AIM:To develop a RP-HPLC method for simultaneously determining three major compounds in Qingre Mingmu Tea(Semen Cassiae,Flos crysanthemi,etc),namely emodin,chrysophanol and physcion with UV detection wavelength at 254 nm. METHODS: The column was Diamonsil C_(18)(250 mm?4.6 mm i.d,5 ?m).The separation was carried out with a gradient program.The mobile phase was acetonitrile-0.1% phosphoric acid at a flow rate of 1.0 mL/min. RESULTS: The standard curve was rectilinear in the range of 5.335213.4 ng(r=(0.999 8)) for emodin,9.075-363.0 ng(r=0.999 8) for chrysophanol,and 4.688-187.5 ng(r=1.000 0) for physcion,respectively.The recoveries of the markers listed above were 96.0%,106.5% and 98.1%,respectively. CONCLUSION: The method is simple,rapid and suitable for the determination of three compounds in Qingre(Mingmu) Tea.

Objective To observe the changes of serum adiponectin and free fatty acids in different glycometabolic states and to ex- plore their roles in the development and progression of diabetes. Methods The serum adiponectin and free fatty acids in 25 subjects with normal glucose (NGT) ,27 subjects with impaired glucose tolerance (IGT) and 25 subjects with diabetes (T2DM) , and the effects of these factors on insulin resistance (HOMA - IR) and islet ficell function(HBCI) were analyzed respectively. Results (1) Serum levels of adiponectin was lower in T2DM and IGT as compared with NGT and the level of FFA was increased in T2DM and IGT compared with NGT and the differences were significance (P

Objective: To review the influence of various processing methods on the content of baicalin in radix Scutellariae and its preparations. Method: The content of baicalin was determined by HPLC. Results: The content of baicalin decreased evidently when the brocessing method was in appropriate. Conclusion: Improper processing method can decrease the content of baicalin, and frying and carbonizing destroyed baicalin seriously.

Objective:To evaluate the safety of one-lung ventilation and artificial hydrothorax to aid HIFU treatment of liver carcinoma located in distal end of diaphragm and edge of liver and to observe the related indices in the treatment.Methods:Twelve patients with liver carcinoma received general anesthesia using double lumen endobronchial tube bi-chamber trachea cannula.Saline was injected into right thoracic cavity to turn artificial pleural fluid.During HIFU treatment,left lung had a ventilation with 100% FiO_2.Before cannula(value of Base);one lung was ventilated(DLV) for 15 mins,30 mins,60mins,120mins,then double lungs received ventilation again for 30mins(DLV'30).And blood gas was analyzed separately.Vital monitoring machine kept observing ECG,HR,MAP,S_pO_2,and PETCO2.One day and seven days after HIFU,X-ray was made to evaluate the outcome of hydrothorax.Results:All the patients were steady in blood gas and hemodynamic indices at the period of one-lung ventilation with artificial hydrothorax.The indices of PO_2 were higher during one-or two-lung ventilation than Base (P

Student leaders are the important work force which the college student management and education in the process to rely on.They are not only the bridge and link between schools,teachers and ordinary students,but also the reserve of schools and social management cadres.Grasping and analyzing their basic conditions,the growing demand and group identity is significant.In this paper,the group identity of the student leaders has been sorted out,and their needs and deficiencies are understood through research and discussion about their positioning in the self-awareness,growth and success.And by drawing on the experience of student affairs management in the United States such as ＂student services＂,＂student development ＂,＂student learning＂,we put forward some suggestions in team-building and personnel training of the current student leaders etc..

Crohn's disease (CD) complications include intestinal complications,parenteral complications,and systemic manifestations,of which intestinal complications are the most common.These complications result in serious damage to the quality of life,the risk of life-threatening may also appear.How to deal with these complications has become a major challenge for clinicians.CD requires a multidisciplinary treatment team,treatment complications versa.This review summarized the treatments of CDcommon complications.

There are some problems in the establishment and development of the organ procurement organiza -tion ( OPO) , such as a hospital public welfare nature questioned;OPO mechanism is not perfect , the unified mod-el was not established;the staff structure as reasonable etc .Through the analysis and summarization of the above problems, suggestions for implementing appropriate compensation and maximum in the future practical use princi-ple, and ethics training to the staff , in order to promote the development of our body organ donation .

Objective To investigate the impact of triptolide (TP) on proliferation and apoptosis of imatanib resistant CML cell (K562/G01) and its regulating effect on Wnt/β3-catenin signal pathway.Methods A series of 10,20,40,and 80 nmol/L of triptolide were used in CML cells K562/G01 for 12,24,and 48 hours.The cell proliferation was detected with methyl thiazolyl tetrazolium (MTT) test.The apoptosis was assessed with flow cytometry (FCM).The mRNA expressions of breakpoint cluster region-c-abl (BCR-ABL),β-catenin and its down-stream targets Lef-1,and cyclinD1 were analyzed with real-time quantitative polymerase chain reaction (RT-PCR),respectively.Results Triptolide significantly inhibited K562/G01 cell growth ability and induced apoptosis in a dose-dependent manner.Mter being treated with 20,40 nmol/L TP for 24 hours,the cell growth inhibition rates were (22.62 ± 1.33) ％,and (51.41 ±1.39) ％,respectively.The late apuptosis rates were (6.91 ± 0.14) ％,and (7.64 ± 0.47) ％,respectively.Meanwhile,PCR data showed that the mRNA levels of BCR-ABL were decreased,compared to the control group,the mRNA levels of β-catenin,Lef-1,and cyclinD1 were also decreased obviously after treatment.Conclusions Our data indicated that the triptolide could inhibit the proliferation and induce the apoptosis of K562/G01,and the mechanism might be related to the blockade of Wnt/β-catenin signal pathway.

OBJECTIVE： To prepare pefloxacin mesylate eye drops.METHODS： The content of pefloxacin was determined with UV－ spectrophotometry.The therapeutic effect of the eye drops was observed in 170 out－ patients.RESULTS： The linearity range was 2～ 14μ g/ml with a regression equation of A=0.1 018C and r=0.9 999(n=7).The average recovery was 99.64％ with a RSD of 0.43％ .After treatment,150 out－ patients recovered,16 out－ patients improved and 4 out－ patients had no efficiency with a total effective rate of 97.6％ .CONCLUSION： The eye drops has definite therapeutic effect.The UV－ spectrophotometry method is simple,rapid and accurate in determination of the content and no special reagent is needed.

Objective To observe and evaluate the effect of glycolic acid peels in the treatment of acne and scars,and to approach the indications,process and security.Methods 34 patients with acne were chosen.Glycolic acid of 20 ％,35 ％,50 ％,and 70 ％ concentration was used for chemical peeling therapy for 5 times.The concentration of glycolic acid was increased every three weeks.The efficacy was evaluated by comparison of the lesion counts before and after the course.Patients were assigned to fulfil the questionnaire to assess the follicular orifice,secretion of sebaceous glands,smooths,whiteness,scar,influence to living and satisfaction.Results 34 patients completed the course.4 patients were cured,15 patients were greatly improved,12 patients were improved,and 3 patients had no effectiveness.The efficacy rate was 91.18 ％.After treatment,73.53 ％ of the patients showed improvement in follicular orifice,55.88 ％ of the patients showed that secretion of sebaceous glands was decreased,82.35 ％ of the patients had improvement in smoothness,61.77 ％ of the patients had improvement in whiteness,and 67.65 ％ of the patients had decrease in scar formation.Conclusions Glycolic acid peeling is proved an effective,well accepted,and safe treatment for mild to moderate acne.The follicular orifice,secretion of sebaceous glands,skin smoothness,whiteness and scars could be also improved after treatment.