Objective To establish a HPLC method for determining and comparing the contents of diosbulbin B in
Dioscorea bulbifera L. from different regions. Methods The medicine powders were extracted twice by water, then the water extracts were combined and detected by HPLC at 35℃,with Kromasil-C18 column (4. 6 mm×250 mm,5μm) and a mobile phase of acetonitrile-water-glacial-acetic acid (34660. 1). The flow rate was 1. 0 mL·min-1 and the detection wavelength was 210 nm. Results The linear range of diosbulbin B was 26. 0-260. 0 μg·mL-1(r=0. 999 9). The average content of diosbulbin B in Dioscorea bulbifera L. from different areas was that of Hebei>Guangxi>Jiangsu>Sichuan>Zhejiang. Conclusion The method is simple,quick,accurate and suitable for the determination of diosbulbin B in Dioscorea bulbifera L. from different regions.

Objective To investigate the effects of Xinnaoxuemianning combined with statins on carotid atherosclerotic plaque in patients with cerebral infarction. Methods 180 cerebral infarction patients were divided into the observation group (90cases) and the control group (90cases) by random number table method. The control group was given atorvastatin calcium tablets and the observation group was given additionally Xinnaoxuemianning. The changes and prognosis of carotid atherosclerotic plaques were evaluated before and after treatment, and the incidence of adverse reactions was counted. Results There were no differences in the carotid atherosclerotic plaque, TCM syndrome score and neurological deficit severity between the two groups before treatment. After treatment, the above indexes were significantly improved, and the increasing degree of the observation group was better than that of the control group. The incidence rate of adverse reactions was lower in the observation group than that in the control group (5.56％ vs.13.33％) (P<0.05). Conclusion Xinnaoxuemianning can reduce the carotid atherosclerotic plaque area and correct the unstable plaques in patients with cerebral infarction.

Animals ; Antineoplastic Agents ; pharmacology ; therapeutic use ; Brain Neoplasms ; drug therapy ; secondary ; Breast Neoplasms ; drug therapy ; metabolism ; pathology ; Cell Proliferation ; drug effects ; Humans ; Quinazolines ; pharmacology ; therapeutic use ; Receptor, Epidermal Growth Factor ; metabolism ; Receptor, ErbB-2 ; metabolism

Objective IL-2,6 production by PBMNC (peripheral blood mononucleat cells) were ineaeured with an attempt to illustrate the immune function of children with bronchopneumonia.Methods The biological activities of IL-2,6 were measured by CTLL and CESS cell lines respectively In addition, mitogen-stimulated blastogenic transformation (MSBT) was also measured. The results were analysed statistically. Patients or Other Farticipants T enty-eight cases of children with bronshopneumonia, male, 16,female: 12. Age: 8 months to 7 years, average 3. 9 years. 15 normol controls, male: 8, female 7.Age: 2 to 6 years, average 3 .5 years.Interventions 5～6ml of venous blood was obtaincd, followed PBMNC were centrifugalized by. FicollHypaque. Wash three:times with RPMI-1640 medium. Resuspend cell at 5 ?106/ml. Add 1% PHA and culture for 48h. Horvest supernatant and store at - 20℃.Measurement and Main Rcsults The levels of IL-2 in children with bronchopneumonia and normal children were (CPM) 9 538 + 3 363, 16 152 ? 3 411 (t test, P

Objective To study cerebral cortical activation preoperatively and postoperatively in patients with cervical spondylotic myelopathy (CSM), and discuss effect of the surgery on cortical reorganization in functional recovery.Methods 19 cases with CSM intrial group underwent cervical vertebra canal decompression surgery according to clinical routine.Cases in trial group completed clinical assessment using the modified Japanese Orthopaedic Association Scores (mJOA) prior to decompression and 12 months following surgery, and underwent cerebral functional MRI and conventional MRI in the same time.19 controls also carried out cerebral functional MRI and conventional MRI.All subjects performed a finger-tapping paradigm with right hand during processing functional MRI.The imagings and data of trial group were divided into preoperative group and postoperative group in accordance with the time of completed and than analysed.Results The mJOA score of postoperative group was increased significantly (P<0.001).Cortical volume of activation (VOA) of preoperative group was significantly higher than the control group (P<0.05).VOA was lower in the postoperative group, but still significantly higher than the control group (P<0.05).The preoperative group was able to detect and activate the signal only in the left precentral gyrus.The postoperative group was able to detect and activate the signal in the l left postcentral gyrus, the premotor area and the supplementary motor area, and the right cerebral cortex could also detect a small amount of activation signal.Conclusion CSM patients undergo cerebral cortical remodeling, causing sensory and motor function activation areas to expand and shift.The changes of cortical reorganization after cervical vertebra canal decompression surgery are associated with functional recovery.The surgical treatment may promote the compensating cortical reorganization.

Objectives To investigate the expression features and roles of the costimulatory molecules and T lymphocyte subsets from patients with chronic nephritis.Methods The expression of the costimulatory molecules CD 28 and CD 137 on PBMC (peripheral blood mononuclear cells) and T lymphocyte subsets from 52 patients with chronic nephritis were studied by immunophenotyping and flow cytometry analysis.Results The T lymphocyte subsets from patients with chronic nephritis showed an obvious imbalance with reversing CD 4/CD 8 ratio,decreasing CD + 4T cells and increasing CD + 8T cells.The expression of the costimulatory molecule CD 28 on T cells was significantly lower compared with normal controls (P

The repair of severed tendons within the digital sheath constitutes a complicated surgical problem. The injury and adhesion of the tendons are an important factor for functional return in the hand. On the basis of experimental study, 7 cases of injuries of flexor digitalis tendons have been treated by using silastic artificial tendons since 1982. During the operation the tendon graft was ensheathed in a porous filmy silastic tube, including the sites of anastomosis. This technique can prevent adhesion between tendon and its surrounding tissues. The operation can be performed in one stage. The operative procedure and its indication, reconstruction of the pulley system and the gliding function of the tendon were discussed.

Carcinoembryonic Antigen ; metabolism ; Carcinoma in Situ ; immunology ; pathology ; virology ; Carcinoma, Ductal, Breast ; immunology ; pathology ; virology ; Cervical Intraepithelial Neoplasia ; immunology ; pathology ; virology ; DNA, Viral ; analysis ; Diagnosis, Differential ; Female ; Human papillomavirus 16 ; genetics ; isolation & purification ; Humans ; Ki-67 Antigen ; metabolism ; Uterine Cervical Dysplasia ; immunology ; pathology ; virology ; Uterine Cervical Neoplasms ; immunology ; pathology ; virology

Objective To understand the current status of health knowledge demands and its associated factors in general population. Methods A questionnaire-based survey by convenience sampling method was carried out in 700 subjects aged over 15 years old in October 2014. Information of demographics and health knowledge demands were collected. Data was analyzed using SPSS 17.0. Results A total of 653 subjects were responded, with a response rate of 93.3%. Respondents in male and female were 339 (51.9%) and 314 (48.1%) respectively. There were no differences among age , educational levels and self-reported health status between the two genders 86.6% of the participants showed that they had interests in health knowledge. Educational level and age were the factors associated with the interests. 65.8% and 72.3% of people want to get practical health knowledge of western and traditional Chinese medicine respectively. The top concern of health issues were cardiovascular and cerebrovascular diseases. For preferred ways of learning health knowledge , television is the favorite one (44%), especially in elderly (over 63.8%). A relative high option rate was newspaper and magazine in all age groups. However, young people showed that they preferred the ways of the internet and Wechat (about 50%). For health disseminators, the participants in all age had greatest trust in experts in large hospitals (over 60%). The second option was retired old experts. The experienced patients were convinced in some degree and increased with age. Conclusions In future, health education in general population should considered the characteristics of the population and the applicability of knowledge. Besides , it is necessary to integrate traditional Chinese medicine with Western medicine, traditional media with new media, also authoritative experts and living example.

[Objective]To verify whether or not the modified open-door laminoplasy preserving semispinalis cervicis insert into axis is effective in preventing postoperative axial symptom and maintaining postoperative cervical sagittal alignment.[Method]Since March 2002 to December 2003,forty-eight patients underwent modified open-door laminoplasy preserving semispinalis cervicis insert into Axis,among them,thirty-two patients who were followed up for more than 2 years were included in this study.Preoperative and postoperative JOA score,degree of axial symptom,ranges of neck motion,cervical curvature index were recorded and compared.[Result]Preoperative and postoperative JOA score was(9.04?2.9)and(12.5?3.1)respectively,with recovery rate as(44.9?26.9).There was significant difference between preoperative evident axil symptom rate(46.8%)and postoperative evident axil symptom rate(18.7%),while no significant difference between preoperative and postoperative ranges of neck motion,cervical curvature index was seen.[Conclusion]Modified open-door laminoplasy is less invasive to the posterior extensor mechanism especially the semispinalis cervicis than conventional open-door laminoplasy.This new procedure is effective in preventing postoperative morbidities often seen after conventional laminoplasy with adequate decompression of the spinal cord.