1.Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.
Alfredo GARCIA ; Remigio MARTINEZ ; Jose Manuel BENITEZ-MEDINA ; David RISCO ; Waldo Luis GARCIA ; Joaquin REY ; Juan Manuel ALONSO ; Javier Hermoso DE MENDOZA
Journal of Veterinary Science 2013;14(4):491-494
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
Actinomycetales/*isolation & purification
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Actinomycetales Infections/diagnosis/microbiology/*veterinary
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Animals
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Cattle
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Cattle Diseases/*diagnosis/microbiology
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Fluorescent Dyes/*diagnostic use
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Horse Diseases/*diagnosis/microbiology
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Horses
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Limit of Detection
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Real-Time Polymerase Chain Reaction/*methods/veterinary
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Reproducibility of Results
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Sheep
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Sheep Diseases/*diagnosis/microbiology
2.A Case of Catheter-Related Bacteremia of Tsukamurella pulmonis.
Hyoeun Eun SHIM ; Heungsup SUNG ; Seung Mi BAEK ; Seung NAMGUNG ; Mi Na KIM ; Yong Gyun KIM ; Gyu Hyung LEE
The Korean Journal of Laboratory Medicine 2009;29(1):41-47
Tsukamurella pulmonis is an aerobic actinomycete. We report a catheter-related bacteremia of T. pulmonis. A 39 yr-old male with ALL was hospitalized to receive bone marrow transplantation (BMT). Although the patient developed a high fever at the 7th hospital day (HD), it subsided with vancomycin treatment, and he received BMT at 9th HD. Fever resurged at 16th HD despite sustained treatment with vancomycin, meropenem, and amphotericin B, but subsided with removal of Hickman catheter (HC) at 19th HD. Three sets of blood cultures comprising one from the HC and two from venipunctures were taken at 7th, 16th, and 19th HD, and the distal tip of the HC was also cultured. The aerobic vials of all 3 HC-withdrawn blood cultures and one peripheral blood culture taken at 19HD and the HC tip culture grew long, straight, thin gram-positive rods that were positive on modified Kinyoun stain. This organism showed tiny, rough, grey colonies after 3-day incubation and grew to large flat colonies when incubation was extended. It was catalase-positive, urease-positive, and alkaline-slant/alkaline-deep on triple sugar iron agar, and hydrolyzed hypoxanthine. The sequence of 1,296 base pairs of 16S rRNA of this organism showed a 100.0% homology with the published sequence of T. pulmonis DSM 44142T. To our knowledge, this is the first report of T. pulmonis bacteremia in Korea.
Actinomycetales/classification/genetics/isolation & purification
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Actinomycetales Infections/diagnosis/*microbiology/therapy
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Adult
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Bacteremia/*diagnosis/microbiology/therapy
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Bone Marrow Transplantation
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Catheter-Related Infections/*microbiology
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Humans
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Leukemia, Myeloid, Acute/therapy
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Male
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Phylogeny
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RNA, Ribosomal, 16S/genetics
3.Imaging and pathological findings of AIDS complicated by pulmonary Rhodococcus equi infection.
Hong-Jun LI ; Jing-Liang CHENG
Chinese Medical Journal 2011;124(7):968-972
BACKGROUNDRhodococcus equi (R. equi) infection commonly occurs in grazing areas, especially in patients with AIDS or with T-lymphocyte immuno-deficiencies. Literature reviews revealed that cases radiologically and pathologically diagnosed of AIDS complicated by R. equi infection are rare. This study aimed to investigate the imaging features and pathological basis of AIDS complicated by pulmonary R. equi infection.
METHODSA total of 13 cases of AIDS complicated by pulmonary R. equi infection were retrospectively analyzed based on their imaging, bacterial culture and pathological data, including 10 cases by chest CT scanning and X-ray radiology and 3 cases by only X-ray radiology. All 13 cases were definitely diagnosed by bacterial culture, including one by CT-guided pulmonary puncture with following H&E staining and periodic acid-Schiff (PAS) staining for diagnostic biopsy and another one by bronchial biopsy with following H&E staining and PAS staining for pathological diagnosis. The imaging findings and the pathological findings of AIDS complicated by pulmonary R. equi infection were compared and evaluated.
RESULTSTotally 9 subjects (70%) had radiological demonstrations of central ball liked high density shadows in unilateral pulmonary hilus areas; 10 (77%), cavities and liquefied levels; 3 (23%), pleural effussion. The foci were found in pulmonary inner zone in 10 subjects (77%) and in pulmonary outer zone in one subject (7%). The pathological findings included intra-alveolar hemorrhage, lymphocyte infiltration and granulation tissue proliferation, which were in line with the pathological process of necrotic pneumonia. After 8-month follow-up of anti-R. equi therapy of these 13 cases, 9 cases had obviously decreased or shrunk pulmonary cavities, one died, one missed follow-up, one completely absorbed foci and one did not receive reexaminations.
CONCLUSIONSThe radiological demonstrations of AIDS complicated by pulmonary R. equi infection are central ball liked high density areas in unilateral pulmonary hilus area, parenchymal changes, secondary cavities, ground glass liked changes in the lung fields, nodules and treeinbuds sign, which are characteristic rather than specific.
Acquired Immunodeficiency Syndrome ; diagnosis ; diagnostic imaging ; Actinomycetales Infections ; diagnosis ; diagnostic imaging ; Adult ; Female ; Humans ; Lung Diseases ; microbiology ; Male ; Middle Aged ; Radiography ; Rhodococcus equi ; pathogenicity ; Young Adult
4.A Case of Necrotizing Fasciitis Due to Streptococcus agalactiae, Arcanobacterium haemolyticum, and Finegoldia magna in a Dog-bitten Patient with Diabetes.
Sungsil LEE ; Kyoung Ho ROH ; Chang Ki KIM ; Dongeun YONG ; Jun Yong CHOI ; Jin Woo LEE ; Kyungwon LEE ; Yunsop CHONG
The Korean Journal of Laboratory Medicine 2008;28(3):191-195
We report a case of necrotizing fasciitis involving Streptococcus agalactiae, Arcanobacterium haemolyticum, and Finegoldia magna in a 36-yr-old female diabetic patient, which started after a minor dog bite to the toe of the patient. This case suggested that a trivial infection after a minor dog bite in an immunocompromised patient such as diabetes patient could result in a significant complication such as necrotizing fasciitis. The life-threatening infection was cured by timely above-the-knee amputation, as well as penicillin G and clindamycin therapy.
Actinomycetales Infections/diagnosis/microbiology
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Adult
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Animals
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Anti-Bacterial Agents/therapeutic use
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Arcanobacterium
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Bites and Stings/*complications
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Clindamycin/therapeutic use
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Diabetes Mellitus/diagnosis/therapy
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*Dogs
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Fasciitis, Necrotizing/diagnosis/drug therapy/*microbiology
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Female
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Gram-Positive Bacterial Infections/diagnosis/microbiology
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Humans
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Penicillin G/therapeutic use
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Peptostreptococcus
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Streptococcal Infections/diagnosis/microbiology
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Streptococcus agalactiae