1.Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria.
Bin LI ; Guan-lin XIE ; A SOAD ; J COOSEMANS
Journal of Zhejiang University. Science. B 2005;6(6):496-501
Four rhizobacteria selected out of over 500 isolates from rhizosphere of the vegetables in China were further studied for suppression of the root-knot nematode and soil-borne fungal pathogens in laboratory and greenhouse in Belgium. They were identified as Brevibacillus brevis or Bacillus subtilis by Biolog test and partial 16s rDNA sequence comparison. They not only inhibited the radial growth of the root-infecting fungi Rhizoctonia solani SX-6, Pythium aphanidermatum ZJP-1 and Fusarium oxysporum f.sp. cucumerinum ZJF-2 in vitro, but also exhibited strong nematicidal activity by killing the second stage larvae of Meloidogyne javanica to varying degrees in the greenhouse. The toxic principles of bacterium B7 that showed the highest juvenile mortality were partially characterized. The active factors were heat stability and resistance to extreme pH values. B7 used either as seed dressing or soil drench significantly reduced the nematode populations in the rhizosphere and enhanced the growth of mungbean plants over the controls in the presence or absence of R. solani.
Alphaproteobacteria
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physiology
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Animals
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Pest Control, Biological
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methods
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Phaseolus
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microbiology
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parasitology
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Seeds
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microbiology
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parasitology
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Survival Analysis
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Survival Rate
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Symbiosis
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physiology
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Tylenchoidea
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microbiology
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pathogenicity
2.Hepatoprotective effects of Astragalus kahiricus root extract against ethanol-induced liver apoptosis in rats.
Rasha M ALLAM ; Dina A SELIM ; Asser I GHONEIM ; Mohamed M RADWAN ; Salwa M NOFAL ; Amani E KHALIFA ; Ola A SHARAF ; Soad M TOAIMA ; Aya M ASAAD ; Nadia A EL-SEBAKHY
Chinese Journal of Natural Medicines (English Ed.) 2013;11(4):354-361
The hepatoprotective activity of the ethanol extract of Astragalus kahiricus (Fabaceae) roots against ethanol-induced liver apoptosis was evaluated and it showed very promising hepatoprotective actions through different mechanisms. The extract counteracted the ethanol-induced liver enzymes leakage and glutathione depletion. In addition, it demonstrated anti-apoptotic effects against caspase-3 activation and DNA fragmentation that were confirmed by liver histopathological examination. Moreover, the phytochemical study of this extract led to the isolation of four cycloartane-type triterpenes identified as astrasieversianin II (1), astramembrannin II (2), astrasieversianin XIV (3), and cycloastragenol (4). The structures of these isolates were established by HRESI-MS and 1D and 2D NMR experiments. The antimicrobial, antimalarial, and cytotoxic activities of the isolates were further evaluated, but none of them showed any activity.
Animals
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Apoptosis
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drug effects
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Astragalus Plant
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chemistry
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Caspase 3
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metabolism
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Chemical and Drug Induced Liver Injury
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drug therapy
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genetics
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physiopathology
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prevention & control
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DNA Fragmentation
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drug effects
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Ethanol
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toxicity
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Female
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Humans
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Liver
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cytology
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drug effects
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enzymology
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Plant Extracts
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administration & dosage
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Plant Roots
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chemistry
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Protective Agents
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administration & dosage
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Rats
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Rats, Sprague-Dawley
3. Pomegranate peel extract lessens histopathologic changes and restores antioxidant homeostasis in the hippocampus of rats with aluminium chloride-induced Alzheimer's disease
Steve HARAKEH ; Wafaa RAMADAN ; Wafaa RAMADAN ; Mohammed AL MUHAYAWI ; Mohammed AL MUHAYAWI ; Steve HARAKEH ; Soad AL JAOUNI ; Shaker MOUSA ; Soad AL JAOUNI ; Khalid HAKEEM
Asian Pacific Journal of Tropical Medicine 2020;13(10):456-463
Objective: To investigate the neuroprotective efficacy of pomegranate and ellagic acid on the histopathological changes in the hippocampus of an aluminium chloride (AlCl3) induced rat model of Alzheimer's disease. Methods: Sprague Dawley rats were divided into 4 groups (n=10 each): Group I : serving as negative control; Group II, Alzheimer model, induced by administration of 17 mg/kg bw AlCl3; Group III, administered the same dose of AlCl3 with 50 mg/kg of pomegranate peel extract and Group IV : administered ellagic acid (50 mg/kg) in addition to the same dose of AlCl3. The medication given to all groups continued for 28 days. All were given the compounds by gastric gavage. Radial arm maze test, hippocampus antioxidant markers, histopathology of the dentate gyrus, and CA3 of the hippocampus were evaluated. Results: Rats treated with pomegranate peel extract exposed to radial arm maze test showed less number of errors and reduced time needed to reach the criterion. There was an increase in the levels of glutathione, catalase, and total antioxidant capacity and decreased lipid peroxidation products. Histopathological features in dentate gyrus and CA3 as apoptosis and chromatolysis of pyramidal cells and granular layer, respectively, were decreased. Alzheimer characteristic neurofibrillary tangles and senile plaques were reduced. Treatment with ellagic acid ameliorated the pathological results but to a statistically lower level. Conclusions: Pomegranate peel extract alleviates memory deficit and restores antioxidant homeostasis following degenerative changes in the hippocampus induced by aluminium chloride in rats..
4. Moringa oleifera leaf ethanol extract ameliorates lead-induced hepato-nephrotoxicity in rabbits
Nancy B. MOHAMED ; Amira H. MOHAMED ; Nashwa A. ABU-AITA ; Soad M. NASR ; Somia A. NASSAR ; Somia A. NASSAR ; Kawkab A. AHMED
Asian Pacific Journal of Tropical Biomedicine 2020;10(6):263-272
Objective: To evaluate the effect of Moringa oleifera leaf ethanol extract as an adjunct treatment on lead acetate induced hepato-nephrotoxicity in rabbits. Methods: Thirty-six male New Zealand White rabbits were assigned into two main groups. The first group (14 rabbits) served as normal control. The secondgroup (22 rabbits) was administered orally with lead acetate at a dose of 40 mg/kg/day, 5 days/week for 8 weeks. At the 4th and the 8th week of treatment, 6 animals (3 animals at each period) of the second group were sacrificed while the remaining animals (16 rabbits) were assigned randomly into 2 subgroups (8 rabbits each): treated and non-treated. The first subgroup was orally given 1 mL phosphate-buffered saline for further 4 weeks while the second subgroup was administered orally with Moringa oleifera leaf ethanol extract at a dose of 400 mg/kg/day for the same period. Blood samples were collected to determine hematological and serum biochemical indices. Tissue specimens were collected from the liver and kidney for evaluation of the oxidant/antioxidant markers and for histopathological examinations. Results: Lead acetate exposure decreased the mean body weight gain, hematocrit, hemoglobin, mean corpuscular volume, and lymphocytes count. Moreover, it markedly increased counts of monocytes and platelets, serum enzyme activity, levels of creatinine, total cholesterol, triglycerides, and low-density lipoprotein cholesterol. Malondialdehyde level was markedly increased while the reduced glutathione content was significantly decreased in liver tissue of lead intoxicated-rabbits. Histopathological alterations were also noticed in the liver and kidney of lead intoxicated rabbits. Moringa oleifera leaf ethanol extract significantly improved hematological and serum biochemical parameters and histopathological structure of the liver and kidney. Conclusions: Moringa oleifera leaf ethanol extract ameliorates hemato-biochemical and histopathological alterations caused by lead acetate and improveshepatic and renal functions.
5. Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model
Attia H. ATTA ; Samar M. MOUNEIR ; Soad M. NASR ; Doaa SEDKY ; Amany M. MOHAMED ; Shimaa A. ATTA ; Hassan Mohamed DESOUKY
Asian Pacific Journal of Tropical Biomedicine 2019;9(3):98-108
Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera (M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of 5 rats each were used: normal control group with distilled water, model group, two groups with M. oleifera seeds (100 and 200 mg/kg), two groups with propolis (50 and 100 mg/kg), one group with concurrent administration of both, and one group with prednisolone (reference drug). Macro-and microscopic picture, ulcer index and lesion scores, oxidative markers, inflammatory mediators, in vitro activity of the inflammatory enzymes and 1, 1-diphenyl-2-picrylhydrazyl free radicals scavenging activity were evaluated. The phytochemical constituents of both extracts were explored by GC-MS analysis. Results: Both treatments modulated the macro-and microscopic picture, decreased the ulcerative index, lesion score, oxidative markers and inflammatory mediators, and inhibited the COX-1 and COX-2 enzymes. Propolis appeared to be powerful free radicals scavenger. A powerful synergistic effect of both treatments in modulating the course of the disease was reported. GC-MS analysis of methanol extract of M. oleifera seeds and propolis revealed the presence of 50 and 34 compounds, respectively. Conclusions: M. oleifera seeds and propolis methanol extracts have modulated the course of acetic acid-induced ulcerative colitis. Moreover, both treatments induce a good synergistic effect against the disease. Isolation of the active constituents is recommended.
6. Synergetic effect of Egyptian propolis in immunization of BALB/c mice against bovine cysticercosis
Omnia Mohamed KANDIL ; Somia Ayesh NASSAR ; Soad Mohamed NASR ; Hatem Abdel Mawgoud SHALABY ; Seham HENDAWY ; Faragalla Mohamed EL MOGHAZY ; Faragalla Mohamed EL MOGHAZY
Asian Pacific Journal of Tropical Biomedicine 2015;5(4):324-330
OBJECTIVE: To evaluate the synergetic effect of an ethanolic extract of Egyptian propolis in immunization of BALB/c mice with Taenia saginata (T. saginata) crude antigen against bovine cysticercosis, with reference to its effects on liver and kidney functions. METHODS: Sixty female mice BALB/c strain weighing 20 to 25 g and 6-8 weeks old were randomly allocated into six groups of ten mice each. Mice in groups 1 and 2 (G1 and G2) were immunized intraperitoneally with 100 μg of T. saginata crude antigen in 100 μL phosphate buffer saline emulsified in Freund's adjuvant. Besides, the mice in G2 were administered with propolis extract simultaneously with immunization. Control mice were either administered with propolis extract (G3) or injected with the same volume of phosphate buffer saline emulsified in Freund's adjuvant (G4). The mice in G5 were non-immunized infected control while, those in G6 were non-immunized non-infected control. Two weeks after the last immunization, each mouse was challenged intraperitoneally with 5 000 oncospheres except those of G6. Ethanolic extract of propolis was prepared at a dose 50 mg/kg body weight. RESULTS: After 24 weeks of challenge, the mice in G2 showed the highest level of protection (100%), with no cyst being detected rather than mice in G1 (33.3% protection). Additionally, the ELISA results, in this study, showed higher antibody titer in G2 with reduction the alteration in liver and kidney functions compared to G1. CONCLUSIONS: Egyptian propolis could increase the level of protection against experimental challenge infection with T. saginata eggs when administered simultaneously with immunization. Furthermore, it could enhance the production of antibodies to immunized antigen and decrease the alteration in liver and kidney functions.
7. Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model
Attia H. ATTA ; Abduljalil H. ALMAWERI ; Mostafa A. SHALABY ; Soad M. NASR ; Doaa SEDKY ; Amany M. MOHAMED ; Hassan M. DESOUKY
Asian Pacific Journal of Tropical Medicine 2018;11(7):423-429
Objective: To evaluate the potential antioxidant and hepatoprotective effects of n-hexane, dichloromethane(DCM), ethyl acetate(EtOAc), n-butanol and aqueous fractions of Moringa oleifera(M. oleifera) leaves methanol extract against carbon tetrachloride(CCl
8. Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding, leukogram, protein profile and ileum histopathology
Hamdy SOUFY ; Soad M. NASR ; Tamer H. ABD EL-AZIZ ; Fathia A.M. KHALIL ; Hala A.A. ABOU ZEINA ; Nadia M. EL-BEIH ; Youssef F. AHMED
Asian Pacific Journal of Tropical Medicine 2017;10(3):253-262
Objectives To investigate the activity of Egyptian propolis extracts (ethanol and water) on cryptosporidiosis in experimentally infected dexamethasone-immunosuppressed rats. Methods A total of 180 male rats (190–220) g BWt were randomly divided into 9 equal groups (G1–G9). Groups of rats were kept as (G1): normal control, (G2–G9): immunosuppressed with dexamethasone and (G3-G9): infected with Cryptosporidium oocysts. Rats from (G4–G9) were given orally ethanol and water extract of propolis (at a dose of 50 mg/kg BWt) and nitazoxanide (standard anti-cryptosporidial drug at a dose of 100 mg/kg BWt) to infected rats with different regimes. Faecal pellets were collected from all groups to monitor oocysts shedding from the 2nd to the 15th day post infection. At the end of the experiment, blood was collected from all groups for determination of leukogram and serum proteins. Ileum specimens were also examined histopathologically. Results The highest reduction of oocysts shedding in faecal samples was 88% in rats prophylactically treated with propolis ethanol extract at the 4th dpi, and in rats prophylactically treated with water extract of propolis, was 91% at the 6th dpi. There was a marked increase in neutrophils count and α
9. Antioxidant lipoxygenase inhibitors from the leaf extracts of Simmondsia chinensis
Wael Mostafa ABDEL-MAGEED ; Wael Mostafa ABDEL-MAGEED ; Soad Abdel Latief Hassan BAYOUMI ; Hanaa Mohamed SAYED ; Awwad Abdoh Radwan SALAMA ; Mounir Mohamed SALEM-BEKHIT ; Sherif Hussein ABD-ALRAHMAN
Asian Pacific Journal of Tropical Medicine 2014;7(S1):S521-S526
Objective: To isolate and identify chemical constituents with antioxidant and lipoxygenase inhibitory effects of the ethanolic extract of Simmondsia chinensis (Jojoba) leaves. Methods: The alcoholic extract was subjected to successive solvent fractionation. The antioxidant active fractions (chloroform, ethyl acetate and aqueous fractions) were subjected to a combination of different chromatographic techniques guided by the antioxidant assay with DPPH. The structures of the isolated compounds were elucidated on the basis of spectroscopic evidences and correlated with known compounds. The antioxidant activity was assessed quantitively using DPPH and β-carotene methods. The inhibitory potential against enzyme lipoxygenase was assessed on soybean lipoxygenase enzyme. Results: Ten flavonoids and four lignans were isolated. Flavonoid aglycones showed stronger antioxidant and lipoxygenase inhibitory effects than their glycosides. Lignoid glycosides showed moderate to weak antioxidant and lipoxygenase inhibitory effects. Conclusions: A total of 14 compounds were isolated and identified from Simmondsia chinensis; 12 of them were isolated for the first time. This is the first report that highlights deeply on the phenolic content of jojoba and their potential biological activities and shows the importance of this plant as a good source of phenolics in particular the flavonoid content.