The maximal doses of Pancreatine, in concentrations of 1.5%o and 3%o that can be given in mice, equivalent 0.450g/1ml distilled water/mouse (preparation 1.5%o and 0.90g/1ml distilled water/mouse (preparation 3%o, ie 200 times the dose for pigs, were administered in mice orally. After the trial of acute toxicity, our finding are as follows: After 72 hours signs of poisoning or deaths were not observed. LD50 could not be determined because of the potential low toxicity of the product
Acute Toxicity Tests ; Poisoning

A study on the fruit of Gardenia jasminoides has shown that concentration of chromatic compounds in the fruit of plant was 21,2% (w/w). The LD 50 of extraction from fruits determined as method of Behrens-Karber was relatively high (83g/kg). Therefore, the dose of 6-12 g can be used for safe treatment
Acute Toxicity Tests ; Fruit

There are some inapropriate problems: 1. The number of mice according to method from 30-100; 2. The weight of mice is 16-22g but most common is 19+/-1g(45 days);3. If oral dosage: 0.2 ml/10 g mice, if body weight:19+/- 1 g: the same dose of 0.4-0.5 ml/mice; 4. As calculating method of Behrens, the number of mice can provide LD 50 with small adds; 5. Safe maximal dose; 6. The mice is monitored mainly during first 24 hours. The monitoring time may be 36-48 hours; 7. The morphological, frequency and amplitude change of all waves in electrocardiogram and encephalogram should be nomitored.
Acute Toxicity Tests

There different extract solutions of Kim Ngan Hoa were used in this study. The experimental results showed that: Kim Ngan Hoa has analgesic effect on two experimental models, hotplate and writing test on mice. Beside of analgesic effect, it has significantly acute antiinflammatory effect on rats. The effects of flavonoid and the alcoholic extract are potent than the effects of the water extract. Three extract solutions from Kim Ngan Hoa have also inhibitory effect in vitro the developing of three bacteria: Staphylococcus aureus, E. coli and Pseudomonas aeruginosa. Three preparations from Kim Ngan Hoa have very low acute toxicity
Acute Toxicity Tests ; Research ; Pharmaceutical Preparations ; Toxicity

OBJECTIVETo analyze the results of the first-stage toxicological evaluation of 1 502 pesticide samples.

METHODSThe classification of the 1 502 pesticide samples was analyzed, and the experimental results of the samples in different years were compared.

RESULTSMost of the 1 502 pesticide samples were insecticides, accounting for 52.5% of all, followed by bactericides and herbicides. In the 5 years, the proportion of biogenic insecticides showed a significant rising trend (χ² = 11.426, P < 0.05). The proportion of single pesticides was 65.8%; mixed pesticides accounted for 32.7%; original pesticides accounted for only 1.5%. From 2008 to 2012, most pesticides had low toxicity, regardless of the exposure route (via the mouth, skin, or respiratory tract). Acute oral and dermal toxicity tests showed that pesticides with moderate toxicity declined year by year (oral exposure χ² = 18.036, P < 0.01; dermal exposure χ² = 40.482, P < 0.01). There was a small proportion of pesticides with high toxicity. We did not detect any pesticide with extreme toxicity. Acute skin irritation and eye irritation test showed an upward trend in proportion of non-irritating pesticides (χ² = 77.110, P < 0.01; χ² = 12.693, P < 0.05), while the proportion of medium-irritation pesticides decreased significantly (χ² = 18.941, P < 0.01; χ² = 13.129, P < 0.05). Sensitization test showed that all samples were weak sensitizers.

CONCLUSIONThe major type of investigated pesticides was insecticide. Most samples were single pesticides, and there was a certain proportion of mixed pesticides. Novel pesticides such as bio-pesticides are the development tendency. The tested pesticides were mainly low-toxicity pesticides, with a certain proportion of medium- and high-toxicity pesticides. Personal protection should be strengthened during production and use of pesticides.

The traditional remedy of “Thap tu vi chi khai suyen” was derived from two traditional remedies of “Tam ao thang” and “Nhi tran thang”. It was produced by the Institute of Military Traditional Medicine and was presented as instant tea which met to all basic standards of Health Ministry. Studying acute toxicity (LD50) was performed on 50 white rats with two sexes; studying semi-polychronic toxicity was performed on 14 rabbits with two sexes. Results: sudying acute toxicity, LD50 was not found because the dose causing mice dead was not found. Studying semi-polychronic toxicity, there were no significant differences about biochemical and hematogenic indices between study group and control group.
Medicine, Traditional ; Acute Toxicity Tests

The study was carried out with healthy white mice of the strain of Swiss, weighed 202 g, fed properly in good supply of water and food. Results a pain relief and narcotic effects of the material. An intensive dose 200 times higher than usual clinical dose did not manifest toxicity in the animal
Medicine, Traditional ; Chemistry, Pharmaceutical ; Acute Toxicity Tests

Cot khi cu was studied on experiment animals. Pain relief effect was evaluated with 1% extract, sedative and hypnotic effect was compared with hexobarbital and acute toxicity was assessed by Lichfield Wilcozon Method. Results showed that raw and processed Cot khi cu did not cause acute toxicity, with a dose of 200 folds of clinical dose. In raw and processed Cot khi cu, there is no defference in pain relief effect. Roast Cot khi cu gave a better sedative effect than the raw Cot khi cu
Acute Toxicity Tests ; Animals ; Biochemistry ; drugs

Administration, Oral ; Animals ; Mice ; Nanoparticles ; toxicity ; Particle Size ; Titanium ; toxicity ; Toxicity Tests, Acute

OBJECTIVES: Titanium dioxide (TiO2), a common nanoparticle widely used in industrial production, is one of nano-sized materials. The purpose of this study was to determine the acute and chronic toxicity of TiO2 using different size and various concentrations on Daphnia magna. METHODS: In the acute toxicity test, four concentrations (0, 0.5, 4, and 8 mM) for TiO2 with 250 or 500 nm and five concentrations (0, 0.25, 0.5, 0.75, and 1 mM) for TiO2 with 21 nm were selected to analyze the toxic effect to three groups of ten daphnia neonates over 96 hours. In addition, to better understand their toxicity, chronic toxicity was examined over 21 days using 0, 1, and 10 mM for each type of TiO2. RESULTS: Our results showed that all organisms died before the reproduction time at a concentration of 10 mM of TiO2. In addition, the exposure of anatase (21 nm) particles were more toxic to D. magna, comparing with that of anatase (250 nm) and rutile (500 nm) particles. CONCLUSIONS: This study indicated that TiO2 had adverse impacts on the survival, growth and reproduction of D. magna after the 21days exposure. In addition, the number of test organisms that were able to reproduce neonates gradually were reduced as the size of TiO2 tested was decreased.
Daphnia ; Humans ; Infant, Newborn ; Nanoparticles ; Reproduction ; Titanium ; Toxicity Tests, Acute