ObjectiveTo explore the mechanisms of proliferation and apoptosis of K562 cells induced by cytarabine. MethodsDifferent doses of cytarabine on the proliferotion at K562 cell 24h,48h,72h were detected by MTT. Cell cycle And Apoptosis rate were detected by FCM. ResultsEach concentration of cytarabine obvious inhibited the growth of K562 cells. In the same concentrations,function of proliferation inhibition with cytarabine increased by the extension of time,which showed dose and time effect relations. Cytarabine on K562 cell,G0/G1 phase cell proportion of role as cytarabine increased when extension of time, S period and the G2/M period cells were decreased.Apoptosis of K562 cell in proportion as drug concentration and effect time increases, which present Cytarabine timeconcentration dependence. CondusionCytarabine was apparent inhibition to proliferation of K562 cell leukemia cell, which could promote K562 cell apoptosis by time and concentration dependence way.

Type 2 diabetes is associated with a marked increased risk of cardiovascular disease (CVD).Treatment for dyslipidemia in diabetes reduces cardiovascular event.This article reviewed the clinical trials of lipid lowering in diabetic subjects,treatment goals for lipoprotein therapy,and how to choose lipid lowering agents.

0.05) and associated with histopathologic grade and clinical stages (P

Objective:To explore clinical significance of the further method dealt with in ASCUS(atypical squamous cells undetermined significance) of cervical smear.Methods:118 patients with ASCUS were detected by colposcopy and high risk HPV test,simultaneously make biopsy of cervical guided by colposcopy.Result:By diagnosed with pathology,chronic cervical inflammation is 72 cases,46 cases were CIN(cervical intraepithelial neoplasia).In this study 40 cases were positive in HPV test which 33 cases are CIN(?2=48.18,P0.05).Conclusion:There are high CIN csses in Women with ASCUS whose should be paid attention to and strengthen management.Colposcopy and high risk HPV test are efficiency method to ASCUS,especially high risk HPV test is positive that need detective by colposcopy.The combination of colposcopy and high risk HPV test can improve detective rate and accuracy of cervical intraepithelial neoplasia(CIN).

OBJECTIVE: To summarize research progress of tissue-engineered tendon stent material and its application for repairing tendon defect. METHODS: A computer-based online search was conducted with the key words of "tissue engineering, tendon, scaffold" in both Chinese and English from January 1993 to October 2009. Articles about dynamical performance of tissue-engineered tendon,experimental research and clinical application of tissue-engineered tendon were included, but duplicated studies and Meta analysis were excluded. Among 38 articles, 4 major viewpoints were discussed: research progress of tendon tissue engineering; biodynamical analysis of tissue-engineered tendon stent materials; application of biomaterials for tendon tissue engineering; clinical application of tissue engineering for repairing tendon defect. RESULTS: Natural polymer and synthetic macromolecule were major materials in tendon tissue engineering. The preparation and selection of stent materials depended on producing imitated materials with advanced performance and structure based on artificial synthetic materials, preparing natural derivative stent materials based on natural extracellular matrix, and constructing tissue-engineered tendon based on original bio-structure and bio-performance of biological tissue. Construction of tissue-engineered tendon must simulate three-dimensional physical environment in vivo. Microcomputer-controlled step motor was used to force tendon-matrix compound stent in order to increase proliferation velocity of tendon cells and secretory volume of collagen and develop into stress direction. CONCLUSION: Physical stimulation is simple, thus technique and instrument for constructing artificial tendon need to be further studied. Additionally, living implants which may completely replace body tissue and organ function still need to be further studied and solved.

BACKGROUND:Tumor stem cels have self-renewal, drug resistance and metastasis tumorigenicity, which play an important role in occurrence, development and metastasis of tumors. Currently, there are two methods to identify tumor stem cels, namely, in vitro tumor sphere culture experiments and in vivo mouse tumorigenic experiments. However, there ia a lack of reports regarding clinicaly enriched gastric cancer cels by chemotherapy. OBJECTIVE:To investigate the enrichment of rat gastric cancer stem cels by cisplatin, and to explore the screening methods for their surface marker proteins. METHODS: BCG-823 gastric cancer model was established in rats, and then rat models were randomized into two groups: rats in experimental groups were subjected to intravenous injection of 0.1, 0.2, 0.25, 0.3 g/L cisplatin via the tail vein; those in control group were injected with normal salinevia the tail vein. After three courses of chemotherapy, gastric stem cels-enriched tissues were colected. Tumor surface proteins were extracted using high-throughput protein microarray and identified by western blot assay. Effects of cisplatin on enrichment of rat gastric cancer stem cels and screening methods for surface marker proteins were compared. RESULTS AND CONCLUSION:Cisplatin at a dose of 0.3 g/L×200μL exhibited the best therapeutic effects, and moreover, with the dose increasing, the tolerance became worse and the incidence of adverse reaction became higher. Transplantation tumors were verified by hematoxylin-eosin staining. Western blot test results were similar to the findings of protein microarray method, that is, HLA-DQ, PMP22 and Claudin7 protein expressions increased in gastric tissues, but HLA-DR, CD14, CD16 and CD56 protein expression decreased. These findings suggest that cisplatin can be used to enrich gastric cancer stem cels in rats, and to successfuly screen the corresponding surface marker proteins.

With the development of funcgonal laboratory teaching,the advantages of integrating laboratory resources have been embodied in someway,improving the students'experiment level and their comprehensive analysis ability.But many new problems have also shown up such as unreasonable curriculum,construction of teaching staff.By reinstalling experiment courses,integrating the experiment teaching staff,and exploring the solution to the existing problems,the teaching quality of functional experiment can be promoted

Objective To investigate the clinical effect and safety of laparoscopy on ovarian benign tumor. Methods The data of 68 patients, underwent laparoscopic operations on ovarian benign tumor from Jan 2009 to Jan 2010, were analyzed retrospectively. Sixty eight patients underwent laparotomy were randomly enrolled as control. The operative time, the blood loss, the average admission day and the average postoperative exhaust time were compared between two groups. Results We found no significant differences in the comparation of operative time ( [ 63.60 ± 7.71 ] min vs. [ 62. 40 ± 8. 90 ] min, t = 0. 663, P ＞ 0. 05 ), while the blood loss, the average postoperative exhaust time, the average admission day of the treatment group were significantly lower in the objectives than control ( [ 68.00 ± 14. 90 ] ml vs. [ 119.00 ± 19. 60 ] ml, [ 17.30 ±2.40]h vs. [34.60 ±2.80] h, [3.90 ± 1.40] days vs. [7.70 ± 1.50] days,t =2.664, 2. 523,2.724,Ps ＜0. 05). Conclusion Laparoscopy is the first choice of method in treating ovarian benign tumor given its minimal invasion and rapid recovery.

Objective The aim of this study was to examine hope level in patients with coronary heart disease after per-cutaneous coronary intervention and explore correlation with illness perception.Methods 176 patients in outpatient department were recruited after per-cutaneous coronary intervention.The Herth Hope Index (HHI),Illness perception Questionnaire-Revised and demographic and clinical characteristics were collected.Results The hope level score of patients after per-cutaneous coronary intervention was (36.18±3.21),the hope level was correlated with seven dimensions of illness perception and was influenced by degree of education,income level,whether the disease is acute or chronic and control belief.Conclusions The patients after per-cutaneous coronary intervention show high level of hope and is affected by many factors,this provides the basis for taking effective measures to improve the patients' level of hope.

Objective To study the effect of 3％ hypertonic saline(HS) and 20％ mannitol on cerebral oxygen metabolism in patients undergoing neurosurgery for supratentorial glioma.Methods 56 patients undergoing neurosurgery for supratentorial glioma were evenly divided into two groups.Group A(n =28) was treated by 3％ hypertonic saline(5.35ml/kg).Group B (n =28) was treated by 20％ mannitol(1 g/kg).CSFP was tested before hypertonic saline or mannitol infusion (T0),immediately (T1),15,30,60,120min after infusion (T2 ～5).Various blood-gas indexes were observed at To,T3 ～5.CaO2,CjvO2,Da-jvO2 and CERO2 were calculated.Results CSFP significantly dropped from T2 to T5 in both groups (P ＜ 0.05).Da-jvO2 and CERO2 were significantly reduced at T4～5 in both groups (P ＜ 0.05).There was no significant difference between the two groups.Conclusion 3 ％ HS is as efficient as 20％ mannitol in reducing intracranial pressure and improving cerebral oxygen metabolism in patients undergoing neurosurgery for supratentorial glioma.