No abstract available.
Actinobacillus* ; Aggregatibacter actinomycetemcomitans* ; Clone Cells* ; Cloning, Organism*

No abstract available.
Actinobacillus* ; Aggregatibacter actinomycetemcomitans* ; Animals ; Osteoclasts ; Rats*

No abstract available.
Actinobacillus* ; Aggregatibacter actinomycetemcomitans* ; Apoptosis* ; Humans ; Jurkat Cells*

No abstract available.
Actinobacillus* ; Aggregatibacter actinomycetemcomitans* ; Inflammation* ; Interleukin-6* ; Periodontal Pocket* ; Prevotella intermedia* ; Prevotella*

This study was performed to investigate the effects of Immunomodulating factor (IMF), derived from Actinobacillus actinomycetemcomitans, on various immune cells in the mouse spleen. A single dose of IMF (10 microgram/kg) was administer-ed to BALB/c mice by intraperitoneal injection. After the mice were sacrificed in groups of five at 6 h and 24 h, the spleens were removed. The immunocytochemical characterization of the immune cells was carried out using the various monoclonal antibodies in cryostat-cut sections. We demonstrated in this study a strong stimulating effect of IMF on dendritic cells and B lymphocytes in the mouse spleen after IMF administration. The MOMA-1(+) immunoreactivity on the marginal metallophilic macrophages in the splenic marginal zone disappeared 6 h and reappeared 24 h after IMF treatment. However, various subpopulations of T lymphocytes, CD3(+), CD4(+), CD8(+), TCRalpha, beta(+) and Vbeta8(+) T cells in the mouse spleen did not show any significant change in their distributional pattern after IMF treatment. Dendritic cells were found to be increased in number in the periarterial lymphatitc sheath, and B lymphocytes were also increased in number in the lymphoid follicles of the spleen after IMF injection. In conclusion, IMF exhibited a potent stimulative effect on dendritic cells and B lymphocytes in vivo.
Actinobacillus* ; Aggregatibacter actinomycetemcomitans* ; Animals ; Antibodies, Monoclonal ; B-Lymphocytes ; Dendritic Cells* ; Injections, Intraperitoneal ; Lymphocytes* ; Macrophages* ; Mice* ; Spleen* ; T-Lymphocytes

The purpose of this study was to investigate and compare the frequence of 4 periodontal pathogens in the supra- and subgingival plaque in periodontally healthy subjects. Twenty adult individuals aged 22 to 28 years (mean age 23.65 years) participated in this study. All subjects had no pocket sites more than 3 mm deep, and the sites selected for sampling were all negative for bleeding. After drying and isolation of the sites with cotton rolls, supragingival plaque was sampled using sterile periodontal curette. Each plaque sample was placed in individual tubes containing 500 ml of 1X PBS. After removal of the supragingival sample and any remaining supragingival plaque, subgingival plaque samples were taken from the same sites using sterile curette and placed in similar individual tubes. Identification of 4 putative periodontal pathogens from the samples was performed by polymerase chain reaction based on 16S rDNA. Chi-square test was employed to identify significant explanatory variables for the presence of the 4 periodontal pathogens. The data show that Actinobacillus actinmycetemcomitans, Porphyromonas gingivalis, Bacteroides forsythus, and Fusobacterium nucleatum occurred in 16.9%, 14.4%, 52.5%, and 80.6%, respectively. No significant differences were noted in the periodontal pathogens between supra- and subgingival plaques according to the kind of teeth. However, the incisors were at higher risk for harboring F. nucleatum (p <0.05). Conclusion: These results reveal that anaerobic periodontal pathogens can be detected in supragingival plaques. Supragingival plaque may function as a reservoir of periodotopathogens.
Actinobacillus ; Adult ; Aggregatibacter actinomycetemcomitans ; Bacteroides ; DNA, Ribosomal ; Fusobacterium nucleatum ; Hemorrhage ; Humans ; Incisor ; Periodontitis* ; Polymerase Chain Reaction ; Porphyromonas gingivalis ; Prevalence* ; Tooth ; Young Adult*

OBJECTIVEThe aim of this study was to investigate effects of immunodeficiency on the periodontal infection characters of the specific pathogens of juvenile periodontitis.

METHODSA total of 36 immunodeficient guinea pigs produced by twice whole-body irradiation with 60Co were divided randomly into four groups, in which Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and A. actinomycetemcomitans with P. gingivalis were inoculated into the gingival sulcus of two mandibular incisors respectively. The pigs in the control group did not receive any inoculation. At 2, 3 and 6 weeks after inoculation, three animals in each group were sacrificed successively. Clinical and histological examinations were used to examine the changes in the periodontal tissues. The other 36 normal guinea pigs were divided into four groups and treated in a similar way described above.

RESULTSSignificant periodontal damages were noted in immunodeficient pigs inoculated with A. actinomycetemcomitans, P. gingivalis or A. actinomycetemcomitans and P. gingivalis in 2 and 3 weeks after bacterial inoculation. The damages were more severe than in the normal groups. The immunodeficient groups demonstrated larger numbers of osteoclasts than the normal groups (P < 0.05).

CONCLUSIONThe loss of periodontal tissue in immunodeficient hosts is much serious than those with normal defence system, after they are infected with A. actinomycetemcomitans and P. gingivalis. Abnormal defence system in hosts may play an important role in onset and development of juvenile periodontitis.

Actinobacillus Infections ; immunology ; Aggregatibacter actinomycetemcomitans ; Aggressive Periodontitis ; immunology ; microbiology ; Animals ; Bacteroidaceae Infections ; immunology ; Female ; Immunocompromised Host ; immunology ; Male ; Porphyromonas gingivalis ; Random Allocation

A 73-year-old man was admitted for intermittent episodes of fever and chills for 3 months. He had been implanted with a permanent pacemaker to control tachy-bradycardia syndrome 7 months before admission. Blood cultures were positive for Actinobacillus actinomycetemcomitans and a 99mTc-hexamethylpropylene amine oxime (99mTc-HMPAO) WBC scan revealed inflammation on the pacemaker lead in extracardiac site. Oral examination revealed several dental caries. The patient was treated with intravenous ceftriaxone, followed by oral ciprofloxacin without removal of the infected pacemaker lead. He was doing well 10 months without febrile episodes after discontinuation of antibiotics. This report describes the first case of A. actinomycetemcomitans bacteremia associated with a pacemaker lead and localized by 99mTc-HMPAO WBC scan
Actinobacillus* ; Aged ; Aggregatibacter actinomycetemcomitans* ; Anti-Bacterial Agents ; Bacteremia* ; Ceftriaxone ; Chills ; Ciprofloxacin ; Dental Caries ; Diagnosis, Oral ; Fever ; Humans ; Inflammation ; Technetium Tc 99m Exametazime

A 73-year-old man was admitted for intermittent episodes of fever and chills for 3 months. He had been implanted with a permanent pacemaker to control tachy-bradycardia syndrome 7 months before admission. Blood cultures were positive for Actinobacillus actinomycetemcomitans and a 99mTc-hexamethylpropylene amine oxime (99mTc-HMPAO) WBC scan revealed inflammation on the pacemaker lead in extracardiac site. Oral examination revealed several dental caries. The patient was treated with intravenous ceftriaxone, followed by oral ciprofloxacin without removal of the infected pacemaker lead. He was doing well 10 months without febrile episodes after discontinuation of antibiotics. This report describes the first case of A. actinomycetemcomitans bacteremia associated with a pacemaker lead and localized by 99mTc-HMPAO WBC scan
Actinobacillus* ; Aged ; Aggregatibacter actinomycetemcomitans* ; Anti-Bacterial Agents ; Bacteremia* ; Ceftriaxone ; Chills ; Ciprofloxacin ; Dental Caries ; Diagnosis, Oral ; Fever ; Humans ; Inflammation ; Technetium Tc 99m Exametazime

200 subgingival specimens collected from 100 healthy periodontal adults of Thanhliet, Thanhtri, Hanoi, from 100 periodontitis adults of Odonto-stomatology Departments of Dongda and Thanhnhan Hospital were cultured and isolated for Actinobacillus actinomycetemcomitans and Capnocytophaga gingivitis. The prevalence of Aa and Cg in the periodontitis group is clearly different from that in the healthy periodontal one. The higher rate of Cg compared with the rate of Aa is found in female with periodontitis. Both Aa and Cg are sensitive to ampicillin, tetracycline, and chloramphenicole. Their sensitivity to erythromycin and sulfamethoxazole/ trimethoprime changes. Cg is resistant to amikacin. TSBV medium used for culturing and isolating Aa has improved to be suitable to Laboratories in Vietnam.
Actinobacillus actinomycetemcomitans ; Gingivitis