1.Eukaryotic elongation factor 2 kinase and cancer.
Gen-zhu WANG ; Xin QI ; Jing LI
Acta Pharmaceutica Sinica 2015;50(7):808-813
Eukaryotic elongation factor 2 kinase (eEF2K) is well known as a Ca2+/calmodulin (CaM)-dependent kinase. eEF2K catalyzes the phosphorylation of eEF2 and subsequently inactivates eEF2 by impairing its ability to bind to the ribosome, thereby negatively modulates protein synthesis. The high expression of eEF2K has been found recently in several types of malignancies. As participating in the progress of tumor, eEF2K emerges a potential target for future cancer therapy. The relationship between eEF2K and tumor, and the latest progress of eEF2K inhibitors were summarized in this article.
Elongation Factor 2 Kinase
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antagonists & inhibitors
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metabolism
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Humans
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Neoplasms
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metabolism
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Peptide Elongation Factor 2
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metabolism
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Phosphorylation
2.Recent advances in the study of mechanism of APOBEC3G against virus.
Yan-Ping ZHU ; Jian-Dong JIANG ; Zong-Gen PENG
Acta Pharmaceutica Sinica 2014;49(1):30-36
APOBEC3 is a class of cytidine deaminase, which is considered as a new member of the innate immune system, and APOBEC3G belongs to this family. The research about APOBEC3G is a new direction of innate immune defense mechanism against virus. APOBEC3G has the restrictive activity on many viral replications, which deaminates dC to dU in the viral genome and then induces extensive hypermutation. APOBEC3G can also interrupt viral replication at several phases such as reverse transcription, replication, nucleocapsid and so on by non-deamination mechanisms. However, virus can encode viral proteins to counteract the restriction activity of APOBEC3G. Elucidation of the antagonistic interaction between APOBEC3G and the virus will be contributed to development of new antiviral drugs in the future.
APOBEC-3G Deaminase
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Animals
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Cytidine Deaminase
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genetics
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metabolism
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DNA Replication
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Deamination
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HIV-1
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physiology
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Hepacivirus
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genetics
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physiology
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Hepatitis B virus
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genetics
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physiology
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Humans
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Paramyxoviridae
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genetics
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physiology
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Retroviridae
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physiology
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Virus Replication
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vif Gene Products, Human Immunodeficiency Virus
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metabolism
3.Change of metabolism of serum lipids in patients with mild cognitive impairment
hong-lian, ZHU ; zhi-hong, PAN ; gen-fa, WANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(05):-
Objective To study the change of metabolism of serum lipids in patients with mild cognitive impairment(MCI). Methods The serum levels of total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL) and high density lipoprotein(HDL) were measured in 60 patients with MCI and 100 age-matched normal controls. ResultsThe serum levels of TC,TG and LDL were significantly higher and HDL significantly lower in patients with MCI than in normal controls(P
4.Development of swine Salmonella vaccine
Shanshan ZHU ; Yingying ZHOU ; Shizhong GEN ; Zhiming PAN ; Xinan JIAO
Chinese Journal of Zoonoses 2017;33(6):548-552
Salmonella is an important zoonosis.China is the largest pork consumer.Contaminated pork is the main source of Salmonella disease at home and abroad.There are many swine Salmonella vaccine in use in Europe.Now the current swine Salmonella vaccine is a live attenuated vaccine,Salmonella can reduce colonization in pigs,and can induce immune response efficiently after the two immunizations.The control effect is poor,at this stage of swine vaccine cross protection research,type of Salmonella and vaccine antigen are not the same.This paper reviews the research progress of swine Salmonella vaccine.
5.Relationship between NF-κB p65 activation and k-ras gene mutation in colorectal cancer
Ling CHEN ; Yunbin YE ; Yanping CHEN ; Gen LIN ; Weifeng ZHU
Cancer Research and Clinic 2013;25(8):513-515,526
Objective To investigate the corresponds between NF-κB p65 activation and k-ras mutations.Methods NF-κB p65 activation was analyzed by immunochemistry in 167 colorectal cancer specimens in which the k-ras mutation status was confirmed.Resluts Among 167 colorectal cancer specimens screened,59 (35.3 %) had k-ras mutations and 63 (37.7 %) had NF-κB p65 activation.k-ras mutations and NF-κB p65 nuclear expression were no significant difference in different sex,age,ECOG score,pathological types,degrees of pathological differentiation,TNM stage,and metastases on lymph nodes (P> 0.05).The positive nuclear expression rate of NF-κB p65 was 50.8 % (30/59) in specimens with k-ras mutations and 30.6 % (33/108) in specimens with wild type k-ras.There was obviously statistical difference between them (P =0.010).Conclusion NF-κB p65 activation in coloretal cancer was associated with k-ras mutations.
6.Glycosides from Machilus wangchiana.
Wei SHENG ; Wen-dong XU ; Cheng-gen ZHU ; Yong-chun YANG ; Jian-gong SHI
China Journal of Chinese Materia Medica 2015;40(6):1102-1107
Ten glycosidic compounds were isolated from an ethanol extract of Machilus wangchiana by a combination of various chromatographic techniques including column chromatography over silica gel and Sephadex LH-20 and reversed-phase flash chromatography and HPLC. Their structures were identified by spectroscopic data analysis (IR, MS, and NMR) as icariside B1 (1), boscialin-3-O-β-D-glucopyranoside (2), pisumionoside (3), isolariciresinol-9'-O-β-D-xylopyranoside (4), 5'-methoxyisolariciresinol-9'-O-β-D-xylopyranoside (5), lyoniresinol-9'-O-β-D-xylopyranoside (6), (E) -4-hydroxyphenylprop-7-ene 4-O-β-D-glucopyranoside (7), (E) - 4-hydroxy-3-methoxyphenylprop-7-ene 4-O-α-L-rhamnopyranosyl-(1 --> 6) -β-D-glucopyranoside (8), 4-hydroxy-3-methoxyphenylprop-8-ene 4-O-β-D-xylopyraosyl-(1 --> 6) -β-D-glucopyranoside (9), and 4-hydroxy-3,5-dimethoxyphenylprop-8-ene 4-O-α-L-rhamnpyranosyl-(1 --> 6)-β-D- glucopyranoside (10), respectively.
Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Glycosides
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chemistry
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isolation & purification
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Lauraceae
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chemistry
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Spectrometry, Mass, Electrospray Ionization
7.Effect of different altitudes on the morphology of pituitary ACTH cells in rats
Juxiang HE ; Paili GEN ; Yuzhang TIAN ; Hongfu WU ; Haihong ZHU ; Min ZHAO
Journal of Medical Postgraduates 2003;0(05):-
Objective: To observe the morphologic changes of pituitary adrenocorticotrophic hormone (ACTH) cells in Wistar rats at different altitudes, and clarify the mechanism of stress reaction to hypoxia in ACTH cells. Methods: Wistar rats were divided into three groups and moved to different altitudes (1700 m, 3100 m, 4050 m). After 12 days, changes of ACTH cells were observed by using immunohistochemisty, image analysis and electron microscopy. Results:The ratio (R) of immunoreactive cell area to scanned area and mean optical density (A) increased at higher altitude with statistically different R values between groups of 1700 m and 4050 m (P
8.Damage control surgery directed surgical treatment of Crohn disease.
Ning LI ; Wei-ming ZHU ; Lu-gen ZUO
Chinese Journal of Gastrointestinal Surgery 2013;16(4):308-310
Damage control surgery (DCS) has been widely used in the management of surgical patients. Crohn disease (CD) patients requiring surgery are usually severe and associated with high surgical risk, while the concept of DCS has not gained adequate attention in surgery for CD. Surgery is indicated in patients with CD to control symptoms, therefore major surgery should not be performed when the general health of the patients is not satisfactory. Use of DCS to guide surgery can reduce risk of treatment and improve clinical outcome The review is to discuss the necessity, objective, and methods of damage control surgery in the surgical treatment of Crohn disease.
Crohn Disease
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surgery
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Humans
9.Research and development of a clinical laboratory information system.
Da-gan YANG ; Gen-yun XU ; Yang-jun ZHU
Chinese Journal of Medical Instrumentation 2005;29(1):23-26
A clinical laboratory information system consists of two parts--the information system and the management system. Its development is based on scientific and rational lab-workflow, consulting the international standard HL7 Protocol, and combined with barcode technique and instrument communication. The information system mainly manages the data which come from the whole lab testing process while the management system is dominating the lab office work and management decisions.
Automatic Data Processing
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Clinical Laboratory Information Systems
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standards
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Computer Communication Networks
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Databases as Topic
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Management Information Systems
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Software Design
10.Research on the illuminance characteristics of LED surguical luminaire.
Chinese Journal of Medical Instrumentation 2009;33(3):183-187
The illuminance characteristics of LED (Lighting Emitting Diode) Surgical Luminaire is researched in this paper from the aspects of the LED Single-tube illumination analysis, LED arrays distribution and lighting design. The facula distribution characteristics of the LED prototype and multi-facet entirety reflection Surgical Luminaire is tested and compared according to the standards. The results of experimental show that LED prototype can fully meet the surgical illumination requirements, and the LED's own characteristics and further development will greatly broaden the space of medical lighting.
Equipment Design
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Light
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Lighting
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instrumentation
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Surgical Equipment