Objective To compare the effects of vasopressin and adrenaline on cerebral resuscitation in rats. Method Sixty male SD rats were used to set the animal model of cardiac arrest and were randomly (random number) divided into 4 groups (n = 15/group): sham operation group (sham-gro), negative control group (neg-gro), vasopressin group (vas-gro) and adrenaline group (adr-gro). Blood pressure was recorded, and brain tissue samples were obtained. Results There was no significant difference in the recovery rate between vas-gro and adr-gro 30 min after resuscitation (P ＜0.05). The mean blood pressure in vasgro was higher than that in adr-gro within 30 min (1,2, 5, 10, 20, 30 min) after restoration of spontaneous circulation. The measured MDA, SOD and GSH were significantly different (P ＜ 0.05) between each two groups among vas-gro, sham-gro and adr-gro. Conclusions The recovery rate was similar between vasopressin group and adrenaline group during cardiopulmonary resuscitation in rats. However, vasopressin can to maintain the mean arterial pressure at a higher level after resuscitation, which can the increase the cerebral perfusion and reduce brain cell damage.

OBJECTIVE:To investigate the relationship between the change of RAAS activity and plasma glucose after one year therapy of hydrochlorothiazide. METHODS:A total of 119 hypertensive patients were enrolled in this study. The activity of RAAS and plasma glucose level were measured at baseline. Patients were given 12.5 mg hydrochlorothiazide once a day for one year. Then the relationship between activity change of RAAS and the level of plasma glucose were analyzed. RESULTS:After one year treatment,the decrease of plasma glucose in PRA elevated group and PRA unelevated group were(-0.26?0.26)mmol?L-1 and(-1.36?0.23)mmol?L-1respectively,and there was statistical significance in difference(P

Aim To investigate the pharmacokinetics of nodakenin(ND),one of main chemical constituents in the rhizome and root of Notopterygium incisum Ting ex H.T.Chang and N.forbesii Boiss.,in rats.Methods SD rats were used throughout the experiment and their body weights ranged from 200 to 220 g.All of them were distributed randomly to different test groups(5 rats per group).Nodakenin was administered at a single dose of 80 mg?kg-1 via tail vein.The blood was collected from the orbital venous plexus at different time points.A reversed-phase high performance liquid chromatography(RP-HPLC) method,in which the analyte was isocratically eluted and the external standard method was used,was developed to determine concentrations of ND in rats.The main parameters of pharmacokinetics were calculated by 3P87 software.Results Under the established conditions of HPLC,the retention time of ND was 6.7 min.The calibration curve was Y=2.0?10-4 X+0.4(r=0.9999) with a validated quantitation range from 0.2 mg?L-1 to 80 mg?L-1.The lower limits of detection(LLOD) and quantification(LLOQ) in rats plasma were 0.01 mg?L-1(S/N=3) and 0.1 mg?L-1(S/N=10),respectively.At the concentrations of 1.0,10.0 and 40.0 mg?L-1,extraction recoveries were ranged from 76.23% to 83.72%.The RSD of the precision intra-and inter-day were less than 5.60%(n=3).ND was stable at room temperature and freeze-thaw cycles.The concentration-time course of ND was best fitted to a two-compartment open model after a single intravenous injection dose of 80 mg?kg-1,which the main pharmacokinetic parameters t1/2?,t1/2?,AUC,Vc,Vp,Vss,CL were 7.02 min,219.27 min,1384.34 mg?min?L-1,0.92 L?kg-1,6.04 L?kg-1,6.96 L?kg-1,0.06 L?kg-1?min-1,respectively.Conclusion The developed method is shown to be rapid,accurate and simple,and can satisfy the requirement of pharmacokinetic study of ND in rat.ND was distributed widely and eliminated moderately after a single intravenous injection dose of 80 mg?kg-1 in rats.

Esophageal cancer is one of the tumors with high morbidity rates in China, and surgery remains to be the preferred treatment. However, certain complications such as anastomotic fistula, lung infection, respiratory failure, and circulatory failure occur after surgery. Postoperative enteral nutritional support could not only maintain and improve the nutritional status of the body but also re-duce complications and maintain normal immune function. This review focuses on the progress in postoperative enteral nutrition in pa-tients with esophageal cancer.

In most non-excitable cells,the store-operated calcium channel (SOCC) in the plasma membrane is the major calcium entry pathway.SOCC plays an important role in calcium signals that control many cancer cellular processes such as adhesion,secretion,movement,gene expression,proliferation,division and apoptosis.Stromal interaction molecule 1 (STIM1) and Orail are the two important parts of SOCC.So overexpression or suppression expression of STIM1/Orail can regulate SOCC activity and biological characteristics of cancer cells.However,SOCC plays a contradictory role in cancer cells,which can both promote tumor progression and inhibit tumor progression.SOCC is expected to become a new target for the treatment of tumors,but its complicated mechanism needs to consider both types of tumor cells and the external stimulators of tumor cells.

Objective To explore effects and mechanism of penhyclidine hydrochloride (PHC) on myocardial ischemia reperfusion injury in rat.Methods Sixty Sprague Dawley (SD) adult male rats were randomly divided into sham operation group(SHAM group),myocardial ischemia reperfusion injury group(MIRI group) and penhyclidine hydrochloride treatment (PHC group),and 20 rats in every group.Compared left ventricular function of 3 groups after 24 h of ischemia reperfusion.Selected 10 rats of every group and compared the myocardial infarction size after ischemia reperfusion,and the less 10 rats' the level of tumor necrosis factor-α (TNF-αt) and nuclear factor-kB(NF-kB) in myocardial tissue were measured.Results After 24 h of ischemia reperfusion,there was no significant myocardial infarction size in SHAM group,while that in MIRI group were (39.4± 3.7)％,higher than that in PHC group (20.5 ± 2.3)％,and the difference was significant (P ＜ 0.01).The level of left ventricular end-systolic volume (LVESV) of rats in SHAM,MIRI and PHC groups were (0.138 ± 0.024),(0.457 ± 0.032) and (0.280 ± 0.027) ml respectively,left ventricular end-diastolic volume(LVEDV) were (1.012 ± 0.065),(1.395 ± 0.076) and (1.183 ± 0.102) ml respectively,and left ventricular ejection fraction (LVEF) were (85.6 ± 7.3) ％,(43.2 ± 4.5) ％ and (64.8 ± 5.1) ％ respectively,and there were statistically significant differences between groups (F =54.12,32.70,35.46,P ＜ 0.001).The level of TNF-αtand NF-kB in rats of SHAM,MIRI and PHC group were (5.4 s0.7),(34.2 ±2.9) and (21.6 ± 1.3) pg/mg respectively and (87.6 ± 7.3),(134.2 ± 9.6) and (116.3 ± 8.7) respectively,and there were statistically significant differences between groups (F =59.40,17.97,P ＜ 0.001).There were statistically significant differences between MIRI and SHAM groups,PHC and SHAM groups in terms of above factors (P ＜0.05).Conclusion PHC can decrease the inflammatory reaction and protect the myocardial ischemia reperfusion injury in rat.

The objective of this study is to evaluate the relative bioavailabilities (RBA) of iron in Soybean meal and egg yolk powder by means of the rat hemoglobin repletion method, and to analyse their iron contents by the atomic absorption spectrometric determination.The iron content of soybean meal is 159.78 ppm, RBA is 67.24%; the iron content of egg yolk powder is 128.65ppm, RBA is 70.90%. This means that both soybean and egg yolk are of high iron contents, and their RBAs are not too inferior, indicating that the absolute amounts of available iron are still high. So both of them should be considered a rather good source of dietary iron.Using the total iron consumption and the final total hemoglobin of each animal during the hemoglobin repletion period as a dose-response index to calculate the RBA is thought to be reasonable.

BACKGROUND:Scholars are stil looking for ideal bone tissue-engineered scaffolds, and three-dimensional (3D) printing technology is a novel construction method. In the meanwhile, bone extracel ular matrix is becoming a hotspot in osteogenic induction. OBJECTIVE:To construct the polycaprolactone/bone extracel ular matrix scaffold using 3D printing technology and co-culture method, and to detect its osteogenic property. METHODS:216 3D-printed polycaprolactone scaffolds were divided into group A (96 pores, n=72) and group B(48 pores, n=144). Passage 5 bone marrow mesenchymal stem cel s from Sprague-Dawley rats were seeded onto the two kinds of polycaprolactone scaffolds, and the group A was used for alizarin red staining and Masson staining, while the group B for col agen and glycosaminoglycan detection at 1, 2 and 3 weeks of incubation. Afterwards, the scaffolds at 1, 2 and 3 weeks of culture were decel ularized and labeled as groups AE1, AE2, AE3, BE1, BE2 and BE3. Then passage 5 bone marrow mesenchymal stem cel s from Sprague-Dawley rats were seeded onto each scaffold again, and the former three groups underwent alizarin red staining, and the latter three were used for calcium, alkaline phosphatase activity and DNA quantitative analysis at 1, 2 and 3 weeks of culture. RESULTS AND CONCLUSION:Masson staining, glycosaminoglycan and hydroxyproline quantitative analysis showed that the extracel ular matrix on the composite scaffold increased with time. Alkaline phosphatase activity revealed that the composite scaffold had a significantly stronger osteogenic differentiation than the normal polycaprolactone scaffold (P<0.05). Alizarin red staining and calcium quantitative analysis showed that the mineralization of the composite scaffold was more obvious than that of the normal polycaprolactone scaffold (P<0.05), but the total DNA analysis did not differ significantly between scaffolds. These results suggest that the composite scaffold with extracel ular matrix is constructed successful y using the 3D technology and co-culture method and exhibits a better osteoinductivity.

Telemedicine is a combination of modern computer technology, communication technology and medical technology. It has a promising application potential. This paper mainly overviews the history of telemedicine, especially the development of wireless telemedicine. With the classification and comparison, the opinion on the future of wireless telemedicine is given according to the author's research work. Portable telemedicine system is the trend of telemedicine.

Traditional open esophagectomy is associated with significant mortality and morbidity.Some researches have suggested that the incidence of the complications may be decreased with minimally invasive esophagectomy which has equivalent survival rate compared with open esophagectomy.It may become a tendency of esophageal surgery.This article reviewed the progress of minimally invasive esophagectomy.