Objective To research the action mechanism of Chinese FormulaXuetongling on MDA-MB-231 human breast cancer cell invasion.Methods Transwell Invasion assay was applied to investigate the inhibitory effects on cancer cell invasion ofXuetongling extract in different concentrations;MMP-9 secretion activity was detected by zymography assay after the treatment of XTL extract in MDA-MB-231;Western blot was used to detect the effect of XTL extract on MMP-9 protein expression in MDA-MB-231.ResultsXuetongling extract in different concentrations significantly suppressed the cell invasion, MMP-9 secretion and MMP-9 protein expression in dose dependent manner.Conclusion The inhibitory effect ofXuetongling on MDA-MB-231 cell invasion may be due to the down-regulation of both MMP-9 secretion and MMP-9 protein expression.

Objective To explore the cause of repeated offline of files in the database of No.1 Military Project and find the method to solve the problem. Methods The abnormal offline of files in database is not by chance,and its document in oracle is not very clear. Using the knowledge from internet,the fault was repeatedly simulated on test machine and the source of the fault was found. Results Solving plans and countermeasures were put forward. Conclusion The database of the No.1 Military Medical Project is the core of military hospital work,so its importance is self-evident. The technical support of No. 1 Military Project is strengthened,the right maintenance direction of the database is pointed out as well as the right solution to deal with the problem.

No abstract available.
Animals ; Neurons* ; Rats*

No abstract available.
Animals ; Growth Hormone* ; Prolactin* ; Rats*

No abstract available.
Animals ; Rats* ; Somatotrophs*

No abstract available.

BACKGROUND: Unplanned extubation (UE) of patients requiring mechanical ventilation in an intensive care unit (ICU) is associated with poor outcomes for patients and organizations. This study was conducted to assess the clinical features of patients who experienced UE and to determine the risk factors affecting reintubation after UE in an ICU. METHODS: Among all adult patients admitted to the ICU in our institution who required mechanical ventilation between January 2011 and December 2013, those in whom UE was noted were included in the study. Data were categorized according to noninvasive or invasive management after UE. RESULTS: The rate of UE was 0.78% (the number of UEs per 100 days of mechanical ventilation). The incidence of self-extubation was 97.2%, while extubation was accidental in the remaining patients. Two cases of cardiac arrest combined with respiratory arrest after UE were noted. Of the 214 incidents, 54.7% required invasive management after UE. Long duration of mechanical ventilation (odds ratio [OR] 1.52; 95% confidence interval [CI] 1.32-1.75; p = 0.000) and high ICU mortality (OR 4.39; 95% CI 1.33-14.50; p = 0.015) showed the most significant association with invasive management after UE. In multivariate analysis, younger age (OR 0.96; 95% CI 0.93-0.99; p = 0.005), medical patients (OR 4.36; 95% CI 1.95-9.75; p = 0.000), use of sedative medication (OR 4.95; 95% CI 1.97-12.41; p = 0.001), large amount of secretion (OR 2.66; 95% CI 1.01-7.02; p = 0.049), and low PaO2/FiO2 ratio (OR 0.99; 95% CI 0.98-0.99; p = 0.000) were independent risk factors of invasive management after UE. CONCLUSIONS: To prevent unfavorable clinical outcomes, close attention and proper ventilatory support are required for patients with risk factors who require invasive management after UE.
Adult ; Heart Arrest ; Humans ; Incidence ; Intensive Care Units* ; Critical Care* ; Mortality ; Multivariate Analysis ; Respiration, Artificial ; Risk Factors

PURPOSE: Our objective was to determine the effect of erythromycin (EM) in improving gastrointestinal motility in subtotal gastrectomized patients. We used radio-opaque Kolomarks as an objective method. We conducted a prospective, controlled clinical trial study of 24 patients. METHODS: All patients underwent subtotal gastrectomy with 3 capsules containing Kolomarks (20 markers per 1 capsule) in the remnant stomach before anastomosis. From the day of the operation to the 2nd postoperative day, patients in the EM group began receiving 200 mg of EM intravenously for 30 minutes continuously. We counted the number of Kolomarks in the stomach, passed by stomach, in rectum, and in stool with serial simple abdominal X-ray films on the first postoperative day up to the 7th postoperative day. RESULTS: The study population included 14 patients in the control group and 10 patients in the EM group. The two study groups were compared in terms of their characteristics including age, gender, past medical history, cancer stage, and operation type. No significant differences were found for the demographics between the two groups. We only found a significant difference for the number of Kolomarks passed by the stomach on the 3rd postoperative day (P = 0.026). CONCLUSION: Our results demonstrated that 200 mg of EM intravenous infusion during the postoperative period induced rapid gastric emptying, although it did not improve gastrointestinal motility for the entire gastrointestinal tract in subtotal gastrectomized patients.
Capsules ; Demography ; Erythromycin ; Gastrectomy ; Gastric Emptying ; Gastric Stump ; Gastrointestinal Motility ; Gastrointestinal Tract ; Humans ; Infusions, Intravenous ; Postoperative Period ; Prospective Studies ; Rectum ; Stomach ; X-Ray Film

Breast Feeding ; Canada ; Caregivers ; education ; Child ; Cross Infection ; prevention & control ; Feeding Behavior ; Humans ; Infant, Newborn ; Infant, Premature ; Intensive Care Units, Neonatal ; organization & administration ; Intensive Care, Neonatal ; methods ; Neonatal Nursing ; methods ; Parents ; education ; Practice Patterns, Physicians'

This study was performed to investigate the effect of progesterone (P) on the estrogen-induced proliferation of mammotropes in cultures of normal rat pituitary cells. Dispersed pituitary cells from male rats were cultured in Dulbecco's Modified Eagle's medium containing striped 10% fetal bovine serum for 6 days. Part of cells were treated with 10(-9) M 17beta-estradiol with or without P (10(-6) M). For the simultaneous detection of mitotic cells and mam-motropes, bromodeoxyuridine (BrdU) labeling and double immunocytochemical staining for BrdU and prolactin were performed. The ratio of BrdU-labeled cells among mammotropes (BLI) was increased by 6.3-fold after 6 day treatment with estradiol, which was partly suppressed by 2.3-fold by additional P treatment. The percentage of mammotropes among pituitary cells was increased by 1.5-fold by estradiol treatment, which was suppressed by 1.1-fold after coadminis-tration of progesterone. Thus, coadmistration of progesterone could suppress the estradiol-induced effect on the BLI and the proportion of mammotropes by 75% and 80%, respectively. In addition, apoptotic ratio of mammotropes was not changed by hormone treatment. The present data demonstrated that progesterone inhibits estradiol-induced proliferation of mammotropes, which may contribute to the decrease in the proportion of mammotrope. The indirect modulatory effect of progesterone on the estradiol-induced increase in the prolactin secretion in the rat pituitary was suggested.
Animals ; Bromodeoxyuridine ; Estradiol ; Estrogens* ; Humans ; Male ; Progesterone* ; Prolactin ; Rats*