1.Genomic Epidemiology of SARS-CoV-2 in Pakistan
Song SHUHUI ; Li CUIPING ; Kang LU ; Tian DONGMEI ; Badar NAZISH ; Ma WENTAI ; Zhao SHILEI ; Jiang XUAN ; Wang CHUN ; Sun YONGQIAO ; Li WENJIE ; Lei MENG ; Li SHUANGLI ; Qi QIUHUI ; Ikram AAMER ; Salman MUHAMMAD ; Umair MASSAB ; Shireen HUMA ; Batool FATIMA ; Zhang BING ; Chen HUA ; Yang YUN-GUI ; Abbasi Ali AMIR ; Li MINGKUN ; Xue YONGBIAO ; Bao YIMING
Genomics, Proteomics & Bioinformatics 2021;19(5):727-740
COVID-19 has swept globally and Pakistan is no exception.To investigate the initial introductions and transmissions of the SARS-CoV-2 in Pakistan,we performed the largest genomic epidemiology study of COVID-19 in Pakistan and generated 150 complete SARS-CoV-2 genome sequences from samples collected from March 16 to June 1,2020.We identified a total of 347 mutated positions,31 of which were over-represented in Pakistan.Meanwhile,we found over 1000 intra-host single-nucleotide variants(iSNVs).Several of them occurred concurrently,indicating possible interactions among them or coevolution.Some of the high-frequency iSNVs in Pakistan were not observed in the global population,suggesting strong purifying selections.The genomic epidemiology revealed five distinctive spreading clusters.The largest cluster consisted of 74 viruses which were derived from different geographic locations of Pakistan and formed a deep hierarchical structure,indicating an extensive and persistent nation-wide transmission of the virus that was probably attributed to a signature mutation(G8371T in ORF 1ab)of this cluster.Further-more,28 putative international introductions were identified,several of which are consistent with the epidemiological investigations.In all,this study has inferred the possible pathways of introduc-tions and transmissions of SARS-CoV-2 in Pakistan,which could aid ongoing and future viral surveillance and COVID-19 control.
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