1. Effect of HIF-2α on regulating CDCP1 to promote hepatocellular carcinoma metastasis
Manqing CAO ; Junrong GAO ; Jiafei HUANG ; Abin YOU ; Zhigui GUO ; Hongyuan ZHOU ; Feng FANG ; Wei ZHANG ; Xiaolin ZHU ; Ti ZHANG
Chinese Journal of Oncology 2017;39(1):18-23
Objective:
To investigate the effect of hypoxia inducible factor 2α (HIF-2α) on regulating CUB domain-containing protein 1 (CDCP1) and its role in hepatocellular carcinoma metastasis.
Methods:
HIF-2α-knocked down and HIF-2α-stably overexpressing cells (MHCC97H) were prepared by small interfering RNA (siRNA) and lentivirus transfection, respectively. The expression of CDCP1 protein and mRNA in the above cells was detected by western blot and real-time PCR. The effect of HIF-2α on cell invasion ability was determined by Transwell assay. Furthermore, immunohistochemical staining was performed to detect the expression of CDCP1 in human HCC tissue samples.
Results:
Both HIF-2α and CDCP1 were induced under hypoxic conditions. The activation of CDCP1 under hypoxic conditions was dependent on the expression of HIF-2α.When HIF-2α was overexpressed, the mRNA level of CDCP1 was greatly upregulated (5.92±0.28,