1.A case of community-acquired acinetobacter calcoaceticus pneumonia.
Byeong Moo YOO ; Byung Sung LIM ; Wan Young CHOI ; Dong Ho SHIN ; Sung Soo PARK ; Jung Hee LEE ; Tae Yul CHOI
Tuberculosis and Respiratory Diseases 1991;38(1):53-58
No abstract available.
Acinetobacter calcoaceticus*
;
Acinetobacter*
;
Pneumonia*
2.Evaluation of the Vitek 2 AST-N055 Card for the Susceptibility Testing of Acinetobacter baumannii Isolates to Amikacin.
Dong Jin PARK ; Wonkeun SONG ; Taek Kyung KIM ; Jae Seok KIM ; Han Sung KIM ; Kyu Man LEE
Korean Journal of Clinical Microbiology 2009;12(3):144-145
We collected 76 clinical isolates of Acinetobacter baumannii (amikacin MIC by Vitek 2 AST-N055 card: < or =2microgram/mL, 11 isolates; 4microgram/mL, 19 isolates; 8microgram/ mL, 17 isolates; 16microgram/mL, 27 isolates; and > or =64microgram/ mL, 2 isolates) from a university hospital and evaluated the Vitek 2 AST-N055 card vs the broth microdilution as a reference method for testing susceptibility to amikacin. Vitek 2 AST-N055 card yielded very major errors in 15 isolates (19.7%) and minor errors in 26 isolates (34.2%). Of the 15 isolates shown very major errors, 14 had Vitek 2 MICs ranging from 8 to 16microgram/mL. The results of our study suggest strongly that it is unreliable to test the amikacin susceptibility by Vitek 2 AST-N055 card of A. baumannii with the Vitek 2 MICs ranging from 8 to 16microgram/mL. In those cases, another susceptibility test, such as broth microdilution (BMD), should be performed to confirm the results.
Acinetobacter
;
Acinetobacter baumannii
;
Amikacin
3.A Case of Probable Community Acquired Acinetobacter baumannii Pneumonia.
Sang Hoon HAN ; Dong Jib NA ; Young Wook YOO ; Dong Gyu KIM ; You Ri MOON ; Kyoung Min MOON ; Yang Deok LEE ; Yong Seon CHO ; Min Soo HAN ; Hee Jung YOON
Tuberculosis and Respiratory Diseases 2007;63(3):273-277
No abstract available.
Acinetobacter baumannii*
;
Acinetobacter*
;
Pneumonia*
5.The emergence of Acinetobacter baumannii ST-6 multidrug resistant: A resilient biofilm producer, Malaysia
Jayakayatri Jeevajothi Nathan ; Mohd Nasir Mohd Desa ; Voon Chen Yong ; Parasakthi Navaratnam ; Navindra Kumari Palanisamy ; Eng Hwa Wong
Malaysian Journal of Microbiology 2016;12(4):265-269
Aims: The aim of this research is to explore the presence of multidrug-resistance (MDR) Acinetobacter baumannii
strains isolated from hospitalized patients in a tertiary-care center, Subang Jaya, Selangor, Malaysia and to compare
their genotypic and phenotypic characteristics.
Methodology and results: Clonal relationships were determined by multilocus sequence typing (MLST) and biofilm
forming ability was evaluated by using 2, 3 - bis (2 - methoxy - 4 - nitro - 5-sulfophenyl) - 5 - [(phenylamino) carbonyl] -
2H-tetrazolium hydroxide (XTT) reduction assay in microplates and Congo red agar method (CRA). Four virulence
genes coding for A. baumannii pilus usher-chaperone assembly protein, csuE gene; outer membrane protein, ompA
gene; biofilm poly-β-1, 6-Nacetylglucosamine (PNAG) synthesis protein, pgaA gene; and acinetobactin-mediated iron
acquisition protein, bauA gene were searched for in a collection of strains. Antimicrobial resistance against 11 antibiotics
were studied by broth microdilution method. Seventeen A. baumannii clinical strains were isolated and MLST showed
that the strains belonged to 5 distinct sequence types (STs), namely, ST-6, ST-265, ST-324, ST-325 and ST-432. Fiftythree
percent of the strains were resistant to 4 or more antibiotics. Twelve strains produced biofilm and out of them, 4
were strong biofilm producer, besides, these strong biofilm producers were MDR strains and belongs to ST-6. In
addition, all strains were ompA positive, biofilm producing strains were csuE and pgaA positive and only strong biofilm
producing strains were bauA positive.
Conclusion, significance and impact study: Our study demonstrates that the ST-6 strains in Malaysia could represent
MDR, capable of forming strong biofilm and possess csuE, ompA, pgaA and bauA genes, virulence characteristics that
probably help the bacteria to persist and cause infection.
Acinetobacter baumannii
6.Abrupt Increase in Rate of Imipenem Resistance in Acinetobacter baumannii Complex Strains Isolated from General Hospitals in Korea and Correlation With Carbapenem Administration During 2002–2013.
Young Ah KIM ; Yoon Soo PARK ; Taemi YOUK ; Hyukmin LEE ; Kyungwon LEE
Annals of Laboratory Medicine 2018;38(2):179-181
No abstract available.
Acinetobacter baumannii*
;
Acinetobacter*
;
Hospitals, General*
;
Imipenem*
;
Korea*
7.Colistin, Hot Potato for the Therapy of Carbapenem-resistant Acinetobacter baumannii infections
Journal of Korean Medical Science 2019;34(39):e265-
No abstract available.
Acinetobacter baumannii
;
Acinetobacter
;
Colistin
;
Solanum tuberosum
8.The status of multidrug resistance in ubiquitous and domination acinetobacter Spp caused nosocomial infections
Hieu Van Nguyen ; Anh Duc Dang ; Hanh Thuy Tran ; Binh Gia Nguyen
Journal of Preventive Medicine 2008;97(5):18-23
Background: Acinetobacter spp are present everywhere in the environment and cause many epidemics in tropical countries.\r\n', u'Objectives: This study aims to learn about the status of multidrug resistance in ubiquitous and domination acinetobacter Spp caused nosocomial infections. Subjects and method: A descriptive, epidemiologic cross-sectional study on 65 nosocomial Acinetobacter spp isolated from 244 patients hospitalized at the intensive-care units, Bach Mai hospital and burn patients from the National Burn Institute from April, 2007 to May, 2008. Results: Rates of A.baumannii were 70.8% of the isolates. Acinetobacter spp were isolated from patients in intensive-care units showed resistant to almost all commercially available antibiotics groups, among Penicillin ranged from 94.6 to 97.4%; beta- Lactam ranged from 80.5 to 90%; Cephems were 97.6%; Aminoglycosides group ranged from 62.5 to 100% and Quinolon were 100%. The isolates that were susceptible to Netilmycin was 35% and Imipenem was 34.1%. Acinetobacter spp were isolated from burn patients, which showed resistant to Penicillin was 86, 7%; beta- Lactam was 93, 3%; Aminoglyco- sides ranged from 25% to 87.5% and Quinolon was 81,3%. The isolates were susceptible to Netilmycin was 75% and Imipenem was 31.3%. \r\n', u'Conclusion: Analysis of risk factors may help the study of epidemiology Acinetobacter to prevent hospital infections and reduce the mortality rate. \r\n', u'
Acinetobacter spp
;
nosocomial infections
9.A Comparison of ID 32 GN System with Amplified Ribosomal DNA Restriction Analysis for Identification of Acinetobacter baumannii.
Myung Geun SHIN ; Soo Hyun KIM ; Je Chul LEE ; Duck CHO ; Seung Jung KEE ; Jong Hee SHIN ; Soon Pal SUH ; Dong Wook RYANG
The Korean Journal of Laboratory Medicine 2004;24(2):107-112
BACKGROUND: The glucose -acidifying genomic species 1, 2, 3 and 13 of the genus Acinetobacter are highly related genetically and may be difficult to differentiate by phenotypic identification schemes using biochemical tests. The aim of this study was to explore the brief restriction enzyme profiles of amplified ribosomal DNA restriction analysis (ARDRA) to identify medically important species of Acinetobacter. Using ARDRA analysis, we evaluated the ID 32 GN system (bioMerieux, Lyon, France) for the identification of A. baumannii (genospecies 2). METHODS: A collection of 78 A. baumannii stains initially identified by the ID 32 GN system was used to determine its accuracy by ARDRA analysis. ARDRA was performed with 10 different restriction enzymes, AluI (AGCT), CfoI (GCGC), HaeIII (GGCC), HinfI (GANTC), MboI (GATC), MspI (CCGG), NciI (CCGG), RsaI (GTAC), ScrFI (CCNGG) and TaqI (TCGA). RESULTS: The combination of restriction patterns obtained with respective enzymes AluI, CfoI and MboI allowed for the discriminatory value for the identification of medically important genospecies such as genospecies 2 (A. baumannii), 3 and 13. By comparing ARDRA results of the 78 strains previously identified by ID 32 GN system, we found the correlation rate between the two systems to be 88.5% (69/78). Nine strains were identified as Acinetobacter genospecies 13 by ARDRA. CONCLUSIONS: This result suggests that the ID 32 GN system may have difficulty in discriminating A. baumannii from genospecies 13. This revised ARDRA method gives a relatively rapid and definitive result for the identification of medically important genospecies of Acinetobacter.
Acinetobacter
;
Acinetobacter baumannii*
;
Coloring Agents
;
DNA, Ribosomal*
;
Glucose
10.Acinetobacter Infection in Neonatal Intensive Care Units(NICU).
Won Gyn BUM ; In Suk KIM ; Young Hee KIM ; Young Youn CHOI ; Jae Sook MA ; Tai Ju HWANG
Journal of the Korean Pediatric Society 1994;37(12):1669-1675
No abstract available.
Acinetobacter Infections*
;
Acinetobacter*
;
Infant, Newborn
;
Intensive Care, Neonatal*