The immunoregulatory effects of mescenchymal stem cell (MSC) and its application have become a focused research topic in recent years. This article reviews the up-to-date research advances in the features and mechanisms of immune regulation of MSC and its application.

The immunoregulatory effects of mescenchymal stem cell (MSC) and its application have become a focused research topic in recent years.This article reviews the up-to-date research advances in the features and mechanisms of immune regulation of MSC and its application.

Correlation of blood gas analysis was made in 41 cases of young infa-nts younger than 3 months of age suffering from acute broncho -pneu-monia. Correlation of PO_2 with PCO_2 HCO_3~-, pH and BE was mace accord-dingly and with each other. Among which PO_2 and PCO_2 were the main items. Altogether 15 rairs of correl-alion in all were made.PO_2 and PCO_2 are significantly negatively correlated (P

ObjectiveTo comprehend the change of the characteristics of lifting force produced by a working space marker in process of its cavity-forming. Methods37 patients were successively operated with the surgical mode of minimally invasive video-assisted thyroidectomy (22/37 cases received a lobotomy and others un derwent a partial thyroidectomy) from January to August, 2010. Instead of hand-retraction, a mechanical armworking space marker type I ( WSM-I, MIEO Medinstr Co. Ltd, China) was applied to establish a working space. After pathway making, an interlayer-cavity above the lobe was created by the space maker and endoscopic view was properly built. Following all these steps, a simulated space making procedure was performed in a way of stepwise hook-lifting (5 mm rising per time). The lifting force ( LF)was measured during the process with a modflied force-measure device (FB-50, DESIK company, Germen). Then recorded data were assessed and analyzed statistically. Results①Ascending scope of LF in the process of entire space-forming was 0-27.5 Newton (N).②Along with hook rising, LF ascended correspondingly and 2 specific values emerged: One was 11. 2 ±3.5 N,as the lifting height approached 1.5 cm ( also a approximate position of essential space-forming ( Pe), at which themusculo-cutaneoustissuejustbecame tight) ; the other was 17.5 ± 4.3 N , as the lifting height approached 1.75 cm ( also a approximate position of maximal space-forming (Pmax), at which the musculo-cutaneous tissue appeared real tight, but not in a status of extreme tightness). ③Two types of LF ascending were found when the values transferred to a curve diagram : a palliative linearity increasing while lifting height varied from 0 to 1.5 cm (PO to Pe) and a rapid exponent-like increasing while lifting height varied from 1.5 to 1.75cm ( Pe to Pmax). ④ Dependability analyses yielded a diverse statistical outcome: negative significance of the comparison between incision length and LF value ( P ＞ 0. 05 ), and positive significance of the comparison between skin thickness and LF value ( P ＜ 0. 01 ). Conclusions①LF produced by WSM-I while establishing a working space is proper and relatively small, since the maximal value is merely 27.5N, far less than the stress produced by ordinary cosmetic skin expansion. ②The whole space-forming process can be divided into 2 stages according to the characteristic of LF ascending which correspond also separately to the “essential cavity-forming” and “the maximum cavity-forming” in the real establishing of a working space. ③Attention should be paid to the later stage since in which a rapid LF increasing occurs while the appearance of musculo-cutaneous tissue changes from “just become tight” to “real appear tight”. ④LF control, especially the fine readjustment at or about Pmax should be of necessity in individual space-forming, and then, ideal working space establishment can be archived at a pre cisely balanced LF point: maximum cavity volume acquired and minimal tissue expansion stress produced.

Following an analysis of the problems and challenges of public hospitals in Shanghai,the paper presented the approach of Shanghai Shenkang Hospital Development Center in its problem-based reform of such hospitals.The measures include promotion of capital construction,enhanced discipline human resources,and accelerated informationization,as well as overall budget management and hospital director performance appraisal,which enhanced their service capacity and operational efficiency.

ObjectiveTo explore the effects of different anesthesia approaches on hemorheology in patients with pregnancy-induced hypertension (PIH) during cesarean section.MethodsOne hundred and sixty-five patients with PIH were divided into epidural anesthesia (81 cases,control group) and combined spinal epidural anesthesia (84 cases,experiment group).The hemorheology and Apgar score were compared.Results The anesthesia and post-parturition,the levels of whole blood low shear viscosity,whole blood middle shear viscosity,whole blood high shear viscosity,erythrocyte aggregation index,plasma viscosity,fibrinogen were improved compared with those before anesthesia [experiment group:(6.3 ± 0.7 ),(6.6 ± 0.6)mPa·s vs.(8.1 ± 1.8) mPa·s,(3.8±0.6),(3.9±0.4) mPa·s vs.(5.2±0.9) mPa·s,(3.1 ±0.8),(3.2±0.7) mPa·s vs. (4.5 ± 1.2) mPa·s,3.0 ±0.4,3.1 ±0.7 vs.2.8 ±0.7,(1.2 ±0.2),(1.1 ±0.5) mPa·s vs.(1.8 ±0.6) mPa·s,(5.3 ± 0.8),(5.3 ±0.7) g/L vs.(4.7 ±0.8) g/L;control group:(7.9 ±0.8),(7.5 ±0.7)mPa·s vs.(8.2± 1.3) mPa·s,(4.5±0.4)、(4.5 ±0.4) mPa·s vs.(5.4±0.7) mPa·s,(3.7±0.8),(3.5±0.8 ) mPa· s vs. (4.6 ± 0.5 ) mPa· s,2.8 ± 0.6,2.9 ± 0.3 vs.2.7 ± 0.6,( 1.6 ± 0.4),( 1.7 ± 0.1 ) mPa· s vs.(1.9 ±0.8) mPa ·s,(5.0 ±0.9),(4.8 ±0.8) g/L vs. (4.7 ±0.5) g/L,P ＜0.05],and all the level of hemorheology in experiment group were improved compared with control group (P ＜ 0.05).There was no significant difference in 1,5 min Apgar scores between two groups (P ＞ 0.05).ConclusionThe epidural anesthesia and combined spinal epidural anesthesia can improve hemorheology of patients with PIH during cesarean section,but the combined spinal epidural anesthesia is more effective and favorable for pregnancy outcome.

Objective To investigate the effect of target diameter and target-to-background ratio (TBR) on the measurement of SUVmax [| (true SUV-SUVmax)/true SUV | × 100％,△SUVmax) in PET.Methods Six cylinders with various diameters from 5 to 29 mm were inserted in National Electrical Manufacturers Association (NEMA) NU 2-1994 phantom.The six cylinders and background of phantom were filled with18F-FDG solution.Six different TBRs (1.79,3.70,6.25,10.59,14.87 and 17.88) were obtained by changing the 18F-FDG concentration in the six cylinders.The PET data were acquired in the 2D mode,and the target's inner diameter and SUVmax were measured.The logarithmic fitting curves of △SUVmax with diameter in different TBRs using equations (y=aln(x)+b) were acquired by Microsoft excel,| a| as the influence power of diameter on △SUVmax.Statistical analysis was performed with Pearson correlation test and curve estimation utilizing SPSS 17.0.Results The Pearson correlation coefficient between △SUVmax and diameter was-0.806 (P＜0.01).△SUVmax was inversely correlated with the diameter.When the diameter (the inner diameter measured on PET images) was 4.0 mm,△SUVmax could be as high as 79.73％ with different TBRs.The Pearson correlation coefficient between the influence power of diameter on △SUVmax (| a |) and TBRs was 0.848 (P＜0.05).When TBR was 6.25,10.59 and 14.87,the corresponding |a| was similar:38.019,39.998 and 39.362,respectively.When TBR was 17.88,| a| was the highest as 43.234.When TBR was 1.79 and 3.70,|a| was much smaller:14.141 and 23.411 respectively.Conclusions The lesion diameter is inversely correlated with △SUVmax.The influence power of diameter on △SUVmax is strongly correlated with TBR.Therefore,the effect of target diameter and TBR on the measurement of SUVmax should be taken into consideration for follow-up studies.

OBJECTIVE:To establish the GC-MS fingerprint spectrum of volatile components in Danqi piantan capsule. METH-ODS:GC-MS was performed. HPLC conditions:The column was BPX 5,temperature of injection was 250 ℃,respectively;carri-er gas was He,flow rate was 1 ml/min,split injection,split ratio was 50:1 and volume injection was 0.02 μl. MS conditions:ion source was EI,bombarding energy was 70 eV,temperature of ion source and transmission line was 200 ℃ and 250 ℃,respective-ly;scan range was 40-500 amu,solvent delay of 2 min. The peak with largest chromatographic peaks areas was reference peak,com-mon peaks of 10 batches of Danqi piantan capsule were analyzed. Similarity evaluation system for chromatographic figerprint of TCM was adapted for the similarity analysis and NIST standard MS database was used to retrieve and identify the common peak. RESULTS:There were totally 13 common peaks in the 10 batches of Danqi piantan capsule with similarity degree>0.92;accord-ing to the verification,the fingerprint spectrum was a dapted for the similarity analysis and reference fingerprint of 10 batches of Danqi piantan capsule showed good consistency. The No.3 peak was Angelica sinensis unique,the No.2,4,5,7,8,12 peak was Acorus tatarinowii unique. CONCLUSIONS:The established method is specific and stable,and can provide basis for the quality as-sessment for Danqi piantan capsule.

Objective To survey the significance of specific immune response in the relationship between Sagnation the Liver-qi syndrome and the spleen Deficiency syndrome.Methods The rats with the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome were respectively corresponding and crossely treated with Radix Bupleuric dispersing the stagnated Liver-qi powder Decolion of Four Noble Drugs. Their T, B lymphocyte activity and specific immune response indexes changes (such as IgG, IgM in the serum) were respectively observed by lymphocyte transformation test and immunoturbidimetry. Results There were no notable changes about the IgG level among all kinds of groups. The IgM levels in the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome decreased, especially in the Liver-qi Stagnation syndrome (P0 05), Decolion of Four Noble Drugs could increase the IgM level of the patients with the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome (P0 05); The T lymphocyte transformation rate of the Spleen Deficiency syndrome obviously decreased, and Decolion of Four Noble Drugs could recover it to the normal level (P0 05). The rate of B lymphocyte transformation the Liver-qi Stagnation syndrome's also showed the increasing tenderly (P0 05). Conclusions The relationship between the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome could occur in the phase of antigen presentation, especially in the phase of the T H cell activation stimulated by antigen. Radix Bupleuric dispersing the stagnated Liver-qi powder could enhance T, B lymphocyte activities by reverse the Liver-qi Stagnation syndrome,and Decolion of Four Noble Drugs could enhance T lymphocyte by improving the Spleen Deficiency syndrome.

[Objective]To investigate the possibility of stable expression of VEGF~65 in human bone marrow stromal cell.[Method]hBMSc were divided into transfection group, empty vector group and control group, hBMSc were infected with Ad-VEGF165(MOI=20 pfu). The hBMSc proliferation and transfection efficiency were confirmed by fluorescence microscope.The expression of VEGF165 gene of cultured hBMSc transfected with VEGF165 gene was assayed by RT-PCR, ELISA and Western blot.[Result]VEGF165 gene was amplified with RT-PCR.GAPDH gene(internal control) was located at 549 bp in the three groups, VEGF165 gene was located at 500 bp in transfection group but not in the other groups. ELISA assay showed that the expression had significant difference in the transfection group compared with the empty vector group and control group (P