ObjectiveTo investigate the relationship between carotid atherosclerosis and progressing stroke.Methods103 patients with acute cerebral infarction within the first 24 hours from onset were tested with carotid color doppler ultrasonography during the first week from admission. Univariate and multiple logistic regression analyses were performed to examine the relationship between carotid intima media thickness (IMT), plaque structure, the degree of artery stenosis and the early deterioration of cerebral infarction.ResultsPrevalence of carotid plaque and thickness of IMT in patients with progressing stroke were higher than that in non progressing patients. In logistic regression, presence of carotid plaque was independent predictors of progressing stroke. Plaque size, extent of artery stenosis and IMT were not included in the regression model.ConclusionPlaque structure and its chemical feature may be the cause of progressing stroke.

AIM:To explore the role of α7 nicotinic acetylcholine receptor (α7nAChR) in anti-inflammation of glucocorticoids (GCs) at physiological concentrations .METHODS: MTT assay was used to measure the viability of BV-2 cells, which were processed by hydrocortisone at different concentrations .On the basis of inflammatory model induced by LPS in BV-2 cells, experimental groups were divided as follows:(1) control;(2) LPS;(3) GCs+LPS;(4) methyl-lycaconitine ( MLA)+GCs+LPS.The levels of TNF-αand IL-1βin the cell supernatants were detected by ELISA .RE-SULTS:Hydrocortisone at concentrations of 2 000 and 1 000 nmol/L decreased the cell viability to (76.9 ±5.5)% and (90.8 ±7.3)%, respectively, indicating the cellular injury by GCs at over-physiological doses.LPS significantly induced the releases of TNF-αand IL-1βin a time-and dose-dependent manner in BV-2 cells.Hydrocortisone at physiological con-centrations (500 and 250 nmol/L) reduced the releases of TNF-αand IL-1βin BV-2 cells stimulated by LPS, and MLA at concentration of 10 nmol/L antagonized the anti-inflammatory effect of GCs .CONCLUSION:α7nAChR is involved in the anti-inflammatory effect of the physiological concentrations of GCs .

OBJECTIVE:To observe the synergism and attenuation of Fermented Radix Astragali Powder(FAP)on Cyc-lophosphamide(Cy),a tumor chemotherapeutics,and to explore more economical and effective assistant chemotherapeutics.METHODS:In synergism experiments,the mice tumor models(S180 and H22 solid tumor and H22 ascitic tumor)were used to observe the effects of FAP combined with Cy on tumor control rate and survival time of mice.In attenuation experiments,the effects of FAP against toxic reactions including myelosuppression,atrophy of immune organs,and lesions on renal function induced by high dose of Cy in mice were observed.RESULTS:FAP did enhanced the the antitumor effect of Cy on S180 and H22 solid tumor,lessened Cy-induced toxic reactions such as aleucocytosis,the reduction of the nucleated cells in bone marrow and the weight loss of spleen in mice,meanwhile,it showed protective effect on renal function yet without no significant influence on hepatic function.CONCLUSION:FAP has synergistic and attenuation effects on Cy.

Proteome is defined as all proteins expressed in a cell, a tissue or an organism. Proteomics, a science of understanding the life activity law at the whole protein level, takes proteome as a research object; it is also a scientific method for quantitative detection of one or more protein populations or proteomes in a cell or tissue. Cerebral ischemic preconditioning has been one of the research hotspots in the field of neuroscience for decades. Its neuroprotec-five mechanism is associated with the changes of protein expression levels after cerebral ischemia, Therefore, proteomics has become more and more important in its mechanism study. This article mainly presents the major techniques of proteomics and their application in the studies of cerebral ischemic preconditioning.

Objective To investigate the relevant factors of hyperthyroidism complicating hyperthyroid heart disease.Method Among 48 cases,those factors including age,course of disease,condition and regular treatment were analyzed with Logistic multiple factor reg-ression analysis.Result The hyperthyroid heart disases was related with age,course of disease,condition and whether to treat regularly.Conclusions There are many factors to lead to hyperthroidism complicating hyperthyroid heart disease.It should be paid attention to in those patients with elder,longer course of disease,serious condition and no regular treatment.

Based on the necessity of carrying out bioethics education to the probationer nurses, this paper put forward to introduce psychological sitcom to the probationer nurses′ bioethics education and described the practice process in detail. It also discussed the mechanism to improve the effectiveness of bioethics education from the per-spectives of creation, dramatization, and performance of psychological sitcom.

The suprachoroidal space (SCS) is the potential space between the sclera and choroid.Drugs delivered through SCS can bypass the sclera,avoiding clearance by conjunctival and scleral blood vessels and lymphatic circulation,so that more drugs can reach the disease tissues such as choroid and retina.SCS drug delivery does not disrupt the ocular integrity,is safer than the intravitreal drug injection and more effective than trans-scleral drug delivery.In addition,SCS delivery only needs a very small volume of drug,which makes it possible to be carried out in multiple parts of the sclera,and the specific disease area can be more precisely targeted.SCS drug delivery is suitable for the treatment of choroidal and retinal diseases.However,currently SCS drug delivery is still a novel field and many aspects need to be more in-depth studied,including its safety,delivery methods,drug formulation and effectiveness.

Objective To explore the clinical characteristics and diagnosis of primary small intestinal tumor(PSIT). Methods Retrospective analysis of the clinical and pathological data of the 31 PSIT cases was made. Results Duodenum was the most common site 67.74% for PSIT. The confirmed diagnosis rate was 54.84%(17/31)(duodenum was 66.67%,14/21; ileum and jejunum was 30%, 3/10, respectively ). 3 cases were benign tumors and 28 were malignant tumors.The adenocarcinoma was the most frequently seen malignant PSIT followed by lymphoma and often in duodenal. The features of the small intestinal tumor could be abdominal pain,abdominal mass,hemorrhage,obstruction,jaundice and fever.The chief method for diagnose was barium meal X-ray examination, especially with the hypotonic contrast X-ray examination and endoscopy.Superior mesenteric arteriography sometimes helped in determing site of gastrointestinal haemorrhage. CT scan could be helpful in diagnosis. Conclusion Duodenal adenocarcinoma is most common PSIT,followed by malignant lymphoma.Endoscopy is the best way to find out the tumor located in duodenum and hypotonic contrast X-ray examination is effective in diagnosis and locating of small bowl tumors.Superior mesenteric arteriography is a good way to find out angioma and leiomyoma. CT scanning are also helpful.

Aim To observe the effect of metformin(MF)on inhibinB,activinA and follistatin in rat of polycystic ovary(PCO),analyzing the possible therapeutic mechanism and therapeutical effect from ovaries local cytokine.Methods The PCO rat models were set by insulin combined with human chorionic gonadotrophin(HCG).The levels of serum gonadal hormone and insulin(INS)were detected by radioimmunoassay.Using the sandwich ELISA technique the expressed level of serum inhibinB(INHB),activinA(ACTA)and follistatin(FS)was detected.Results Metformin could decrease the level of testosterone(T),luteotropic hormone(LH)and INS in rat serum,but it increased the level of ACTA in rat serum and the rate of ACTA/INHB.There was negative correlation between INS and ACTA,and positive correlation between INS and INHB.Conclusion MF can regulate the expression of ovaries local cytokines such as INHB,ACTA and FS.