticides, accounting for 52.5% of all, followed by bactericides and herbicides. In the 5 years, the proportion of biogenic insecticides showed a significant rising trend (χ² = 11.426, P < 0.05). The proportion of single pesticides was 65.8%; mixed pesticides accounted for 32.7%; original pesticides accounted for only 1.5%. From 2008 to 2012, most pesticides had low toxicity, regardless of the exposure route (via the mouth, skin, or respiratory tract). Acute oral and dermal toxicity tests showed that pesticides with moderate toxicity declined year by year (oral exposure χ² = 18.036, P < 0.01; dermal exposure χ² = 40.482, P < 0.01). There was a small proportion of pesticides with high toxicity. We did not detect any pesticide with extreme toxicity. Acute skin irritation and eye irritation test showed an upward trend in proportion of non-irritating pesticides (χ² = 77.110, P < 0.01; χ² = 12.693, P < 0.05), while the proportion of medium-irritation pesticides decreased significantly (χ² = 18.941, P < 0.01; χ² = 13.129, P < 0.05). Sensitization test showed that all samples were weak sensitizers.

CONCLUSIONThe major type of investigated pesticides was insecticide. Most samples were single pesticides, and there was a certain proportion of mixed pesticides. Novel pesticides such as bio-pesticides are the development tendency. The tested pesticides were mainly low-toxicity pesticides, with a certain proportion of medium- and high-toxicity pesticides. Personal protection should be strengthened during production and use of pesticides.

The traditional remedy of “Thap tu vi chi khai suyen” was derived from two traditional remedies of “Tam ao thang” and “Nhi tran thang”. It was produced by the Institute of Military Traditional Medicine and was presented as instant tea which met to all basic standards of Health Ministry. Studying acute toxicity (LD50) was performed on 50 white rats with two sexes; studying semi-polychronic toxicity was performed on 14 rabbits with two sexes. Results: sudying acute toxicity, LD50 was not found because the dose causing mice dead was not found. Studying semi-polychronic toxicity, there were no significant differences about biochemical and hematogenic indices between study group and control group.
Medicine, Traditional ; Acute Toxicity Tests

Background: Chlorophytum laxum R. Br. (Anthericaceae) are medicinal plants used by people to restore and improve the health of women after birth to boost energy quickly. Objectives: To anounce the results of acute toxicity and cytotoxic effect of Chlorophytum laxum R. Br. (Anthericaceae). Subjects and method: Using an acute toxicity test on Chlorophytum laxum R. Br. (Anthericaceae) collected at Thua Thien Hue province on September, 2005. The data was analysed by bio statistic based on the data analysis of Microsoft Excel. Results:At the dose of 120g/kg body weigh we have not found the oral LD\xac 50 so of the aqueous extract of Chlorophytum laxum R. Br. in mice. Methyl caffeate and 1,2Di-O-(9Z, 12Z- octadecatrienoyl) -3-O-beta-D-galactopyranosylglycerol were isolated from the methanol extract of the roots from Chlorophytum laxum R. Br. have antitumor activity in lung, liver cancer cells and pericardioma. Conclusion: This is the first scientifically published article on the biological effects of Chlorophytum laxum R. Br plant roots, particularly the cytotoxic effects on common cancer cell such as liver cancer, lung cancer from the isolated compounds in pure.
Acute toxicity ; cytotoxic effect of Chlorophytum laxum

Cot khi cu was studied on experiment animals. Pain relief effect was evaluated with 1% extract, sedative and hypnotic effect was compared with hexobarbital and acute toxicity was assessed by Lichfield Wilcozon Method. Results showed that raw and processed Cot khi cu did not cause acute toxicity, with a dose of 200 folds of clinical dose. In raw and processed Cot khi cu, there is no defference in pain relief effect. Roast Cot khi cu gave a better sedative effect than the raw Cot khi cu
Acute Toxicity Tests ; Animals ; Biochemistry ; drugs