1.Proteomic analysis and verification of protein expression after upregulation of human CD99 in Hodgkin lymphoma cell line L428.
Xiang Ling LIN ; Qing Can SUN ; Yin LU ; Xi Qun HAN ; Tong ZHAO ; Xin Hua ZHOU
Chinese Journal of Hematology 2019;40(6):490-496
Objective: To investigate the proteins expression difference after upregulation of human CD99 in Hodgkin Lymphoma cell line, L428 cell, and verify the function of differential proteins. Methods: The differential proteins were detected by two-dimensional fluorescence difference gel electrophoresis and mass spectrometry analysis, cluster analysis was done by GOfact. Results: There were 38 proteins screened out, of which 21 proteins were positively associated with CD99, while 17 proteins were negative. Among the 38 proteins, 32 proteins participated in biological process, and 35 proteins were involved in the composition and construction. And 28 proteins participated in multifaceted biological activities including antioxidation, protein binding, catalytic activity, regulation of enzyme, signal transduction, molecular structure, regulation of translation and ion transport. Conclusions: The changes of the differential proteins, correlated with cytoskeleton, cell differentiation, signal pathway and regulating gene expression, are closely relevant to the translation between Hodgkin/Reed-Sternberg and B lymphocyte cell.
12E7 Antigen
;
Cell Line, Tumor
;
Hodgkin Disease
;
Humans
;
Proteomics
;
Up-Regulation
2.Extraskeletal osteosarcoma of kidney: report of a case.
Chinese Journal of Pathology 2014;43(3):197-198
12E7 Antigen
;
Antigens, CD
;
metabolism
;
Cell Adhesion Molecules
;
metabolism
;
Diagnosis, Differential
;
Humans
;
Kidney Neoplasms
;
metabolism
;
pathology
;
surgery
;
Male
;
Middle Aged
;
Nephrectomy
;
Osteosarcoma
;
metabolism
;
pathology
;
surgery
;
Vimentin
;
metabolism
3.Primitive neuroectodermal tumor of kidney with multiple metastases : report of a case.
Zhenyu YANG ; Xiao LI ; Minhong PAN ; Zhihong ZHANG ; Qinhe FAN
Chinese Journal of Pathology 2014;43(2):120-121
12E7 Antigen
;
Adolescent
;
Antigens, CD
;
metabolism
;
Cell Adhesion Molecules
;
metabolism
;
Diagnosis, Differential
;
Humans
;
Kidney Neoplasms
;
metabolism
;
pathology
;
surgery
;
Lymph Node Excision
;
Lymphatic Metastasis
;
Lymphoma
;
metabolism
;
pathology
;
Male
;
Neoplastic Cells, Circulating
;
Nephrectomy
;
Neuroblastoma
;
metabolism
;
pathology
;
Neuroectodermal Tumors, Primitive, Peripheral
;
metabolism
;
pathology
;
surgery
;
Synaptophysin
;
metabolism
;
Venae Cavae
;
pathology
;
Vimentin
;
metabolism
;
Wilms Tumor
;
metabolism
;
pathology
4.A case of hemangiopericytoma of the soft palate with articulate disorder and dysphagia.
Yasuyuki MICHI ; Miho SUZUKI ; Kazuto KUROHARA ; Kiyoshi HARADA
International Journal of Oral Science 2013;5(2):111-114
We report a case of hemangiopericytoma of the soft palate of 60-year-old patient, who noticed a mass of the soft palate and experienced difficulty in speaking. We found a pediculate, hard, elastic mass measuring 38 mm (cross-sectional diameter). Computed tomography (CT) scans and dynamic magnetic resonance imaging (MRI) confirmed irregularly shaped mass and revealed a heterogeneous internal composition, consistent with vascular tumors. We excised the tumor under general anesthesia. Histopathological diagnosis was based on positive immunoreactivity of CD99 and vimentin and weak, positive staining of CD34. Three and half years following tumor excision, there is no recurrence or metastasis.
12E7 Antigen
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Antigens, CD
;
analysis
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Antigens, CD34
;
analysis
;
Articulation Disorders
;
etiology
;
Cell Adhesion Molecules
;
analysis
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Deglutition Disorders
;
etiology
;
Follow-Up Studies
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Hemangiopericytoma
;
complications
;
Humans
;
Magnetic Resonance Imaging
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Male
;
Middle Aged
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Palatal Neoplasms
;
complications
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Palate, Soft
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pathology
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Tomography, X-Ray Computed
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Vimentin
;
analysis
5.Small cell malignant tumors of bone: comparison between diagnosis using core needle biopsies and surgical specimens.
Yi DING ; Yue XI ; Xiao-qi SUN ; Shu-qin MENG ; Wei-feng LIU ; Xiao-yuan HUANG
Chinese Journal of Pathology 2013;42(3):163-167
OBJECTIVETo compare the pathologic diagnosis and immunohistochemistry of small cell malignant tumors (SCMT) of bone using both core needle biopsy and surgical specimen.
METHODSSeventy-seven cases of SCMT with core needle biopsies and surgical specimens available were respectively analyzed by histologic examination and immunohistochemical study, with literature review.
RESULTSThe male-to-female ratio was 48:29. The age of the patients ranged from 6 to 73 years. The tumors studied included Ewing sarcoma/PNET (n = 38), myeloma (n = 23), lymphoma (n = 10), small cell osteosarcoma (n = 2), small cell carcinoma (n = 2) and mesenchymal chondrosarcoma (n = 2). The tumors involved limbs, axial skeleton and flat bones. Microscopically, the tumors shared similar histology, with small round cells and spindly cells arranged in diffuse sheets. The pathologic diagnosis by core needle biopsies correlated with that by surgical specimens in 84.4% (65/77) of the cases.
CONCLUSIONSSCMT represents a heterogeneous group of malignancy. Correlations with clinicoradiologic findings and application of ancillary investigations including immunohistochemistry and molecular study are important for definitive diagnosis. Pathologic diagnosis using core needle biopsies shows good results and provides useful information for surgical planning.
12E7 Antigen ; Adolescent ; Adult ; Aged ; Antigens, CD ; metabolism ; Biopsy, Large-Core Needle ; Bone Neoplasms ; diagnosis ; metabolism ; pathology ; Carcinoma, Small Cell ; diagnosis ; metabolism ; pathology ; Cell Adhesion Molecules ; metabolism ; Child ; Female ; Humans ; Lymphoma ; diagnosis ; metabolism ; pathology ; Male ; Middle Aged ; Neuroectodermal Tumors, Primitive, Peripheral ; diagnosis ; metabolism ; pathology ; Oncogene Proteins, Fusion ; metabolism ; Osteosarcoma ; diagnosis ; metabolism ; pathology ; Plasmacytoma ; diagnosis ; metabolism ; pathology ; Proto-Oncogene Protein c-fli-1 ; metabolism ; RNA-Binding Protein EWS ; metabolism ; Retrospective Studies ; Sarcoma, Ewing ; diagnosis ; metabolism ; pathology ; Vimentin ; metabolism ; Young Adult
6.Clinicopathologic features of adult granulosa cell tumor including unusual morphologic variants.
Xia LIU ; Gulinar ABULAJIANG ; Ming LIU ; Wei SANG ; Yu-qing MA ; Wei ZHANG ; Wen-tao YANG
Chinese Journal of Pathology 2013;42(1):44-45
12E7 Antigen
;
Adnexa Uteri
;
surgery
;
Adult
;
Aged
;
Aged, 80 and over
;
Antigens, CD
;
metabolism
;
Cell Adhesion Molecules
;
metabolism
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Granulosa Cell Tumor
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drug therapy
;
metabolism
;
pathology
;
surgery
;
Humans
;
Hysterectomy
;
methods
;
Inhibins
;
metabolism
;
Middle Aged
;
Neoplasm Recurrence, Local
;
Neoplasm Staging
;
Ovarian Neoplasms
;
drug therapy
;
metabolism
;
pathology
;
surgery
;
Survival Rate
;
Vimentin
;
metabolism
7.Evaluation of the expression and significance of Claudin-5 and CD99 in solid-pseudopapillary neoplasms and neuroendocrine tumors of pancreas.
Hong-yan ZHENG ; Yan-hong SHI ; Li-fang ZHANG ; Ying-zhun CHEN
Chinese Journal of Pathology 2013;42(6):372-375
OBJECTIVETo investigate the expression of endothelium tight junction protein Claudin-5 and intercellular adhesion molecule CD99 in solid-pseudopapillary neoplasms (SPN) and neuroendocrine tumors of pancreas (P-NET), and their significance in the differential diagnoses.
METHODSImmunohistochemical staining of Claudin-5 and CD99 was performed in 37 cases SPN and 21 cases of P-NET.
RESULTSMembranous Claudin-5 expression was observed in all cases of SPN but was absent in all cases of P-NET. The difference was significant (P < 0.01). In SPN, 91.9% (34/37) of the cases displayed paranuclear dot-like immunoreactivity for CD99; in contrast, 61.9% (13/21) of the cases of P-NET displayed membranous staining (P < 0.01). There was a positive association between the expression of Claudin-5 and CD99 in SPN (r = 0.421,P = 0.001).
CONCLUSIONSAlthough the macroscopic and microscopic features of SPN are quite characteristic, they may not allow confident differentiation from P-NET in all cases, especially when these characteristics are not classical. If necessary, immunostaining for Claudin-5 and CD99 can help to differentiate between these entities.
12E7 Antigen ; Adolescent ; Adult ; Aged ; Antigens, CD ; metabolism ; Carcinoma, Papillary ; metabolism ; pathology ; Cell Adhesion Molecules ; metabolism ; Child ; Claudin-5 ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Male ; Middle Aged ; Neuroendocrine Tumors ; metabolism ; pathology ; Pancreatic Neoplasms ; metabolism ; pathology ; Retrospective Studies ; Tight Junctions ; metabolism ; Young Adult
8.Pseudoangiomatous variant of spindle cell lipoma: report of a case.
Bing-wei DONG ; Xiao-yan MA ; Hui-nü HE ; Fen-juan ZHANG
Chinese Journal of Pathology 2013;42(5):343-344
12E7 Antigen
;
Antigens, CD
;
metabolism
;
Antigens, CD34
;
metabolism
;
Cell Adhesion Molecules
;
metabolism
;
Diagnosis, Differential
;
Head and Neck Neoplasms
;
metabolism
;
pathology
;
Hemangioma
;
pathology
;
Humans
;
Lipoma
;
metabolism
;
pathology
;
Liposarcoma, Myxoid
;
pathology
;
Male
;
Middle Aged
;
S100 Proteins
;
metabolism
;
Soft Tissue Neoplasms
;
metabolism
;
pathology
9.CD99 regulates redifferentiation of classical Hodgkin's lymphoma cell line L428 towards B cells.
Xinhua ZHOU ; Xueping HUANG ; Zuoping HUANG ; Zhiqiang WANG ; Tong ZHAO
Journal of Southern Medical University 2013;33(2):235-238
OBJECTIVETo explore the effect of CD99 overexpression on the morphology and differentiation-related phenotypes of classical Hodgkin's lymphoma cell line L428 and investigate the role of CD99 gene in Hodgkin/Reed-Sternberg (HRS) cell generation and transformation.
METHODSThe effect of CD99 overexpression on the cell morphology was detected by HE staining and phalloidin staining. Differentiation-related protein expressions were detected by immunocytochemistry and flow cytometry after stable transfection of CD99 gene in L428 cells.
RESULTSCD99 overexpression caused a decrease of the cell size and reorganization of the actin cytoskeleton in L428 cells. Upregulation of CD99 led to the loss of classical Hodgkin's lymphoma diagnosis marker CD30 and CD15 and the restoration of the B-cell makers of PAX5, CD19, CD79α, BCL-6, and CD10.
CONCLUSIONCD99 overexpression leads to redifferentiation of L428 cells towards B cells, suggesting that the loss of B-cell phenotype in classical Hodgkin's lymphoma is very likely a result of down-regulated CD99 expression.
12E7 Antigen ; Antigens, CD ; genetics ; B-Lymphocytes ; cytology ; Cell Adhesion Molecules ; genetics ; Cell Differentiation ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Hodgkin Disease ; genetics ; pathology ; Humans
10.Co-expression of CD99/MIC2 and ALK in anaplastic large-cell lymphoma tissues and its significance.
Lin ZHONG ; Qing-zhu WEI ; Xue-ping HUANG ; Tian-hai JI ; Feng LI ; Zhi-qiang WANG ; Xiang-zhao LI ; Jiang-huan LIU ; Tong ZHAO
Chinese Journal of Hematology 2012;33(3):173-176
OBJECTIVETo investigate co-expression of CD99/MIC2 and anaplastic lymphoma kinase (ALK) protein in anaplastic large-cell lymphoma (ALCL) tissues and Karpas 299 cells and its significance.
METHODSClinical prognoses and ALK protein expressions of 25 cases of ALCL were reviewed retrospectively, the median duration of survival was analyzed for patients with ALK(+) ALCL and ALK(-) ALCL. Histological and immunohistochemical staining were applied to other 25 cases of ALCL and paraffin-embedded tissue from human anaplastic large-cell lymphoma Karpas 299 cells to detect the protein of CD99 and ALK.
RESULTSOf former 25 cases of ALCL, median duration of survival for ALK(+) patients was 59 months, whereas 20 months for ALK(-) patients. The prognosis of ALK(+) group was better than that of ALK(-) group, survival curves of these two groups showed statistically significant (P < 0.05). CD99 was positive in 18 cases (72.0%) while negative in 7 cases (28.0%) of the latter 25 ALCL, ALK was positive in 19 cases (76.0%) while negative in 6 cases (24.0%); Of 19 ALK(+) ALCL, 16 (84.2%) cases co-expressed CD99-ALK; and in 6 ALK(-) ALCL, 2(33.3%) were CD99-ALK double negative, the expression of CD99 protein strongly correlated with that of ALK protein (P < 0.05). ALK and CD99 protein expressed in Karpas 299 cells with diffuse distribution.
CONCLUSIONSCD99 highly expressed in ALCL, and showed high rate of co-expression with ALK. CD99 protein expression could be considered as a helpful diagnostic and prognostic factor of ALCL, especially for ALK(+) ALCL.
12E7 Antigen ; Adolescent ; Adult ; Aged ; Antigens, CD ; metabolism ; Cell Adhesion Molecules ; metabolism ; Cell Line, Tumor ; Female ; Humans ; Lymphoma, Large-Cell, Anaplastic ; diagnosis ; metabolism ; Male ; Middle Aged ; Prognosis ; Receptor Protein-Tyrosine Kinases ; metabolism ; Retrospective Studies ; Young Adult

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