OBJECTIVETo investigate necessity of the further processing for adsorptive resin material.

METHODSAccording to GB/ T 16886.4, coagulation tests, haematology tests, in vitro spontaneous platelet-adhesion test and complement activation tests were conducted respectively to assess the in vitro blood compatibility of adsorptive resin material.

RESULTSIn comparison with polypropylene, he adsorptive resin material appears to be a very slight activator to the endogenous blood coagulation system, it can inhibited the exogenous blood coagulation system very significantly (P < 0.01), it can adhere to fibrinogen, red blood cells, reticulocytes very significantly (P < 0.01), and platelet significantly (P < 0.05). The adsorptive resin material is also revealed to be a very significant activator (P < 0.01) to the complement system.

CONCLUSIONSThe in vitro blood compatibility assessment witnesses that it is very necessary for the adsorptive resin material to go through the certain special processing(such as the surface modification and the microencapsulation technology, etc) before its application to human being.

Magnetic mediated particles can be located within tumor volume through special ways are can be heated while exposed to an externally alternating magnetic field,thus kill the tumor cells.Compared with othen hyperthermia a major advantage of magnetic mediated hyperthermia is that of targeting tumor specially and selectively.The evidences of clinical research demonstrate that magnetic mediated hyperthermia will have a promising future and play an important role in tumor therapy.

Rapid and accurate diagnosis of pathogens is the responsibility of the clinical microbiology lab.Effective implementation of this function requires standard procedure of specimen collection and processing.In order to improve the ability of infectious disease diagnosis,clinical microbiology laboratory should strengthen the communication with the clinicians,to improve the quality and quantity of specimens.Meanwhile,microbiology laboratory should learn some clinical knowledge and understand the demand and development of clinical practice.Clinical microbiology lab should emphasize patient-oriented and multidisciplinary close cooperation,for improvement of pathogen diagnosis level.

In this paper, some study methods of pharmacokinetic, the explanation ways of data and experimental methods of transdermal therapeutic system(TTS) were described. The advancement of modern science accelerates the development of pharmacokinetic of TTS and makes more progress in this field.

Objective To measure the sizes of the left and right internal jugular veins (IJV) and transverse sinuses (TS) with three dimensional contrast‐enhanced MRA(3D CE‐MRA) ,and discuss the correlation of cerebral venous sinus and IJV .Methods One hundred and ten patients without vasculopathy and blood flow abnormalities were selected for 3D CE‐MRA ,55 cases for each gender .The images were taken three‐dimensional reconstruction on work‐station by different software .The normal IJV and TS was measured and the statistical comparison between the bilateral IJV and TS was analyzed .Results (1)The inside diameter of IJV re‐duced in turn at the measurement point of J1 ,J2 and J3 .The internal diameter of the right side of IJV was broader than left one ,and there was significant difference between the size of left and right side (P<0 .05) in these measurements .The internal diameter of the right side of TS was broader than left one ,there was difference between the sizes of the left and right of TS (P<0 .05) .(2)The proportion of R>L ,R

The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 ?g S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure pathology was characterized by swelling and necrosis in the inner and outer segments of photorecepter cells. This result suggests that S antigen has significant effect on inducing experimental autoimmune uveitis and the outer layer of retina is the sensitive target organ of S antigen.

Objective To investigate expression and significance of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum and placental tissue of preeclampsia maternal.Methods 74 cases of preeclampsia pregnant women as research subjects,which 39 cases of severe preeclampsia,35 cases of mild preeclampsia,selected 30 cases of normal late maternal as the control group.Three groups of maternal serum levels of MMP-9 and of NGAL were detected by enzyme-linked immunosorbent assay.RT-PCR was used to test placenta MMP-9 mRNA,and NGAL mRNA level.Results Preeclampsia maternal serum levels of MMP-9 and NGAL level were significantly higher than those of severe preeclampsia pregnant women serum level of MMP-9 and NGAL were significantly higher than mild preeclampsia (t =3.127,2.846,3.508,3.284,all P ＜ 0.05).Preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than the control group (t =-2.842,-2.728,-3.532,-3.276,(a)ll P ＜ 0.05),severe preeclampsia placentas of MMP-9 and NGAL were significantly higher than mild preeclampsia(t =2.732,2.741,all P ＜ 0.05),severe preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than in patients with mild preeclampsia (t =-2.681,-2.587,all P ＜ 0.05).Preeclampsia maternal serum levels of MMP-9 and NGAL was positively correlated with placental tissue mRNA levels of MMP-9 and NGAL mRNA level was positively correlated (P ＜ 0.05).Conclusion Preeclampsia maternal serum MMP-9 and NGAL were significantly elevated,placental MMP-9 mRNA and NGAL mRNA levels were significantly decreased,suggested that they may be involved in the pathogenesis of preeclampsia.

Objective:To discuss the clinical effect of preoperative interventional chemotherapy and pathological changes in uterine cervical cancer.Methods:preoperative interventional chemotherapy by femoral intubatton were done in 21 patients with uterine cervical cancer.The project of chemotherapy was bleomycin 45mg and cisplatin or oxaliplatin 80mg/m2.Rusults:21 cases of uterine cervical cancer including 7 cases ofⅠstage and 14 cases of Ⅱstage received one or two times of preoperative interventional chemotherapy.All of the patients successfully completed cervical cancer radical therapy.All of the specimens were sent for pathological examination.A lot of lymphocyte cells infiltration and necrosis were found in the cancer tissues.Conclusion:preoperative interventional chemotherapy for uterine cervical cancer proves to reduce the local cancer size as well as decrease the bleeding of cancer.The patients can receive cervical cancer radical therapy after the interventional chemotherapy.

In this work, rare specific binders to botulinum neurotoxin antigen were selected from human phage immunized library through three rounds of panning in vitro. Of them, clone ScFvB17 is of high affinity and specificity to target antigen. B17 is of 750bp encoding 250 amino acid. Analysis results of gene structure showed V-genes of B17 belonging respectively to VH4 or? chainⅡ family are new antibody Fv genes. More important result showed that recombinant ScFvB17 expressed in E.coli could bind specific for neurotoxin competing with antiserum. Successful selection of human phage antibody library and obtaining specific ScFv against botulinum neurotoxin serotype A provide useful materials for further study on detection of neurotoxin and human engineering therapeutic antitoxin.