2.In vitro study of oral Candida albicans in virulence from HIV-positive individuals.
Xiao-song LIU ; Hong-wei LIU ; Zi-jie GUO ; Wen-min LUAN
Chinese Journal of Stomatology 2005;40(3):211-214
OBJECTIVETo study the influence of Candida albicans on human immunodeficiency virus (HIV)-positive individuals susceptible to oral candidiasis.
METHODSIn vitro secreted aspartyl proteinase activities, adhesion to healthy buccal epithelial cells of Candida albicans isolates from oral cavities of subjects with and without HIV infection were measured.
RESULTSThe pathogenetic isolates of Candida albicans from HIV-positive patients were significantly lower than that from HIV-negative subjects (P < 0.01) in secreted aspartyl proteinase activities and adhesion to buccal epithelial cells. There was no difference in commensals between these two groups. In the HIV-positive group, no difference was found between the pathogenetics and the commensals. However, in the HIV-negative group, the virulence of the pathogen was significantly higher than the commensals (P < 0.01).
CONCLUSIONSThese results indicate that oral candidiasis was not correlated with some predominant strains of Candida albicans with higher virulence in HIV-positive subjects.
Acquired Immunodeficiency Syndrome ; microbiology ; Candida albicans ; pathogenicity ; Candidiasis, Oral ; microbiology ; HIV Seropositivity ; microbiology ; Humans
4.Genetic diversity analysis of Penicillium marneffei isolated from AIDS patients in Guangdong, China using randomly amplified polymorphic DNA.
Ling-hua LI ; Feng-yu HU ; Wan-shan CHEN ; Wei-ping CAI ; Wei-nan SONG ; Yan-ling KUANG ; Xiao-ping TANG
Chinese Medical Journal 2012;125(5):823-827
BACKGROUNDPenicillium marneffei (P. marneffei) is an emerging pathogenic fungus that can cause invasive mycosis in patients with AIDS. The epidemiological features of P. marneffei infection in AIDS patients in Guangdong province remain unclear so far. This study aimed to investigate the genetic diversity within a population of 163 P. marneffei isolates obtained from AIDS patients and search for the dominant clinical strains in Guangdong province.
METHODSOne hundred and sixty-three P. marneffei isolates obtained from AIDS patients in Guangdong province during January 2004 and December 2009 were studied by randomly amplified polymorphic DNA (RAPD) using two random primers (H2 and H22). The degree of similarity between samples was calculated through similarity coefficients from RAPD fragment data and the dendrogram was assessed using the unweighted pair group method with arithmetic mean (UPGMA).
RESULTSTwo primers showed a high degree of discrimination and good stability. Primer H2 yielded eight different patterns (H2-1 to H2-8) among 163 isolates with the discriminatory power being 0.413. Primer H22 identified seven types (H22-1 to H22-7) among 163 isolates with the discriminatory power being 0.467. Genetic similarity coefficients based on RAPD data among 163 P. marneffei isolates ranged from 0.681 to 0.957, 61.96% of which were no less than 0.83. The discriminatory power of the two primers was 0.524. One hundred and sixty-three P. marneffei isolates were clustered into nine distinct groups (groups I to IX) at the similarity coefficient value of 0.83 and group I was the most common, including 101 strains (61.96%).
CONCLUSIONThe RAPD analyses could provide important information as to the degree of genetic diversity and the relationship among clinical P. marneffei isolates, revealing genetic polymorphism and dominant strains.
Acquired Immunodeficiency Syndrome ; microbiology ; Genetic Variation ; genetics ; Humans ; Penicillium ; classification ; genetics ; Random Amplified Polymorphic DNA Technique ; methods
5.Radiologic diagnosis for AIDS patients complicated with candidal esophagitis.
Gen-Dong YANG ; Pu-Xuan LU ; Jing QIN ; Shui-Teng LIU ; Neng-Yong ZHAN
Chinese Medical Journal 2011;124(7):965-967
BACKGROUNDCandidal esophagitis is the primary infection among all digestive tract opportunistic ones in acquired immunodeficiency syndrome (AIDS) cases. X-ray manifestation reports of it are still rare. This study aimed to conduct a retrospective analysis on the X-ray data of 6 AIDS cases complicated with candidal esophagitis, and to study the X-ray characteristics of it combined with the findings from gastroscopy.
METHODSAmong 6 cases in this series, all cases were confirmed by Shenzhen Center for Disease Control and Prevention (CDC) to be HIV positive and all of them had CD4 cell counts less than 150 × 10(6)/L. All cases underwent X-ray and gastroscopy, and mycelium were found in the mucous membrane of the esophagus.
RESULTSIn this series, the findings of the X-ray were as follows: (1) Affected areas: Four cases in the whole esophagus, 2 cases in the middle and lower part of esophagus; (2) Abnormal motivity: Six cases had decreased tension, loose walls, weakened peristalsis, decreased number of peristalsis waves and delayed emptying of barium; (3) Abnormal contour: Six cases had the sign of "decorative border" or "brush", two cases had narrowed canal; (4) Abnormal membrane and "cobblestone sign": Six cases had thickened membrane and "cobblestone sign" on the surface of the abnormal membrane. The hyperemia of mucosa was covered tightly with yellow-white pseudomembrane spots. This was in accordance with the small cobblestone-like filling defect found by X-ray.
CONCLUSIONSIf the AIDS cases have dysphagia, and X-ray shows that more than two sections of the esophagus are affected, with decreased motility, the walls in the sign of "brush" or "decorative edges", thickened membrane with "cobblestone sign", candidal esophagitis is highly possible.
Acquired Immunodeficiency Syndrome ; diagnosis ; diagnostic imaging ; Adult ; Candida ; pathogenicity ; Candidiasis ; diagnosis ; diagnostic imaging ; microbiology ; Esophagitis ; diagnosis ; diagnostic imaging ; microbiology ; Female ; Humans ; Male ; Radiography
6.Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals.
Jing LU ; Sai-Sai MA ; Wei-Ying ZHANG ; Jian-Ping DUAN
Journal of Zhejiang University. Science. B 2019;20(10):793-802
OBJECTIVE:
In this study, we investigated the changes in peripheral blood inflammatory factors and intestinal flora in acquired immune deficiency syndrome (AIDS) and human immunodeficiency virus (HIV)-positive individuals (AIDS/HIV patients), and explored the relationships among intestinal flora, peripheral blood inflammatory factors, and CD4+ T lymphocytes.
METHODS:
Thirty blood and stool samples from an AIDS group and a control group were collected. The levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay (ELISA), and the number of CD4+ T lymphocytes by a FACSCount automated instrument. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the messenger RNA (mRNA) levels of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus faecalis, and Enterococcus faecium. Correlations among intestinal flora, inflammatory factor levels, and CD4+ T lymphocyte values were evaluated using the Spearman correlation coefficient.
RESULTS:
The levels of TNF-α and IL-6 in the AIDS group were higher than those in the control group, while the number of CD4+ T lymphocytes was lower. The amounts of Bifidobacterium and Lactobacillus in the AIDS group were significantly lower than those in control group, while the amounts of E. coli, E. faecalis, and E. faecium were much higher. The amounts of Bifidobacterium and Lactobacillus were negatively correlated with the content of TNF-α and IL-6 and the CD4+ T lymphocyte count, while those correlations were reversed for E. coli, E. faecalis, and E. faecium.
CONCLUSIONS
The intestinal microbiota of AIDS/HIV patients were disordered, and there was a correlation between the amount of intestinal flora and the number of CD4+ T lymphocytes and the levels of TNF-α and IL-6.
Acquired Immunodeficiency Syndrome/microbiology*
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Adult
;
Aged
;
CD4 Lymphocyte Count
;
Female
;
Gastrointestinal Microbiome
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HIV Infections/microbiology*
;
Humans
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Interleukin-6/blood*
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Male
;
Middle Aged
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Tumor Necrosis Factor-alpha/blood*
7.Disseminated Histoplasmosis and Tuberculosis in a Patient with HIV Infection.
Hye Won JEONG ; Jang Wook SOHN ; Min Ja KIM ; Jung Woo CHOI ; Chul Hwan KIM ; Sang Ho CHOI ; Jeeyong KIM ; Yunjung CHO
Yonsei Medical Journal 2007;48(3):531-534
Histoplasmosis is a very rare disease in Korea. Clinical manifestations are very similar to those of tuberculosis. This is the first case report of combined disseminated histoplasmosis and tuberculosis in a patient with HIV infection in Korea. A 42-year-old Korean with Acquired Immunodeficiency Syndrome (AIDS) was diagnosed with tuberculosis. He had lived in Guatemala for the past five years. Upon diagnosis of disseminated tuberculosis with HIV infection, he was treated with anti-tuberculosis medications and anti-retroviral agents. Fever, weakness, hepatosplenomegaly and pancytopenia were persistent despite treatment. The patient's history of living in Guatemala caused us to seek opportunistic infectious organisms other than tuberculosis. Bone marrow aspiration and biopsy were performed and the result revealed numerous intracellular organisms consistent with Histoplasma capsulatum; therefore, the diagnosis of disseminated histoplasmosis was made.
AIDS-Related Opportunistic Infections/microbiology
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Acquired Immunodeficiency Syndrome/complications/pathology
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Adult
;
Bone Marrow/microbiology/pathology
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HIV Infections/*complications/drug therapy
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Histoplasma/isolation & purification
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Histoplasmosis/complications/*diagnosis/microbiology
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Humans
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Male
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Thorax/microbiology/pathology/radionuclide imaging
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Tuberculosis/complications/*diagnosis
8.Compare three methods to detect the Pneumocystis carinii in the bronchoalveolar wash sample of AIDS patients.
Liang ZHANG ; Xing-wang LI ; Bing SHEN ; Xiao-ying TENG ; Lei SUN ; Zhen-wei LANG ; Ping YANG ; Peng WANG
Chinese Journal of Pathology 2011;40(7):482-484
AIDS-Related Opportunistic Infections
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diagnosis
;
microbiology
;
Acquired Immunodeficiency Syndrome
;
diagnosis
;
microbiology
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Bronchoalveolar Lavage Fluid
;
microbiology
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Female
;
Humans
;
Immunohistochemistry
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Male
;
Methenamine
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Pneumocystis carinii
;
isolation & purification
;
Pneumonia, Pneumocystis
;
diagnosis
;
microbiology
;
Polymerase Chain Reaction
;
Silver Staining
;
methods
9.Helicobacter pylori infection in the gastric mucosa of patients with HIV/AIDS in different clinical stages.
Hong-Bin LUO ; Zhong-Wei HU ; Jia-Wei GUO
Journal of Southern Medical University 2009;29(7):1397-1399
OBJECTIVETo analyze Helicobacter pylori infection in the gastric mucosa of patients with HIV/AIDS in different clinical stages.
METHODSThis study involved 170 patients with HIV/AIDS and 34 HIV-negative patients. All the patients underwent upper endoscopy and antral gastric biopsy to determine the status of Helicobacter pylori infection using aniline red staining and rapid urease test. The patients with HIV/AIDS were stratified based on CD4(+)T lymphocyte counts and clinical setting into asymptomatic HIV infection (A1, A2) group, symptomatic HIV infection (B1, B2) group and AIDS (A3, B3, C1-3) group.
RESULTSThe prevalence of Helicobacter pylori infection in HIV/AIDS patients was 16.5% (28/170), and in the 3 groups classified, the infection rates were 23.4% (11/47), 14.0% (8/57), and 13.6% (9/66), respectively; the infection rate was 47.1% (16/34) in the control group. Helicobacter pylori infection rate in the gastric mucosa of the patients with HIV/AIDS in different clinical stages was significantly lower than that of the control group (P<0.05); the infection rates in symptomatic HIV-infected (B1, B2) group and AIDS (A3, B3, C1-3) group were significantly lower than that in asymptomatic HIV-infected (A1, A2) group (P<0.05).
CONCLUSIONThe low Helicobacter pylori infection rate in HIV/AIDS patients may result from severe immunodeficiency in the gastric mucosa.
AIDS-Related Opportunistic Infections ; microbiology ; pathology ; Acquired Immunodeficiency Syndrome ; microbiology ; pathology ; Adult ; Female ; Gastric Mucosa ; microbiology ; Gastroscopy ; HIV Infections ; microbiology ; pathology ; Helicobacter Infections ; epidemiology ; pathology ; Helicobacter pylori ; Humans ; Male ; Middle Aged
10.Study on genetic approach in the detection of Cryptosporidium parvum and Giardia lamblia in acquired immunodeficiency syndrome patients.
Si-qi LU ; Feng-yun WANG ; Ke ZHANG ; Lian-zhi XU
Chinese Journal of Epidemiology 2006;27(10):884-888
OBJECTIVETo establish genetic method in detecting Cryptosporidium parvum and Giardia lamblia which often coinfected with AIDS patients.
METHODSCryptosporidium oocysts and Giardia cysts were isolated and purified from fecal samples of the individuals infected with C. parvum and G. lamblia, respectively. Genomic DNAs were extracted. Two pairs of specific primers were designed or synthesized according to the 18S rRNA gene from C. parvum or the triose phosphate isomerase (tim ) gene from G. lamblia. Polymerase chain reaction(PCR) technique was used to amplify the DNA samples from the oocysts and the cysts, and those from the 6 control samples, including Schitosoma japonicum, Toxoplasma gondii , Entamoeba histolytica, Trichinella spiralis, Trichomonas vaginalis and human blood cells. DNA samples from 30 fecal samples of AIDS patients were detected with the same method.
RESULTSOne fragment of 500 bp was amplified with the primer of C. parvum, and the other one of 683 bp was amplified with the primer of G. lamblia. Twenty pg and 0.4 pg DNA of C. parvum and G. lamblia could be detected separately. The specificity of these two pairs of PCR primers was confirmed by the failure in the amplification of the control DNA samples. Out of 30 cases of AIDS patients, 7 showed C. parvum positive, while non Giardia was detected.
CONCLUSIONGenetic detection method for C. parvum and G. lamblia detection was established which was more sensitive and specific.
Acquired Immunodeficiency Syndrome ; microbiology ; Cryptosporidiosis ; diagnosis ; Cryptosporidium parvum ; genetics ; DNA, Bacterial ; Giardia lamblia ; genetics ; Giardiasis ; diagnosis ; Humans ; Polymerase Chain Reaction ; methods ; Sensitivity and Specificity