Objective　To investigate the effects of DiBaiRen decoction on the proliferation of human keratinocytes and its in vitro anti-oxidation role .　Methods　Human keratinocytes were cultured in vitro in the media containing gradient diluted DiBaiRen decoction. The cell proliferation rates at different culture times were recorded. Cellular oxidation injury inflicted by superoxide anion and hydroxyl anion was produced by adding hypoxanthine-xanthine oxidase and hydrogen peroxide to the culture media. MTT colorimetry was employed to determine cellular activity.The antagonistic effects of DiBaiRen decoction on cellular oxidation injury were analyzed.　Results　In the case that DiBaiRen decoction was added to the culture in concentrations of 30ml/L to 100ml/L, there exhibited obvious accelerated proliferation of the keratinocytes,with evident shortening of the cell fusion time. In addition, the oxidation injury of cultured keratinocytes caused by hypoxanthine-xanthine oxidase and hydrogen peroxide was ameliorated or eliminated by the addition of DiBaiRen decoction,and the effects were dose-dependent.　Conclusion　DiBaiRen decoction might possess anti-oxidation effects and could promote the in vitro proliferation of cultured human keratinocytes,and it might be beneficial in wound protection and epithelization.