OBJECTIVE:To evaluate therapeutic efficacy and safety of chemotherapy combined with Xiaoaiping injection in the treatment of elderly patients with advanced gastric cancer. METHODS:60 elderly patients with advanced gastric cancer were collected from our department during 2012-2014,and then divided into union group and single group with 30 cases in each group. Single group was given irinotecan(CPT-11)150 mg/m2 added into 0.9%Sodium Chloride injection,and union group was addition-ally given Xiaoaiping injection 80 ml,qd,on the basis of single group. A treatment course lasted for 14 days,and both groups re-ceived 4 courses of treatment. The tumor markers,disease single rate,progression-free survival (PFS) and the incidence of ADR were compared between 2 groups. RESULTS:After treatment,the levels of CEA,CA199 and CA242 in 2 groups were significant-ly lower than before;the union group was significantly lower than the single group,with statistical significance(P<0.05). The dis-ease single rate of union group was 70.00%,which was significantly higher than that of single group (33.33%). PFS of union group was(11.23±2.17)months,which was significantly longer that of single group [(9.36±0.84)months],with statistical signif-icance(P<0.05). The incidence of leucopenia,alanine aminotransferase elevation,diarrhea,stomatitis and hand-foot syndrome in union group was significantly lower than in single group,with statistical significance(P<0.05). CONCLUSIONS:Xiaoaiping in-jection combined with irinotecan chemotherapy for advanced gastric cancer has obvious effect with a low incidence of ADR.

Objective To discuss the application value of the Nickel-Titanium patella concentrator(NT-PC) in surgical treatment of patellar fracture.Methods A retrospective analysis was made on clinical data of 32 cases of patellar fracture treated with the NT-PC in this hospital from January 2001 to December 2003.Results Re-examination of radiography at 12 weeks after surgery showed bone union was achieved in all the cases,with a smooth patellofemoral articular surface.The patella was preserved completely in all the comminuted fracture cases.According to the Lu's criteria,excellent and good outcomes were achieved in 26 and 6 cases,respectively,the rate of excellent or good results being 100%.The NT-PC was removed at 4 months ~ 2 years(mean,14 months) postoperatively without complications.Conclusions Application of NT-PC in the treatment of patellar fracture can preserve the comminuted patella completely,facilitate the reduction and fixation of the patellofemoral articular surface,and make early postoperative motions available.It is worthy of being recommended.

In recent years, there has been a growing emphasis on use of human adipose-derived stem cells (hADSCs) for cartilage tissue engineering owing to their ability to differentiate into chondrocytes, which is mainly induced by growth factors (GFs). In general, GFs for chondrogenic induction come from the transforming growth factor beta (TGF-beta) superfamily. To date, the most commonly used GFs for chondrogenes is TGF-beta1/3. However, the response of hADSCs to GFs may differ significantly from that of human bone marrow stem cells (hBMSCs). It has been reported that bone morphogenetic protein-6 (BMP-6) treatment induced TGF-beta receptor-I expression of hADSCs. It seems that these two cell populations varied strongly in their potency to undergo chondrogenesis in the same medium conditions. Here, we provide a concise review on various GFs used in chondrogenic differentiation of hADSCs in vitro.
Adipocytes ; cytology ; Cartilage ; Cell Differentiation ; Chondrocytes ; cytology ; Chondrogenesis ; Humans ; Stem Cells ; cytology ; TGF-beta Superfamily Proteins ; Tissue Engineering

Objective:To explore the effect of Shenfu Injection (SFI) on expression of Heme oxygenase-1 (HO-1) and iNOS (inducible nitric oxide synthase) in renal failure rats induced by intestinal ischemia-reperfusion (IR) and its possible mechanism. Methods:The model of intestinal ischemia-reperfusion was induced by clamping superior mesenteric artery for one hour and then releasing the arterial clamp for six hours. Wistar rats were randomized into three groups:IR+normal saline group,IR+SFI group and control group (C group). The serum creatinine and blood urea nitrogen were observed respectively. Expression of HO-1 and iNOS in rat kidney tissue was detected by immunohistochemitry and morphometry computer image analysis. The histological change of kidney was observed under light microscope. Results:①Compared with C group,expression of HO-1 and iNOS increased markedly in IR+ normal saline group (P

Objective To detect the expression of HSPA2 mRNA and protein in pancreatic cancer and corresponding adjacent nontumorous tissues, and investigate the expression of HSPA2 protein in pancreatic cancer and its association with clinicopathological characteristics.Methods Western blot and qPCR (real-time quantitative PCR) was used to evaluate HSPA2 expression in pancreatic cancer and corresponding adjacent nontumorous tissues.Immunohistochemistry was used to investigate the HSPA2 expression in pancreatic cancer and its association with clinicopathological characteristics.Results Both the mRNA and protein levels of HSPA2 were significant higher in pancreatic cancer tissues than those in their paired adjacent nontumorous tissues.High HSPA2 expression was detected in 68.8% (55/80) of pancreatic cancer tissues.HSPA2 protein expression was significantly associated with tumor differentiation, vascular invasion, and status of metastasis (P=0.011,0.005,0.012).Conclusion HSPA2 expression is elevated in pancreatic cancer, and positively correlated the process of invasion, metastasis and progression of pancreatic cancer.

The teaching and management of our eight-year program students is relatively new,so we should gradually study and explore the adapted cultivation pattern,learn and accumulate the successful experiences of the high starting point teaching in order to cultivate the qualified medical talents.

Objective: To compare the effects of three fluoride-containing agents on the remineralization of deciduous teeth in vitro. Methods: 36 deciduous teeth were randomly divided into four groups with 9 in each. The teeth were fenestrated on the labial middle surface and the rest parts of the teeth were covered with red nail polish.Every exposed area was etched,rinsed and puffed.Then the exposed areas were treated with 100 g/L(NH 4) 2MoO 2F 4(group ①) , 380 g/L Ag(NH 3) 2F (group ②) and APF-LaCl 3(group ③) for 3 minutes respectively, those in group④ were treated with deionized H 2O as the controls.Then,the teeth were placed into a bottle containing 5 ml remineralization solutions. 4 days later, the concentration of Ca 2+ in the remineralization solutions was measured. Results: The Ca 2+ concentrations (mmol/L) in the remineralization solution of group ①,②,③ and ④ were 0.338 9?0.116 5,0.570 0?0.145 5,0.517 8?0.084 8 and 0.120 4?0.046 8 respectively (P0.05:②vs③). Conclusion: The results indicate that all of the three fluoride-containing agents can promote the remineralization of deciduous teeth in vitro, and that both APF-LaCl 3 and 380 g/L Ag(NH 3) 2F are more effective than 100 g/L(NH 4) 2, MoO 2 F 4.

The angiographic findings of 18 patients with vena cava syndrome were reported.The venacavographies showed the extent,degree of stenosis or obstruction,and collateral vascular dilatation.The vena cava displaced and stenosis suggested that there was space occupying lesion in the adjacency.The abdominal aortography could show streak like vessels supplying the tumor in the periphery of vena cava.The casuse,clinical feature and angiographic classification were briefly discussed.The angiography is considered an accurate method of diagnosing vena cava syndrome.

BACKGROUND:In the early development of zebrafish embryos, cels divide and proliferate rapidly, but low concentration of deguelin can delay the development of zebrafish embryos.
OBJECTIVE:To observe the effect of different concentrations of deguelin on cyclin D1 gene expression in zebrafish embryos.
METHODS:Though normal fertilization, zebrafish embryos that incubated to the 2-cel stage (about 0.75 hour after fertilization) and shield stage (6 hours after fertilization) were colected and put into 12-wel plates treated with 100, 200, 400 nmol/L deguelin at 28.5℃in an incubator til the shield period and 24 hours after fertilization, respectively. Simultaneously embroys treated with 1% dimethyl sulfoxide solution were as a control group, cultured in the same conditions. Cyclin D1 RNA probes were prepared for the whole mountin situhybridization, observing staining by an upright fluorescent microscope camera to detect the effect of deguelin on cyclin D1 expression in zebrafish embryos.
RESULTS AND CONCLUSION:Deguelin showed significant negative regulation on the expression of cyclin D1 gene in zebrafish embryos. Cyclin D1 expressed in outsourcing cels in embryos of shield stage, and a significant reduction in the expression of cyclin D1 came up with the increasing concentrations of deguelin. In the 400 nmol/L deguelin treatment group, there was nearly no expression of cyclin D1. Cyclin D1 expressed in the brain, central nervous system, immature eye, somites, trunk, and tail of embryos at 24 hours after fertilization, and reduced significantly in the 100 nmol/L deguelin treatment group, especialy in the proliferative area. In the 200 and 400 nmol/L treatment groups, the embryonic development slowed down signficantly, and cyclin D1 gene mainly expressed in the dorsal ectoderm cels.

This study was aimed to investigate the effect of Rheum tanguticum on expression of serum endothelial nitric oxide synthase (eNOS), endothelin (ET) and 6-keto-prostaglandin F1α (6-Keto-PGF1α) in uric acid nephropathy rat model. After 3 weeks of treatment with different extractions of Rheum tanguticum on uric acid nephropathy rat model, levels of serum BUN, Cr and UA were measured by biochemical analyzer. ELISA method was used to measure the expression of serum eNOS, ET and 6-Keto-PGF1α. Meanwhile, pathological changes of renal tissues were observed by light microscope. The results showed that compared with the normal control group, the levels of serum BUN, Cr, UA and renal index were higher in the model group (P< 0.05). Compared with the model group, the concentration of eNOS in the model group was obviously higher in the rhubarb ethanol extraction group, rhubarb water extraction group, and allopurinol group (P< 0.05). Concentration of ET was obviously reduced in the rhubarb water extraction group and allopurinol group (P< 0.05). There was no statistical difference with the rhubarb ethanol extraction group. There was no significant difference in the concentration of 6-Keto-PGF1α among groups. Lots of inflammatory cells and uratic crystallization were observed by light microscope in renal tissues among rats in the model group. Damages of renal tissues in medication groups were alleviated than the model group. It was concluded that Rheum tanguticumcan alleviate the damage of uratic crystallization and inflammatory cell infiltration, increase the concentration of serum eNOS, and decrease the concentration of serum ET. Thus, it can reduce the damage of renal endothelial cells and protect renal functions. The effect of rhubarb ethanol extraction on reducing ET expression was less than the water extraction.