OBJECTIVETo study the chemical constituents of Dolomiaea souliei.

METHODVarious chromatographic techniques were adopted to separate the constituents, and the spectrum analysis was made to identify their structures.

RESULTSeventeen compounds were isolated and identified as: dehydrocostus lactone (1), costunolide (2), mokko lactone (3), santamarine(4), reynosin (5), 4alpha-hydroxy-4beta-methyldihydrocostol (6), sulfocostunolide A (7), beta-costic acid (8), beta-cyclocostunolide (9), vladinol A (10), ursolic acid (11), betulinic acid (12), betulin (13), dibutyl terephthalate (14), dibutyl phthalate (15), uridine (16), and emodin (17).

CONCLUSIONCompounds 6-9 and 12-17 were obtained from this genus for the first time, and compound 11 was obtained from this plant for the first time.

4-Butyrolactone ; analogs & derivatives ; chemistry ; Asteraceae ; chemistry ; Emodin ; chemistry ; Lactones ; chemistry ; Sesquiterpenes ; chemistry ; Triterpenes ; chemistry

AIM: To investigate chemical constituents of Spatholobus suberectus Dunn. METHODS: Isolation and purification were carried out by column chromatographic methods. Compounds were characterized based on their physical characteristics and spectra data. RESULTS: Seventeen compounds were isolated from ethanol extract of S. suberectus. The structures were elucidated as prestegane B (1), (2R, 3R)-buteaspermanol (2), (+)-medioresinol (3), (2R, 3R)-3,7-dihydroxyflavanone (4), benzeneethanol (5), 4, 7, 2'-trihydroxy-4'-methoxyisoflavanol (6), naringenin (7), blumenol A (8), protocatechuic acid ethyl ester (9), liquiritigenin (10), 7, 4'-dihydroxy-8-methoxy-isoflavone (11), 3, 5, 7, 3', 5'-pentahydroxyflavanone (12), protocatechuic acid (13), glycyroside (14), 8-methylretusin-7-O-β-D-glucopyranoside (15), 3, 3', 4', 5, 6, 7, 8-heptahydroxyflavan (16), and dulcisflavan (17). CONCLUSION All compounds are firstly isolated from the title plant and compounds 1, 3 were isolated from the Spatholobus genus for the first time.
4-Butyrolactone ; analogs & derivatives ; chemistry ; isolation & purification ; Fabaceae ; chemistry ; Lignans ; chemistry ; isolation & purification ; Molecular Structure ; Plant Extracts ; chemistry

OBJECTIVETo compare 39 root samples of Angelica sinensis and A. acutiloba from China and Japan for the quality control of Chinese Angelica.

METHODAn HP-5 (0.32 mm x 30 m, 0.25 microm) column was used for the GC-MS analysis. The oven temperature was programmed from 120 degrees C to 280 degrees C at a rate of 5 degrees C x min(-1). Using principal component analysis, cluster analysis, and discriminant analysis on the sample fingerprints for chemical pattern recognition research.

RESULTZ-Ligustilide was the key principle distinguishing Chin samples from Japan. Moreover, using discriminant analysis, seven samples (four of A. sinensis, three of A. acutiloba) were validated. All samples tested were successfully classified according to their species origin.

4-Butyrolactone ; analogs & derivatives ; chemistry ; Angelica sinensis ; chemistry ; Cluster Analysis ; Discriminant Analysis ; Gas Chromatography-Mass Spectrometry ; methods ; Principal Component Analysis

Bioactivity of Danggui is linked to the content of ligustilide, but the relationship between ligustilide with herb shape, cultivating areas and plant species is still unknown. The relationship was investigated by quantifying on the amounts of Z-ligustilide and E-ligustilide by HPLC-DAD-MS method, and then comparing the content of ligustilides (the sum of Z-ligustilide and E-ligustilide) among forty-four various "Danggui" samples containing thirty Chinese Danggui (CDG), six Japanese Danggui (JDG), four Korea Danggui (KDG) and four European Danggui (EDG). Results showed that the content of ligustilides in CDG samples (Angelica sinensis) was in the range of 5.63-24.53 mg x g(-1) with the mean of 11.02 mg x g(-1) (n = 30). Ligustilides amounts were varied among samples cultivated in different areas in China, i. e. 13.90 mg x g(-1) (n = 6) in Yannan, 12.51 mg x g(-1) (n = 6) in Sichuan and 10.04 mg x g(-1) (n = 13) in Gansu. It was also found that ligustilides content was related to the shape, color and fragrance of herb, e. g. the relative larger amount of ligustilides was in the small main root, long rootlet and perfumed sample. Further, ligustilides contents were estimated to be 1.00 mg x g(-1) (n = 6) in JDG samples (A. acutiloba and A. acutiloba var. sugiyamae) and 2.78 mg x g(-1) (n = 2) in EDG samples (lovage root, Levisticum officinale). However, ligustilides could not be detected in the four KDG samples (A. gigas) and two EDG samples (angelica root, A. archangelica). It has been concluded that ligustilide is significant variant among plant species, which may result in the variety of bioactivity and therapeutic effect.
4-Butyrolactone ; analogs & derivatives ; analysis ; Angelica sinensis ; chemistry ; China ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Geography ; Quality Control

Ligustilide is contained highly or around 1% in such umbelliferous plants as Angelica sinensis and Ligusticum chuanxiong, is one of main bioactive constituents. It shows many pharmacological activities related to their efficacy. At present, ligustilide has attracted extensive attention and more and more studies have been reported, indicating that it is a promising compound. This essay summarizes the progress of pharmacological effects of ligustilide on neuroprotection, vasodilatation, anti-caner and anti-tumor, analgesia and anti-inflammation, and pharmacokinetics including absorption, distribution, metabolism and excretion, providing basis for further studies and development of ligustilide.
4-Butyrolactone ; analogs & derivatives ; pharmacokinetics ; pharmacology ; Angelica sinensis ; chemistry ; Animals ; Drugs, Chinese Herbal ; pharmacokinetics ; pharmacology ; Humans ; Ligusticum ; chemistry

OBJECTIVETo study the chemical constituents of Ligusticum chuanxiong.

METHODThe constituents were separated and purified by column chromatography with silica gel and ODS, and identified by NMR, MS spectral data.

RESULTEight compounds were isolated and identified as Ligustilide(I), 4,5-dihydro-3-butylphthalide(II), Z, Z'-6,6',7,3 alpha-diligustili-de(III), Z-6,8',7,3'-diligustilide(IV), Z'-3,8-dihydro-6,6'7,3' alpha-diligustilide(V), 4-hydroxy-3-butylphthalide(VI), ferulic acid(VII) and Xiongterpene(VIII).

CONCLUSIONCompound VIII is a new compound.

4-Butyrolactone ; analogs & derivatives ; chemistry ; isolation & purification ; Coumaric Acids ; chemistry ; isolation & purification ; Ligusticum ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry ; Rhizome ; chemistry ; Terpenes ; chemistry ; isolation & purification

OBJECTIVETo investigate the chemical constituents of Hemistepta lyrata.

METHODThe constituents of the EtOAc-soluble portions of the 95% ethanol extract were isolated and purified by means of chromatography. Compounds were identified by their physical characteristics and spectral features.

RESULTFive compounds were isolated and identified as caffeic acid (1), tracheloside (2), uracil (3), 8-carboxymethyl-p-hydroxycinnamic acid (4), and 3-O-p-coumaroylquinic acid (5).

CONCLUSIONCompounds 1-5 were isolated from this genus for the first time.

4-Butyrolactone ; analogs & derivatives ; chemistry ; isolation & purification ; Asteraceae ; chemistry ; Caffeic Acids ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Uracil ; chemistry ; isolation & purification

A simple and quick method is described for the determination of ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A and ligustilide in rhizomes of Ligusticum chuanxiong. The 5 active ingredients in the sample was extracted using 40% ethanol and analyzed by reversed-phase high performance liquid chromatography (HPLC). Chromatography separation was performed using Agilent 1100 series HPLC system with a Symmetry C18 column and gradient elution with a mixture of three solvents : solvent A, acetonitrile, solvent B, methanol and solvent C, 1% aqueous acetic acid, 0 min to 5 min A: B: C 20: 40: 40, 5 min to 30 min A: B: C 60 to 100 : 0 : 40 to 0. The effluent was monitored using a VWD detector set at 321 nm (0-4.3 min) and 275 nm (4.31-30 min). The flow rate was set at 1 mL x min(-1) and the injection volume was 10 microL. The column temperature was maintained at 35 degrees C. The calibration curve was linear (r > or = 0.99) over the tested ranges. The average recovery was 94.44%-103.1% (n = 6). The method has been successfully applied to the analysis in different harvest periods of L. chuanxiong samples. In this paper, single-factor randomized block design to study the 5 components content of L. chuanxiong on ten collecting stages. For the L. chuanxiong collected from April 15th to May 30rd, the content of 5 ingredients increased primarily, and then decreased. Determine the appropriate harvest time has important significance to the promotion of the quality of L. chuanxiong.
4-Butyrolactone ; analogs & derivatives ; analysis ; Acetic Acid ; chemistry ; Acetonitriles ; chemistry ; Benzofurans ; analysis ; Chromatography, High Pressure Liquid ; methods ; Coumaric Acids ; analysis ; Ligusticum ; chemistry ; Methanol ; chemistry ; Solvents ; chemistry ; Time Factors

OBJECTIVETo find out substance basis of pharmacological activities of Angelica sinensis.

METHODChromatographic methods were used to isolate the chemical components, and spectroscopic methods were used to identify their structures.

RESULTFive compounds were isolated from the ethanol extract of the roots of Angelica sinensis. Their structures were identified as: (Z)-ligustilide, (Z)-6,7-epoxyligustilide, (Z)-6,7-cis-dihydroxyligustilide, (E)-6,7-cis-dihydroxy-ligustilide and 11-angeloylsenkyunolide F.

CONCLUSION11-angeloylsenkyunolide F was obtained from Angelica sinensis for the first time.

4-Butyrolactone ; analogs & derivatives ; chemistry ; isolation & purification ; Angelica sinensis ; chemistry ; Benzofurans ; chemistry ; isolation & purification ; Molecular Structure ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry

The present study carried out a phytochemical investigation on the root barks of Dictamnus dasycarpus Turcz, leading to the isolation and characterization of two new aromatic ring butyrolactone derivatives, dasycarpusphenol acid A (1) and dasycarpusphenol acid B (2). Their structures were elucidated by using spectroscopic techniques and HR-FAB-MS. Compounds 1 and 2 exhibited antioxidant activity, with their IC values being 28.95 and 41.76 mg·mL, respectively.
4-Butyrolactone ; chemistry ; isolation & purification ; Antioxidants ; chemistry ; isolation & purification ; Dictamnus ; chemistry ; Molecular Structure ; Plant Bark ; chemistry ; Plant Extracts ; chemistry ; isolation & purification