Background and objective This study was conducted to evaluate the effects of the accumulation-associated protein (Aap) gene and transform growth factor-beta 1 (TGF-β1) on the bioiflm formation of lung cancer-related Staphylococcus epidermidis (SE). Methods Species identification was performed to isolate SE strains from clinically im-planted materials in lung cancer patients. Stable genetic aggregated proteins, which are associated with negative and positive isolates, were obtained. hTe bioiflm-formation ability of the SE Aap gene was determined by PCR. Density gradient method was used to extract peripheral blood mononuclear cells from patients with non-small cell lung cancer. Atfer 30 h, these cells were co-cultured with A549 at different TGF-β1 concentrations. hTe supernatant was then combined with SE Aap+and SE Aap-strains and co-cultured with a medical silicone rubber. A semi-quantitative adhesion test was performed for each bacterial bioiflm formation. Scanning electron microscopy was also conducted to observe the microcosmic condition of this material on the bacterial bioiflm surface. Results hTe Aap gene was closely related to SE bioiflm formation. At 10 ng/mL, 20 ng/mL, and 40 ng/mL, SE Aap+bioiflm on the medical silicone rubber surface was thicker in the TGF-β1 group than in the control group. No signiifcant differences were found between TGF-β1 groups. For the SE Aap-strains, no evident bioiflm was formed in TGF-β1 and control groups. Conclusion In plant material-related infection of lung cancer patients, SE Aap+strain easily forms bioiflm. Furthermore, TGF-β1 was conducive for the bioiflm formation of SE Aap+strains.