1.Staurosporine and cytochalasin D induce chondrogenesis by regulation of actin dynamics in different way.
Minjung KIM ; Kyung SONG ; Eun Jung JIN ; Jongkyung SONN
Experimental & Molecular Medicine 2012;44(9):521-528
Actin cytoskeleton has been known to control and/or be associated with chondrogenesis. Staurosporine and cytochalasin D modulate actin cytoskeleton and affect chondrogenesis. However, the underlying mechanisms for actin dynamics regulation by these agents are not known well. In the present study, we investigate the effect of staurosporine and cytochalasin D on the actin dynamics as well as possible regulatory mechanisms of actin cytoskeleton modulation. Staurosporine and cytochalasin D have different effects on actin stress fibers in that staurosporine dissolved actin stress fibers while cytochalasin D disrupted them in both stress forming cells and stress fiber-formed cells. Increase in the G-/F-actin ratio either by dissolution or disruption of actin stress fiber is critical for the chondrogenic differentiation. Cytochalasin D reduced the phosphorylation of cofilin, whereas staurosporine showed little effect on cofilin phosphorylation. Either staurosporine or cytochalasin D had little effect on the phosphorylation of myosin light chain. These results suggest that staurosporine and cytochalasin D employ different mechanisms for the regulation of actin dynamics and provide evidence that removal of actin stress fibers is crucial for the chondrogenic differentiation.
Actin Cytoskeleton/*drug effects
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Actins/metabolism
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Animals
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Cell Differentiation/*drug effects
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Cells, Cultured
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Chickens
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Chondrogenesis/*drug effects
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Cytochalasin D/*pharmacology
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Mesoderm/cytology/drug effects
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Myosin Light Chains/metabolism
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Nucleic Acid Synthesis Inhibitors/*pharmacology
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Phosphorylation
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Staurosporine/*pharmacology
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Stress Fibers/drug effects
2.Protective effect of catalpolon destruction of tight junctions of high glucose induced BMECs.
Li ZOU ; Ke LIU ; Hui-Feng ZHU ; Shan FENG
China Journal of Chinese Materia Medica 2018;43(20):4118-4124
This paper aimed to observe the protective effect of catalpol on the high glucose induced destruction of tight junctions of rat primary brain microvascular endothelial cells (BMECs). Catalpol co-administrated with high glucose increased BMECs survival, decreased its ET-1 secretion, and improved transmembrane electrical resistance in a time-dependent manner. Furthermore, transmission electron microscopy was used to observe catalpol's protective effect on tight junction. Fluorescence staining displayed that catalpol reversed the rearrangement of the cytoskeleton protein F-actin and up-regulated the tight junction proteins claudin-5 and ZO-1, which were further demonstrated by the mRNA expression levels of claudin-5, occludin, ZO-1, ZO-2, ZO-3, -actintin, vinculin and cateinins. This study indicated that catalpol reverses the disaggregation of cytoskeleton actin in BMECs and up-regulates the expression of tight junction proteins, such as claudin-5, occludin, and ZO-1, and finally alleviates the increase in high glucose-induced BMECs injury.
Actin Cytoskeleton
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drug effects
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Actins
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metabolism
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Animals
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Brain
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cytology
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Cells, Cultured
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Claudin-5
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metabolism
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Endothelial Cells
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drug effects
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Glucose
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Iridoid Glucosides
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pharmacology
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Phosphoproteins
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Rats
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Tight Junctions
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drug effects
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Zonula Occludens-1 Protein
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metabolism
3.Role of actin microfilament in hyposmotic membrane stretch-induced increase in muscarinic current of guinea-pig gastric myocytes.
Zuo-Yu WANG ; Yong-Chun YU ; Yi-Feng CUI ; Lin LI ; Hui-Shu GUO ; Zai-Liu LI ; Wen-Xie XU
Acta Physiologica Sinica 2003;55(2):177-182
To investigate the relationship between cytoskeleton and hyposmotic membrane stretch-induced increase in muscarinic current, the role of actin microfilament in hyposmotic membrane stretch-induced increase in muscarinic current was studied with the whole-cell patch clamp technique in guinea-pig gastric myocytes. In this study, the muscarinic current was induced by carbachol (50 micromol/L) or GTPgammaS (0.5 mmol/L). The results showed that hyposmotic superfusate (202 mOsmol/L) increased carbachol-induced current (I(CCh)) by 145+/-27% and increased GTPgammaS-induced current by 183+/-30%; but in the presence of cytochalasin-B (Cyt-B, 20 micromol/L), an actin cytoskeleton disruptor, hyposmotic membrane stretch increased I(CCh) by 70+/-6%. However, hyposmotic membrane stretch induced increase in I(CCh) was potentiated to 545+/-81% by phalloidin (20 micromol/L), an actin microfilament stabilizer. The results demonstrated that hyposmotic membrane stretch increased the muscarinic currents induced by carbachol or GTPgammaS and that the actin microfilament is involved in the process in guinea-pig gastric myocytes.
Actin Cytoskeleton
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physiology
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Animals
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Carbachol
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pharmacology
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Female
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Guinea Pigs
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Male
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Membrane Potentials
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drug effects
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Myocytes, Smooth Muscle
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physiology
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Osmotic Pressure
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Patch-Clamp Techniques
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Pyloric Antrum
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cytology
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Receptors, Muscarinic
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physiology
4.Ginseng Total Saponin Improves Podocyte Hyperpermeability Induced by High Glucose and Advanced Glycosylation Endproducts.
Tae Sun HA ; Ji Young CHOI ; Hye Young PARK ; Jin Seok LEE
Journal of Korean Medical Science 2011;26(10):1316-1321
Early diabetic nephropathy is characterized by glomerular hyperpermeability as a result of impaired glomerular filtration structure caused by hyperglycemia, glycated proteins or irreversible advanced glycosylation endproducts (AGE). To investigate the effect of ginseng total saponin (GTS) on the pathologic changes of podocyte ZO (zonula occludens)-1 protein and podocyte permeability induced by diabetic conditions, we cultured mouse podocytes under: 1) normal glucose (5 mM, = control); 2) high glucose (HG, 30 mM); 3) AGE-added; or 4) HG plus AGE-added conditions and treated with GTS. HG and AGE increased the dextran filtration of monolayered podocytes at early stage (2-8 hr) in permeability assay. In confocal imaging, ZO-1 colocalized with actin filaments and beta-catenin at cell contact areas, forming intercellular filtration gaps. However, these diabetic conditions suppressed ZO-1 immunostainings and disrupted the linearity of ZO-1. In Western blotting, diabetic conditions also decreased cellular ZO-1 protein levels at 6 hr and 24 hr. GTS improved such quantitative and qualitative changes. These findings imply that HG and AGE have an influence on the redistribution and amount of ZO-1 protein of podocytes thereby causing hyperpermeability at early stage, which can be reversed by GTS.
Actin Cytoskeleton/metabolism
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Animals
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Cell Line
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Diabetic Nephropathies/physiopathology
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Glomerular Filtration Rate
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Glucose/*pharmacology
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Glycosylation End Products, Advanced/*pharmacology
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Hyperglycemia/physiopathology
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Membrane Proteins/*metabolism
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Mice
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*Panax
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Permeability/drug effects
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Phosphoproteins/*metabolism
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Plant Preparations/*pharmacology
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Podocytes/drug effects/pathology/physiology
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Saponins/*pharmacology
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beta Catenin/metabolism