1. In silico identification and characterization of common epitope-based peptide vaccine for Nipah and Hendra viruses
Chayan Kumar SAHA ; Md. SADDAM HOSSAIN ; Md. ASRAFUL JAHAN ; Abul Kalam AZAD ; Md. MAHBUB HASAN
Asian Pacific Journal of Tropical Medicine 2017;10(6):529-538
Objective To explore a common B- and T-cell epitope-based vaccine that can elicit an immune response against encephalitis causing genus Henipaviruses, Hendra virus (HeV) and Nipah virus (NiV). Methods Membrane proteins F, G and M of HeV and NiV were retrieved from the protein database and subjected to different bioinformatics tools to predict antigenic B-cell epitopes. Best B-cell epitopes were then analyzed to predict their T-cell antigenic potentiality. Antigenic B- and T-cell epitopes that shared maximum identity with HeV and NiV were selected. Stability of the selected epitopes was predicted. Finally, the selected epitopes were subjected to molecular docking simulation with HLA-DR to confirm their antigenic potentiality in silico. Results One epitope from G proteins, one from M proteins and none from F proteins were selected based on their antigenic potentiality. The epitope from the G proteins was stable whereas that from M was unstable. The M-epitope was made stable by adding flanking dipeptides. The 15-mer G-epitope (VDPLRVQWRNNSVIS) showed at least 66% identity with all NiV and HeV G protein sequences, while the 15-mer M-epitope (GKLEFRRNNAIAFKG) with the dipeptide flanking residues showed 73% identity with all NiV and HeV M protein sequences available in the database. Molecular docking simulation with most frequent MHC class-II (MHC II) and class-I (MHC I) molecules showed that these epitopes could bind within HLA binding grooves to elicit an immune response. Conclusions Data in our present study revealed the notion that the epitopes from G and M proteins might be the target for peptide-based subunit vaccine design against HeV and NiV. However, the biochemical analysis is necessary to experimentally validate the interaction of epitopes individually with the MHC molecules through elucidation of immunity induction.
3.A Comprehensive View on the Progress of Organoid Research with an Emphasis on its Relevance to Disease Characterization.
Chandra KISHORE ; Vaishali JI ; Saurav MALLIK ; Ayan MUKHERJI ; Namrata TOMAR ; Kumar Pati SOUMEN ; Ai Min LI ; Sinthia Roy BANERJEE ; Soumadip GHOSH ; Raza Ali NAQVI
Biomedical and Environmental Sciences 2023;36(10):959-971