Objective To explore the expression and cilinical significance of UCH37 in colon cancer, and detect the effects and underlying mechanism of UCH37 on cell proliferation and cell apoptosis of colon cancer cell lines. Methods Expressions of UCH37 were analyzed by immunohistochemistry in colon cancer patients. Spearman′s rank test was conducted to analyze the clinical relevance of UCH37 in colon cancer. QPCR was conducted to detect the expression of UPS39 in colon cancer cell lines and NCM460 cells.CCK-8,flow cytometry, co-immunoprecipitation were conducted to detect the effects of UCH37 on cell proliferation,cell apoptosis. And ubiquitinated level of MIF. Results Compare to adjacent tissues,immunohistochemistry and chi square analysis revealed that the positive rate of UCH37 was upregulated in colon cancer tissues.Spearman rank correlation showed that positive UCH37 expression was significantly associated with TNM stage and cell differentiation of colon cancer patients. CCK-8 results showed cell proliferation in colon cancer cells transfected with UCH37 NC was promoted and cell apoptosis in colon cancer cells transfected with UCH37 NC was inhibited compared with cells transfected UCH37 siRNAs;furthermore,compared to cells transfected lentiviral vector carrying pLenti6.3.cell proliferation in colon cancer cells transfected with lentiviral vector carrying UCH37 was promoted and cell apoptosis in colon can-cer cells transfected with lentiviral vector carrying UCH37 was inhibited.Co-IP showed MIF could be deubiquitinat-ed by UCH37 in colon cancer cells. Conclusion UCH37 expression is upregulated in colon cancer,and UCH37 could promote cell proliferation of colon cancer cells by deubiquitinating MIF.

Objective:To explore the effect of ear acupoint pressure pills combined with psychological intervention on the psychological status and quality of life of patients with rectal cancer undergoing chemotherapy, provide the reference for patients with rectal cancer undergoing chemotherapy.Methods:A total of 220 patients with rectal cancer who received chemotherapy in Mianyang Central Hospital from August 2017 to October 2019 were selected as the research subjects. Random number table method was used to divide 220 patients with rectal cancer into observation group (110 cases) and control group (110 cases). Observation group was given ear acupoint pressure pills and psychological intervention, while control group was given psychological intervention only. The Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were used to evaluate mental state; the European Cancer Research and Treatment Organization (EORTC) Quality of Life Questionnaire-Core 30 (QLQ-C30) was used to evaluate the quality of life.Results:There was no significant difference in the score of SAS, SDS and quality of life before intervention between the two groups( P>0.05). The SAS and SDS scores of the observation group after intervention were 46.47±4.00 and 46.33±3.17, which were lower than the control group (52.87±4.02, 51.03±4.18), and the differences were statistically significant ( t values were 11.836, 9.396, P<0.05). After intervention, the scores of physical function, physiological function, psychological function and social function in the observation group were 61.38±7.46,65.28±7.04, 62.20±8.15 and 75.21±9.05, respectively, which were all higher than those in the control group (56.41±6.01, 57.25±4.31, 57.26±7.94 and 64.82±8.40), and the differences were statistically significant ( t values were 4.554 -10.203, P<0.05). Conclusions:Ear acupoint pressure pills combined with psychological intervention can improve the psychological status and quality of life of patients with rectal cancer treated with chemotherapy and improve treatment compliance.