1.Survey of Flea Infestation in Dogs in Different Geographical Regions of Iran.
Mosa TAVASSOLI ; Amir AHMADI ; Abbas IMANI ; Emad AHMADIARA ; Shahram JAVADI ; Mojtaba HADIAN
The Korean Journal of Parasitology 2010;48(2):145-149
Medically important arthropods, including fleas, play an important role in causing clinical disorders and disease in man and domestic animals. This study was conducted to determine the seasonal flea infestations for domestic dogs from different geographic regions of Iran. A total of 407 fleas, belonging to 5 different species, were recovered from 83 domestic dogs from 3 regions. There was a distinctive pattern of species distribution and infestations with the highest infestation rates observed in a temperate climate and higher rainfall. Additionally, fleas were observed over all seasons, except February and March, with the highest infestation rate observed in August (24.7%) and the lowest rate in January (1.7%). They also parasitize dogs with a different spectrum of species. The cat flea, Ctenocephalides felis (67.5%), exhibited the highest prevalence among all flea species found on dogs. Thus, climatic conditions and seasonal patterns impact on flea infestation and must be considered in developing control programs.
2.Evaluation of an anal sac adenocarcinoma tumor in a Spitz dog
Javanbakht Javad ; Tavassoli Abbas ; Sabbagh Atefeh ; Hassan Aghamohammmad Mehdy ; Samakkhah Alian Shohreh ; Shafiee Radmehr ; Lakzian Ali ; Ghalee Rahmani Vahideh ; Gharebagh Shoja Sonia
Asian Pacific Journal of Tropical Biomedicine 2013;(1):74-78
A 9-year-old emasculated male Spitz with tenesmus and constipation had a subcutaneous mass at the left ventral aspect of the anus with history of polyuria and polydipsia. A complete blood cell count, serum biochemistry panel, and urinalysis (cystocentesis sample) were evaluated. Abnormalities in the serum biochemistry panel included a mildly elevated serum cholesterol concentration (7.28 mmol/L; reference interval, 2.70-5.94 mmol/L), increased serum alkaline phosphatase activity (184 U/L; reference interval, 9-90 U/L), alanine transaminase (122 U/L;reference interval, 5-60 U/L) activity and aspartate aminotransferase (80 U/L; reference interval, 5-55 U/L) activity, severe increased total calcium concentration (16.3 mg/dL; reference interval, 8.2-12.4 mg/dL or 9.3-11.4 mg/dL), and decreased total calcium concentration (3.4 mg/dL, reference interval, 2.5-5.6mg/dL). Furthermore, testing revealed an increased intact parathyroid hormone concentration (38.6 pmol/L; reference interval, 3-17 pmol/L). On cytologic and histopathologic examinations, various types of cells were observed. Most of the cells were oval to polygonal and had elliptical or elongate nuclei and a moderate amount of pale to basophilic cytoplasm. The remaining cells had round to oval nuclei and pale to basophilic cytoplasm. Cells of both types were loosely adhered to each other and were arranged in rosette-like structures. Both neoplastic cell types had fine homogenous chromatin and either a small indistinct nucleolus or no visible nucleolus. Mild anisokaryosis and anisocytosis were observed. Histologically, the mass consists of glandular structures formed by cuboidal cells admixed with bundles of spindle cells. Based on location and histologic features, the final diagnosis was adenocarcinoma of the apocrine gland of the anal sac, which should be included as a cytologic differential diagnosis when spindle cells and typical epithelial cells are observed in masses in the region of the anal sac of dogs.
3.An overall assessment of circumanal gland adenoma in a terrier mix breed dog
Javanbakht Javad ; Tavassoli Abbas ; Sasani Farhang ; Sabbagh Atefeh ; Hassan Aghamohamad Mehdi ; Samakkhah Alian Shohreh ; Shafiee Radmehr ; Jani Meysam ; Alimohammadi Samad ; Samani Reza ; Barati Fardin ; Ghalee Rahmani Vahideh
Asian Pacific Journal of Tropical Biomedicine 2013;(7):580-583
In September 2012, a 10-year-old, intact male, terrier mix breed dog was evaluated because of multiple, 0.5 to 1.2 cm in diameter, round, intradermal nodules around the anus. It had surgery to excise a firm, painful swelling in the left ventrolateral perianal region and the excision part was observed under light microscopy. The mass spreading in to sub acute was of left hind leg out from the ventro-lateral of anus, 1.2 cmí1 cm/ 0 cmí0.5 cm in size and 125 g in weight. A complete blood cell count, serum biochemistry panel, and urinalysis (cystocentesis sample) were evaluated. Significant laboratory data demonstrated microcytic anemia (hemoglobin of 6.4 mg/dL) and normal coagulation times. No remarkable abnormalities were found in the complete blood count and an ionized calcium of 1.91 mmol/L (reference range, 1.1-1.3 mmol/L) was confirmed hypercalcemia. On cytologic and histopathologic examinations, evaluation of the aspirate revealed a prominent population of round-to-polygonal nucleated cells arranged as cohesive groups with isolated individual cells. A mild degree of anisocytosis and anisokaryosis was observed. In addition, smaller reserve type cells, with darker cytoplasm and a higher nucleocytoplasmic ratio. The adenomas generally retain the lobular architecture, but some may contain focal areas of cellular pleomorphism. These changes may suggest malignant transformation and have led to discordant interpretations, the well-developed stroma surrounding the lobules and hepatoid cells was noted. Ulceration, hemorrhage, necrosis and secondary infection with inflammatory cell infiltrates are common. These cytology and histopathology features are consistent with hepatoid gland adenoma.
4.Evaluation of an anal sac adenocarcinoma tumor in a Spitz dog.
Javad JAVANBAKHT ; Abbas TAVASSOLI ; Atefeh SABBAGH ; Mehdy Aghamohammmad HASSAN ; Shohreh Alian SAMAKKHAH ; Radmehr SHAFIEE ; Ali LAKZIAN ; Vahideh Rahmani GHALEE ; Sonia Shoja GHAREBAGH
Asian Pacific Journal of Tropical Biomedicine 2013;3(1):74-78
A 9-year-old emasculated male Spitz with tenesmus and constipation had a subcutaneous mass at the left ventral aspect of the anus with history of polyuria and polydipsia. A complete blood cell count, serum biochemistry panel, and urinalysis (cystocentesis sample) were evaluated. Abnormalities in the serum biochemistry panel included a mildly elevated serum cholesterol concentration (7.28 mmol/L; reference interval, 2.70-5.94 mmol/L), increased serum alkaline phosphatase activity (184 U/L; reference interval, 9-90 U/L), alanine transaminase (122 U/L; reference interval, 5-60 U/L) activity and aspartate aminotransferase (80 U/L; reference interval, 5-55 U/L) activity, severe increased total calcium concentration (16.3 mg/dL; reference interval, 8.2-12.4 mg/dL or 9.3-11.4 mg/dL), and decreased total calcium concentration (3.4 mg/dL, reference interval, 2.5-5.6mg/dL). Furthermore, testing revealed an increased intact parathyroid hormone concentration (38.6 pmol/L; reference interval, 3-17 pmol/L). On cytologic and histopathologic examinations, various types of cells were observed. Most of the cells were oval to polygonal and had elliptical or elongate nuclei and a moderate amount of pale to basophilic cytoplasm. The remaining cells had round to oval nuclei and pale to basophilic cytoplasm. Cells of both types were loosely adhered to each other and were arranged in rosette-like structures. Both neoplastic cell types had fine homogenous chromatin and either a small indistinct nucleolus or no visible nucleolus. Mild anisokaryosis and anisocytosis were observed. Histologically, the mass consists of glandular structures formed by cuboidal cells admixed with bundles of spindle cells. Based on location and histologic features, the final diagnosis was adenocarcinoma of the apocrine gland of the anal sac, which should be included as a cytologic differential diagnosis when spindle cells and typical epithelial cells are observed in masses in the region of the anal sac of dogs.
Adenocarcinoma
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diagnosis
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pathology
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surgery
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veterinary
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Anal Gland Neoplasms
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diagnosis
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diagnostic imaging
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pathology
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surgery
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Anal Sacs
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pathology
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surgery
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Animals
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Diagnosis, Differential
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Dog Diseases
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diagnosis
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pathology
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surgery
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Dogs
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Male
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Species Specificity
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Treatment Outcome
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Ultrasonography
5.The insecticidal effect of diatomaceous earth against adults and nymphs of Blattella germanica.
Seyyed Akbar HOSSEINI ; Sahar BAZRAFKAN ; Hassan VATANDOOST ; Mohammad Reza ABAEI ; Mussa Soleimani AHMADI ; Maryam TAVASSOLI ; Mansoreh SHAYEGHI
Asian Pacific Journal of Tropical Biomedicine 2014;4(Suppl 1):S228-32
OBJECTIVETo evaluate the insecticidal effect of diatomaceous earth (DE) against adults and nymphs of Blattella germanica.
METHODSThis cross sectional study has been done on the laboratory strain of German cockroaches. Two stages, nymph and adult, were exposed to six dose rates of the DE, 2.5, 5, 10, 15, 20 and 25 g/m(2), at 24, 48 and 72 h exposure period. Mortality (number of dead cockroaches) was assessed after 24 h. Other exposed specimens were transferred to the beakers contained food and water for counting the retard mortality rate after 1 week.
RESULTSIncreasing in dose rates of DE increased mortality rate, so that the lowest and highest mortality rates were observed in 2.5 and 25 g/m(2), respectively. The results of the statistical analysis showed no significant difference in the lethality of 50% of DE plus water on the German cockroach nymphs.
CONCLUSIONSDue to the resistance of German cockroach against organochloride, organophosphorus, carbamate and pyrethriodes insecticides, it is suggested to use DE for insect's control.